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Int. J. Mol. Sci. 2018, 19(9), 2723; https://doi.org/10.3390/ijms19092723

Chaperone-E3 Ligase Complex HSP70-CHIP Mediates Ubiquitination of Ribosomal Protein S3

1
Department of Molecular Cell Biology, Sungkyunkwan University School of Medicine, Suwon 16419, Korea
2
Single Cell Network Research Center, Sungkyunkwan University School of Medicine, Suwon 16419, Korea
3
Department of Biochemistry and Molecular Biology, University of Ulsan, College of Medicine, Seoul 05505, Korea
4
Samsung Biomedical Research Institute, Samsung Medical Center, Seoul 06351, Korea
*
Author to whom correspondence should be addressed.
Received: 3 July 2018 / Revised: 7 September 2018 / Accepted: 10 September 2018 / Published: 12 September 2018
(This article belongs to the Special Issue Neuron Cell Death)
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Abstract

In addition to its role in ribosome biogenesis, ribosomal protein S3 (RPS3), a component of the 40S ribosomal subunit, has been suggested to possess several extraribosomal functions, including an apoptotic function. In this study, we demonstrated that in the mouse brain, the protein levels of RPS3 were altered by the degree of nutritional starvation and correlated with neuronal apoptosis. After endurable short-term starvation, the apoptotic function of RPS3 was suppressed by Akt activation and Akt-mediated T70 phosphorylation, whereas after prolonged starvation, the protein levels of RPS3 notably increased, and abundant neuronal death occurred. These events coincided with ubiquitination and subsequent degradation of RPS3, controlled by HSP70 and the cochaperone E3 ligase: carboxy terminus of heat shock protein 70-interacting protein (CHIP). Thus, our study points to an extraribosomal role of RPS3 in balancing neuronal survival or death depending on the degree of starvation through CHIP-mediated polyubiquitination and degradation. View Full-Text
Keywords: neuronal apoptosis; ribosomal protein S3 (RPS3); Akt/PKB (protein kinase B); carboxy terminus of heat shock protein 70-interacting protein (CHIP) neuronal apoptosis; ribosomal protein S3 (RPS3); Akt/PKB (protein kinase B); carboxy terminus of heat shock protein 70-interacting protein (CHIP)
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Hwang, I.; Cho, S.-W.; Ahn, J.-Y. Chaperone-E3 Ligase Complex HSP70-CHIP Mediates Ubiquitination of Ribosomal Protein S3. Int. J. Mol. Sci. 2018, 19, 2723.

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