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Article

The Impact of Human Lipoaspirate and Adipose Tissue-Derived Stem Cells Contact Culture on Breast Cancer Cells: Implications in Breast Reconstruction

Department of Plastic Surgery, University of Pittsburgh, Pittsburgh, PA 15261, USA
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Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2020, 21(23), 9171; https://doi.org/10.3390/ijms21239171
Received: 27 August 2020 / Revised: 19 November 2020 / Accepted: 27 November 2020 / Published: 1 December 2020
Background: Autologous fat transfer in the form of lipoaspirates for the reconstruction of the breast after breast cancer surgery is a commonly used procedure in plastic surgery. However, concerns regarding the oncologic risk of nutrient-rich fat tissue are widely debated. Previous studies have primarily focused on studying the interaction between adipose-derived stem cells (ASCs) and breast cancer cells. Methods: In this study, we performed a comprehensive analysis of the paracrine- and contact-based interactions between lipoaspirates, ASCs and breast cancer cell lines. An inverted flask culture method was used to study the contact-based interaction between lipoaspirates and breast cancer cells, while GFP-expressing breast cancer cell lines were generated to study the cell–cell contact interaction with ASCs. Three different human breast cancer cell lines, MCF-7, MDA-MB-231 and BT-474, were studied. We analyzed the impact of these interactions on the proliferation, cell cycle and epithelial-to-mesenchymal (EMT) transition of the breast cancer cells. Results: Our results revealed that both lipoaspirates and ASCs do not increase the proliferation rate of the breast cancer cells either through paracrine- or contact-dependent interactions. We observed that lipoaspirates selectively inhibit the proliferation of MCF-7 cells in contact co-culture, driven by the retinoblastoma (Rb) protein activity mediating cell cycle arrest. Additionally, ASCs inhibited MDA-MB-231 breast cancer cell proliferation in cell–cell contact-dependent interactions. Quantitative real-time PCR revealed no significant increase in the EMT-related genes in breast cancer cells upon co-culture with ASCs. Conclusion: In conclusion, this study provides evidence of the non-oncogenic character of lipoaspirates and supports the safety of clinical fat grafting in breast reconstruction after oncological surgical procedures. In vivo studies in appropriate animal models and long-term post-operative clinical data from patients are essential to reach the final safety recommendations. View Full-Text
Keywords: adipose-derived stem cells; lipoaspirates; breast cancer cells; cell cycle and proliferation adipose-derived stem cells; lipoaspirates; breast cancer cells; cell cycle and proliferation
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MDPI and ACS Style

Ejaz, A.; Yang, K.S.; Venkatesh, K.P.; Chinnapaka, S.; Kokai, L.E.; Rubin, J.P. The Impact of Human Lipoaspirate and Adipose Tissue-Derived Stem Cells Contact Culture on Breast Cancer Cells: Implications in Breast Reconstruction. Int. J. Mol. Sci. 2020, 21, 9171. https://doi.org/10.3390/ijms21239171

AMA Style

Ejaz A, Yang KS, Venkatesh KP, Chinnapaka S, Kokai LE, Rubin JP. The Impact of Human Lipoaspirate and Adipose Tissue-Derived Stem Cells Contact Culture on Breast Cancer Cells: Implications in Breast Reconstruction. International Journal of Molecular Sciences. 2020; 21(23):9171. https://doi.org/10.3390/ijms21239171

Chicago/Turabian Style

Ejaz, Asim, Katherine S. Yang, Kaushik P. Venkatesh, Somaiah Chinnapaka, Lauren E. Kokai, and J. P. Rubin. 2020. "The Impact of Human Lipoaspirate and Adipose Tissue-Derived Stem Cells Contact Culture on Breast Cancer Cells: Implications in Breast Reconstruction" International Journal of Molecular Sciences 21, no. 23: 9171. https://doi.org/10.3390/ijms21239171

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