Open AccessArticle
A Silent Exonic Mutation in a Rice Integrin-α FG-GAP Repeat-Containing Gene Causes Male-Sterility by Affecting mRNA Splicing
by
Ting Zou, Dan Zhou, Wenjie Li, Guoqiang Yuan, Yang Tao, Zhiyuan He, Xu Zhang, Qiming Deng, Shiquan Wang, Aiping Zheng, Jun Zhu, Yueyang Liang, Huainian Liu, Aijun Wang, Lingxia Wang, Ping Li and Shuangcheng Li
Cited by 6 | Viewed by 4441
Abstract
Pollen development plays crucial roles in the life cycle of higher plants. Here we characterized a rice mutant with complete male-sterile phenotype,
pollen-less 1 (
pl1).
pl1 exhibited smaller anthers with arrested pollen development, absent Ubisch bodies, necrosis-like tapetal hypertrophy, and smooth
[...] Read more.
Pollen development plays crucial roles in the life cycle of higher plants. Here we characterized a rice mutant with complete male-sterile phenotype,
pollen-less 1 (
pl1).
pl1 exhibited smaller anthers with arrested pollen development, absent Ubisch bodies, necrosis-like tapetal hypertrophy, and smooth anther cuticular surface. Molecular mapping revealed a synonymous mutation in the fourth exon of
PL1 co-segregated with the mutant phenotype. This mutation disrupts the exon-intron splice junction in
PL1, generating aberrant mRNA species and truncated proteins.
PL1 is highly expressed in the tapetal cells of developing anther, and its protein is co-localized with plasma membrane (PM) and endoplasmic reticulum (ER) signal.
PL1 encodes an integrin-α FG-GAP repeat-containing protein, which has seven β-sheets and putative Ca
2+-binding motifs and is broadly conserved in terrestrial plants. Our findings therefore provide insights into both the role of integrin-α FG-GAP repeat-containing protein in rice male fertility and the influence of exonic mutation on intronic splice donor site selection.
Full article
►▼
Show Figures
