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Open AccessArticle

Assessment of a 18F-Phenylboronic Acid Radiotracer for Imaging Boron in Maize

1
Missouri Research Reactor Center, University of Missouri, Columbia, MO 65211, USA
2
Chemistry Department, University of Missouri, Columbia, MO 65211, USA
3
Division of Biological Sciences, Interdisciplinary Plant Group, University of Missouri, Columbia, MO 65211, USA
4
Missouri Maize Center, University of Missouri, Columbia, MO 65211, USA
5
Department of Veterinary Medicine and Surgery, Columbia, MO 65211, USA
6
Bond Life Sciences Center, University of Missouri, Columbia, MO 65211, USA
7
Division of Plant Sciences, Interdisciplinary Plant Group, University of Missouri, Columbia, MO 65211, USA
*
Author to whom correspondence should be addressed.
These authors contributed equally to this paper.
Int. J. Mol. Sci. 2020, 21(3), 976; https://doi.org/10.3390/ijms21030976
Received: 31 December 2019 / Accepted: 29 January 2020 / Published: 1 February 2020
(This article belongs to the Special Issue Novel Aspects of Boron Biology in Plants. Boron and Plant Interaction)
Boron (B) is an essential plant micronutrient. Deficiencies of B have drastic consequences on plant development leading to crop yield losses and reductions in root and shoot growth. Understanding the molecular and cellular consequences of B deficiency is challenging, partly because of the limited availability of B imaging techniques. In this report we demonstrate the efficacy of using 4-fluorophenylboronic acid (FPBA) as a B imaging agent, which is a derivative of the B deficiency mimic phenylboronic acid (PBA). We show that radioactively labelled [18F]FPBA (t½=110 m) accumulates at the root tip, the root elongation zone and at lateral root initiation sites in maize roots, and also translocates to the shoot where it accumulates along the leaf edges. Treatment of maize seedlings using FPBA and PBA causes a shortened primary root phenotype with absence of lateral roots in a dose-dependent manner. The primary root defects can be partially rescued by the addition of boric acid indicating that PBA can be used to induce B deficiency in maize and that radioactively labelled FPBA can be used to image sites of B demand on a tissue level. View Full-Text
Keywords: boron; phenylboronic acid; maize; radiotracer boron; phenylboronic acid; maize; radiotracer
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Housh, A.B.; Matthes, M.S.; Gerheart, A.; Wilder, S.L.; Kil, K.-E.; Schueller, M.; Guthrie, J.M.; McSteen, P.; Ferrieri, R. Assessment of a 18F-Phenylboronic Acid Radiotracer for Imaging Boron in Maize. Int. J. Mol. Sci. 2020, 21, 976.

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