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Open AccessArticle

Selection and Micropropagation of an Elite Melatonin Rich Tulsi (Ocimum sanctum L.) Germplasm Line

1
Department of Plant Agriculture, Gosling Research Institute for Plant Preservation, University of Guelph, Guelph, ON N1G 2W1, Canada
2
I.K. Barber Faculty of Science, University of British Columbia, Kelowna, BC V1V 1V7, Canada
*
Author to whom correspondence should be addressed.
Agronomy 2021, 11(2), 207; https://doi.org/10.3390/agronomy11020207
Received: 13 December 2020 / Revised: 9 January 2021 / Accepted: 19 January 2021 / Published: 22 January 2021
(This article belongs to the Special Issue Role of Plant Tissue Culture in Agricultural Research and Production)
Tulsi (Ocimum sanctum L.) is a sacred plant of medicinal and spiritual significance in many cultures. Medicinal properties of Tulsi are ascribed to its phytochemicals with antioxidant capabilities. The current study was undertaken to screen a large seed population of Tulsi to select germplasm lines with high antioxidant potential and to standardize protocols for micropropagation and biomass production to produce a phytochemically consistent crop. A total of 80 germplasm lines were established under in vitro conditions and screened for their antioxidant potential determined with the 2,2-diphenyl-1-picrylhydrazyl (DPPH) bioassay. The micropropagation of a selected line, named Vrinda, was established using nodal cultures grown on Murashige and Skoog medium containing benzylaminopurine (1.1 µM), gibberellic acid (0.3 µM), and activated charcoal (0.6%). The antioxidant phytohormones melatonin and serotonin were quantified in the field and greenhouse grown tissues of Vrinda and melatonin levels were found to be consistent in both conditions with higher serotonin levels under field conditions. This integrated approach combining the in vitro selection and propagation offers potential applications in the development of safe, effective, and novel natural health products of Tulsi, and many other medicinal plant species. View Full-Text
Keywords: Tulsi; in vitro culture; germplasm; micropropagation; antioxidant; melatonin Tulsi; in vitro culture; germplasm; micropropagation; antioxidant; melatonin
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MDPI and ACS Style

Shukla, M.R.; Kibler, A.; Turi, C.E.; Erland, L.A.E.; Sullivan, J.A.; Murch, S.J.; Saxena, P.K. Selection and Micropropagation of an Elite Melatonin Rich Tulsi (Ocimum sanctum L.) Germplasm Line. Agronomy 2021, 11, 207. https://doi.org/10.3390/agronomy11020207

AMA Style

Shukla MR, Kibler A, Turi CE, Erland LAE, Sullivan JA, Murch SJ, Saxena PK. Selection and Micropropagation of an Elite Melatonin Rich Tulsi (Ocimum sanctum L.) Germplasm Line. Agronomy. 2021; 11(2):207. https://doi.org/10.3390/agronomy11020207

Chicago/Turabian Style

Shukla, Mukund R.; Kibler, Annaliese; Turi, Christina E.; Erland, Lauren A.E.; Sullivan, J. A.; Murch, Susan J.; Saxena, Praveen K. 2021. "Selection and Micropropagation of an Elite Melatonin Rich Tulsi (Ocimum sanctum L.) Germplasm Line" Agronomy 11, no. 2: 207. https://doi.org/10.3390/agronomy11020207

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