Review Reports

Round 1

Reviewer 1 Report

The authors did a nice study. But would suggest the authors to add a paragraph in their introduction that in current time NSAIDS are well known for treatment of high fat diet lipid disorder, oxidative stress and inflammation but machine learning and artificial intelligence has reported that NSAIDS cause a lot of drug drug interactions (Datta, A.; Flynn, N.R.; Barnette, D.A.; Woeltje, K.F.; Miller, G.P.; Swamidass, S.J. Machine learning liver-injuring drug interactions with non-steroidal anti-inflammatory drugs (NSAIDs) from a retrospective electronic health record (EHR) cohort. PLoS Comput. Biol. 2021, 17, e1009053.)so to avoid this interactions herbal therapy is best in the up coming years.

 

 

Author Response

Reviewer #1:

The authors did a nice study. But would suggest the authors to add a paragraph in their introduction that in current time NSAIDS are well known for treatment of high fat diet lipid disorder, oxidative stress and inflammation but machine learning and artificial intelligence has reported that NSAIDS cause a lot of drug drug interactions (Datta, A.; Flynn, N.R.; Barnette, D.A.; Woeltje, K.F.; Miller, G.P.; Swamidass, S.J. Machine learning liver-injuring drug interactions with non-steroidal anti-inflammatory drugs (NSAIDs) from a retrospective electronic health record (EHR) cohort. PLoS Comput. Biol. 2021, 17, e1009053.). So to avoid this interactions herbal therapy is best in the upcoming year.

 Response: We thank the reviewer for the suggestion. We have added “Non-steroidal anti-inflammatory drugs (NSAIDs) are widely used in metabolic disorders and inflammation because of their analgesic, anti-inflammatory and antipyretic effects, but main adverse effects also existed such as drug-drug interactions, and organ damage, highlighting the need and importance of complementary and alternative medicine [1-3]” in the introduction.

References

1. Seebacher; Zeigerer A.; Kory N.; Krahmer N. Hepatic lipid droplet homeostasis and fatty liver disease. Semin. Cell Dev. Biol. 2020, 108, 72-81
2. Bindua S.; Mazumderb S.; Bandyopadhyay U. Non-steroidal anti-inflammatory drugs (NSAIDs) and organ damage: A current perspective. Pharmacol. 2020, 180, 114147.
3. Datta A.; Flynn N.R.; Barnette D.A.; Woeltje K.F.; Miller G.P.; Swamidass S.J. Machine learning liver-injuring drug interactions with non-steroidal anti-inflammatory drugs (NSAIDs) from a retrospective electronic health record (EHR) cohort. PLoS. Comput. Biol. 2021, 17, e1009053.

Reviewer 2 Report

Dear editor,

I reviewed the Manuscript n. nutrients-1889794

The subject of the paper is interesting,  I recommend for acceptance after minor revision.

The objective of the Authors was to  investigate the composition of Flammulina velutipes (FV) mycorrhizae (by-products of FV), and its effects on high fat diet (HFD)-induced lipid metabolism disorder, by measuring lipid profiles, oxidative stress, and inflammatory cytokines in plasma,  liver and in perirenal adipose tissue (PAT) of mice. The results of this study indicated the FV mycorrhizae could attenuate HFD-induced metabolic disorder, oxidative stress and inflammation both in the liver and PAT of mice.

 

Overall, the quality of the work is quite good. Author treated the data with proper methodology. However, there are many typing errors in the text. Moreover, the Introduction could provide a more extensive background.  In the Materials e Methods section the Authors, during the illustration of some types of analysis, refer to follow the instructions of Nanjing Jiancheng Bioengineering Institute, but there are no bibliographic references to support the methodologies used.

Some comments are listed below:

 

Line 16… high fat diet (HFD)-induced lipid profile… what do the author mean?

Lines 47-49   this sentence is unclear, I suggest rephrasing it.

Line 219 …Polysaccharides has three characteristics absorptions…peaks…I believe...

Line 234 …the mice body weight

Lines 470-478 ….this part is not relevant to the discussion of the results obtained.

 

Author Response

There are many typing errors in the text. Moreover, the Introduction could provide a more extensive background. In the Materials and Methods section the Authors, during the illustration of some types of analysis, refer to follow the instructions of Nanjing Jiancheng Bioengineering Institute, but there are no bibliographic references to support the methodologies used.

Response: We thank the reviewer for the suggestion. We have carefully checked our manuscript and made some corrections in the revision. Also, we added non-steroidal anti-inflammatory drugs (NSAIDs) in the introduction and methods references as our previous description.

Reference

1. Luo Z.; Xu X.; Zhao S.; Sho T.; Luo W.; Zhang J.; Xu W.; Hon K.; Xu J. Inclusion of microbe-derived antioxidant during pregnancy and lactation attenuates high-fat diet-induced hepatic oxidative stress, lipid disorders, and NLRP3 inflammasome in mother rats and offspring. Food Nutr. Res. 2019, 63. 10.29219/fnr.v63.3504.
2. Luo Z.; Zhu W.; Guo Q.; Luo W.; Zhang J.; Xu W.; Xu J. Weaning induced hepatic oxidative stress, apoptosis, and aminotransferases through MAPK signaling pathways in piglets. Med. Cell. Longev. 2016, 20164768541.

 

Line 16… high fat diet (HFD)-induced lipid profile… what do the author mean?

Response: We thank the reviewer for the suggestion. We have corrected “lipid profile” into “lipid disorder” and “lipid metabolism” in the abstract.

 

Lines 47-49   this sentence is unclear, I suggest rephrasing it.

Response: We thank the reviewer for the suggestion. We have corrected “Furthermore, the stem or mycorrhizae of mushrooms showed different chemical components and structural characteristics such as polysaccharides conjugation, solubility and antioxidant activities compared with the caps of mushroom, resulting in difference of their absorption and metabolism” in the introduction.

 

Line 219 …Polysaccharides has three characteristics absorptions…peaks…I believe...

Response: We thank the reviewer for the suggestion. We have added “peaks” in the revision.

 

Line 234 …the mice body weight

Response: We thank the reviewer for the suggestion. We have corrected them in the revision.

 

Lines 470-478 ….this part is not relevant to the discussion of the results obtained.

Response: We thank the reviewer for the suggestion. We did not make any change about this part. The results of FV mycorrhizae composition determined by untargeted metabolomics were listed in the supplemental files. We hope to make some direct links between the composition of FV mycorrhizae and plasma differential metabolites, which could help to explain the specific ingredients of FV mycorrhizae in regulating metabolism and immune response. Although we did not find the direct links, it may provide some ideas for future research.

Round 2

Reviewer 1 Report

Accept the manuscript in the current form