Next Article in Journal
Amino Acid Transport Defects in Human Inherited Metabolic Disorders
Previous Article in Journal
Characterization of the Inhibitory Effect of Gastrodigenin and Gastrodin on M-type K+ Currents in Pituitary Cells and Hippocampal Neurons
 
 
Article

Comparative Proteomic Analysis Reveals the Regulatory Effects of H2S on Salt Tolerance of Mangrove Plant Kandelia obovata

Key Laboratory of the Ministry of Education for Coastal and Wetland Ecosystems, College of the Environment and Ecology, Xiamen University, Xiamen 361102, China
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2020, 21(1), 118; https://doi.org/10.3390/ijms21010118
Received: 22 November 2019 / Revised: 17 December 2019 / Accepted: 19 December 2019 / Published: 23 December 2019
(This article belongs to the Section Molecular Biology)
As a dominant mangrove species, Kandelia obovata is distributed in an intertidal marsh with an active H2S release. Whether H2S participates in the salt tolerance of mangrove plants is still ambiguous, although increasing evidence has demonstrated that H2S functions in plant responses to multiple abiotic stresses. In this study, NaHS was used as an H2S donor to investigate the regulatory mechanism of H2S on the salt tolerance of K. obovata seedlings by using a combined physiological and proteomic analysis. The results showed that the reduction in photosynthesis (Pn) caused by 400 mM of NaCl was recovered by the addition of NaHS (200 μM). Furthermore, the application of H2S enhanced the quantum efficiency of photosystem II (PSII) and the membrane lipid stability, implying that H2S is beneficial to the survival of K. obovata seedlings under high salinity. We further identified 37 differentially expressed proteins by proteomic approaches under salinity and NaHS treatments. Among them, the proteins that are related to photosynthesis, primary metabolism, stress response and hormone biosynthesis were primarily enriched. The physiological and proteomic results highlighted that exogenous H2S up-regulated photosynthesis and energy metabolism to help K. obovata to cope with high salinity. Specifically, H2S increased photosynthetic electron transfer, chlorophyll biosynthesis and carbon fixation in K. obovata leaves under salt stress. Furthermore, the abundances of other proteins related to the metabolic pathway, such as antioxidation (ascorbic acid peroxidase (APX), copper/zinc superoxide dismutase (CSD2), and pancreatic and duodenal homeobox 1 (PDX1)), protein synthesis (heat-shock protein (HSP), chaperonin family protein (Cpn) 20), nitrogen metabolism (glutamine synthetase 1 and 2 (GS2), GS1:1), glycolysis (phosphoglycerate kinase (PGK) and triosephosphate isomerase (TPI)), and the ascorbate–glutathione (AsA–GSH) cycle were increased by H2S under high salinity. These findings provide new insights into the roles of H2S in the adaptations of the K. obovata mangrove plant to high salinity environments. View Full-Text
Keywords: Kandelia obovata; mangrove; hydrogen sulfide; salt tolerance; comparative proteome Kandelia obovata; mangrove; hydrogen sulfide; salt tolerance; comparative proteome
Show Figures

Graphical abstract

MDPI and ACS Style

Liu, Y.-L.; Shen, Z.-J.; Simon, M.; Li, H.; Ma, D.-N.; Zhu, X.-Y.; Zheng, H.-L. Comparative Proteomic Analysis Reveals the Regulatory Effects of H2S on Salt Tolerance of Mangrove Plant Kandelia obovata. Int. J. Mol. Sci. 2020, 21, 118. https://doi.org/10.3390/ijms21010118

AMA Style

Liu Y-L, Shen Z-J, Simon M, Li H, Ma D-N, Zhu X-Y, Zheng H-L. Comparative Proteomic Analysis Reveals the Regulatory Effects of H2S on Salt Tolerance of Mangrove Plant Kandelia obovata. International Journal of Molecular Sciences. 2020; 21(1):118. https://doi.org/10.3390/ijms21010118

Chicago/Turabian Style

Liu, Yi-Ling, Zhi-Jun Shen, Martin Simon, Huan Li, Dong-Na Ma, Xue-Yi Zhu, and Hai-Lei Zheng. 2020. "Comparative Proteomic Analysis Reveals the Regulatory Effects of H2S on Salt Tolerance of Mangrove Plant Kandelia obovata" International Journal of Molecular Sciences 21, no. 1: 118. https://doi.org/10.3390/ijms21010118

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop