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Article

Exercise Training-Induced PPARβ Increases PGC-1α Protein Stability and Improves Insulin-Induced Glucose Uptake in Rodent Muscles

by 1, 2,†, 3,* and 2,4,*
1
Department of Taekwondo, College of Physical Education, Keimyung University, Daegu 42601, Korea
2
Division of Geriatrics and Nutritional Sciences, Department of Medicine, Washington University School of Medicine, St. Louis, MO 63110, USA
3
Department of Physical Education, College of Physical Education, Keimyung University, Daegu 42601, Korea
4
Department of Physiology, College of Medicine, Yeungnam University, Daegu 42415, Korea
*
Authors to whom correspondence should be addressed.
He passed away. (He had used email address: [email protected]).
Nutrients 2020, 12(3), 652; https://doi.org/10.3390/nu12030652
Received: 31 January 2020 / Revised: 20 February 2020 / Accepted: 25 February 2020 / Published: 28 February 2020
(This article belongs to the Special Issue Nutrition for Human Health, Performance and Recovery)
This study aimed to investigate the long-term effects of training intervention and resting on protein expression and stability of peroxisome proliferator-activated receptor β/δ (PPARβ), peroxisome proliferator-activated receptor gamma coactivator 1-α (PGC1α), glucose transporter type 4 (GLUT4), and mitochondrial proteins, and determine whether glucose homeostasis can be regulated through stable expression of these proteins after training. Rats swam daily for 3, 6, 9, 14, or 28 days, and then allowed to rest for 5 days post-training. Protein and mRNA levels were measured in the skeletal muscles of these rats. PPARβ was overexpressed and knocked down in myotubes in the skeletal muscle to investigate the effects of swimming training on various signaling cascades of PGC-1α transcription, insulin signaling, and glucose uptake. Exercise training (Ext) upregulated PPARβ, PGC-1α, GLUT4, and mitochondrial enzymes, including NADH-ubiquinone oxidoreductase (NUO), cytochrome c oxidase subunit I (COX1), citrate synthase (CS), and cytochrome c (Cyto C) in a time-dependent manner and promoted the protein stability of PPARβ, PGC-1α, GLUT4, NUO, CS, and Cyto C, such that they were significantly upregulated 5 days after training cessation. PPARβ overexpression increased the PGC-1α protein levels post-translation and improved insulin-induced signaling responsiveness and glucose uptake. The present results indicate that Ext promotes the protein stability of key mitochondria enzymes GLUT4, PGC-1α, and PPARβ even after Ext cessation. View Full-Text
Keywords: exercise training; protein stability; PPARβ; PGC-1α; GLUT4; Mitochondria; skeletal muscle exercise training; protein stability; PPARβ; PGC-1α; GLUT4; Mitochondria; skeletal muscle
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MDPI and ACS Style

Park, J.-S.; Holloszy, J.O.; Kim, K.; Koh, J.-H. Exercise Training-Induced PPARβ Increases PGC-1α Protein Stability and Improves Insulin-Induced Glucose Uptake in Rodent Muscles. Nutrients 2020, 12, 652. https://doi.org/10.3390/nu12030652

AMA Style

Park J-S, Holloszy JO, Kim K, Koh J-H. Exercise Training-Induced PPARβ Increases PGC-1α Protein Stability and Improves Insulin-Induced Glucose Uptake in Rodent Muscles. Nutrients. 2020; 12(3):652. https://doi.org/10.3390/nu12030652

Chicago/Turabian Style

Park, Ju-Sik, John O. Holloszy, Kijin Kim, and Jin-Ho Koh. 2020. "Exercise Training-Induced PPARβ Increases PGC-1α Protein Stability and Improves Insulin-Induced Glucose Uptake in Rodent Muscles" Nutrients 12, no. 3: 652. https://doi.org/10.3390/nu12030652

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