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Microbiology Research, Volume 17, Issue 5

2026 May - 16 articles

Cover Story: In this review, we examine how Escherichia coli, one of the most versatile microbial platforms in biotechnology, can be systematically engineered to overproduce these compounds through the shikimate pathway, a metabolic route absent in mammals but conserved across bacteria, fungi, and plants. We discuss strategies organized around the key metabolic nodes chorismate, L-Tyrosine, L-Phenylalanine, and L-Tryptophan, covering classical metabolic engineering approaches and emerging synthetic biology tools, including CRISPR-based regulation, biosensor-driven dynamic control, membrane engineering, and synthetic microbial consortia. This integrated perspective highlights both the remarkable progress achieved and the critical challenges that remain, offering a roadmap for developing efficient, scalable microbial cell factories for aromatic-compound bioproduction. View this paper
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Articles (16)

  • Article
  • Open Access
1,788 Views
11 Pages

Multidrug-Resistant Bacterial Strains in Northern Mexico: Clinical Impact and Vitek 2-Based Characterization

  • Rafael Martínez-Miranda,
  • Fernando A. Solis-Dominguez,
  • Aseneth Herrera-Martínez,
  • Conrado Garcia-Gonzalez,
  • Jonathan Isaac Arauz Cabrera,
  • Rafael Iván Ayala Figueroa and
  • Carlos Vidal Montiel Castañeda

Antibiotic resistance remains a major public health concern, particularly in regions with high rates of hospital- and community-acquired infections. This study aimed to quantify multidrug-resistant (MDR) bacterial strains in northern Mexico and to id...

  • Article
  • Open Access
1 Citations
1,386 Views
13 Pages

Prevalence and Genotyping of Human Papillomavirus in Oral Squamous Cell Carcinoma, Oral Potentially Malignant Disorders, and Healthy Oral Mucosa: A Cross-Sectional Study

  • Teodora Bolyarova,
  • Pavel Stanimirov,
  • Ivo Sirakov,
  • Emilia Naseva,
  • Bilyana Sirakova,
  • Konstantin Stamatov and
  • Samuil Dzhenkov

Background and Objectives: This study aimed to detect and genotype human papillomavirus (HPV) in tissues from oral squamous cell carcinoma (OSCC), oral potentially malignant disorders (OPMD), and healthy individuals. Materials and Methods: The study...

(This article belongs to the Section Medical and Veterinary Microbiology)
  • Article
  • Open Access
2 Citations
658 Views
15 Pages

Human herpesvirus 6 (HHV-6) typically remains latent but can reactivate during immunosuppression caused by chemotherapy, potentially driving immune dysregulation. The NLRP3 inflammasome is a critical innate immune complex mediating pro-inflammatory s...

(This article belongs to the Special Issue Host–Microbe Interactions in Health and Disease)
  • Article
  • Open Access
735 Views
17 Pages

Bioactivity of Pod and Seed Extracts from Leucaena leucocephala, Prosopis laevigata, and Pithecellobium dulce Collected in Oaxaca, Mexico

  • Jesús Andres Morales-López,
  • Teodulfo Aquino-Bolaños,
  • Angélica Bautista-Cruz,
  • Tamara Aquino-Aguilar,
  • Edgar García-Sánchez,
  • Tlacaelel Aquino-López and
  • Keyla Cruz-García

Guaje (Leucaena leucocephala), mezquite (Prosopis laevigata), and guamuchil (Pithecellobium dulce) are leguminous trees distributed throughout southeastern Mexico. Their pods and seeds constitute the main agroecological residues and represent a natur...

(This article belongs to the Special Issue Advances in Plant–Pathogen Interactions)
  • Article
  • Open Access
607 Views
13 Pages

Real-Time Fluorescence Imaging Platform for Screening Arbuscular Mycorrhizal Fungi by Hyphal Transport Kinetics

  • Guangle Zhang,
  • Lixue Yuan,
  • Yongxin Zhang,
  • Xiaohang Wang,
  • Li Zhang,
  • Xinyuan Zhang,
  • Ruxue Chen,
  • Zhuangzhuang Wang,
  • Bo Yu and
  • Yonghua Wang

Arbuscular mycorrhizal (AM) fungi form mutualistic symbioses with about 80% of land plants and play a key role in improving host inorganic phosphate (Pi), nitrogen, and water acquisition. Traditional AM fungi research relies on field trials, compartm...

