Next Article in Journal
Affinity-Bead Assisted Mass Spectrometry (Affi-BAMS): A Multiplexed Microarray Platform for Targeted Proteomics
Next Article in Special Issue
Comparative Transcriptome Analysis Provides Insights into the Seed Germination in Cotton in Response to Chilling Stress
Previous Article in Journal
Role of microRNAs in the Development of Cardiovascular Disease in Systemic Autoimmune Disorders
Previous Article in Special Issue
Comprehensive Phosphoproteomic Analysis of Pepper Fruit Development Provides Insight into Plant Signaling Transduction
Article

A Silent Exonic Mutation in a Rice Integrin-α FG-GAP Repeat-Containing Gene Causes Male-Sterility by Affecting mRNA Splicing

1
State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu 611130, China
2
State Key Laboratory of Hybrid Rice, Rice Research Institute, Sichuan Agricultural University, Chengdu 611130, China
*
Authors to whom correspondence should be addressed.
These authors contributed equally to this work.
Int. J. Mol. Sci. 2020, 21(6), 2018; https://doi.org/10.3390/ijms21062018
Received: 12 February 2020 / Revised: 12 March 2020 / Accepted: 14 March 2020 / Published: 16 March 2020
(This article belongs to the Special Issue Plant Cell and Organism Development)
Pollen development plays crucial roles in the life cycle of higher plants. Here we characterized a rice mutant with complete male-sterile phenotype, pollen-less 1 (pl1). pl1 exhibited smaller anthers with arrested pollen development, absent Ubisch bodies, necrosis-like tapetal hypertrophy, and smooth anther cuticular surface. Molecular mapping revealed a synonymous mutation in the fourth exon of PL1 co-segregated with the mutant phenotype. This mutation disrupts the exon-intron splice junction in PL1, generating aberrant mRNA species and truncated proteins. PL1 is highly expressed in the tapetal cells of developing anther, and its protein is co-localized with plasma membrane (PM) and endoplasmic reticulum (ER) signal. PL1 encodes an integrin-α FG-GAP repeat-containing protein, which has seven β-sheets and putative Ca2+-binding motifs and is broadly conserved in terrestrial plants. Our findings therefore provide insights into both the role of integrin-α FG-GAP repeat-containing protein in rice male fertility and the influence of exonic mutation on intronic splice donor site selection. View Full-Text
Keywords: RNA splicing; PL1; rice (Oryza sativa L.); anther; pollen; male sterility; integrin-α FG-GAP repeat-containing protein RNA splicing; PL1; rice (Oryza sativa L.); anther; pollen; male sterility; integrin-α FG-GAP repeat-containing protein
Show Figures

Figure 1

MDPI and ACS Style

Zou, T.; Zhou, D.; Li, W.; Yuan, G.; Tao, Y.; He, Z.; Zhang, X.; Deng, Q.; Wang, S.; Zheng, A.; Zhu, J.; Liang, Y.; Liu, H.; Wang, A.; Wang, L.; Li, P.; Li, S. A Silent Exonic Mutation in a Rice Integrin-α FG-GAP Repeat-Containing Gene Causes Male-Sterility by Affecting mRNA Splicing. Int. J. Mol. Sci. 2020, 21, 2018. https://doi.org/10.3390/ijms21062018

AMA Style

Zou T, Zhou D, Li W, Yuan G, Tao Y, He Z, Zhang X, Deng Q, Wang S, Zheng A, Zhu J, Liang Y, Liu H, Wang A, Wang L, Li P, Li S. A Silent Exonic Mutation in a Rice Integrin-α FG-GAP Repeat-Containing Gene Causes Male-Sterility by Affecting mRNA Splicing. International Journal of Molecular Sciences. 2020; 21(6):2018. https://doi.org/10.3390/ijms21062018

Chicago/Turabian Style

Zou, Ting, Dan Zhou, Wenjie Li, Guoqiang Yuan, Yang Tao, Zhiyuan He, Xu Zhang, Qiming Deng, Shiquan Wang, Aiping Zheng, Jun Zhu, Yueyang Liang, Huainian Liu, Aijun Wang, Lingxia Wang, Ping Li, and Shuangcheng Li. 2020. "A Silent Exonic Mutation in a Rice Integrin-α FG-GAP Repeat-Containing Gene Causes Male-Sterility by Affecting mRNA Splicing" International Journal of Molecular Sciences 21, no. 6: 2018. https://doi.org/10.3390/ijms21062018

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop