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17 pages, 1323 KiB  
Article
The Effect of Nitrogen Fertilizer Placement and Timing on Winter Wheat Grain Yield and Protein Concentration
by Brent Ballagh, Anna Ballagh, Jacob Bushong and Daryl Brian Arnall
Agronomy 2025, 15(8), 1890; https://doi.org/10.3390/agronomy15081890 - 5 Aug 2025
Abstract
Nitrogen (N) fertilizer management in winter wheat production faces challenges from volatilization losses and sub-optimal application strategies. This is particularly problematic in the Southern Great Plains, where environmental conditions during top-dressing periods favor N losses. This study evaluated the effects of a fertilizer [...] Read more.
Nitrogen (N) fertilizer management in winter wheat production faces challenges from volatilization losses and sub-optimal application strategies. This is particularly problematic in the Southern Great Plains, where environmental conditions during top-dressing periods favor N losses. This study evaluated the effects of a fertilizer placement method, enhanced-efficiency fertilizers, and application timing on grain yield and protein concentration (GPC) across six site-years in Oklahoma (2016–2018). Treatments included broadcast applications of untreated urea and SuperU® (urease/nitrification inhibitor-treated urea). These were compared with subsurface placement using single-disc and double-disc drilling systems, applied at 67 kg N ha−1 during January, February, or March. Subsurface placement increased the grain yield by 324–391 kg ha−1 compared to broadcast applications at sites with favorable soil conditions. However, responses varied significantly across environments. Enhanced-efficiency fertilizers showed limited advantages over untreated urea. Benefits were most pronounced during February applications under conditions favoring volatilization losses. Application timing effects were more consistent for GPC than for the yield. Later applications (February–March) increased GPC by 0.8–1.2% compared to January applications. Treatment efficacy was strongly influenced by soil pH, equipment performance, and post-application environmental conditions. This indicates that N management benefits are highly site-specific. These findings demonstrate that subsurface placement can improve nitrogen use efficiency (NUE) under appropriate conditions. However, success depends on matching application strategies to local soil and environmental factors rather than adopting universal recommendations. Full article
(This article belongs to the Special Issue Fertility Management for Higher Crop Productivity)
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21 pages, 3832 KiB  
Article
Effects of Water Use Efficiency Combined with Advancements in Nitrogen and Soil Water Management for Sustainable Agriculture in the Loess Plateau, China
by Hafeez Noor, Fida Noor, Zhiqiang Gao, Majed Alotaibi and Mahmoud F. Seleiman
Water 2025, 17(15), 2329; https://doi.org/10.3390/w17152329 - 5 Aug 2025
Abstract
In China’s Loess Plateau, sustainable agricultural end products are affected by an insufficiency of water resources. Rising crop water use efficiency (WUE) through field management pattern improvement is a crucial plan of action to address this issue. However, there is no agreement among [...] Read more.
In China’s Loess Plateau, sustainable agricultural end products are affected by an insufficiency of water resources. Rising crop water use efficiency (WUE) through field management pattern improvement is a crucial plan of action to address this issue. However, there is no agreement among researchers on the most appropriate field management practices regarding WUE, which requires further integrated quantitative analysis. We conducted a meta-analysis by quantifying the effect of agricultural practices surrounding nitrogen (N) fertilizer management. The two experimental cultivars were Yunhan–20410 and Yunhan–618. The subplots included nitrogen 0 kg·ha−1 (N0), 90 kg·ha−1 (N90), 180 kg·ha−1 (N180), 210 kg·ha−1 (N210), and 240 kg·ha−1 (N240). Our results show that higher N rates (up to N210) enhanced water consumption during the node-flowering and flowering-maturity time periods. YH–618 showed higher water use during the sowing–greening and node-flowering periods but decreased use during the greening-node and flowering-maturity periods compared to YH–20410. The N210 treatment under YH–618 maximized water use efficiency (WUE). Increased N rates (N180–N210) decreased covering temperatures (Tmax, Tmin, Taver) during flowering, increasing the level of grain filling. Spike numbers rose with N application, with an off-peak at N210 for strong-gluten wheat. The 1000-grain weight was at first enhanced but decreased at the far end of N180–N210. YH–618 with N210 achieved a harvest index (HI) similar to that of YH–20410 with N180, while excessive N (N240) or water reduced the HI. Dry matter accumulation increased up to N210, resulting in earlier stabilization. Soil water consumption from wintering to jointing was strongly correlated with pre-flowering dry matter biological process and yield, while jointing–flowering water use was linked to post-flowering dry matter and spike numbers. Post-flowering dry matter accumulation was critical for yield, whereas spike numbers positively impacted yield but negatively affected 1000-grain weight. In conclusion, our results provide evidence for determining suitable integrated agricultural establishment strategies to ensure efficient water use and sustainable production in the Loess Plateau region. Full article
(This article belongs to the Special Issue Soil–Water Interaction and Management)
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13 pages, 1780 KiB  
Article
The Use of Sound Recorders to Remotely Measure Grass Intake Behaviour in Horses
by Daisy E. F. Taylor, Bryony E. Lancaster and Andrea D. Ellis
Animals 2025, 15(15), 2273; https://doi.org/10.3390/ani15152273 - 4 Aug 2025
Viewed by 77
Abstract
Visual observation to record grass intake is time-consuming and labour-intensive. Technological methods, such as activity monitors, have been used but only record head position. This study aimed to evaluate sound recorders attached to headcollars to acoustically measure grass intake behaviour in horses as [...] Read more.
Visual observation to record grass intake is time-consuming and labour-intensive. Technological methods, such as activity monitors, have been used but only record head position. This study aimed to evaluate sound recorders attached to headcollars to acoustically measure grass intake behaviour in horses as a low-cost alternative method. Pilot Study 1 assessed 6 × 11 min periods comparing bites/min and chews/min between video footage (VD) and sound recorders (SR). Grazing was identified audibly (SRear) and visually through soundwave pattern software (SRwav). Chew rates (SRear: 47 ± 5 chews/min, VD: 43 ± 4 chews/min) were similar between methods. Pilot Study 2 compared hourly grass intake times between SRwav and visual observation (VO) for two horses during a 3 h period. Results showed significant correlation between methods (rho = 0.99, p < 0.01, Spearman). The main study measured intake behaviour using SRwav and VO methods for three free-ranging horses during 3 h observation periods over multiple days, adding up to 3 × 24 h in winter and in spring (n = 48). Mean differences per period between SRwav and VO were 1.8% ± 3 s.d. Foraging duration per period measured with SRwav closely matched VO (r2 = 0.99, p < 0.001). Sound recorders accurately recorded grass intake time and chews in grazing horses during moderate weather conditions. Full article
(This article belongs to the Section Equids)
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21 pages, 7111 KiB  
Article
Seasonal Variation in Energy Balance, Evapotranspiration and Net Ecosystem Production in a Desert Ecosystem of Dengkou, Inner Mongolia, China
by Muhammad Zain Ul Abidin, Huijie Xiao, Sanaullah Magsi, Fang Hongxin, Komal Muskan, Phuocthoi Hoang and Muhammad Azher Hassan
Water 2025, 17(15), 2307; https://doi.org/10.3390/w17152307 - 3 Aug 2025
Viewed by 209
Abstract
This study investigates the seasonal dynamics of energy balance, evapotranspiration (ET), and Net Ecosystem Production (NEP) in the Dengkou desert ecosystem of Inner Mongolia, China. Using eddy covariance and meteorological data from 2019 to 2022, the research focuses on understanding how these processes [...] Read more.
This study investigates the seasonal dynamics of energy balance, evapotranspiration (ET), and Net Ecosystem Production (NEP) in the Dengkou desert ecosystem of Inner Mongolia, China. Using eddy covariance and meteorological data from 2019 to 2022, the research focuses on understanding how these processes interact in one of the world’s most water-limited environments. This arid research area received an average of 109.35 mm per annum precipitation over the studied period, classifying the region as a typical arid ecosystem. Seasonal patterns were observed in daily air temperature, with extremes ranging from −20.6 °C to 29.6 °C. Temporal variations in sensible heat flux (H), latent heat flux (LE), and net radiation (Rn) peaked during summer season. The average ground heat flux (G) was mostly positive throughout the observation period, indicating heat transmission from atmosphere to soil, but showed negative values during the winter season. The energy balance ratio for the studied period was in the range of 0.61 to 0.80, indicating challenges in achieving energy closure and ecological shifts. ET exhibited two annual peaks influenced by vegetation growth and climate change, with annual ET exceeding annual precipitation, except in 2021. Net ecosystem production (NEP) from 2019 to 2020 revealed that the Dengkou desert were a net source of carbon, indicating the carbon loss from the ecosystem. In 2021, the Dengkou ecosystem shifted to become a net carbon sink, effectively sequestrating carbon. However, this was sharply reversed in 2022, resulting in a significant net release of carbon. The study findings highlight the complex interactions between energy balance components, ET, and NEP in desert ecosystems, providing insights into sustainable water management and carbon neutrality strategies in arid regions under climate change effect. Full article
(This article belongs to the Special Issue The Observation and Modeling of Surface Air Hydrological Factors)
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19 pages, 18533 KiB  
Article
Modeling of Marine Assembly Logistics for an Offshore Floating Photovoltaic Plant Subject to Weather Dependencies
by Lu-Jan Huang, Simone Mancini and Minne de Jong
J. Mar. Sci. Eng. 2025, 13(8), 1493; https://doi.org/10.3390/jmse13081493 - 2 Aug 2025
Viewed by 111
Abstract
Floating solar technology has gained significant attention as part of the global expansion of renewable energy due to its potential for installation in underutilized water bodies. Several countries, including the Netherlands, have initiated efforts to extend this technology from inland freshwater applications to [...] Read more.
Floating solar technology has gained significant attention as part of the global expansion of renewable energy due to its potential for installation in underutilized water bodies. Several countries, including the Netherlands, have initiated efforts to extend this technology from inland freshwater applications to open offshore environments, particularly within offshore wind farm areas. This development is motivated by the synergistic benefits of increasing site energy density and leveraging the existing offshore grid infrastructure. The deployment of offshore floating photovoltaic (OFPV) systems involves assembling multiple modular units in a marine environment, introducing operational risks that may give rise to safety concerns. To mitigate these risks, weather windows must be considered prior to the task execution to ensure continuity between weather-sensitive activities, which can also lead to additional time delays and increased costs. Consequently, optimizing marine logistics becomes crucial to achieving the cost reductions necessary for making OFPV technology economically viable. This study employs a simulation-based approach to estimate the installation duration of a 5 MWp OFPV plant at a Dutch offshore wind farm site, started in different months and under three distinct risk management scenarios. Based on 20 years of hindcast wave data, the results reveal the impacts of campaign start months and risk management policies on installation duration. Across all the scenarios, the installation duration during the autumn and winter period is 160% longer than the one in the spring and summer period. The average installation durations, based on results from 12 campaign start months, are 70, 80, and 130 days for the three risk management policies analyzed. The result variation highlights the additional time required to mitigate operational risks arising from potential discontinuity between highly interdependent tasks (e.g., offshore platform assembly and mooring). Additionally, it is found that the weather-induced delays are mainly associated with the campaigns of pre-laying anchors and platform and mooring line installation compared with the other campaigns. In conclusion, this study presents a logistics modeling methodology for OFPV systems, demonstrated through a representative case study based on a state-of-the-art truss-type design. The primary contribution lies in providing a framework to quantify the performance of OFPV installation strategies at an early design stage. The findings of this case study further highlight that marine installation logistics are highly sensitive to local marine conditions and the chosen installation strategy, and should be integrated early in the OFPV design process to help reduce the levelized cost of electricity. Full article
(This article belongs to the Special Issue Design, Modeling, and Development of Marine Renewable Energy Devices)
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21 pages, 6618 KiB  
Article
Comparison of Deep Learning Models for LAI Simulation and Interpretable Hydrothermal Coupling in the Loess Plateau
by Junpo Yu, Yajun Si, Wen Zhao, Zeyu Zhou, Jiming Jin, Wenjun Yan, Xiangyu Shao, Zhixiang Xu and Junwei Gan
Plants 2025, 14(15), 2391; https://doi.org/10.3390/plants14152391 - 2 Aug 2025
Viewed by 202
Abstract
As the world’s largest loess deposit region, the Loess Plateau’s vegetation dynamics are crucial for its regional water–heat balance and ecosystem functioning. Leaf Area Index (LAI) serves as a key indicator bridging canopy architecture and plant physiological activities. Existing studies have made significant [...] Read more.