(This article belongs to the Topic New Challenges on Plant–Microbe Interactions)
  • Article
  • Open Access
687 Views
19 Pages

Optimization of IAA Production by Halotolerant Vreelandella titanicae J113 Through Fermentation Process Engineering with Response Surface Methodology

  • Dilbar Tursun,
  • Zulhumar Yakup,
  • Huifang Bao,
  • Faqiang Zhan,
  • Yingwu Shi,
  • Hongmei Yang,
  • Jiusheng Sun,
  • Shijie Fang and
  • Ning Wang

Soil salinization is a significant environmental factor limiting agricultural production. Developing salt–alkali-tolerant microbial resources is important for the improvement of saline–alkali land. Plant growth-promoting rhizobacteria sti...

  • Review
  • Open Access
1,723 Views
18 Pages

Aromatic compounds derived from the shikimate (SHK) pathway constitute a diverse class of high-value molecules with applications in the pharmaceutical, food, cosmetic, and chemical industries. In microbial systems, particularly Escherichia coli, this...

  • Article
  • Open Access
1,100 Views
13 Pages

Molecular Epidemiology of Drug-Resistant Mycobacterium tuberculosis: Mutation Profiles and Resistance Associations

  • Mandlenkosi Manika,
  • Lindiwe Modest Faye,
  • Ntandazo Dlatu and
  • Mojisola Clara Hosu

Background: The global burden of drug-resistant Mycobacterium tuberculosis continues to threaten tuberculosis control efforts, largely due to the emergence and transmission of resistance-associated genetic mutations. Molecular epidemiology provides c...

(This article belongs to the Topic Multidrug Resistance Across Pathogens: Fungi, Bacteria, Parasites, and Viruses)
  • Systematic Review
  • Open Access
1 Citations
1,093 Views
22 Pages

Molecular and Proteomic Determinants of Trypanosoma cruzi Adaptation Within Triatomine Vectors: Insights from Current Experimental Models

  • Jessy T. Santana,
  • Berenice González-Rete,
  • Elia Torres-Gutiérrez,
  • Juliana Cordeiro Cardoso,
  • Cláudia Moura Melo and
  • Paz M. S. Salazar-Schettino

Trypanosoma cruzi exhibits complex genetic diversity, organized into seven distinct typing units. To complete its life cycle, the parasite must adapt to the digestive tract of various species of triatomine bugs. This systematic review aimed to unders...

(This article belongs to the Special Issue Host–Microbe Interactions in Health and Disease)
  • Article
  • Open Access
976 Views
17 Pages

Impact of Pre-Existing Uterine Microbiome on Pregnancy Success After Embryo Transfer in Cattle

  • Nilton Luis Murga Valderrama,
  • Gleni T. Segura,
  • Jakson Ch Del Solar,
  • Hugo Frias,
  • Ana C. Romani,
  • Deiner J. Gongora-Bardales,
  • Ulises S. Quispe-Gutierrez,
  • Carla Maria Ordinola-Ramirez,
  • Richard C. Polveiro and
  • Rainer M. Lopez Lapa
  • + 2 authors

The uterine microbiome plays a critical role in maintaining pH balance, modulating the immune system, and influencing fertility, especially in artificial breeding contexts. This study examined the impact of uterine microbiota on pregnancy success in...

  • Article
  • Open Access
965 Views
12 Pages

Enterococcus durans Secretome Modulates Interleukins Gene Expressions in Intestinal Epithelial Cells Challenged by Staphylococcus aureus Secretome: In Vitro Study on the HT-29 Cell Line

  • Egidia Costanzi,
  • Giovanna Traina,
  • Marco Misuraca,
  • Donia Msakni,
  • Giada Sgaravizzi,
  • Musafiri Karama,
  • Ebtesam Al-Olayan,
  • Saeed El-Ashram,
  • Marcelo Martinez-Barbitta and
  • Beniamino T. Cenci-Goga
  • + 1 author

The present study examined the effect of Enterococcus durans cell-free supernatant (CFS) on interleukin (IL)-8, -10 and -1β gene expressions in the intestinal cell line HT-29 treated with Staphylococcus aureus CFS. HT-29 cells were incubated wit...

