Elicited ROS Scavenging Activity, Photoprotective, and Wound-Healing Properties of Collagen-Derived Peptides from the Marine Sponge Chondrosia reniformis
Abstract
:1. Introduction
2. Results and Discussion
2.1. Marine Collagen Hydrolysate (MCHs) Purification Yield and Chemical Features
2.2. Effect of the MCP Fractions on Cell Growth of Specific Cell Lines
2.3. Radical Scavenging Activity of MCH Fractions
2.4. Effect of MCH Fractions on Fibroblast Collagen Expression and Release
2.5. Effect of MCP Fractions on UV-Induced Cell Death and Gene Expression
2.6. Effect of MCP Fractions on Wound Healing
3. Conclusions
4. Materials and Methods
4.1. Preparation of MCHs
4.2. SDS-Page Analysis
4.3. Degree of Hydrolysis (DH) Evaluation
4.4. HPLC MCP Purification
4.5. Hydroxyproline Content Evaluation
4.6. DPPH Radical Scavenging Activity
4.7. NBT Superoxide Anion Scavenging Activity
4.8. Cell Cultures
4.8.1. Cell Viability
4.8.2. ROS Detection in RAW 264.7 Cells
4.8.3. Collagen Quantification in the L929 Fibroblast Cell Medium
4.8.4. UV Treatment
4.8.5. Wound-Healing Assay
4.8.6. RNA Extraction, cDNA Synthesis, and qPCR Analyses
4.9. Statistical Analysis
Author Contributions
Funding
Conflicts of Interest
References
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µg/mL Hyp | |
---|---|
undigested CS | 83.21 ± 12.3 |
Total MCPs | 109.99 ± 4.78 |
M3 | 7.04 ± 0.32 |
M4 | 30.40 ± 6.72 |
M5 | 37.23 ± 3.56 |
M6 | 16.76 ± 4.51 |
P | Wound Healing Score in L929 Fibroblasts | |
---|---|---|
24 h | 30 h | |
CT | + | ++ |
M3 | ++ | ++ |
M4 | ++ | +++ |
M5 | +++ | +++ |
M6 | + | ++ |
GENE | GenBank | Forward | Reverse |
---|---|---|---|
Col1A MM | NM_007742.4 | 5′-CTgCTggTCCTgCTggTC-3′ | 5′-CCTTgTTCgCCTgTCTCAC-3′ |
Ubquitin MM | NM_019639 | 5′-GACAGGCAAGACCATCAC-3′ | 5′-TCTGAGGCGAAGGACTAAG-3′ |
Keratin 1 HS | NM_006121.3 | 5′AAgCCACACCACCATCAg-3′ | 5′CACCTCCAgAgCCATAgC-3′ |
Keratin 10 HS | NM_000421.3 | 5′-CCgAgTgCCAgAATACTgAATACC-3′ | 5′-TAgCCgCCgCCgAAACTTC-3′ |
Ubiquitin HS | NM_021009.6 | 5′-ATTTgggTCgCAgTTCTTg-3′ | 5′TgCCTTgACATTCTCgATggT-3′ |
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Pozzolini, M.; Millo, E.; Oliveri, C.; Mirata, S.; Salis, A.; Damonte, G.; Arkel, M.; Scarfì, S. Elicited ROS Scavenging Activity, Photoprotective, and Wound-Healing Properties of Collagen-Derived Peptides from the Marine Sponge Chondrosia reniformis. Mar. Drugs 2018, 16, 465. https://doi.org/10.3390/md16120465
Pozzolini M, Millo E, Oliveri C, Mirata S, Salis A, Damonte G, Arkel M, Scarfì S. Elicited ROS Scavenging Activity, Photoprotective, and Wound-Healing Properties of Collagen-Derived Peptides from the Marine Sponge Chondrosia reniformis. Marine Drugs. 2018; 16(12):465. https://doi.org/10.3390/md16120465
Chicago/Turabian StylePozzolini, Marina, Enrico Millo, Caterina Oliveri, Serena Mirata, Annalisa Salis, Gianluca Damonte, Maria Arkel, and Sonia Scarfì. 2018. "Elicited ROS Scavenging Activity, Photoprotective, and Wound-Healing Properties of Collagen-Derived Peptides from the Marine Sponge Chondrosia reniformis" Marine Drugs 16, no. 12: 465. https://doi.org/10.3390/md16120465