Characterization of ABC Transporters in EpiAirway™, a Cellular Model of Normal Human Bronchial Epithelium
Abstract
:1. Introduction
2. Results
3. Discussion
4. Material and Methods
4.1. Cell Cultures
4.2. Trans-epithelial Electrical Resistance (TEER) and Cellular Permeability
4.3. Bidirectional Transport Studies
4.4. Calculation of Papp
4.5. RT-qPCR Analysis
4.6. Western Blot Analysis
4.7. Immunocytochemistry and Immunohistochemistry
4.8. Statistical Analysis
4.9. Materials
Author Contributions
Funding
Conflicts of Interest
Abbreviations
BCRP | Breast Cancer Resistance Protein; |
HBSS | Hank’s Balanced Salt Solution; |
MDR1 | Multidrug Resistance 1; |
MRP1 | Multidrug Resistance-Associated Protein-1; |
Papp | apparent permeability coefficient; |
TEER | Transepithelial Electrical Resistance. |
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Rotoli, B.M.; Barilli, A.; Visigalli, R.; Ferrari, F.; Frati, C.; Lagrasta, C.A.; Di Lascia, M.; Riccardi, B.; Puccini, P.; Dall’Asta, V. Characterization of ABC Transporters in EpiAirway™, a Cellular Model of Normal Human Bronchial Epithelium. Int. J. Mol. Sci. 2020, 21, 3190. https://doi.org/10.3390/ijms21093190
Rotoli BM, Barilli A, Visigalli R, Ferrari F, Frati C, Lagrasta CA, Di Lascia M, Riccardi B, Puccini P, Dall’Asta V. Characterization of ABC Transporters in EpiAirway™, a Cellular Model of Normal Human Bronchial Epithelium. International Journal of Molecular Sciences. 2020; 21(9):3190. https://doi.org/10.3390/ijms21093190
Chicago/Turabian StyleRotoli, Bianca Maria, Amelia Barilli, Rossana Visigalli, Francesca Ferrari, Caterina Frati, Costanza Annamaria Lagrasta, Maria Di Lascia, Benedetta Riccardi, Paola Puccini, and Valeria Dall’Asta. 2020. "Characterization of ABC Transporters in EpiAirway™, a Cellular Model of Normal Human Bronchial Epithelium" International Journal of Molecular Sciences 21, no. 9: 3190. https://doi.org/10.3390/ijms21093190