Selection and Micropropagation of an Elite Melatonin Rich Tulsi (Ocimum sanctum L.) Germplasm Line
Abstract
:1. Introduction
2. Materials and Methods
2.1. Plant Materials and Germplasm Line Development
2.2. Determination of Antioxidant Potential Using DPPH Bioassay
2.3. Culture Establishment and Propagation
2.4. Shoot Multiplication
2.5. Rooting and Acclimatization in the Greenhouse
2.6. Folin-Ciocalteu Phenolic Assay
2.7. Detection and Quantification of Neurotransmitters
2.8. Statistical Analysis
3. Results
4. Discussion
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Compound | Transition | Cone Voltage | Collision Voltage |
---|---|---|---|
Serotonin | 177 > 160 | 45 | 10 |
177 > 115 | 27 | ||
Melatonin | 233 > 159 | 30 | 23 |
233 > 174 | 15 |
Source | Melatonin (ng/g) | Serotonin (ng/g) |
---|---|---|
Wildtype | 42.8 ± 4.95 b | 1684.7 ± 82.04 a |
Vrinda | 66.4 ± 16.5 a | 1305.1 ± 139.43 b |
Source/Plant Parts | Leaf | Root | ||
---|---|---|---|---|
MEL (ng/g) | SER (ng/g) | MEL (ng/g) | SER (ng/g) | |
Field | 327.17 a | 685.71 a | 365.05 a | 605.26 a |
Greenhouse | 341.83 a | 497.95 a | 378.64 a | 501.79 a |
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Shukla, M.R.; Kibler, A.; Turi, C.E.; Erland, L.A.E.; Sullivan, J.A.; Murch, S.J.; Saxena, P.K. Selection and Micropropagation of an Elite Melatonin Rich Tulsi (Ocimum sanctum L.) Germplasm Line. Agronomy 2021, 11, 207. https://doi.org/10.3390/agronomy11020207
Shukla MR, Kibler A, Turi CE, Erland LAE, Sullivan JA, Murch SJ, Saxena PK. Selection and Micropropagation of an Elite Melatonin Rich Tulsi (Ocimum sanctum L.) Germplasm Line. Agronomy. 2021; 11(2):207. https://doi.org/10.3390/agronomy11020207
Chicago/Turabian StyleShukla, Mukund R., Annaliese Kibler, Christina E. Turi, Lauren A. E. Erland, J. Alan Sullivan, Susan J. Murch, and Praveen K. Saxena. 2021. "Selection and Micropropagation of an Elite Melatonin Rich Tulsi (Ocimum sanctum L.) Germplasm Line" Agronomy 11, no. 2: 207. https://doi.org/10.3390/agronomy11020207