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Search Results (9,789)

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Keywords = yield sustainability

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11 pages, 638 KiB  
Communication
Millet in Bioregenerative Life Support Systems: Hypergravity Resilience and Predictive Yield Models
by Tatiana S. Aniskina, Arkady N. Kudritsky, Olga A. Shchuklina, Nikita E. Andreev and Ekaterina N. Baranova
Life 2025, 15(8), 1261; https://doi.org/10.3390/life15081261 (registering DOI) - 7 Aug 2025
Abstract
The prospects for long-distance space flights are becoming increasingly realistic, and one of the key factors for their implementation is the creation of sustainable systems for producing food on site. Therefore, the aim of our work is to assess the prospects for using [...] Read more.
The prospects for long-distance space flights are becoming increasingly realistic, and one of the key factors for their implementation is the creation of sustainable systems for producing food on site. Therefore, the aim of our work is to assess the prospects for using millet in biological life support systems and to create predictive models of yield components for automating plant cultivation control. The study found that stress from hypergravity (800 g, 1200 g, 2000 g, and 3000 g) in the early stages of millet germination does not affect seedlings or yield. In a closed system, millet yield reached 0.31 kg/m2, the weight of 1000 seeds was 8.61 g, and the yield index was 0.06. The paper describes 40 quantitative traits, including six leaf and trichome traits and nine grain traits from the lower, middle and upper parts of the inflorescence. The compiled predictive regression equations allow predicting the accumulation of biomass in seedlings on the 10th and 20th days of cultivation, as well as the weight of 1000 seeds, the number of productive inflorescences, the total above-ground mass, and the number and weight of grains per plant. These equations open up opportunities for the development of computer vision and high-speed plant phenotyping programs that will allow automatic correction of the plant cultivation process and modeling of the required yield. Predicting biomass yield will also be useful in assessing the load on the waste-free processing system for plant waste at planetary stations. Full article
(This article belongs to the Special Issue Physiological Responses of Plants Under Abiotic Stresses)
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14 pages, 706 KiB  
Article
Study on the Effects of Irrigation Amount on Spring Maize Yield and Water Use Efficiency Under Different Planting Patterns in Xinjiang
by Ruxiao Bai, Haixiu He, Xinjiang Zhang and Qifeng Wu
Agriculture 2025, 15(15), 1710; https://doi.org/10.3390/agriculture15151710 (registering DOI) - 7 Aug 2025
Abstract
Planting patterns and irrigation amounts are key factors affecting maize yield. This study adopted a two-factor experimental design, with planting pattern as the main plot and irrigation amount as the subplot, to investigate the effects of irrigation levels under different planting patterns (including [...] Read more.
Planting patterns and irrigation amounts are key factors affecting maize yield. This study adopted a two-factor experimental design, with planting pattern as the main plot and irrigation amount as the subplot, to investigate the effects of irrigation levels under different planting patterns (including uniform row spacing and alternating wide-narrow row spacing) on spring maize yield and water use efficiency in Xinjiang. Through this approach, the study examined the mechanisms by which planting pattern and irrigation amount influence maize growth, yield formation, and water use efficiency. Experiments conducted at the Agricultural Science Research Institute of the Ninth Division of Xinjiang Production and Construction Corps demonstrated that alternating wide-narrow row spacing combined with moderate irrigation (5400 m3/hm2) significantly optimized maize root distribution, improved water use efficiency, and increased leaf area index and net photosynthetic rate, thereby promoting dry matter accumulation and yield enhancement. In contrast, uniform row spacing under high irrigation levels increased yield but resulted in lower water use efficiency. The study also found that alternating wide-narrow row spacing enhanced maize nutrient absorption from the soil, particularly phosphorus utilization efficiency, by improving canopy structure and root expansion. This pattern exhibited comprehensive advantages in resource utilization, providing a theoretical basis and technical pathway for achieving water-saving and high-yield maize production in arid regions, which holds significant importance for promoting sustainable agricultural development. Full article
(This article belongs to the Section Crop Production)
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14 pages, 871 KiB  
Article
Low-Cost Production of Brazilian Mahogany Clones Based on Indole-3-Butyric Acid Use, Clonal Mini-Hedge Nutrition and Vegetative Propagule Type
by Rafael Barbosa Diógenes Lienard, Annanda Souza de Campos, Lucas Graciolli Savian, Barbara Valentim de Oliveira, Felippe Coelho de Souza and Paulo André Trazzi
Forests 2025, 16(8), 1292; https://doi.org/10.3390/f16081292 (registering DOI) - 7 Aug 2025
Abstract
Swietenia macrophylla King, commonly known as Brazilian mahogany, is a high-value neotropical tree species currently threatened due to intensive logging in previous decades. Technologies aimed at clonal production are essential for this species’ conservation and sustainable use at times of climate change and [...] Read more.
