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Open AccessArticle

Bafilomycin A1 and U18666A Efficiently Impair ZIKV Infection

1
Paul-Ehrlich-Institut, Department of Virology, 63225 Langen, Germany
2
German Center for Infection Research (DZIF), 38124 Braunschweig, Germany
*
Author to whom correspondence should be addressed.
Viruses 2019, 11(6), 524; https://doi.org/10.3390/v11060524
Received: 9 April 2019 / Revised: 24 May 2019 / Accepted: 4 June 2019 / Published: 6 June 2019
(This article belongs to the Section Antivirals & Vaccines)
Zika virus (ZIKV) is a highly transmissive virus that belongs to the Flaviviridae family, which comprises several other pathogens that threaten human health. This re-emerging virus gained attention during the outbreak in Brazil in 2016, where a considerable number of microcephaly cases in newborns was associated with ZIKV infection during pregnancy. Lacking a preventive vaccine or antiviral drugs, efforts have been made to better understand the viral life cycle. In light of this, the relevance of the endosomal–lysosomal compartment for the ZIKV life cycle was investigated. A549 and SH-SY5Y cells were infected with either the African strain (associated with mild symptoms) or the French Polynesia strain (associated with neurological complications). For both strains, the V-ATPase inhibitor, bafilomycin A1, efficiently inhibited ZIKV entry and prevented the spread of the infection by interfering with viral maturation. Additionally, affecting cholesterol metabolism and transport with the drug U18666A, which inactivates late endosomes and lysosomes, impairs the viral life cycle. The data presented show a clear antiviral effect of two compounds that target the same compartments in different ways. This highlights the relevance of the endosomal–lysosomal compartment for the viral life cycle that should be considered as a target for antivirals. View Full-Text
Keywords: Zika virus; viral life cycle; endosomal–lysosomal compartment; bafilomycin A1; U18666A; antiviral effect Zika virus; viral life cycle; endosomal–lysosomal compartment; bafilomycin A1; U18666A; antiviral effect
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Sabino, C.; Basic, M.; Bender, D.; Elgner, F.; Himmelsbach, K.; Hildt, E. Bafilomycin A1 and U18666A Efficiently Impair ZIKV Infection. Viruses 2019, 11, 524.

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