Lower Muscle and Blood Lactate Accumulation in Sickle Cell Trait Carriers in Response to Short High-Intensity Exercise
Abstract
:1. Introduction
2. Methods
2.1. Subjects
2.2. Experimental Design
2.2.1. Inclusion (V1)
2.2.2. Incremental Exercise Test to Exhaustion (V2)
2.2.3. Short High-Intensity Exercise Bout (V3)
2.3. Blood Lactate and Glucose Concentrations and Their Time-Courses during Recovery
2.4. Muscle Analyses
2.5. Statistical Analysis
3. Results
3.1. Blood Lactate Response to Short Supramaximal Exercise
3.2. Muscle pH Regulation, Lactate Transport, Metabolite Concentrations and Isoforms of Lactate Dehydrogenase
3.3. Blood Lactate and Glucose Kinetics during Recovery
4. Discussion
4.1. Metabolic Response to Short Supramaximal Exercise in SCT Carriers
4.2. Postexercise Blood Lactate Kinetics and Its Relation with Glycemia
4.3. Effects of α-Thalassemia
4.4. Clinical Relevance and Consequences on High-Intensity Exercise Performance
4.5. Nutritional and Metabolic Flexibility Perspectives/Hypotheses
4.6. Limitations and Perspectives
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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CON (n = 15) | SCT (n = 15) | p Value | |
---|---|---|---|
Anthropometric and physiological characteristics | |||
Age (year) | 24 (2) | 23 (2) | 0.052 |
Body mass (kg) | 66 (5) | 69 (6) | 0.151 |
Pmax (W) | 210 (170–241) | 210 (140–245) | 0.475 |
Pmax (W·kg−1) | 3.02 (0.36) | 3.01 (0.40) | 0.920 |
DEE (kJ·day−1) | 10,868 (1474) | 11,664 (1321) | 0.131 |
Hemoglobin and hematological data | |||
HbS (%) | not present | 34.3 (3.6) | na |
Hct (%) | 43.0 (2.7) | 43.2 (2.8) | 0.826 |
MCV (fL) | 84.71 (5.37) | 80.00 (3.87) | 0.010 |
MCH (pg) | 27.29 (2.08) | 25.93 (1.39) | 0.044 |
MCHC (g·dL−1) | 32.17 (0.67) | 32.33 (0.56) | 0.483 |
RBC (M·µL−1) | 5.09 (0.42) | 5.42 (0.45) | 0.053 |
CON (n = 15) | SCT (n = 15) | p Value | |
---|---|---|---|
Blood lactate concentrations | |||
[lactate]b(r) (mmol·L−1) | 1.356 (0.336) | 1.301 (0.323) | 0.648 |
[lactate]b(0) (mmol·L−1) | 8.59 (1.40) [14] | 7.08 (1.57) | 0.011 |
Bicarbonate-dependent muscle pH regulation mechanisms | |||
CAII (a.u.) | 1.28 (0.40) [13] | 1.43 (0.36) [14] | 0.317 |
CAIII (a.u.) | 1.03 (0.34) [13] | 1.05 (0.24) [13] | 0.857 |
NBC (a.u.) | 3.98 (0.67) [14] | 3.95(1.06) [13] | 0.928 |
Sarcolemmal H+ transport | |||
MCT1 (a.u.) | 2.14 (0.53) [14] | 2.33 (0.61) [13] | 0.392 |
MCT4 (a.u.) | 1.18 (1.75–4.81) [14] | 2.70 (1.34–5.78) [14] | 0.006 |
Muscle metabolite concentrations | |||
[lactate]m(0) (mmol·kg−1 d.m.) | 132 (95–201) [13] | 113 (83–130) | 0.022 |
[pyruvate]m(0) (mmol·kg−1 d.m.) | 1.85 (0.63–5.76) [13] | 2.07 (1.13–3.59) | 0.914 |
[lactate]m(0)/[pyruvate]m ratio | 60.7 (26.3–225.5) [13] | 55.2 (31.5–99.3) | 0.440 |
[ATP]m(0) (mmol·kg−1 d.m.) | 14.4 (9.0–18.3) [13] | 12.6 (9.6–21.9) | 0.908 |
[ADP]m(0) (mmol·kg−1 d.m.) | 6.0 (2.60–9.10) [13] | 6.70 (3.2–10.3) | 0.903 |
[ATP]m/[ADP]m(0) | 2.73 (1.22–4.38) [13] | 1.96 (1.30–4.56) | 0.339 |
LDH isoform proportions | |||
M-LDH (%) | 0.81 (0.71–0.84) [13] | 0.81 (0.63–0.88) | 0.610 |
H-LDH (%) | 0.19 (0.16–0.29) [13] | 0.19 (0.12–0.37) | 0.610 |
β2-adrenergic receptors | |||
β2AR (a.u.) | 0.87 (0.13–1.60) [14] | 0.25 (0.08–1.53) [11] | 0.021 |
CON (n = 15) | SCT (n = 15) | p Value | |
---|---|---|---|
Blood lactate kinetics parameters | |||
γ1 (min−1) | 0.207 (0.086) [14] | 0.227 (0.104) | 0.586 |
γ2 (min−1) | 0.045 (0.011) [14] | 0.061 (0.022) | 0.020 |
[lactate]bpeak (mmol·L−1) | 12.1 (1.7) [14] | 10.4 (1.6) | 0.009 |
Cross-over point of blood glucose and lactate concentrations | |||
Concentration (mmol·L−1) | 5.21 (0.64) [14] | 4.98 (0.34) | 0.242 |
Time into recovery (min) | 36.5 (26.3–66.9) [14] | 30.8 (22.0–36.1) | 0.012 |
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Messonnier, L.A.; Oyono-Enguéllé, S.; Vincent, L.; Dubouchaud, H.; Chatel, B.; Sanchez, H.; Malgoyre, A.; Martin, C.; Galactéros, F.; Bartolucci, P.; et al. Lower Muscle and Blood Lactate Accumulation in Sickle Cell Trait Carriers in Response to Short High-Intensity Exercise. Nutrients 2022, 14, 501. https://doi.org/10.3390/nu14030501
Messonnier LA, Oyono-Enguéllé S, Vincent L, Dubouchaud H, Chatel B, Sanchez H, Malgoyre A, Martin C, Galactéros F, Bartolucci P, et al. Lower Muscle and Blood Lactate Accumulation in Sickle Cell Trait Carriers in Response to Short High-Intensity Exercise. Nutrients. 2022; 14(3):501. https://doi.org/10.3390/nu14030501
Chicago/Turabian StyleMessonnier, Laurent A., Samuel Oyono-Enguéllé, Lucile Vincent, Hervé Dubouchaud, Benjamin Chatel, Hervé Sanchez, Alexandra Malgoyre, Cyril Martin, Frédéric Galactéros, Pablo Bartolucci, and et al. 2022. "Lower Muscle and Blood Lactate Accumulation in Sickle Cell Trait Carriers in Response to Short High-Intensity Exercise" Nutrients 14, no. 3: 501. https://doi.org/10.3390/nu14030501