Next Article in Journal
The Tobacco β-Cembrenediol: A Prostate Cancer Recurrence Suppressor Lead and Prospective Scaffold via Modulation of Indoleamine 2,3-Dioxygenase and Tryptophan Dioxygenase
Previous Article in Journal
Diabetes and Familial Hypercholesterolemia: Interplay between Lipid and Glucose Metabolism
Article

Vitamin K2 Modulates Mitochondrial Dysfunction Induced by 6-Hydroxydopamine in SH-SY5Y Cells via Mitochondrial Quality-Control Loop

1
Institute of Intelligent Machines, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei 230031, China
2
Science Island Branch of Graduate, University of Science and Technology of China, Hefei 230026, China
3
Anhui Key Laboratory of Environmental Toxicology and Pollution Control Technology, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei 230031, China
4
CAS (Hefei) Institute of Technology Innovation Co., Ltd., Hefei 230088, China
*
Authors to whom correspondence should be addressed.
Academic Editors: Lucian Hritcu and Omayma A. Eldahshan
Nutrients 2022, 14(7), 1504; https://doi.org/10.3390/nu14071504
Received: 11 March 2022 / Revised: 31 March 2022 / Accepted: 2 April 2022 / Published: 4 April 2022
Vitamin K2, a natural fat-soluble vitamin, is a potent neuroprotective molecule, owing to its antioxidant effect, but its mechanism has not been fully elucidated. Therefore, we stimulated SH-SY5Y cells with 6-hydroxydopamine (6-OHDA) in a proper dose-dependent manner, followed by a treatment of vitamin K2. In the presence of 6-OHDA, cell viability was reduced, the mitochondrial membrane potential was decreased, and the accumulation of reactive oxygen species (ROS) was increased. Moreover, the treatment of 6-OHDA promoted mitochondria-mediated apoptosis and abnormal mitochondrial fission and fusion. However, vitamin K2 significantly suppressed 6-OHDA-induced changes. Vitamin K2 played a significant part in apoptosis by upregulating and downregulating Bcl-2 and Bax protein expressions, respectively, which inhibited mitochondrial depolarization, and ROS accumulation to maintain mitochondrial structure and functional stabilities. Additionally, vitamin K2 significantly inhibited the 6-OHDA-induced downregulation of the MFN1/2 level and upregulation of the DRP1 level, respectively, and this enabled cells to maintain the dynamic balance of mitochondrial fusion and fission. Furthermore, vitamin K2 treatments downregulated the expression level of p62 and upregulated the expression level of LC3A in 6-OHDA-treated cells via the PINK1/Parkin signaling pathway, thereby promoting mitophagy. Moreover, it induced mitochondrial biogenesis in 6-OHDA damaged cells by promoting the expression of PGC-1α, NRF1, and TFAM. These indicated that vitamin K2 can release mitochondrial damage, and that this effect is related to the participation of vitamin K2 in the regulation of the mitochondrial quality-control loop, through the maintenance of the mitochondrial quality-control system, and repair mitochondrial dysfunction, thereby alleviating neuronal cell death mediated by mitochondrial damage. View Full-Text
Keywords: vitamin K2; apoptosis; ROS; mitochondrial dysfunction; mitophagy; mitochondrial biogenesis; mitochondrial quality-control loop; PINK1/Parkin signaling pathway vitamin K2; apoptosis; ROS; mitochondrial dysfunction; mitophagy; mitochondrial biogenesis; mitochondrial quality-control loop; PINK1/Parkin signaling pathway
Show Figures

Figure 1

MDPI and ACS Style

Tang, H.; Zheng, Z.; Wang, H.; Wang, L.; Zhao, G.; Wang, P. Vitamin K2 Modulates Mitochondrial Dysfunction Induced by 6-Hydroxydopamine in SH-SY5Y Cells via Mitochondrial Quality-Control Loop. Nutrients 2022, 14, 1504. https://doi.org/10.3390/nu14071504

AMA Style

Tang H, Zheng Z, Wang H, Wang L, Zhao G, Wang P. Vitamin K2 Modulates Mitochondrial Dysfunction Induced by 6-Hydroxydopamine in SH-SY5Y Cells via Mitochondrial Quality-Control Loop. Nutrients. 2022; 14(7):1504. https://doi.org/10.3390/nu14071504

Chicago/Turabian Style

Tang, Hengfang, Zhiming Zheng, Han Wang, Li Wang, Genhai Zhao, and Peng Wang. 2022. "Vitamin K2 Modulates Mitochondrial Dysfunction Induced by 6-Hydroxydopamine in SH-SY5Y Cells via Mitochondrial Quality-Control Loop" Nutrients 14, no. 7: 1504. https://doi.org/10.3390/nu14071504

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop