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13C-Enrichment of Urinary Uric Acid after l-[Ring-2-13C]Histidine Dose in Adult Humans

Department of Nutrition Sciences, University of Alabama at Birmingham, Birmingham, AL 35294, USA
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Nutrients 2015, 7(1), 697-705; https://doi.org/10.3390/nu7010697
Received: 13 November 2014 / Revised: 6 January 2015 / Accepted: 15 January 2015 / Published: 20 January 2015
We determined whether ring-2 carbon of histidine is folate-dependently transferred to carbons 8 (C8) and/or 2 (C2) in urinary uric acid in humans. Two adults collected each urine void for four days. Aliquots of urine for the first day were used for baseline values; then the subjects ingested 0.7 g (3.3 mmol) of l-[ring-2-13C]histidine and collected urine for three experimental days. Aliquots were analyzed for percentage 13C-content at C2 and C8 by a liquid-chromatography-mass spectrometry method. Percentage enrichment was determined by subtracting time-of-day paired baseline percentage 13C-content from experimental percentage 13C-content for each void. C2 was predominantly 13C-enriched in the majority of voids. The percentage enrichments at C2 for two subjects were 0.14 (±0.028 [SEM], n = 26) and 0.18 (±0.049, n = 21), whereas at C8, they were 0.008 (±0.006) and −0.005 (±0.008), respectively. The mean C2-enrichments were significantly greater than zero (p < 0.01), whereas those of C8 were not (p > 0.2). The enrichment had a diurnal rhythm peaking in the morning. Our results may be useful in the estimation of the timing for the administration of drugs that interfere with purine nucleotide biosynthesis in the treatment of cancer and autoimmune disease. View Full-Text
Keywords: C2 enrichment; uric acid; folate metabolism; humans; purine nucleotide biosynthesis; [ring-2-13C]histidine C2 enrichment; uric acid; folate metabolism; humans; purine nucleotide biosynthesis; [ring-2-13C]histidine
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Tamura, T.; Baggott, J.E. 13C-Enrichment of Urinary Uric Acid after l-[Ring-2-13C]Histidine Dose in Adult Humans. Nutrients 2015, 7, 697-705.

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