Tricarboxylic Acid Cycle Metabolites as Mediators of DNA Methylation Reprogramming in Bovine Preimplantation Embryos
Abstract
:1. Introduction
2. Results
2.1. DNA Methylation Reprogramming Failure in Slow Developing Embryos
2.2. Metabolic Modulation Is Involved with Epigenetic Reprogramming
3. Discussion
4. Materials and Methods
4.1. Chemicals and Reagents
4.2. Experimental Design
4.3. In Vitro Embryo Production (IVP)
4.4. Immunofluorescence for 5mC and 5hmC
4.5. RNA-Seq Analysis
4.6. RNA-Seq Validation
4.7. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Ispada, J.; da Fonseca Junior, A.M.; de Lima, C.B.; dos Santos, E.C.; Fontes, P.K.; Nogueira, M.F.G.; da Silva, V.L.; Almeida, F.N.; Leite, S.d.C.; Chitwood, J.L.; et al. Tricarboxylic Acid Cycle Metabolites as Mediators of DNA Methylation Reprogramming in Bovine Preimplantation Embryos. Int. J. Mol. Sci. 2020, 21, 6868. https://doi.org/10.3390/ijms21186868
Ispada J, da Fonseca Junior AM, de Lima CB, dos Santos EC, Fontes PK, Nogueira MFG, da Silva VL, Almeida FN, Leite SdC, Chitwood JL, et al. Tricarboxylic Acid Cycle Metabolites as Mediators of DNA Methylation Reprogramming in Bovine Preimplantation Embryos. International Journal of Molecular Sciences. 2020; 21(18):6868. https://doi.org/10.3390/ijms21186868
Chicago/Turabian StyleIspada, Jessica, Aldcejam Martins da Fonseca Junior, Camila Bruna de Lima, Erika Cristina dos Santos, Patricia Kubo Fontes, Marcelo Fábio Gouveia Nogueira, Vinicius Lourenço da Silva, Fernanda Nascimento Almeida, Saul de Castro Leite, James Lee Chitwood, and et al. 2020. "Tricarboxylic Acid Cycle Metabolites as Mediators of DNA Methylation Reprogramming in Bovine Preimplantation Embryos" International Journal of Molecular Sciences 21, no. 18: 6868. https://doi.org/10.3390/ijms21186868