Insights into the Mn2+ Binding Site in the Agmatinase-Like Protein (ALP): A Critical Enzyme for the Regulation of Agmatine Levels in Mammals
Abstract
:1. Introduction
2. Results and Discussion
2.1. Manganese Binding Site in ALP
2.2. Expression and Characterization of Central-ALP
2.3. Site-Directed Mutagenesis of Putative Mn2+ Ligands
3. Conclusions
4. Materials and Methods
4.1. Materials
4.2. Molecular Modeling
4.3. Enzyme Preparations
4.4. ALP Activity Determination
4.5. Enzyme–Metal Interactions Analysis
4.6. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Km (mM) | kcat (s−¹) | Kact Mn2+ (M) | |
---|---|---|---|
ALP-wt | 3.0 ± 0.20 | 0.9 ± 0.2 | 1.88 × 10−8 |
∆LIM-ALP | 1.2 ± 0.04 | 10 ± 1 | 3.6 × 10−8 |
∆LIM-ALP/D217A | No activity | No activity | No activity |
∆LIM-ALP/E287A/K289A | No activity | No activity | No activity |
∆LIM-ALP/N213A/Q215A/D217A | No activity | No activity | No activity |
Central-ALP | 1.2 ± 0.37 | 0.4 ± 0.5 | 1.7 × 10−8 |
Central-ALP (65 °C) | 1.2 ± 0.37 | 0.8 ± 0.4 | 2.2 × 10−8 |
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Reyes, M.-B.; Martínez-Oyanedel, J.; Navarrete, C.; Mardones, E.; Martínez, I.; Salas, M.; López, V.; García-Robles, M.; Tarifeño-Saldivia, E.; Figueroa, M.; et al. Insights into the Mn2+ Binding Site in the Agmatinase-Like Protein (ALP): A Critical Enzyme for the Regulation of Agmatine Levels in Mammals. Int. J. Mol. Sci. 2020, 21, 4132. https://doi.org/10.3390/ijms21114132
Reyes M-B, Martínez-Oyanedel J, Navarrete C, Mardones E, Martínez I, Salas M, López V, García-Robles M, Tarifeño-Saldivia E, Figueroa M, et al. Insights into the Mn2+ Binding Site in the Agmatinase-Like Protein (ALP): A Critical Enzyme for the Regulation of Agmatine Levels in Mammals. International Journal of Molecular Sciences. 2020; 21(11):4132. https://doi.org/10.3390/ijms21114132
Chicago/Turabian StyleReyes, María-Belen, José Martínez-Oyanedel, Camila Navarrete, Erika Mardones, Ignacio Martínez, Mónica Salas, Vasthi López, María García-Robles, Estefania Tarifeño-Saldivia, Maximiliano Figueroa, and et al. 2020. "Insights into the Mn2+ Binding Site in the Agmatinase-Like Protein (ALP): A Critical Enzyme for the Regulation of Agmatine Levels in Mammals" International Journal of Molecular Sciences 21, no. 11: 4132. https://doi.org/10.3390/ijms21114132