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17 pages, 7323 KiB  
Article
Line Laser 3D Measurement Method and Experiments of Gears
by Yanqiang Sun, Zhaoyao Shi, Bo Yu and Meichuan Li
Photonics 2025, 12(8), 782; https://doi.org/10.3390/photonics12080782 (registering DOI) - 4 Aug 2025
Abstract
Line laser measurement, as a typical method of laser triangulation, makes the acquisition of 3D tooth-surface data more accurate, efficient, and informative. Thus, a line laser 3D measurement model of gears is established, and a specialized polyhedral artifact with specific geometric features is [...] Read more.
Line laser measurement, as a typical method of laser triangulation, makes the acquisition of 3D tooth-surface data more accurate, efficient, and informative. Thus, a line laser 3D measurement model of gears is established, and a specialized polyhedral artifact with specific geometric features is invented to determine the pose parameters of the line laser sensor in measuring space. Based on this, a single-spindle gear-measuring instrument is developed and a series of experimental studies are conducted for gears with different module and flank directions in this instrument, including profile deviation, helix deviation, pitch deviation, topological deviation, etc. A comparative experiment with traditional contact measurement methods validates the correctness of the methods mentioned in this paper for the accurate evaluation of tested gears. In further research, the mining and utilization of big data obtained from the line laser 3D measurement of gears will be an important topic. Full article
(This article belongs to the Special Issue Advancements in Optical Metrology and Imaging)
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22 pages, 4262 KiB  
Article
Tribo-Dynamics of Dual-Star Planetary Gear Systems: Modeling, Analysis, and Experiments
by Jiayu Zheng, Yonggang Xiang, Changzhao Liu, Yixin Wang and Zonghai Mou
Sensors 2025, 25(15), 4709; https://doi.org/10.3390/s25154709 - 30 Jul 2025
Viewed by 215
Abstract
To address the unclear coupling mechanism between thermal elastohydrodynamic lubrication (TEHL) and dynamic behaviors in planetary gear systems, a novel tribo-dynamic model for dual-star planetary gears considering TEHL effects is proposed. In this model, a TEHL surrogate model is first established to determine [...] Read more.
To address the unclear coupling mechanism between thermal elastohydrodynamic lubrication (TEHL) and dynamic behaviors in planetary gear systems, a novel tribo-dynamic model for dual-star planetary gears considering TEHL effects is proposed. In this model, a TEHL surrogate model is first established to determine the oil film thickness and sliding friction force along the tooth meshing line. Subsequently, the dynamic model of the dual-star planetary gear transmission system is developed through coordinate transformations of the dual-star gear train. Finally, by integrating lubrication effects into both time-varying mesh stiffness and time-varying backlash, a tribo-dynamic model for the dual-star planetary gear transmission system is established. The study reveals that the lubricant film thickness is positively correlated with relative sliding velocity but negatively correlated with unit line load. Under high-speed conditions, a thickened oil film induces premature meshing contact, leading to meshing impacts. In contrast, under high-torque conditions, tooth deformation dominates meshing force fluctuations while lubrication influence diminishes. By establishing a test bench for the planetary gear transmission system, the obtained simulation conclusions are verified. This research provides theoretical and experimental support for the design of high-reliability planetary gear systems. Full article
(This article belongs to the Special Issue Feature Papers in Physical Sensors 2025)
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24 pages, 1686 KiB  
Review
Data-Driven Predictive Modeling for Investigating the Impact of Gear Manufacturing Parameters on Noise Levels in Electric Vehicle Drivetrains
by Krisztián Horváth
World Electr. Veh. J. 2025, 16(8), 426; https://doi.org/10.3390/wevj16080426 - 30 Jul 2025
Viewed by 234
Abstract
Reducing gear noise in electric vehicle (EV) drivetrains is crucial due to the absence of internal combustion engine noise, making even minor acoustic disturbances noticeable. Manufacturing parameters significantly influence gear-generated noise, yet traditional analytical methods often fail to predict these complex relationships accurately. [...] Read more.
Reducing gear noise in electric vehicle (EV) drivetrains is crucial due to the absence of internal combustion engine noise, making even minor acoustic disturbances noticeable. Manufacturing parameters significantly influence gear-generated noise, yet traditional analytical methods often fail to predict these complex relationships accurately. This research addresses this gap by introducing a data-driven approach using machine learning (ML) to predict gear noise levels from manufacturing and sensor-derived data. The presented methodology encompasses systematic data collection from various production stages—including soft and hard machining, heat treatment, honing, rolling tests, and end-of-line (EOL) acoustic measurements. Predictive models employing Random Forest, Gradient Boosting (XGBoost), and Neural Network algorithms were developed and compared to traditional statistical approaches. The analysis identified critical manufacturing parameters, such as surface waviness, profile errors, and tooth geometry deviations, significantly influencing noise generation. Advanced ML models, specifically Random Forest, XGBoost, and deep neural networks, demonstrated superior prediction accuracy, providing early-stage identification of gear units likely to exceed acceptable noise thresholds. Integrating these data-driven models into manufacturing processes enables early detection of potential noise issues, reduces quality assurance costs, and supports sustainable manufacturing by minimizing prototype production and resource consumption. This research enhances the understanding of gear noise formation and offers practical solutions for real-time quality assurance. Full article
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14 pages, 6112 KiB  
Article
Polytetrafluoroethylene Isolation of the Periodontal Sulcus for Cementation of Full Veneer Restorations Using a Biologically Oriented Preparation Technique (BOPT): An In Vitro Study
by José Félix Mañes, Federica Tripodi, Jorge Alonso Pérez-Barquero, Blanca Serra-Pastor, Ana Roig-Vanaclocha, Jesús Maneiro-Lojo, Ignazio Loi and Rubén Agustín-Panadero
J. Clin. Med. 2025, 14(15), 5305; https://doi.org/10.3390/jcm14155305 - 27 Jul 2025
Viewed by 326
Abstract
Background: Prosthetic cementation using the biologically oriented preparation technique (BOPT) presents challenges in removing excess cement from the gingival sulcus, due to the absence of a finishing line and the impossibility of using absolute isolation with a rubber dam. This study aimed to [...] Read more.
Background: Prosthetic cementation using the biologically oriented preparation technique (BOPT) presents challenges in removing excess cement from the gingival sulcus, due to the absence of a finishing line and the impossibility of using absolute isolation with a rubber dam. This study aimed to evaluate the effectiveness of relative isolation using polytetrafluoroethylene (PTFE) tape in reducing cement retention during BOPT cementation. Methods: Fifteen 3D-printed resin models were created from an intraoral scan of a patient restored with BOPT in both upper central incisors. Each model included removable gingiva. Splinted polymethylmethacrylate (PMMA) provisional crowns were fabricated and cemented with temporary cement. One central incisor was isolated with PTFE (0.1 mm or 0.2 mm), while the contralateral tooth was left unisolated as a control. After debonding, digital scanning and volumetric analysis using root mean square (RMS) deviation were performed to quantify retained cement. Paired t-tests were applied to compare groups. Results: The mean RMS for the PTFE group was 0.1248 ± 0.0519 mm, compared to 0.1973 ± 0.0361 mm in the non-isolated group (p < 0.001). No significant difference was found between PTFE thicknesses of 0.1 mm and 0.2 mm (p = 0.388). Conclusions: PTFE tape is effective for relative isolation when rubber dam placement is not feasible in BOPT restorations. Further clinical studies are recommended to confirm these findings in vivo. Full article
(This article belongs to the Special Issue Clinical Updates on Prosthodontics)
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32 pages, 21606 KiB  
Article
Calculation Method and Experimental Investigation of Root Bending Stress in Line Contact Spiral Bevel Gear Pairs
by Shiyu Zuo, Yuehai Sun, Liang Chen, Simin Li and Mingyang Wang
Machines 2025, 13(8), 632; https://doi.org/10.3390/machines13080632 - 22 Jul 2025
Viewed by 287
Abstract
Compared to spiral bevel gear drives with localized conjugation, line contact spiral bevel gears possess a significantly larger meshing area, theoretically achieving full tooth surface contact and substantially enhancing load capacity. To accurately support the root strength calculation and parameter design of line [...] Read more.
Compared to spiral bevel gear drives with localized conjugation, line contact spiral bevel gears possess a significantly larger meshing area, theoretically achieving full tooth surface contact and substantially enhancing load capacity. To accurately support the root strength calculation and parameter design of line contact spiral bevel gear drives, this paper presents a theoretical analysis and experimental study of the root bending stress of gear pairs. First, based on the analysis of the meshing characteristics of line contact spiral bevel gear pairs, the load distribution along the contact lines is investigated. Using the slicing method, the load distribution characteristics along the contact line are obtained, and the load sharing among multiple tooth pairs during meshing is further studied. Then, by applying a cantilever beam bending stress model, the root bending stress on such a gear drive is calculated. A root bending moment distribution model is proposed based on the characteristics of the line load distribution previously obtained, from which a formula for calculating root bending stress is derived. Finally, static-condition experiments are conducted to test the root bending stress. The accuracy of the proposed calculation method is verified through experimental testing and finite element analysis. The results of this study provide a foundation for designing lightweight and high-power-density spiral bevel gear drives. Full article
(This article belongs to the Section Machine Design and Theory)
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27 pages, 8932 KiB  
Article
Strategies for Mitigating Runout Interference in Torsional Vibration Measurement of Diesel Engine Crankshafts
by Chenghao Qi and Zhongxu Tian
Appl. Sci. 2025, 15(13), 7355; https://doi.org/10.3390/app15137355 - 30 Jun 2025
Viewed by 210
Abstract
The precise measurement of crankshaft torsional vibration is critical for diesel engine reliability, yet it is often compromised by systematic errors from toothed disc runout. To address this challenge, this paper elucidates the dual mechanism of these errors, which manifest as micro-level voltage [...] Read more.
The precise measurement of crankshaft torsional vibration is critical for diesel engine reliability, yet it is often compromised by systematic errors from toothed disc runout. To address this challenge, this paper elucidates the dual mechanism of these errors, which manifest as micro-level voltage fluctuations in signal and macro-level time-domain deviations. Based on this understanding, a composite compensation method is proposed. First, a dual-line approximation method is presented for preprocessing the raw sensor signals, aiming to eliminate the distortion in rotational speed calculations caused by anomalous voltages. Second, a synchronous sampling scheme based on the differential measurement principle is developed. This scheme utilizes a symmetrically arranged dual-sensor structure to suppress runout errors and is combined with a time-domain feature reconstruction technique to restore the true rotational speed signal. Validation on a custom-built universal joint torsional vibration test rig demonstrates that the proposed method can effectively eliminate systematic deviations arising from toothed disc runout, thereby significantly enhancing the accuracy of torsional vibration measurements. The measurement method presented in this paper offers a valuable reference for the high-precision measurement of engine torsional vibration characteristics. Full article
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17 pages, 3627 KiB  
Article
Stress Distribution on Endodontically Treated Anterior Teeth Restored via Different Ceramic Materials with Varying Post Lengths Versus Endocrown—A 3D Finite Element Analysis
by Mai Soliman, Nawaf Almutairi, Ali Alenezi, Raya Alenezi, Amal Abdallah A. Abo-Elmagd and Manal M. Abdelhafeez
J. Funct. Biomater. 2025, 16(6), 221; https://doi.org/10.3390/jfb16060221 - 12 Jun 2025
Viewed by 995
Abstract
Objective: This study aims to evaluate the stress distribution on endodontically treated anterior teeth restored using different restorative materials and different post lengths versus endocrowns employing finite element analysis (FEA). Methods: An extracted human central incisor tooth with a fully formed apex was [...] Read more.
