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Article

EMT Transcription Factors Are Involved in the Altered Cell Adhesion under Simulated Microgravity Effect or Overloading by Regulation of E-cadherin

1
School of Life Science and Technology, Harbin Institute of Technology, Harbin 150001, China
2
College of Life Science, China West Normal University, Nanchong 637002, China
3
State Key Laboratory of Space Medicine Fundamentals and Application, Chinese Astronaut Research and Training Center, Beijing 100094, China
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2020, 21(4), 1349; https://doi.org/10.3390/ijms21041349
Received: 31 December 2019 / Revised: 29 January 2020 / Accepted: 12 February 2020 / Published: 17 February 2020
(This article belongs to the Special Issue Microgravity and Cell Adherence)
In order to study the effect of stress changes on cell adhesion, HUVEC, and MCF-7 cells were treated with simulated microgravity effect (SMG) and overloading (OL). Methods: Rotating Wall Vessel (2D-RWVS) bioreactor was used to create different culture conditions. In addition, the alteration of cell adhesion states, adhesion proteins, and relating factors of adhesion molecules under these two conditions were detected using cell adhesion assay, immunofluorescence, western blot, and qRT-PCR technology. Results: The results showed that the adhesion of cells decreased under SMG, while increased under OL. The expressions of integrin β1, paxillin, and E-cadherin under SMG condition were down-regulated as compared to that of the control group showing a time-dependent pattern of the decreasing. However, under OL condition, the expressions of adhesion proteins were up-regulated as compared to that of the control group, with a time-dependent pattern of increasing. EMT transcription factors Snail, twist, and ZEB1 were up-regulated under SMG while down-regulated under OL. Conclusion: Collectively our results indicated that cells could respond to stress changes to regulate the expressions of adhesion proteins and adapt their adhesion state to the altered mechanical environment. The altered cell adhesion in response to the mechanical stress may involve the changed expression of EMT-inducing factors, Snail, Twist, and ZEB1under the SMG/OL conditions. View Full-Text
Keywords: simulated microgravity effect (SMG); mechanical overloading (OL); cell adhesion; HUVEC and MCF-7 cell simulated microgravity effect (SMG); mechanical overloading (OL); cell adhesion; HUVEC and MCF-7 cell
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MDPI and ACS Style

Shi, S.; Li, Q.; Cao, Q.; Diao, Y.; Zhang, Y.; Yue, L.; Wei, L. EMT Transcription Factors Are Involved in the Altered Cell Adhesion under Simulated Microgravity Effect or Overloading by Regulation of E-cadherin. Int. J. Mol. Sci. 2020, 21, 1349. https://doi.org/10.3390/ijms21041349

AMA Style

Shi S, Li Q, Cao Q, Diao Y, Zhang Y, Yue L, Wei L. EMT Transcription Factors Are Involved in the Altered Cell Adhesion under Simulated Microgravity Effect or Overloading by Regulation of E-cadherin. International Journal of Molecular Sciences. 2020; 21(4):1349. https://doi.org/10.3390/ijms21041349

Chicago/Turabian Style

Shi, Shuliang, Qiao Li, Qiuying Cao, Yan Diao, Yao Zhang, Lei Yue, and Lijun Wei. 2020. "EMT Transcription Factors Are Involved in the Altered Cell Adhesion under Simulated Microgravity Effect or Overloading by Regulation of E-cadherin" International Journal of Molecular Sciences 21, no. 4: 1349. https://doi.org/10.3390/ijms21041349

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