Search Results (17)

Seasonal variability significantly influences the fate and transport of antibiotics (Abs) in wastewater stabilization ponds by affecting their concentration, degradation kinetics, sorption behavior, and ecological interactions. This study investigated the influence of seasonal variability for a large number of Ab classes—eleven sulfonamides (SAs), eight fluoroquinolones (FQs), five macrolides (MLs), one diaminopyrimidine (DIA), two tetracyclines (TETs), two lincosamides (LICs), and three phenicols (Phens)—on their fate and transport in an artificial stabilization pond system (SPS) receiving treated WWTP effluent. Two sampling campaigns were conducted during China’s long-lasting seasons (summer and winter). The detection frequency for sulfamethoxazole (SMX), sulfapyridine (SPY), and ofloxacin (OFX) was 100%, for sulfamethazine (SMZ) 63.3%, and for clindamycin (CLN) 83.3% in both seasons. The detection frequency for the other Abs was equal or below 50% in both seasons. In addition, the maximum concentration of SMX, SMZ, SPY, OFX, and CLN in summer was 10.51, 19.37, 6.93, 22, and 4.04 ng/L, respectively, and 4.27, 0.14, 3.15, 9.29, and 8.78, respectively, in winter). The rest of the Abs were either detected in summer or winter. It was observed that environmental fluctuations (such as temperature, precipitation, SPS flow patterns, light intensity), differences in antibiotic use and consumption between seasons, and differences in physicochemical properties of the Abs were the main factors influencing their fate and transport within the SPS. The potential environmental risks of Abs detected in the SPS were assessed using the risk quotient (RQ) approach. Typically, RQs in summer were remarkably higher than in winter. Norfloxacin and chlortetracycline posed a medium risk in summer; however, ofloxacin posed a medium risk in winter and a high risk in summer. Therefore, management strategies should consider the dynamic nature of antibiotic contamination, accounting for seasonal influences on fate and transport within the studied SPS and maybe for other wastewater stabilization ponds by adjusting operational practices, optimizing treatment processes, and implementing source control measures to mitigate the environmental impacts of seasonal antibiotic variability. Full article

A total of 34 antibiotics from five major classes of antibiotics, including macrolides, sulfonamides, quinolones, tetracyclines and chloramphenicol, were considered as contaminants, considering the Yellow River Estuary as the study area. The distribution, sources and ecological risks of typical antibiotics in the Yellow River Estuary were investigated using an optimized solid-phase extraction pre-treatment and an Agilent 6410B tandem triple-quadrupole liquid chromatography–mass spectrometer for antibiotic detection. The results show that antibiotics were widely present in the water bodies of the Yellow River Estuary, with 14 antibiotics detected to varying degrees, including a high detection rate for lincomycin hydrochloride. Farming wastewater and domestic sewage were the primary sources of antibiotics in the Yellow River Estuary. The distribution characteristics of antibiotics in the study area were linked to the development of farming and social activities. The ecological risk evaluation of 14 antibiotics in the Yellow River Estuary watershed showed that clarithromycin and doxycycline hydrochloride were present at medium-risk levels, and lincomycin hydrochloride, sulfamethoxazole, methomyl, oxifloxacin, enrofloxacin, sulfadiazine, roxithromycin, sulfapyridine, sulfadiazine and ciprofloxacin were present at low-risk levels in the samples collected from water bodies of the Yellow River Estuary. This study provides novel, beneficial information for the assessment of the ecological risk presented by antibiotics in the Yellow River Estuary water bodies and provides a scientific basis for future antibiotic pollution control in the Yellow River Basin. Full article

Antibiotics have been used to control the aquatic environment in both therapeutic and prophylactic ways. Antibiotics are particularly difficult to extract due to their strong interactions with biological matrices. In this study, UPLC-MS/MS method was developed and validated for quantitative confirmatory analysis of multi-class antibiotics residues in fish and shrimp. Fourteen antibiotics belonging to sulphonamides, β-lactams, quinolones, sulfones and macrolides were determined within one chromatographic run. The samples were suspended in 0.1 M HCl, and the analytes were extracted into ethyl acetate. The extracts were defatted with cyclohexane. The limits of quantification (LOQ) ranged from 0.24 to 1.32 µg kg⁻¹ for fish and 0.42–1.62 µg kg⁻¹ for shrimp samples. The recoveries ranged from 75 to 105%. The method was applied to the analysis of farmed freshwater Tilapia fish (Oreochromis niloticus) and shrimp (Penaeus monodon) collected in Sri Lanka. Sulfacetamide (4.31 ± 0.70 µg kg⁻¹) and sulfamethoxypyridazine (0.75 ± 0.15 µg kg⁻¹) were detected in the fish, and sulfapyridine (0.21–0.56 µg kg⁻¹) and sulfadoxine (0.35–1.44 µg kg⁻¹) were detected in the shrimp samples. The concentrations complied with the EU regulation limits for veterinary drug residues in seafood and did not pose a risk in terms of food safety. Full article

Highly efficient visible-light-driven heterogeneous photocatalyst Ag₃PO₄/g-C₃N₄ with different weight ratios from Ag₃PO₄ to g-C₃N₄ were synthesized by a facile in situ hydrothermal method and characterized by X-ray diffraction (XRD), scanning electron microscopy (SEM), Fourier transform infrared spectrometry (FTIR), photoluminescence spectra (PL), UV–vis diffuse reflectance spectra (UV-Vis), and electrochemical impedance spectra (EIS). Under visible light irradiation, Ag₃PO₄/g-C₃N₄ showed very excellent photocatalytic activity for sulfapyridine (SP) which is one of the widely used sulfonamide antibiotics. When the ratio from Ag₃PO₄ to g-C₃N₄ was 1:2, the degradation rate of SP at 120 min was found to be 94.1%, which was superior to that of pure Ag₃PO₄ and pure g-C₃N₄. Based on the experimental results, the possible enhanced photocatalytic mechanism of Ag₃PO₄/g-C₃N₄ was proposed. Full article