Objective: This study aims to evaluate the stress distribution on endodontically treated anterior teeth restored using different restorative materials and different post lengths versus endocrowns employing finite element analysis (FEA). Methods: An extracted human central incisor tooth with a fully formed apex was scanned using high-resolution cone beam computed tomography (CBCT) to generate 3D finite element models. Six models of restorations of badly destructed central incisor were grouped according to the type of ceramic material and post length versus endocrown restorations. Group V-L: Vita Enamic, long post (10 mm intra-radicular), Group C-L: Celtra Duo, long post (10 mm intra-radicular), Group V-Sh: Vita Enamic, short post (3 mm intra-radicular), Group C-Sh: Celtra Duo, short post (3 mm intra-radicular), Group V-E: Vita Enamic endocrown (3 mm intra-radicular), and Group C-E: Celtra Duo endocrown (3 mm intra-radicular). A static load of 200 N was applied to the palatal surface at a 45 degree angle to the tooth’s long axis. The maximum equivalent von Mises stress and maximum principal stress were analyzed at four locations: the finish line, coronal third of the root (12 mm from the apex), middle third of the root (8 mm from the apex), and apical third of the root (4 mm from the apex). Results: Group C-L exhibited the highest maximum VM stress and PS at the finish line, in addition to the highest maximum VM stress and PS at the root apical third, while group C-Sh reported the least maximum VM stress at the root apical third among the groups. All Celtra Duo groups reported higher maximum VM stress than the corresponding groups of Vita Enamic at the finish line and root coronal thirds. However, at the root middle and apical thirds, both materials recorded similar stresses. Conclusions: Short posts and Vita Enamic endocrowns showed minimal stress, especially at the finish line, while long posts increased stress and fracture risk. The findings support conservative restorations without posts, although clinical validation is needed to confirm their long-term effectiveness and safety. Full article
(This article belongs to the Special Issue Advances in Restorative Dentistry Materials)
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10 pages, 2445 KiB  
Case Report
Guided Endodontics for a Tooth with Root Fracture: A Case Report
by Monika Kuczmaja and Agata Żółtowska
J. Clin. Med. 2025, 14(12), 4079; https://doi.org/10.3390/jcm14124079 - 9 Jun 2025
Viewed by 705
Abstract
Background: A 19-year-old female patient reported to the Department of Conservative Dentistry, Medical University of Gdańsk, with pain in the left lateral incisor. During the attempt to perform root canal treatment on tooth 22, we encountered difficulties in locating the canal opening, which [...] Read more.
Background: A 19-year-old female patient reported to the Department of Conservative Dentistry, Medical University of Gdańsk, with pain in the left lateral incisor. During the attempt to perform root canal treatment on tooth 22, we encountered difficulties in locating the canal opening, which resulted in treatment failure. Radiographic examinations revealed Pulp Canal Obliteration and a root fracture with a double fracture line, resulting in two root fragments. The prognosis for this tooth was poor, with extraction being the most likely outcome. Methods: To provide effective therapy, a 3D-printed endodontic guide was utilized, allowing for more precise localization of the canal in a tooth with a calcified root canal and complex fracture morphology. An intraoral scan and cone-beam computed tomography (CBCT) were performed, followed by the design of the guide. This facilitated accurate planning of the entry path for endodontic instruments, promoting minimally invasive treatment and reducing the loss of tooth structure. Results: Through navigated endodontics, the treatment became more predictable, and the risk of iatrogenic complications was minimized, significantly improving the prognosis of the therapy. Clinical and radiological examinations conducted after 12 months demonstrated successful treatment and preservation of the tooth in the oral cavity. Conclusions: The obtained results suggest that the use of guided endodontics can improve outcomes in cases with pulp canal obliteration and complex fractures, offering a minimally invasive and predictable approach. Full article
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20 pages, 6458 KiB  
Article
Research on Curvature Interference Characteristics of Conical Surface Enveloping Cylindrical Worm–Face Worm Gear Drive
by Shibo Mu, Xingwei Sun, Zhixu Dong, Heran Yang, Yin Liu, Weifeng Zhang, Sheng Qu, Hongxun Zhao and Yaping Zhao
Appl. Sci. 2025, 15(11), 6298; https://doi.org/10.3390/app15116298 - 3 Jun 2025
Viewed by 445
Abstract
This study proposes the use of Physics-Informed Neural Networks (PINNs) to further advance the curvature interference analysis method. The nonlinear equation system encountered in determining the curvature interference limit line is embedded into the PINN loss function, thereby enabling the solution of high-dimensional, [...] Read more.