Swietenia macrophylla King, commonly known as Brazilian mahogany, is a high-value neotropical tree species currently threatened due to intensive logging in previous decades. Technologies aimed at clonal production are essential for this species’ conservation and sustainable use at times of climate change and increasing demand for ecological restoration. The aim of the present study is to develop a low-cost protocol for mahogany clonal propagation through mini-cutting by assessing clonal mini-hedge nutrition, vegetative propagule type and indole-3-butyric acid (IBA) application effects on rooting and early clone growth. The experiment was conducted in nursery under controlled conditions based on using basal and apical mini-cuttings rooted in a low-cost mini-greenhouse subjected to three nutrient solution concentrations (50%, 100%, and 200%) and five IBA doses (0–8000 ppm). The mini-cutting technique proved efficient and led to over 90% survival after the hardening phase. The 200% nutrient solution concentration allowed balanced performance between cutting types and optimized clonal yield. IBA concentration at 4000 ppm accounted for higher root percentages at the bottom of the tube and the trend towards higher dry biomass production at 160 days. The results highlighted mini-cutting’s potential as a viable mahogany conservation and sustainable production technique. It also supported tropical forestry sector adaptation to challenges posed by climate change. Full article
16 pages, 755 KiB  
Article
Effects of Dietary Tannic Acid and Tea Polyphenol Supplementation on Rumen Fermentation, Methane Emissions, Milk Protein Synthesis and Microbiota in Cows
by Rong Zhao, Jiajin Sun, Yitong Lin, Haichao Yan, Shiyue Zhang, Wenjie Huo, Lei Chen, Qiang Liu, Cong Wang and Gang Guo
Microorganisms 2025, 13(8), 1848; https://doi.org/10.3390/microorganisms13081848 (registering DOI) - 7 Aug 2025
Abstract
To develop sustainable strategies for mitigating ruminal methanogenesis and improving nitrogen efficiency in dairy systems, this study investigated how low-dose tannic acid (T), tea polyphenols (TP), and their combination (T+TP; 50:50) modulate rumen microbiota and function. A sample of Holstein cows were given [...] Read more.
To develop sustainable strategies for mitigating ruminal methanogenesis and improving nitrogen efficiency in dairy systems, this study investigated how low-dose tannic acid (T), tea polyphenols (TP), and their combination (T+TP; 50:50) modulate rumen microbiota and function. A sample of Holstein cows were given four dietary treatments: (1) control (basal diet); (2) T (basal diet + 0.4% DM tannic acid); (3) TP (basal diet + 0.4% DM tea polyphenols); and (4) T+TP (basal diet + 0.2% DM tannic acid + 0.2% DM tea polyphenols). We comprehensively analyzed rumen fermentation, methane production, nutrient digestibility, milk parameters, and microbiota dynamics. Compared with the control group, all diets supplemented with additives significantly reduced enteric methane production (13.68% for T, 11.40% for TP, and 10.89% for T+TP) and significantly increased milk protein yield. The crude protein digestibility significantly increased in the T group versus control. The results did not impair rumen health or fiber digestion. Critically, microbiota analysis revealed treatment-specific modulation: the T group showed decreased Ruminococcus flavefaciens abundance, while all tannin treatments reduced abundances of Ruminococcus albus and total methanogens. These microbial shifts corresponded with functional outcomes—most notably, the T+TP synergy drove the largest reductions in rumen ammonia-N (34.5%) and milk urea nitrogen (21.1%). Supplementation at 0.4% DM, particularly the T+TP combination, effectively enhances nitrogen efficiency and milk protein synthesis while reducing methane emissions through targeted modulation of key rumen microbiota populations, suggesting potential sustainability benefits linked to altered rumen fermentation. Full article
(This article belongs to the Section Veterinary Microbiology)
43 pages, 15193 KiB  
Article
Bio-Mitigation of Sulfate Attack and Enhancement of Crack Self-Healing in Sustainable Concrete Using Bacillus megaterium and sphaericus Bacteria
by Ibrahim AbdElFattah, Seleem S. E. Ahmad, Ahmed A. Elakhras, Ahmed A. Elshami, Mohamed A. R. Elmahdy and Attitou Aboubakr
Infrastructures 2025, 10(8), 205; https://doi.org/10.3390/infrastructures10080205 (registering DOI) - 7 Aug 2025
Abstract
Concrete cracks and sulfate degradation severely compromise structural durability, highlighting the need for sustainable solutions to enhance longevity and minimize environmental impact. This study assesses the efficacy of bacterial self-healing technology utilizing Bacillus megaterium (BM) and Bacillus sphaericus (BS) in enhancing the resistance [...] Read more.
Concrete cracks and sulfate degradation severely compromise structural durability, highlighting the need for sustainable solutions to enhance longevity and minimize environmental impact. This study assesses the efficacy of bacterial self-healing technology utilizing Bacillus megaterium (BM) and Bacillus sphaericus (BS) in enhancing the resistance of concrete to sulfate attacks and improving its mechanical properties. Bacterial suspensions (1% and 2.5% of cement weight) were mixed with concrete containing silica fume or fly ash (10% of cement weight) and cured in freshwater or sulfate solutions (2%, 5%, and 10% concentrations). Specimens were tested for compressive strength, flexural strength, and microstructure using a Scanning Electron Microscope (SEM), Energy-Dispersive X-ray Spectroscopy (EDS), and X-ray diffraction (XRD) at various ages. The results indicate that a 2.5% bacterial content yielded the best performance, with BM surpassing BS, enhancing compressive strength by up to 41.3% and flexural strength by 52.3% in freshwater-cured samples. Although sulfate exposure initially improved early-age strength by 1.97% at 7 days, it led to an 8.5% loss at 120 days. Bacterial inclusion mitigated sulfate damage through microbially induced calcium carbonate precipitation (MICP), sealing cracks, and bolstering durability. Cracked specimens treated with BM recovered up to 93.1% of their original compressive strength, promoting sustainable, sulfate-resistant, self-healing concrete for more resilient infrastructure. Full article
(This article belongs to the Section Infrastructures Materials and Constructions)
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20 pages, 1149 KiB  
Article
Assessment of Biomethane Potential from Waste Activated Sludge in Swine Wastewater Treatment and Its Co-Digestion with Swine Slurry, Water Lily, and Lotus
by Sartika Indah Amalia Sudiarto, Hong Lim Choi, Anriansyah Renggaman and Arumuganainar Suresh
AgriEngineering 2025, 7(8), 254; https://doi.org/10.3390/agriengineering7080254 (registering DOI) - 7 Aug 2025
Abstract
Waste activated sludge (WAS), a byproduct of livestock wastewater treatment, poses significant disposal challenges due to its low biodegradability and potential environmental impact. Anaerobic digestion (AD) offers a sustainable approach for methane recovery and sludge stabilization. This study evaluates the biomethane potential (BMP) [...] Read more.
Waste activated sludge (WAS), a byproduct of livestock wastewater treatment, poses significant disposal challenges due to its low biodegradability and potential environmental impact. Anaerobic digestion (AD) offers a sustainable approach for methane recovery and sludge stabilization. This study evaluates the biomethane potential (BMP) of WAS and its co-digestion with swine slurry (SS), water lily (Nymphaea spp.), and lotus (Nelumbo nucifera) shoot biomass to enhance methane yield. Batch BMP assays were conducted at substrate-to-inoculum (S/I) ratios of 1.0 and 0.5, with methane production kinetics analyzed using the modified Gompertz model. Mono-digestion of WAS yielded 259.35–460.88 NmL CH4/g VSadded, while co-digestion with SS, water lily, and lotus increased yields by 14.89%, 10.97%, and 16.89%, respectively, surpassing 500 NmL CH4/g VSadded. All co-digestion combinations exhibited synergistic effects (α > 1), enhancing methane production beyond individual substrate contributions. Lower S/I ratios improved methane yields and biodegradability, highlighting the role of inoculum availability. Co-digestion reduced the lag phase limitations of WAS and plant biomass, improving process efficiency. These findings demonstrate that co-digesting WAS with nutrient-rich co-substrates optimizes biogas production, supporting sustainable sludge management and renewable energy recovery in livestock wastewater treatment systems. Full article
(This article belongs to the Section Sustainable Bioresource and Bioprocess Engineering)
22 pages, 4027 KiB  
Article
Parameter Sensitivity Analysis and Irrigation Regime Optimization for Jujube Trees in Arid Regions Using the WOFOST Model
by Shihao Sun, Yingjie Ma, Pengrui Ai, Ming Hong and Zhenghu Ma
Agriculture 2025, 15(15), 1705; https://doi.org/10.3390/agriculture15151705 (registering DOI) - 7 Aug 2025
Abstract
In arid regions, water scarcity and soil potassium destruction are major constraints on the sustainable development of the jujube industry. In this regard, the use of crop models can compensate for time-consuming and costly field trials to screen for better irrigation regimes, but [...] Read more.
In arid regions, water scarcity and soil potassium destruction are major constraints on the sustainable development of the jujube industry. In this regard, the use of crop models can compensate for time-consuming and costly field trials to screen for better irrigation regimes, but their predictive accuracy is often compromised by parameter uncertainty. To address this issue, we utilized data from a three-year (2022–2024) field trial (with irrigation at 50%, 75%, and 100% of evapotranspiration and potassium applications of 120, 180, and 240 kg/ha) to simulate the growth process of jujube trees in arid regions using the WOFOST model. In this study, parameter sensitivity analyses were conducted to determine that photosynthetic capacity maximization (Amax), the potassium nutrition index (Kstatus), the water stress factor (SWF), the water–potassium photosynthetic coefficient of synergy (α), and potassium partitioning weight coefficients (βi) were the important parameters affecting the simulated growth process of the crop. Path analysis using segmented structural equations also showed that water stress factor (SWF) and potassium nutrition index (Kstatus) indirectly controlled yield by significantly affecting photosynthesis (path coefficients: 0.72 and 0.75, respectively). The ability of the crop model to simulate the growth process and yield of jujube trees was improved by the introduction of water and potassium parameters (R2 = 0.94–0.96, NRMSE = 4.1–12.2%). The subsequent multi-objective optimization of yield and crop water productivity of dates under different combinations of water and potassium treatments under a bi-objective optimization model based on the NSGA-II algorithm showed that the optimal strategy was irrigation at 80% ETc combined with 300 kg/ha of potassium application. This management model ensures yield and maximizes crop water use efficiency (CWP), thus providing a scientific and efficient irrigation and fertilization regime for jujube trees in arid zones. Full article
(This article belongs to the Section Crop Production)
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34 pages, 3764 KiB  
Review
Research Progress and Applications of Artificial Intelligence in Agricultural Equipment
by Yong Zhu, Shida Zhang, Shengnan Tang and Qiang Gao
Agriculture 2025, 15(15), 1703; https://doi.org/10.3390/agriculture15151703 - 7 Aug 2025
Abstract
With the growth of the global population and the increasing scarcity of arable land, traditional agricultural production is confronted with multiple challenges, such as efficiency improvement, precision operation, and sustainable development. The progressive advancement of artificial intelligence (AI) technology has created a transformative [...] Read more.
With the growth of the global population and the increasing scarcity of arable land, traditional agricultural production is confronted with multiple challenges, such as efficiency improvement, precision operation, and sustainable development. The progressive advancement of artificial intelligence (AI) technology has created a transformative opportunity for the intelligent upgrade of agricultural equipment. This article systematically presents recent progress in computer vision, machine learning (ML), and intelligent sensing. The key innovations are highlighted in areas such as object detection and recognition (e.g., a K-nearest neighbor (KNN) achieved 98% accuracy in distinguishing vibration signals across operation stages); autonomous navigation and path planning (e.g., a deep reinforcement learning (DRL)-optimized task planner for multi-arm harvesting robots reduced execution time by 10.7%); state perception (e.g., a multilayer perceptron (MLP) yielded 96.9% accuracy in plug seedling health classification); and precision control (e.g., an intelligent multi-module coordinated control system achieved a transplanting efficiency of 5000 plants/h). The findings reveal a deep integration of AI models with multimodal perception technologies, significantly improving the operational efficiency, resource utilization, and environmental adaptability of agricultural equipment. This integration is catalyzing the transition toward intelligent, automated, and sustainable agricultural systems. Nevertheless, intelligent agricultural equipment still faces technical challenges regarding data sample acquisition, adaptation to complex field environments, and the coordination between algorithms and hardware. Looking ahead, the convergence of digital twin (DT) technology, edge computing, and big data-driven collaborative optimization is expected to become the core of next-generation intelligent agricultural systems. These technologies have the potential to overcome current limitations in perception and decision-making, ultimately enabling intelligent management and autonomous decision-making across the entire agricultural production chain. This article aims to provide a comprehensive foundation for advancing agricultural modernization and supporting green, sustainable development. Full article
(This article belongs to the Section Agricultural Technology)
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27 pages, 1523 KiB  
Article
Reinforcement Learning-Based Agricultural Fertilization and Irrigation Considering N2O Emissions and Uncertain Climate Variability
by Zhaoan Wang, Shaoping Xiao, Jun Wang, Ashwin Parab and Shivam Patel
AgriEngineering 2025, 7(8), 252; https://doi.org/10.3390/agriengineering7080252 - 7 Aug 2025
Abstract
Nitrous oxide (N2O) emissions from agriculture are rising due to increased fertilizer use and intensive farming, posing a major challenge for climate mitigation. This study introduces a novel reinforcement learning (RL) framework to optimize farm management strategies that balance [...] Read more.
Nitrous oxide (N2O) emissions from agriculture are rising due to increased fertilizer use and intensive farming, posing a major challenge for climate mitigation. This study introduces a novel reinforcement learning (RL) framework to optimize farm management strategies that balance crop productivity with environmental impact, particularly N2O emissions. By modeling agricultural decision-making as a partially observable Markov decision process (POMDP), the framework accounts for uncertainties in environmental conditions and observational data. The approach integrates deep Q-learning with recurrent neural networks (RNNs) to train adaptive agents within a simulated farming environment. A Probabilistic Deep Learning (PDL) model was developed to estimate N2O emissions, achieving a high Prediction Interval Coverage Probability (PICP) of 0.937 within a 95% confidence interval on the available dataset. While the PDL model’s generalizability is currently constrained by the limited observational data, the RL framework itself is designed for broad applicability, capable of extending to diverse agricultural practices and environmental conditions. Results demonstrate that RL agents reduce N2O emissions without compromising yields, even under climatic variability. The framework’s flexibility allows for future integration of expanded datasets or alternative emission models, ensuring scalability as more field data becomes available. This work highlights the potential of artificial intelligence to advance climate-smart agriculture by simultaneously addressing productivity and sustainability goals in dynamic real-world settings. Full article
(This article belongs to the Special Issue Implementation of Artificial Intelligence in Agriculture)
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21 pages, 3488 KiB  
Article
Effects of Continuous Saline Water Irrigation on Soil Salinization Characteristics and Dryland Jujube Tree
by Qiao Zhao, Mingliang Xin, Pengrui Ai and Yingjie Ma
Agronomy 2025, 15(8), 1898; https://doi.org/10.3390/agronomy15081898 - 7 Aug 2025
Abstract
The sustainable utilization of saline water resources represents an effective strategy for alleviating water scarcity in arid regions. However, the mechanisms by which prolonged saline water irrigation influences soil salinization and dryland crop growth are not yet fully understood. This study examined the [...] Read more.
The sustainable utilization of saline water resources represents an effective strategy for alleviating water scarcity in arid regions. However, the mechanisms by which prolonged saline water irrigation influences soil salinization and dryland crop growth are not yet fully understood. This study examined the effects of six irrigation water salinity levels (CK: 0.87 g·L−1, S1: 2 g·L−1, S2: 4 g·L−1, S3: 6 g·L−1, S4: 8 g·L−1, S5: 10 g·L−1) on soil salinization dynamics and jujube growth during a three-year field experiment (2020–2022). The results showed that soil salinity within the 0–1 m profile significantly increased with rising irrigation water salinity and prolonged irrigation duration, with the 0–0.4 m layer accounting for 50.27–74.95% of the total salt accumulation. A distinct unimodal salt distribution was observed in the 0.3–0.6 m soil zone, with the salinity peak shifting downward from 0.4 to 0.5 m over time. Meanwhile, soil pH and sodium adsorption ratio (SAR) increased steadily over the study period. The dominant hydrochemical type shifted from SO42−-Ca2+·Mg2+ to Cl-Na+·Mg2+. Crop performance exhibited a nonlinear response to irrigation salinity levels. Low salinity (2 g·L−1) significantly enhanced plant height, stem diameter, leaf area index (LAI), vitamin C content, and yield, with improvements of up to 12.11%, 3.96%, 16.67%, 16.24%, and 16.52% in the early years. However, prolonged exposure to saline irrigation led to significant declines in both plant growth and water productivity (WP) by 2022. Under high-salinity conditions (S5), yield decreased by 16.75%, while WP declined by more than 30%. To comprehensively evaluate the trade-off between economic effects and soil environment, the entropy weight TOPSIS method was employed to identify S1 as the optimal irrigation treatment for the 2020–2021 period and control (CK) as the optimal treatment for 2022. Through fitting analysis, the optimal irrigation water salinity levels over 3 years were determined to be 2.75 g·L−1, 2.49 g·L−1, and 0.87 g·L−1, respectively. These findings suggest that short-term irrigation of jujube trees with saline water at concentrations ≤ 3 g·L−1 is agronomically feasible. Full article
(This article belongs to the Section Water Use and Irrigation)
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35 pages, 1831 KiB  
Review
Pesticide Degradation: Impacts on Soil Fertility and Nutrient Cycling
by Muhammad Yasir, Abul Hossain and Anubhav Pratap-Singh
Environments 2025, 12(8), 272; https://doi.org/10.3390/environments12080272 - 7 Aug 2025
Abstract
The widespread use of pesticides in modern agriculture has significantly enhanced food production by managing pests and diseases; however, their degradation in soil can lead to unintended consequences for soil fertility and nutrient cycling. This review explores the mechanisms of pesticide degradation, both [...] Read more.
The widespread use of pesticides in modern agriculture has significantly enhanced food production by managing pests and diseases; however, their degradation in soil can lead to unintended consequences for soil fertility and nutrient cycling. This review explores the mechanisms of pesticide degradation, both abiotic and biotic, and the soil factors influencing these processes. It critically examines how degradation products impact soil microbial communities, organic matter decomposition, and key nutrient cycles, including nitrogen, phosphorus, potassium, and micronutrients. This review highlights emerging evidence linking pesticide residues with altered enzymatic activity, disrupted microbial populations, and reduced nutrient bioavailability, potentially compromising soil structure, water retention, and long-term productivity. Additionally, it discusses the broader environmental and agricultural implications, including decreased crop yields, biodiversity loss, and groundwater contamination. Sustainable management strategies such as bioremediation, the use of biochar, eco-friendly pesticides, and integrated pest management (IPM) are evaluated for mitigating these adverse effects. Finally, this review outlines future research directions emphasizing long-term studies, biotechnology innovations, and predictive modeling to support resilient agroecosystems. Understanding the intricate relationship between pesticide degradation and soil health is crucial to ensuring sustainable agriculture and food security. Full article
(This article belongs to the Special Issue Coping with Climate Change: Fate of Nutrients and Pollutants in Soil)
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19 pages, 1717 KiB  
Article
A Multifaceted Approach to Optimizing Processed Tomato Production: Investigating the Combined Effects of Biostimulants and Reduced Nitrogen Fertilization
by Michela Farneselli, Lara Reale, Beatrice Falcinelli, Muhammad Zubair Akram, Stefano Cimarelli, Eleonore Cinti, Michela Paglialunga, Flavia Carbone, Euro Pannacci and Francesco Tei
Horticulturae 2025, 11(8), 931; https://doi.org/10.3390/horticulturae11080931 - 7 Aug 2025
Abstract
Excessive nitrogen (N) fertilizer usage in agriculture has prompted the exploration of sustainable strategies to enhance nitrogen use efficiency (NUE) while maintaining crop yield and quality. Processed tomatoes (Solanum lycopersicum L.) were grown for two years (2023 and 2024) following a two-way [...] Read more.
Excessive nitrogen (N) fertilizer usage in agriculture has prompted the exploration of sustainable strategies to enhance nitrogen use efficiency (NUE) while maintaining crop yield and quality. Processed tomatoes (Solanum lycopersicum L.) were grown for two years (2023 and 2024) following a two-way factorial randomized complete block (RCBD) design, considering three biostimulants and three N regimes as two factors, to assess their morphophysiological, biochemical, anatomical and yield performances. Nitrogen application significantly influenced biomass accumulation, the leaf area index (LAI), nitrogen uptake and yield with notable comparable values between reduced and optimal nitrogen dose, indicating improved nitrogen use efficiency. Biostimulants showed limited effects alone but enhanced plant performance under reduced nitrogen conditions, particularly improving chlorophyll content, crop growth, N uptake, yield and anatomical adaptations. Moreover, compared to 2024, biostimulant application enhanced tomato growth more evidently in 2023 due to environmental variations, likely due to the occurrence of stress conditions. Importantly, biostimulants, together with N regimes, i.e., optimal and reduced doses, showed improved anatomical traits, especially regarding leaf thickness and thickness between the two epidermises, indicating adaptive responses that may support sustained productivity under N-limited conditions. Among the biostimulants used, the processed tomatoes responded better to protein hydrolysate and endophytic N-fixing bacteria than to seaweed extract. These findings suggest that although biostimulants alone were not affected, integrating them with reduced N fertilization provides a viable strategy for optimizing tomato production, conserving resources and minimizing the environmental impact without compromising yield or quality. Full article
(This article belongs to the Special Issue Effects of Biostimulants on Horticultural Crop Production)
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25 pages, 1677 KiB  
Review
Sustainable, Targeted, and Cost-Effective Laccase-Based Bioremediation Technologies for Antibiotic Residues in the Ecosystem: A Comprehensive Review
by Rinat Ezra, Gulamnabi Vanti and Segula Masaphy
Biomolecules 2025, 15(8), 1138; https://doi.org/10.3390/biom15081138 - 7 Aug 2025
Abstract
Widespread antibiotic residues are accumulating in the environment, potentially causing adverse effects for humans, animals, and the ecosystem, including an increase in antibiotic-resistant bacteria, resulting in worldwide concern. There are various commonly used physical, chemical, and biological treatments for the degradation of antibiotics. [...] Read more.
Widespread antibiotic residues are accumulating in the environment, potentially causing adverse effects for humans, animals, and the ecosystem, including an increase in antibiotic-resistant bacteria, resulting in worldwide concern. There are various commonly used physical, chemical, and biological treatments for the degradation of antibiotics. However, the elimination of toxic end products generated by physicochemical methods and the need for industrial applications pose significant challenges. Hence, environmentally sustainable, green, and readily available approaches for the transformation and degradation of these antibiotic compounds are being sought. Herein, we review the impact of sustainable fungal laccase-based bioremediation strategies. Fungal laccase enzyme is considered one of the most active enzymes for biotransformation and biodegradation of antibiotic residue in vitro. For industrial applications, the low laccase yields in natural and genetically modified hosts may constitute a bottleneck. Methods to screen for high-laccase-producing sources, optimizing cultivation conditions, and identifying key genes and metabolites involved in extracellular laccase activity are reviewed. These include advanced transcriptomics, proteomics, and metagenomics technologies, as well as diverse laccase-immobilization technologies with different inert carrier/support materials improving enzyme performance whilst shifting from experimental assays to in situ monitoring of residual toxicity. Still, more basic and applied research on laccase-mediated bioremediation of pharmaceuticals, especially antibiotics that are recalcitrant and prevalent, is needed. Full article
(This article belongs to the Special Issue Recent Advances in Laccases and Laccase-Based Bioproducts)
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17 pages, 7385 KiB  
Article
Microbial Alliance of Paenibacillus sp. SPR11 and Bradyrhizobium yuanmingense PR3 Enhances Nitrogen Fixation, Yield, and Salinity Tolerance in Black Gram Under Saline, Nutrient-Depleted Soils
by Praveen Kumar Tiwari, Anchal Kumar Srivastava, Rachana Singh and Alok Kumar Srivastava
Nitrogen 2025, 6(3), 66; https://doi.org/10.3390/nitrogen6030066 - 7 Aug 2025
Abstract
Salinity is a major abiotic stress limiting black gram (Vigna mungo) productivity, particularly in arid and semi-arid regions. Saline soils negatively impact plant growth, nodulation, nitrogen fixation, and yield. This study evaluated the efficacy of co-inoculating salt-tolerant plant growth-promoting bacteria Paenibacillus [...] Read more.