(This article belongs to the Special Issue Cell Culture Advances in Food Microbiology: Innovative In Vitro Models for Safety, Quality, and Functionality)
  • Article
  • Open Access
1 Citations
748 Views
13 Pages

Phytopythium species are water molds that have been divided as a separate group of oomycetes about 10 years ago. They are associated with diverse environments worldwide, but the ecological function of most of the species is still under investigation....

(This article belongs to the Special Issue Advances in Plant–Pathogen Interactions)
  • Communication
  • Open Access
993 Views
8 Pages

Serological Investigation of Infectious Bovine Rhinotracheitis in Dromedary Camels and Dairy Herds in Tunisia: Preliminary Results

  • Stefano Petrini,
  • Mohamed Methnani,
  • Cecilia Righi,
  • Khaled El Hicheri,
  • Cristina Casciari,
  • Aida Tatli,
  • Ben Smida Boubaker,
  • Elena Tinelli,
  • Sana Kacem and
  • Giovanni Pezzotti
  • + 3 authors

Livestock farming represents a key economic activity in the Tataouine Governorate of southern Tunisia, where cattle and dromedary camels coexist. Varicellovirus bovinealpha1 (BoAHV-1), the etiological agent of infectious bovine rhinotracheitis (IBR),...

(This article belongs to the Special Issue Emerging and Re-Emerging Viruses in Animal Health: Pathogenesis, Diagnosis, and One Health Strategies)
  • Review
  • Open Access
3 Citations
3,442 Views
28 Pages

Rapid Antimicrobial Susceptibility Testing (AST): Overview of New Commercially Available Automated Phenotypic Tools for Minimum Inhibitory Concentration (MIC) Determination

  • Giorgia Piccinini,
  • Antonio Curtoni,
  • Alessandro Bondi,
  • Mattia Genco,
  • Fabio Longo,
  • Carlotta Polizzi,
  • Paolo Valesella,
  • Silvia Corcione,
  • Francesco Giuseppe De Rosa and
  • Cristina Costa

Antimicrobial resistance (AMR) represents one of the most urgent global health threats, significantly impacting patient outcomes, healthcare systems, and economic sustainability. Rapid and accurate antimicrobial susceptibility testing (AST) are essen...

(This article belongs to the Special Issue Antimicrobial Resistance: From Surveillance to New Therapies)
  • Article
  • Open Access
980 Views
16 Pages

Molecular Epidemiology, Hematobiochemical Alterations, and Oxidative Stress-Induced Genotoxicity of Equine Trypanosomiasis in Pakistan

  • Waqas Ahmad,
  • Naeem Rasool,
  • Qurat ul Ain,
  • Usama Bin Naeem,
  • Muhammad Azeem,
  • Umbreen Anwar,
  • Tehreem Fayyaz,
  • Zeba Amjad,
  • Muhammad Yasin Tipu and
  • Mehmood Ahmad

Trypanosoma evansi (T. evansi) infection poses a significant health threat to equines. This study was aimed to assess the prevalence, risk factors, hematobiochemical alterations, and oxidative stress-mediated genotoxicity associated with equine trypa...

  • Article
  • Open Access
1,595 Views
12 Pages

Antimicrobial Effect of Slightly Acidic Hypochlorous Acid Water Against Biofilm Formed by Candida parapsilosis

  • Jun Iwahashi,
  • Akiko Shimizu,
  • Akinobu Togo,
  • Hiroshi Fuketa,
  • Kenji Gotoh,
  • Keisuke Ohta,
  • Norihiro Shinkai,
  • Naohisa Kawamura and
  • Hiroshi Watanabe

Background: Many of the pathogenic bacteria and fungi found in hospital environments form biofilms, which allow them to persist in the environment for long periods, posing a risk of hospital-acquired infections. Although the pathogens within biofilms...

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Microbiol. Res. - ISSN 2036-7481