This study proposes the use of Physics-Informed Neural Networks (PINNs) to further advance the curvature interference analysis method. The nonlinear equation system encountered in determining the curvature interference limit line is embedded into the PINN loss function, thereby enabling the solution of high-dimensional, nonlinear equations. Computational results demonstrate that the PINN model achieves a solution accuracy on the order of 10−13 when solving multidimensional nonlinear systems, which is comparable to the classical Fsolve algorithm. The curvature interference analysis reveals the presence of two curvature interference boundary lines, although they rarely extend to the worm gear tooth surface. A study on the influence of design parameters on the interference boundaries indicates that the axial installation distance has the greatest impact. Inadequate axial spacing causes the interference limit line to shift toward the inner end of the worm gear, significantly increasing the risk of interference in that region. The proposed curvature interference analysis method based on PINNs can be extended to other types of gear drives. It also lays the foundation for future work on establishing both forward and inverse mappings between design parameters and curvature interference using PINNs. Full article
(This article belongs to the Section Mechanical Engineering)
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18 pages, 9989 KiB  
Article
Study on Vibration Characteristics and Transmission Path of Mountain Rack Trains Based on the OPTA Method
by Liangzhao Qi, Xingqiao Deng, Liyuan Zeng, Chenglong Dong, Yixin Xu, Shisong Wang and Yucheng Liu
Machines 2025, 13(6), 482; https://doi.org/10.3390/machines13060482 - 3 Jun 2025
Viewed by 360
Abstract
The Dujiangyan–Siguniangshan mountain rack railway project is China’s first mountain rail transit. Most of its lines are located in mountainous areas and close to natural ecological protection areas, which have strict restrictions on the vibration and noise of train operation. At the same [...] Read more.
The Dujiangyan–Siguniangshan mountain rack railway project is China’s first mountain rail transit. Most of its lines are located in mountainous areas and close to natural ecological protection areas, which have strict restrictions on the vibration and noise of train operation. At the same time, the vibration of mountain rack railway trains is also an important factor affecting the safety and riding comfort of trains. However, due to the multi-source vibration of gear teeth, wheels, rails, and suspensions, it is difficult to clearly define the vibration characteristics and vibration transmission path of the train, which has a serious impact on its vibration noise suppression and optimization. To this end, this study proposed a set of evaluation methods for the vibration characteristics and transfer paths of mountain rack trains based on a combination of dynamics and operational transfer path analysis (OTPA). Considering the interaction between the dynamic behaviors of the primary and secondary suspensions, the gear tooth contact behavior, the wheel–rail contact behavior and the dynamic behaviors of the track system, a dynamic model of a mountain rack train based on the finite element method was established, and the effectiveness of the model was verified through field experiments. On this basis, the OTPA method was used to establish a vibration transfer path model between the secondary suspension and the center of mass of the car body, and it was used to analyze the vibration mechanism and transfer path of the train body at the rated speed (20 km/h) and the limited speed (30 km/h). This study is of great significance for suppressing the vibration noise of mountain rack trains, reducing the impact on the ecological environment and improving ride comfort. Full article
(This article belongs to the Section Vehicle Engineering)
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7 pages, 697 KiB  
Proceeding Paper
Construction of Fully Automated Key Production Line
by Guo-Cheng Lee, Yi-Hsuan Chiu and Kuang-Chyi Lee
Eng. Proc. 2025, 92(1), 83; https://doi.org/10.3390/engproc2025092083 - 27 May 2025
Cited by 1 | Viewed by 263
Abstract
We developed a fully automated key production line for smart manufacturing technologies based on the Internet of Things (IoT) and automatic optical inspection (AOI) to enable efficient and consistent production. The production line consists of seven processing stations: raw materials uploading, groove milling, [...] Read more.