Salinity is a major abiotic stress limiting black gram (Vigna mungo) productivity, particularly in arid and semi-arid regions. Saline soils negatively impact plant growth, nodulation, nitrogen fixation, and yield. This study evaluated the efficacy of co-inoculating salt-tolerant plant growth-promoting bacteria Paenibacillus sp. SPR11 and Bradyrhizobium yuanmingense PR3 on black gram performance under saline field conditions (EC: 8.87 dS m−1; pH: 8.37) with low organic carbon (0.6%) and nutrient deficiencies. In vitro assays demonstrated the biocontrol potential of SPR11, inhibiting Fusarium oxysporum and Macrophomina phaseolina by 76% and 62%, respectively. Germination assays and net house experiments under 300 mM NaCl stress showed that co-inoculation significantly improved physiological traits, including germination rate, root length (61.39%), shoot biomass (59.95%), and nitrogen fixation (52.4%) in nitrogen-free media. Field trials further revealed enhanced stress tolerance markers: chlorophyll content increased by 54.74%, proline by 50.89%, and antioxidant enzyme activities (SOD, CAT, PAL) were significantly upregulated. Electrolyte leakage was reduced by 55.77%, indicating improved membrane stability. Agronomic performance also improved, with co-inoculated plants showing increased root length (7.19%), grain yield (15.55 q ha−1; 77.04% over control), total biomass (26.73 q ha−1; 57.06%), and straw yield (8.18 q ha−1). Pod number, seed count, and seed weight were also enhanced. Nutrient analysis showed elevated uptake of nitrogen, phosphorus, potassium, and key micronutrients (Zn, Fe) in both grain and straw. To the best of our knowledge, this is the very first field-based report demonstrating the synergistic benefits of co-inoculating Paenibacillus sp. SPR11 and Bradyrhizobium yuanmingense PR3 in black gram under saline, nutrient-poor conditions without external nitrogen inputs. The results highlight a sustainable strategy to enhance legume productivity and resilience in salt-affected soils. Full article
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17 pages, 7119 KiB  
Article
Rapid-Optimized Process Parameters of 1080 Carbon Steel Additively Manufactured via Laser Powder Bed Fusion on High-Throughput Mechanical Property Testing
by Jianyu Feng, Meiling Jiang, Guoliang Huang, Xudong Wu and Ke Huang
Materials 2025, 18(15), 3705; https://doi.org/10.3390/ma18153705 - 6 Aug 2025
Abstract
To ensure the sustainability of alloy-based strategies, both compositional design and processing routes must be simplified. Metal additive manufacturing (AM), with its exceptionally rapid, non-equilibrium solidification, offers a unique platform to produce tailored microstructures in simple alloys that deliver superior mechanical properties. In [...] Read more.
To ensure the sustainability of alloy-based strategies, both compositional design and processing routes must be simplified. Metal additive manufacturing (AM), with its exceptionally rapid, non-equilibrium solidification, offers a unique platform to produce tailored microstructures in simple alloys that deliver superior mechanical properties. In this study, we employ laser powder bed fusion (LPBF) to fabricate 1080 plain carbon steel, a binary alloy comprising only iron and carbon. Deviating from conventional process optimization focusing primarily on density, we optimize LPBF parameters for mechanical performance. We systematically varied key parameters (laser power and scan speed) to produce batches of tensile specimens, which were then evaluated on a high-throughput mechanical testing platform (HTP). Using response surface methodology (RSM), we developed predictive models correlating these parameters with yield strength (YS) and elongation. The RSM models identified optimal and suboptimal parameter sets. Specimens printed under the predicted optimal conditions achieved YS of 1543.5 MPa and elongation of 7.58%, closely matching RSM predictions (1595.3 MPa and 8.32%) with deviations of −3.25% and −8.89% for YS and elongation, respectively, thus validating model accuracy. Comprehensive microstructural characterization, including metallographic analysis and fracture surface examination, revealed the microstructural origins of performance differences and the underlying strengthening mechanisms. This methodology enables rapid evaluation and optimization of LPBF parameters for 1080 carbon steel and can be generalized as an efficient framework for robust LPBF process development. Full article
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