As the world’s largest loess deposit region, the Loess Plateau’s vegetation dynamics are crucial for its regional water–heat balance and ecosystem functioning. Leaf Area Index (LAI) serves as a key indicator bridging canopy architecture and plant physiological activities. Existing studies have made significant advancements in simulating LAI, yet accurate LAI simulation remains challenging. To address this challenge and gain deeper insights into the environmental controls of LAI, this study aims to accurately simulate LAI in the Loess Plateau using deep learning models and to elucidate the spatiotemporal influence of soil moisture and temperature on LAI dynamics. For this purpose, we used three deep learning models, namely Artificial Neural Network (ANN), Long Short-Term Memory (LSTM), and Interpretable Multivariable (IMV)-LSTM, to simulate LAI in the Loess Plateau, only using soil moisture and temperature as inputs. Results indicated that our approach outperformed traditional models and effectively captured LAI variations across different vegetation types. The attention analysis revealed that soil moisture mainly influenced LAI in the arid northwest and temperature was the predominant effect in the humid southeast. Seasonally, soil moisture was crucial in spring and summer, notably in grasslands and croplands, whereas temperature dominated in autumn and winter. Notably, forests had the longest temperature-sensitive periods. As LAI increased, soil moisture became more influential, and at peak LAI, both factors exerted varying controls on different vegetation types. These findings demonstrated the strength of deep learning for simulating vegetation–climate interactions and provided insights into hydrothermal regulation mechanisms in semiarid regions. Full article
(This article belongs to the Section Plant Modeling)
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27 pages, 1948 KiB  
Article
Real-World Performance and Economic Evaluation of a Residential PV Battery Energy Storage System Under Variable Tariffs: A Polish Case Study
by Wojciech Goryl
Energies 2025, 18(15), 4090; https://doi.org/10.3390/en18154090 - 1 Aug 2025
Viewed by 295
Abstract
This paper presents an annual, real-world evaluation of the performance and economics of a residential photovoltaic (PV) system coupled with a battery energy storage system (BESS) in southern Poland. The system, monitored with 5 min resolution, operated under time-of-use (TOU) electricity tariffs. Seasonal [...] Read more.
This paper presents an annual, real-world evaluation of the performance and economics of a residential photovoltaic (PV) system coupled with a battery energy storage system (BESS) in southern Poland. The system, monitored with 5 min resolution, operated under time-of-use (TOU) electricity tariffs. Seasonal variation was significant; self-sufficiency exceeded 90% in summer, while winter conditions increased grid dependency. The hybrid system reduced electricity costs by over EUR 1400 annually, with battery operation optimized for high-tariff periods. Comparative analysis of three configurations—grid-only, PV-only, and PV + BESS—demonstrated the economic advantage of the integrated solution, with the shortest payback period (9.0 years) achieved with financial support. However, grid voltage instability during high PV production led to inverter shutdowns, highlighting limitations in the infrastructure. This study emphasizes the importance of tariff strategies, environmental conditions, and voltage control when designing residential PV-BESS systems. Full article
(This article belongs to the Special Issue Design, Analysis and Operation of Renewable Energy Systems)
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23 pages, 4456 KiB  
Article
Assessing Climate Change Impacts on Groundwater Recharge and Storage Using MODFLOW in the Akhangaran River Alluvial Aquifer, Eastern Uzbekistan
by Azam Kadirkhodjaev, Dmitriy Andreev, Botir Akramov, Botirjon Abdullaev, Zilola Abdujalilova, Zulkhumar Umarova, Dilfuza Nazipova, Izzatullo Ruzimov, Shakhriyor Toshev, Erkin Anorboev, Nodirjon Rakhimov, Farrukh Mamirov, Inessa Gracheva and Samrit Luoma
Water 2025, 17(15), 2291; https://doi.org/10.3390/w17152291 - 1 Aug 2025
Viewed by 432
Abstract
A shallow quaternary sedimentary aquifer within the river alluvial deposits of eastern Uzbekistan is increasingly vulnerable to the impacts of climate change and anthropogenic activities. Despite its essential role in supplying water for domestic, agricultural, and industrial purposes, the aquifer system remains poorly [...] Read more.