We developed a fully automated key production line for smart manufacturing technologies based on the Internet of Things (IoT) and automatic optical inspection (AOI) to enable efficient and consistent production. The production line consists of seven processing stations: raw materials uploading, groove milling, laser marking, key tooth cutting, deburring, defects inspection, and a discharge station. IoT technology enables real-time monitoring and data transmission through a visual panel that displays the operational status of each station and provides immediate alerts in case of abnormalities for quick intervention. The defects inspection station ensures comprehensive quality checks, automatically stops the production line for detected defects, and prevents defective products from proceeding to subsequent stages. Chronological data are used to support predictive maintenance, production parameter optimization, and energy efficiency improvements. Overall, the system effectively integrates automation, real-time monitoring, and quality control to ensure stable production and high product quality. Full article
(This article belongs to the Proceedings of 2024 IEEE 6th Eurasia Conference on IoT, Communication and Engineering)
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22 pages, 5726 KiB  
Article
Simulation Prediction and Experimental Research on Surface Morphology of Ball Head Milling Processing
by Youzheng Cui, Xinmiao Li, Minli Zheng, Haijing Mu, Chengxin Liu, Dongyang Wang, Bingyang Yan, Qingwei Li, Hui Jiang, Fengjuan Wang and Qingming Hu
Materials 2025, 18(10), 2355; https://doi.org/10.3390/ma18102355 - 19 May 2025
Viewed by 464
Abstract
With the aim of improving the machined surface quality of die steel, this paper takes Cr12MoV quenched die steel as the research object and proposes a ball head milling surface morphology prediction model that comprehensively considers influencing factors, including tool vibration, eccentricity, as [...] Read more.
With the aim of improving the machined surface quality of die steel, this paper takes Cr12MoV quenched die steel as the research object and proposes a ball head milling surface morphology prediction model that comprehensively considers influencing factors, including tool vibration, eccentricity, as well as deformation. By setting key parameters, such as line spacing, feed per tooth, cutting depth, and phase difference, the system analyzed the influence of each parameter on the residual height and surface roughness of the machined surface. High-speed milling experiments were conducted, and the surface morphology of the samples was observed and measured under a microscope. The simulation results show good agreement with the experimental data, with errors within 7%~15%, proving the accuracy of the model. This study can provide theoretical support and methodological guidance for surface quality control and processing parameter optimization in complex mold surface machining. Full article
(This article belongs to the Topic Novel Cementitious Materials)
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14 pages, 641 KiB  
Review
Genetic Aspects of Tooth Agenesis
by Clarissa Modafferi, Ilaria Tucci, Francesco Maria Bogliardi, Elena Gimondo, Pietro Chiurazzi, Elisabetta Tabolacci and Cristina Grippaudo
Genes 2025, 16(5), 582; https://doi.org/10.3390/genes16050582 - 15 May 2025
Viewed by 1031
Abstract
Tooth agenesis is among the most prevalent congenital anomalies affecting human dentition, characterized by the developmental absence of one or more teeth. This condition may be present in either syndromic or non-syndromic forms, with significant implications for oral function, aesthetics, and craniofacial development. [...] Read more.
Tooth agenesis is among the most prevalent congenital anomalies affecting human dentition, characterized by the developmental absence of one or more teeth. This condition may be present in either syndromic or non-syndromic forms, with significant implications for oral function, aesthetics, and craniofacial development. This narrative review aims to provide a comprehensive overview of tooth agenesis, defining its classification, genetic underpinnings, epidemiological aspects, phenotypic features, and therapeutic approaches. Recent advances in genetic research have identified numerous causative genes, notably EDA, MSX1, WNT10A, and PAX9, each associated with specific patterns of missing teeth and involved in isolated and/or syndromic forms. Additionally, genes such as TSPEAR, LRP6, PITX2, and GREM2 contribute to varying degrees of severity and tooth distribution, often blurring the lines between syndromic and isolated cases. The genotype-phenotype correlations underscore the complexity of the underlying molecular pathways involved in odontogenesis. From a therapeutic perspective, the management of tooth agenesis requires a multidisciplinary approach, often involving orthodontic, prosthetic, and surgical interventions tailored to the severity of tooth loss and patient age. Early diagnosis represents a crucial role in treatment planning, facilitating timely intervention during growth and enhancing long-term outcomes. In conclusion, tooth agenesis remains a complex clinical condition with a strong genetic basis. A patient-centered and interdisciplinary strategy is essential to address both functional and psychosocial needs. Full article
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15 pages, 3214 KiB  
Article
Dimensional Accuracy of Regular- and Fast-Setting Vinyl Polysiloxane Impressions Using Customized Metal and Plastic Trays—An In Vitro Study
by Moritz Waldecker, Karla Jetter, Stefan Rues, Peter Rammelsberg and Andreas Zenthöfer
Materials 2025, 18(9), 2164; https://doi.org/10.3390/ma18092164 - 7 May 2025
Viewed by 559
Abstract
The aim of this study was to compare the dimensional accuracy of vinyl polysiloxane impressions differing in terms of curing time (regular-setting (RS) or fast-setting (FS)) in combination with different tray materials (metal (M) and plastic (P)). A typodont reference model simulated a [...] Read more.
