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Characterization of the Inhibitory Effect of Gastrodigenin and Gastrodin on M-type K+ Currents in Pituitary Cells and Hippocampal Neurons

1
Department of Neurology, Taichung Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation, Taichung City 42743, Taiwan
2
Department of Pediatrics, Chi-Mei Medical Center, Tainan City 71004, Taiwan
3
Department of Cardiology, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, Tainan City 70101, Taiwan
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Department of Pharmacology, College of Medicine, Kaohsiung Medical University, Kaohsiung City 80708, Taiwan
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Department of Neurology, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, Tainan City 70101, Taiwan
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Department of Physiology, National Cheng Kung University Medical College, Tainan City 70101, Taiwan
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Institute of Basic Medical Sciences, National Cheng Kung University Medical College, Tainan City 70101, Taiwan
8
Department of Medical Research, China Medical University Hospital, China Medical University, Taichung 40402, Taiwan
*
Authors to whom correspondence should be addressed.
Int. J. Mol. Sci. 2020, 21(1), 117; https://doi.org/10.3390/ijms21010117
Received: 25 November 2019 / Revised: 15 December 2019 / Accepted: 20 December 2019 / Published: 23 December 2019
(This article belongs to the Section Bioactives and Nutraceuticals)
Gastrodigenin (HBA) and gastrodin (GAS) are phenolic ingredients found in Gastrodia elata Blume (GEB), a traditional Chinese herbal medicine. These compounds have been previously used to treat cognitive dysfunction, convulsion, and dizziness. However, at present, there is no available information regarding their potential ionic effects in electrically excitable cells. In the current study, the possible effects of HBA and GAS on different ionic currents in pituitary GH3 cells and hippocampal mHippoE-14 neurons were investigated using the patch-clamp technique. The addition of HBA or GAS resulted in the differential inhibition of the M-type K+ current (IK(M)) density in a concentration-dependent manner in GH3 cells. HBA resulted in a slowing of the activation time course of IK(M), while GAS elevated it. HBA also mildly suppressed the density of erg-mediated or the delayed-rectifier K+ current in GH3 cells. Neither GAS nor HBA (10 µM) modified the voltage-gated Na+ current density, although they suppressed the L-type Ca2+ current density at the same concentration. In hippocampal mHippoE-14 neurons, HBA was effective at inhibiting IK(M) density as well as slowing the activation time course. Taken together, the present study provided the first evidence that HBA or GAS could act on cellular mechanisms, and could therefore potentially have a functional influence in various neurologic disorders. View Full-Text
Keywords: gastrodigenin; p-hydroxybenzyl alcohol; gastrodin; M-type K+ current; L-type Ca2+ current and voltage-gated Na+ current gastrodigenin; p-hydroxybenzyl alcohol; gastrodin; M-type K+ current; L-type Ca2+ current and voltage-gated Na+ current
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MDPI and ACS Style

Yang, C.-S.; Lai, M.-C.; Liu, P.-Y.; Lo, Y.-C.; Huang, C.-W.; Wu, S.-N. Characterization of the Inhibitory Effect of Gastrodigenin and Gastrodin on M-type K+ Currents in Pituitary Cells and Hippocampal Neurons. Int. J. Mol. Sci. 2020, 21, 117. https://doi.org/10.3390/ijms21010117

AMA Style

Yang C-S, Lai M-C, Liu P-Y, Lo Y-C, Huang C-W, Wu S-N. Characterization of the Inhibitory Effect of Gastrodigenin and Gastrodin on M-type K+ Currents in Pituitary Cells and Hippocampal Neurons. International Journal of Molecular Sciences. 2020; 21(1):117. https://doi.org/10.3390/ijms21010117

Chicago/Turabian Style

Yang, Chih-Sheng, Ming-Chi Lai, Ping-Yen Liu, Yi-Ching Lo, Chin-Wei Huang, and Sheng-Nan Wu. 2020. "Characterization of the Inhibitory Effect of Gastrodigenin and Gastrodin on M-type K+ Currents in Pituitary Cells and Hippocampal Neurons" International Journal of Molecular Sciences 21, no. 1: 117. https://doi.org/10.3390/ijms21010117

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