A shallow quaternary sedimentary aquifer within the river alluvial deposits of eastern Uzbekistan is increasingly vulnerable to the impacts of climate change and anthropogenic activities. Despite its essential role in supplying water for domestic, agricultural, and industrial purposes, the aquifer system remains poorly understood. This study employed a three-dimensional MODFLOW-based groundwater flow model to assess climate change impacts on water budget components under the SSP5-8.5 scenario for 2020–2099. Model calibration yielded RMSE values between 0.25 and 0.51 m, indicating satisfactory performance. Simulations revealed that lateral inflows from upstream and side-valley alluvial deposits contribute over 84% of total inflow, while direct recharge from precipitation (averaging 120 mm/year, 24.7% of annual rainfall) and riverbed leakage together account for only 11.4%. Recharge occurs predominantly from November to April, with no recharge from June to August. Under future scenarios, winter recharge may increase by up to 22.7%, while summer recharge could decline by up to 100%. Groundwater storage is projected to decrease by 7.3% to 58.3% compared to 2010–2020, indicating the aquifer’s vulnerability to prolonged dry periods. These findings emphasize the urgent need for adaptive water management strategies and long-term monitoring to ensure sustainable groundwater use under changing climate conditions. Full article
(This article belongs to the Special Issue Climate Change Uncertainties in Integrated Water Resources Management)
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20 pages, 27453 KiB  
Article
Natural and Anthropogenic Influence on the Physicochemical Characteristics of Spring Water: The Case Study of Medvednica Mountain (Central Croatia)
by Ivan Martinić and Ivan Čanjevac
Limnol. Rev. 2025, 25(3), 36; https://doi.org/10.3390/limnolrev25030036 - 1 Aug 2025
Viewed by 76
Abstract
During the period from 2020 to 2024, 900 springs were mapped on the southern slopes of Medvednica Mountain Nature Park. Physicochemical parameters (temperature, pH, and electrical conductivity) were measured at 701 of these springs using a portable multimeter, and results were analyzed in [...] Read more.
During the period from 2020 to 2024, 900 springs were mapped on the southern slopes of Medvednica Mountain Nature Park. Physicochemical parameters (temperature, pH, and electrical conductivity) were measured at 701 of these springs using a portable multimeter, and results were analyzed in relation to local lithology and human activities. This research provides the first results of this kind in this study area, aiming to expand the knowledge on local springs and to support the future protection and management of spring ecosystems. Springs on the Medvednica mountain showed substantial variation in measured parameters. The temperature ranged from 3.4 to 18.9 °C, reflecting local hydrological conditions, aquifer characteristics, and seasonal variability. Electrical conductivity (EC) ranged between 41 μS/cm and 2062 μS/cm, determined by both hydrogeological settings and anthropogenic impacts such as winter road salting. The pH values showed moderate variability, remaining mostly within neutral levels. These results emphasize the importance of continued monitoring and further research of Medvednica springs, in order to highlight their importance and to preserve their ecological and hydrological roles. Full article
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24 pages, 6020 KiB  
Article
Seasonal Patterns of Preterm Birth During the COVID-19 Pandemic: A Retrospective Cohort Study in Romania
by Paula Trif, Cristian Sava, Diana Mudura, Boris W. Kramer, Radu Galiș, Maria Livia Ognean, Alin Iuhas and Claudia Maria Jurca
Medicina 2025, 61(8), 1398; https://doi.org/10.3390/medicina61081398 - 1 Aug 2025
Viewed by 199
Abstract
Background and Objectives: Preterm birth and stillbirth are primary adverse pregnancy outcomes. Research during the COVID-19 pandemic revealed reductions in preterm birth in some countries, while stillbirth rates increased or remained unchanged. These findings suggest the presence of preventable risk factors associated with [...] Read more.
Background and Objectives: Preterm birth and stillbirth are primary adverse pregnancy outcomes. Research during the COVID-19 pandemic revealed reductions in preterm birth in some countries, while stillbirth rates increased or remained unchanged. These findings suggest the presence of preventable risk factors associated with changes in physical activity and lower exposure to community-acquired infections due to lockdown measures, altered social interaction patterns or reduced access to antenatal care. Assessing seasonal variation may offer insights into whether lifestyle changes during the COVID-19 lockdown period influenced preterm birth rates. Materials and Methods: This retrospective cohort study used data from the electronic medical records of Bihor and Sibiu counties. Preterm deliveries (<37 weeks) and stillbirths during the COVID-19 pandemic (2020 and 2021) were compared with the corresponding pre-pandemic (2018 and 2019) and post-pandemic (2022 and 2023) period. Preterm birth rates during summer and winter in the pre-pandemic, pandemic, and post-pandemic years were analyzed. A comparison with rates during strict lockdown was made. Results: Out of 52,021 newborn infants, 4473 were born preterm. Preterm birth rates remained stable across all three periods (p = 0.13), and no significant seasonal pattern was identified (p = 0.65). In contrast, stillbirth rates increased notably during the strict lockdown period, with the median incidence almost doubling compared to other periods (0.87%, p = 0.05), while remaining unchanged during the rest of the pandemic (p = 0.52). Conclusions: Our study found that preterm birth rates remained unaffected by the pandemic and lockdown periods, while stillbirths increased significantly during the strict lockdown. These findings highlight the importance of maintaining access to timely antenatal care during public health emergencies to prevent adverse perinatal outcomes. Full article
(This article belongs to the Special Issue Advances in Obstetrics and Maternal-Fetal Medicine)
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13 pages, 1189 KiB  
Article
Positive Effects of Reduced Tillage Practices on Earthworm Population Detected in the Early Transition Period
by Irena Bertoncelj, Anže Rovanšek and Robert Leskovšek
Agriculture 2025, 15(15), 1658; https://doi.org/10.3390/agriculture15151658 - 1 Aug 2025
Viewed by 186
Abstract
Tillage is a major factor influencing soil biological communities, particularly earthworms, which play a key role in soil structure and nutrient cycling. To address soil degradation, less-intensive tillage practices are increasingly being adopted globally and have shown positive effects on earthworm populations when [...] Read more.
Tillage is a major factor influencing soil biological communities, particularly earthworms, which play a key role in soil structure and nutrient cycling. To address soil degradation, less-intensive tillage practices are increasingly being adopted globally and have shown positive effects on earthworm populations when applied consistently over extended periods. However, understanding of the earthworm population dynamics in the period following the implementation of changes in tillage practices remains limited. This three-year field study (2021–2023) investigates earthworm populations during the early transition phase (4–6 years) following the conversion from conventional ploughing to conservation (<8 cm depth, with residue retention) and no-tillage systems in a temperate arable system in central Slovenia. Earthworms were sampled annually in early October from three adjacent fields, each following the same three-year crop rotation (maize—winter cereal + cover crop—soybeans), using a combination of hand-sorting and allyl isothiocyanate (AITC) extraction. Results showed that reduced tillage practices significantly increased both earthworm biomass and abundance compared to conventional ploughing. However, a significant interaction between tillage and year was observed, with a sharp decline in earthworm abundance and mass in 2022, likely driven by a combination of 2022 summer tillage prior to cover crop sowing and extreme drought conditions. Juvenile earthworms were especially affected, with their proportion decreasing from 62% to 34% in ploughed plots and from 63% to 26% in conservation tillage plots. Despite interannual fluctuations, no-till showed the lowest variability in earthworm population. Long-term monitoring is essential to disentangle management and environmental effects and to inform resilient soil management strategies. Full article
(This article belongs to the Section Agricultural Soils)
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25 pages, 2666 KiB  
Article
Hormonal Balance in Relation to Expression of Selected Genes Connected with Hormone Biosynthesis and Signalling—The Effect of Deacclimation Process in Oilseed Rape
by Magdalena Rys, Jan Bocianowski, Michał Dziurka, Barbara Jurczyk, Julia Stachurska, Piotr Waligórski and Anna Janeczko
Int. J. Mol. Sci. 2025, 26(15), 7408; https://doi.org/10.3390/ijms26157408 - 1 Aug 2025
Viewed by 144
Abstract
Global climate change is causing increasing fluctuations in winter temperatures, including episodes of warm conditions above 9 °C. Such events disrupt cold acclimation in plants and can induce deacclimation, reducing frost tolerance and altering, among other things, hormonal regulation. This study investigated hormonal [...] Read more.
Global climate change is causing increasing fluctuations in winter temperatures, including episodes of warm conditions above 9 °C. Such events disrupt cold acclimation in plants and can induce deacclimation, reducing frost tolerance and altering, among other things, hormonal regulation. This study investigated hormonal and molecular changes associated with cold acclimation and deacclimation in oilseed rape (Brassica napus L.) cultivars Kuga and Thure. Plants were grown under different conditions: non-acclimated (17 °C for three weeks), cold-acclimated (4 °C for three weeks), and deacclimated (16/9 °C day/night for one week). Detailed hormone analysis included auxins, gibberellins, cytokinins, stress-related hormones, and the expression of hormone-related genes (BnABF2, BnAOS, BnARF1, BnARR6, BnICS1, BnRGA, and BnWRKY57). Hormone concentrations in leaves changed dynamically in response to deacclimation with increased amounts of growth-promoting hormones and decreased amounts of stress hormones. Additionally, alterations in gene expression during deacclimation, such as in BnABF2 and BnICS1, may function as protective mechanisms to help maintain or regain frost tolerance during reacclimation when temperatures decline again after the warm period. These findings improve the understanding of hormonal and molecular responses involved in the deacclimation of oilseed rape. Full article
(This article belongs to the Special Issue Plant Hormone Signaling)
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24 pages, 6731 KiB  
Article
Combined Impacts of Acute Heat Stress on the Histology, Antioxidant Activity, Immunity, and Intestinal Microbiota of Wild Female Burbot (Lota Lota) in Winter: New Insights into Heat Sensitivity in Extremely Hardy Fish
by Cunhua Zhai, Yutao Li, Ruoyu Wang, Haoxiang Han, Ying Zhang and Bo Ma
Antioxidants 2025, 14(8), 947; https://doi.org/10.3390/antiox14080947 (registering DOI) - 31 Jul 2025
Viewed by 305
Abstract
Temperature fluctuations caused by climate change and global warming pose a threat to fish. The burbot (lota lota) population is particularly sensitive to increased water temperature, but the systematic impacts of high-temperature exposure on their liver and intestinal health remain unclear. [...] Read more.
Temperature fluctuations caused by climate change and global warming pose a threat to fish. The burbot (lota lota) population is particularly sensitive to increased water temperature, but the systematic impacts of high-temperature exposure on their liver and intestinal health remain unclear. In January of 2025, we collected wild adult burbot individuals from the Ussuri River (water temperature: about 2 °C), China. The burbot were exposed to 2 °C, 7 °C, 12 °C, 17 °C, and 22 °C environments for 96 h; then, the liver and intestinal contents were subsequently collected for histopathology observation, immunohistochemistry, biochemical index assessment, and transcriptome/16S rDNA sequencing analysis. There was obvious liver damage including hepatocyte necrosis, fat vacuoles, and cellular peripheral nuclei. Superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px) activities were elevated and subsequently decreased. Additionally, the malondialdehyde (MDA) level significantly increased with increasing temperature. These results indicate that 7 °C (heat stress temperature), 12 °C (tipping point for normal physiological metabolism status), 17 °C (tipping point for individual deaths), and 22 °C (thermal limit) are critical temperatures in terms of the physiological response of burbot during their breeding period. In the hepatic transcriptome profiling, 6538 differentially expressed genes (DEGs) were identified, while KEGG enrichment analysis showed that high-temperature stress could affect normal liver function by regulating energy metabolism, immune, and apoptosis-related pathways. Microbiomics also revealed that acute heat stress could change the intestinal microbe community structure. Additionally, correlation analysis suggested potential regulatory relationships between intestinal microbe taxa and immune/apoptosis-related DEGs in the liver. This study revealed the potential impact of environmental water temperature changes in cold habitats in winter on the physiological adaptability of burbot during the breeding period and provides new insights for the ecological protection of burbot in the context of global climate change and habitat warming. Full article
(This article belongs to the Special Issue Antioxidant Response in Aquatic Animals)
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26 pages, 3711 KiB  
Article
Probability Characteristics of High and Low Flows in Slovakia: A Comprehensive Hydrological Assessment
by Pavla Pekárová, Veronika Bačová Mitková and Dana Halmová
Hydrology 2025, 12(8), 199; https://doi.org/10.3390/hydrology12080199 - 31 Jul 2025
Viewed by 241
Abstract
Frequency analysis is essential for designing hydraulic structures and managing water resources, as it helps assess hydrological extremes. However, changes in river basins can impact their accuracy, complicating the link between discharge and return periods. This study aims to comprehensively assess the probability [...] Read more.