The aim of this study was to compare the dimensional accuracy of vinyl polysiloxane impressions differing in terms of curing time (regular-setting (RS) or fast-setting (FS)) in combination with different tray materials (metal (M) and plastic (P)). A typodont reference model simulated a partially edentulous maxilla. Reference points were given by center points of either precision balls welded to specific teeth or finishing-line centers of prepared teeth. These reference points enabled the detection of dimensional deviations between the digitized reference and the scans of the models achieved from the study impressions. Twenty impressions were made for each of the following four test groups: RS-M, RS-P, FS-M and FS-P. Global scan data accuracy was measured by distance and tooth axis deviations from the reference, while local accuracy was determined based on the trueness and precision of the abutment tooth surfaces. Statistical analysis was conducted using ANOVA accompanied by pairwise Tukey post hoc tests (α = 0.05). Most of the distances tended to be underestimated. Global accuracy was favorable; even for long distances, the mean absolute distance deviations were < 100 µm. Local accuracy was excellent for all test groups, with trueness ≤ 11 µm and precision ≤ 9 µm. Within the limitations of this study, all impression and tray materials were suitable to fabricate models with clinically acceptable accuracy. Full article
(This article belongs to the Special Issue Advanced Biomaterials for Dental Applications (2nd Edition))
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13 pages, 2594 KiB  
Article
Harnessing Nanopore Sequencing to Investigate the Epigenomic Landscape in Molar Incisor Hypomineralization—A Pilot Study
by Silvia Salatino, Piotr Cuber, Wojciech Tynior, Carla Gustave, Dorota Hudy, Yuen-Ting Chan, Agnieszka Raczkowska-Siostrzonek, Raju Misra, Dagmara Aleksandrowicz, Dariusz Nałęcz and Joanna Katarzyna Strzelczyk
Int. J. Mol. Sci. 2025, 26(7), 3401; https://doi.org/10.3390/ijms26073401 - 5 Apr 2025
Viewed by 827
Abstract
Molar incisor hypomineralization (MIH) is a dental condition that affects the enamel of permanent molars and/or incisors, often leading to tooth decay. Although several etiological hypotheses have come forward, including prenatal medical problems and postnatal illness, the pathogenesis of MIH is yet unclear. [...] Read more.
Molar incisor hypomineralization (MIH) is a dental condition that affects the enamel of permanent molars and/or incisors, often leading to tooth decay. Although several etiological hypotheses have come forward, including prenatal medical problems and postnatal illness, the pathogenesis of MIH is yet unclear. Aimed at exploring the epigenomic landscape of this dental condition, we collected dental tissue from a MIH-affected child and an age-matched control patient and investigated their DNA methylation status through an in-depth analysis of nanopore long-read sequencing data. We identified 780,141 CpGs with significantly different methylation levels between the samples; intriguingly, the density of these dinucleotides was higher in the regions containing genes involved in dental morphogenesis and inflammatory processes leading to periodontitis. Further examination of 54 genes associated with MIH or hypomineralized second primary molar disorders revealed very distinct methylation of intragenic transposable elements (SINEs, LINEs, and LTRs), while functional profiling analysis of 571 differentially methylated regions genome-wide uncovered significant enrichment processes including ameloblasts differentiation and calcium ion binding, as well as SP1 and other zinc finger transcription factors. Taken together, our findings suggest that DNA methylation could play a role in the pathogenesis of MIH and represent a stepping stone towards a comprehensive understanding of this multifactorial disorder. Full article
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