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Open AccessArticle

Transcriptome Profiling Reveals Novel Candidate Genes Related to Hippocampal Dysfunction in SREBP-1c Knockout Mice

1
Department of Veterinary Anatomy, College of Veterinary Medicine and BK21 Plus Project Team, Chonnam National University, Gwangju 61186, Korea
2
Herbal Medicine Resources Research Center, Korea Institute of Oriental Medicine, 111 Geonjae-ro, Naju-si 58245, Korea
3
Department of Animal Science, College of Agriculture and Life Science, Chonnam National University, Gwangju 61186, Korea
4
Department of Physiology, Keimyung University School of Medicine, Daegu 42601, Korea
*
Author to whom correspondence should be addressed.
First three authors (M.J.A., J.K. and S.L.) contributed equally to this work.
Int. J. Mol. Sci. 2020, 21(11), 4131; https://doi.org/10.3390/ijms21114131
Received: 3 May 2020 / Revised: 29 May 2020 / Accepted: 8 June 2020 / Published: 10 June 2020
Lipid homeostasis is an important component of brain function, and its disturbance causes several neurological disorders, such as Huntington’s, Alzheimer’s, and Parkinson’s diseases as well as mood disorders. Sterol regulatory element-binding protein-1c (SREBP-1c) is a key modulatory molecule involved in lipid homeostasis in the central nervous system. However, little is known about the biological effects of SREBP-1c in the brain. Our previous study uncovered that mice deficient in SREBP-1c exhibit schizophrenia-like behaviors. To investigate whether there are novel molecular mechanisms involved in the neurological aberrations caused by SREBP-1c deficiency, we analyzed the transcriptomes of the hippocampus of SREBP-1c knockout (KO) mice and wild-type mice. We found seven differentially expressed genes (three up-regulated and four down-regulated genes) in the hippocampus of SREBP-1c KO mice. For further verification, we selected the three most significantly changed genes: glucagon-like peptide 2 receptors (GLP2R) involved in hippocampal neurogenesis and neuroplasticity as well as in cognitive impairments; necdin (NDN) which is related to neuronal death and neurodevelopmental disorders; and Erb-B2 receptor tyrosine kinase 4 (ERBB4) which is a receptor for schizophrenia-linked protein, neuregulin-1. The protein levels of GLP2R and NDN were considerably decreased, but the level of ERBB4 was significantly increased in the hippocampus of SREBP-1c KO mice. However, further confirmation is warranted to establish the translatability of these findings from this rodent model into human patients. We suggest that these data provide novel molecular evidence for the modulatory role of SREBP-1c in the mouse hippocampus. View Full-Text
Keywords: SREBP-1c; RNA-sequencing; hippocampus; lipid metabolism; brain; mouse model; schizophrenia; transcriptome profiling; novel genes SREBP-1c; RNA-sequencing; hippocampus; lipid metabolism; brain; mouse model; schizophrenia; transcriptome profiling; novel genes
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MDPI and ACS Style

Ang, M.J.; Kim, J.; Lee, S.; Kim, S.-H.; Kim, J.-C.; Jeon, T.-I.; Im, S.-S.; Moon, C. Transcriptome Profiling Reveals Novel Candidate Genes Related to Hippocampal Dysfunction in SREBP-1c Knockout Mice. Int. J. Mol. Sci. 2020, 21, 4131. https://doi.org/10.3390/ijms21114131

AMA Style

Ang MJ, Kim J, Lee S, Kim S-H, Kim J-C, Jeon T-I, Im S-S, Moon C. Transcriptome Profiling Reveals Novel Candidate Genes Related to Hippocampal Dysfunction in SREBP-1c Knockout Mice. International Journal of Molecular Sciences. 2020; 21(11):4131. https://doi.org/10.3390/ijms21114131

Chicago/Turabian Style

Ang, Mary J.; Kim, Juhwan; Lee, Sueun; Kim, Sung-Ho; Kim, Jong-Choon; Jeon, Tae-Il; Im, Seung-Soon; Moon, Changjong. 2020. "Transcriptome Profiling Reveals Novel Candidate Genes Related to Hippocampal Dysfunction in SREBP-1c Knockout Mice" Int. J. Mol. Sci. 21, no. 11: 4131. https://doi.org/10.3390/ijms21114131

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