Frequency analysis is essential for designing hydraulic structures and managing water resources, as it helps assess hydrological extremes. However, changes in river basins can impact their accuracy, complicating the link between discharge and return periods. This study aims to comprehensively assess the probability characteristics of long-term M-day maximum/minimum discharges in the Carpathian region of Slovakia. We analyze the long-term data from 26 gauging stations covering 90 years of observation. Slovak rivers show considerable intra-annual variability, especially between the summer–autumn (SA) and winter–spring (WS) seasons. To allow consistent comparisons, we apply a uniform methodology to estimate T-year daily maximum and minimum specific discharges over durations of 1 and 7 days for both seasons. Our findings indicate that 1-day maximum specific discharges are generally higher during the SA season compared to the WS season. The 7-day minimum specific discharges are lower during the WS season compared to the SA season. Slovakia’s diverse orographic and climatic conditions cause significant spatial variability in extreme discharges. However, the estimated T-year 7-day minimum and 1-day maximum specific discharges, based on the mean specific discharge and the altitude of the water gauge, exhibit certain nonlinear dependences. These relationships could support the indirect estimation of T-year M-day discharges in regions with similar runoff characteristics. Full article
(This article belongs to the Section Water Resources and Risk Management)
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17 pages, 3966 KiB  
Article
Beyond the Detour: Modeling Traffic System Shocks After the Francis Scott Key Bridge Failure
by Daeyeol Chang, Niyeyesh Meimandi Nejad, Mansoureh Jeihani and Mansha Swami
Sustainability 2025, 17(15), 6916; https://doi.org/10.3390/su17156916 - 30 Jul 2025
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Abstract
This research examines the traffic disruptions resulting from the collapse of the Francis Scott Key Bridge in Baltimore, utilizing advanced econometric methods and real-time ClearGuide data. Employing Fixed Effects (FEs), Mixed Effects (MEs), Difference-in-Differences (DiDs), and stratified regression models, the study uniquely examines [...] Read more.
This research examines the traffic disruptions resulting from the collapse of the Francis Scott Key Bridge in Baltimore, utilizing advanced econometric methods and real-time ClearGuide data. Employing Fixed Effects (FEs), Mixed Effects (MEs), Difference-in-Differences (DiDs), and stratified regression models, the study uniquely examines the impacts of congestion across Immediate, Fall, and Winter periods, distinctly separating AM and PM peak patterns. Significant findings include severe PM peak congestion, up to four times greater than AM peak congestion, particularly on critical corridors such as the Harbor Tunnel Thruway northbound and MD-295 northbound. Initial route-level impacts were heterogeneous, gradually becoming uniform as the network adapted. The causal DiD analysis provides strong evidence that increased congestion is causally linked to proximity to the collapse. It is anticipated that incorporating the suggested framework will yield insightful information for stakeholders and decision-makers, such as targeted freight restriction, peak-hour dynamic pricing, corridor-specific signal adjustments, and investments in real-time traffic monitoring systems to strengthen transportation network resilience. Full article
(This article belongs to the Section Sustainable Transportation)
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