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Article

Isolation and Purification of a Neuroprotective Phlorotannin from the Marine Algae Ecklonia maxima by Size Exclusion and High-Speed Counter-Current Chromatography

Li Dak Sum Yip Yio Chin Kenneth Li Marine Biopharmaceutical Research Center, College of Food and Pharmaceutical Sciences, Ningbo University, Ningbo 315800, China
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Mar. Drugs 2019, 17(4), 212; https://doi.org/10.3390/md17040212
Received: 28 March 2019 / Revised: 1 April 2019 / Accepted: 3 April 2019 / Published: 4 April 2019
Phlorotannins are polyphenolic metabolites of marine brown algae that have been shown to possess health-beneficial biological activities. An efficient approach using a combination of high-speed counter-current chromatography (HSCCC) and size exclusion chromatography with a Sephadex LH-20 has been successfully developed for the isolation and purification of a neuroprotective phlorotannin, eckmaxol, from leaves of the marine brown algae, Ecklonia maxima. The phlorotannin of interest, eckmaxol, was isolated with purity >95% by HSCCC using an optimized solvent system composed of n-hexane–ethyl acetate–methanol–water (2:8:3:7, v/v/v/v) after Sephadex LH-20 size exclusion chromatography. This compound was successfully purified in the quantity of 5.2 mg from 0.3 kg of the E. maxima crude organic extract. The structure of eckmaxol was identified and assigned by NMR spectroscopic and mass spectrometric analyses. The purification method developed for eckmaxol will facilitate the further investigation and development of this neuroprotective agent as a drug lead or pharmacological probe. Furthermore, it is suggested that the combination of HSCCC and size exclusion chromatography could be more widely applied for the isolation and purification of phlorotannins from marine algae. View Full-Text
Keywords: phlorotannin; eckmaxol; high-speed counter-current chromatography; NMR spectroscopy; mass spectrometry; isolation and purification; Ecklonia maxima phlorotannin; eckmaxol; high-speed counter-current chromatography; NMR spectroscopy; mass spectrometry; isolation and purification; Ecklonia maxima
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MDPI and ACS Style

Zhou, X.; Yi, M.; Ding, L.; He, S.; Yan, X. Isolation and Purification of a Neuroprotective Phlorotannin from the Marine Algae Ecklonia maxima by Size Exclusion and High-Speed Counter-Current Chromatography. Mar. Drugs 2019, 17, 212. https://doi.org/10.3390/md17040212

AMA Style

Zhou X, Yi M, Ding L, He S, Yan X. Isolation and Purification of a Neuroprotective Phlorotannin from the Marine Algae Ecklonia maxima by Size Exclusion and High-Speed Counter-Current Chromatography. Marine Drugs. 2019; 17(4):212. https://doi.org/10.3390/md17040212

Chicago/Turabian Style

Zhou, Xuezhen, Mengqi Yi, Lijian Ding, Shan He, and Xiaojun Yan. 2019. "Isolation and Purification of a Neuroprotective Phlorotannin from the Marine Algae Ecklonia maxima by Size Exclusion and High-Speed Counter-Current Chromatography" Marine Drugs 17, no. 4: 212. https://doi.org/10.3390/md17040212

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