Sensors, Volume 26, Issue 10 (May-2 2026) – 361 articles
Pancreatic islets regulate blood glucose by secreting insulin, making glucose-stimulated insulin secretion the central functional readout for assessing islet function. However, conventional assays such as ELISA require offline processing and typically pool many islets, obscuring single-islet heterogeneity and rapid secretion dynamics. In this work, we present a microscopy-compatible islet-on-a-chip integrated with broadband backscattering confocal microscopy for continuous, label-free optical sensing of insulin secretion from individual human islets. Fabricated by two-photon polymerization, the device stabilizes single islets under continuous perfusion, while the optical readout identifies insulin granule-rich β-cells and tracks granule dynamics during glucose stimulation. View this paper
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