Although 5-Aminosalicylate (5-ASA) has been shown to act on the local mucosa, when 5-ASA is orally administered, most of it is absorbed in the upper gastrointestinal tract and does not reach the large intestine, where lesions are present. Therefore, different drug delivery systems have been developed for each oral 5-ASA formulation. Currently, the oral 5-ASA formulation approved in Japan is salazosulfapyridine (SALAZOPYRIN^®; Pfizer Japan Inc.: Tokyo, Japan), in which 5-ASA and sulfapyridine are azo-bonded. In addition, there are several 5-ASA release formulations, including ASACOL^®; ZERIA Pharmaceutical Co., Ltd.: Tokyo, Japan (delayed release formulation dependent on pH), PENTASA^®; KYORIN Pharmaceutical Co., Ltd.: Tokyo, Japan (delayed release formulation dependent on time), and LIALDA^®; MOCHIDA Pharmaceutical Co., Ltd.: Tokyo, Japan (delayed release formulation dependent on pH and time). Adverse events may occur because of differences in the drug delivery systems of these products. In this study, we focused on the adverse events of different 5-ASA formulations and investigated differences in the detection of safety signals for each 5-ASA formulation using disproportionality analysis. There were 15 adverse events detected only with SALAZOPYRIN^®. On the other hand, ASACOL^®, PENTASA^®, and LIALDA^® have different drug delivery systems. Although the detected signal intensities varied, the detected adverse events were not significantly different. These findings provide important insights, which should be considered by physicians during treatment selection and drug manufacturers during drug development. Full article

In this study, sulfapyridine (SPY), an antibiotic that is less commonly treated by membrane filtration techniques but is frequently detected in the aqueous environment and at higher concentrations than other detected antibiotics, was selected for investigation. A composite ultrafiltration membrane for the removal of sulfapyridine (SPY) antibiotics from water was fabricated using polyvinylidene fluoride (PVDF), polyvinylpyrrolidone (PVP), and carboxyl-functionalized graphene oxide (CFGO) as additives. The changes in retention rate and pure water flux of sulfapyridine by the composite ultrafiltration membrane were investigated by changing the ratios of the prepared ultrafiltration membrane materials under the conditions of low-pressure operation to explore the optimal experimental conditions. The results showed that the addition of PVP and CFGO significantly increased the number of membrane pores and their pore size. The addition of CFGO in the membrane significantly improved the hydrophilicity of the membrane. The contact angle decreased from 83.7 to 31.6°. Compared to ordinary PVDF ultrafiltration membranes, the membrane’s pure water flux increased nearly three times to 2612.95 L/(m²·h). The removal rate of SPY was 56.26% under the optimal conditions. When the composite ultrafiltration membrane was combined with activated carbon, the removal rate of SPY was 92.67%, which was nine times higher than that of activated carbon alone. At this time, the flux of the composite membrane was 2610.23 L/(m²·h). This study proposes a simple, efficient, and low production cost solution for the removal of sulfapyridine from water. Full article

With the extensive use of antibiotics, antibiotics, and antibiotic resistance genes (ARGs), contamination in the water environment is becoming severe in recent years. This study examined the distribution characteristics of sulfonamide antibiotics and resistance genes in the urban section of the Yitong River in autumn and winter. In addition, the correlation between them and water quality parameters was analyzed using Pearson correlation analysis. The surface water from the Yitong River was sampled in September and November for this experiment. The results of 32 samples showed that seven sulfonamides were detected in the surface water and sediments of this reach, and the concentrations were generally at the levels of ng/L and ng/g. The total concentration range was 11–161 ng/L and ND-85.7 ng/g. The concentrations of different antibiotics were similar in autumn and winter, and the concentration of sulfamethoxazole (SMX) was higher than that of other antibiotics. The results of the Risk Quotients (RQs) showed that SMX and sulfadiazine (SDZ) had moderate acute risk to the corresponding sensitive species in river water, sulfapyridine (SPD) and sulfisoxazole (SIZ) had low acute risk, while the rest had no risk. The total bacterial abundance in surface water and sediment was in the range of 10⁴–10⁵ copies/mL and 10⁸–10¹¹ copies/g, respectively. The detection rates of three sulfonamide resistance genes were 100%; the relative abundance was in the range of 10⁻³–10⁻¹ copies/16S rRNA, and sul1 was the primary resistance gene. The results of correlation analysis showed that there was a significant positive correlation between sulfamethazine (SMZ), sulfathiazole (STZ), and SIZ and water quality indexes such as total nitrogen (TN) and total phosphorus (TP), ammonia nitrogen (NH₃-N) and chlorophyll a (Chl-a). The river‘s change of water quality environment could significantly affect the antibiotics’ spatial distribution characteristics and content. The higher the level of nutrients, the higher the concentration of antibiotics. The abundance of sulfa resistance genes was positively correlated with the concentration of sulfa antibiotics. Frequent human activities can increase antibiotic concentration, leading to the production of more resistance genes induced by antibiotics, but the main reason for the formation of resistance genes was not apparent. Therefore, exploring the occurrence and pollution characteristics of antibiotics and ARGs in the environment of the Yitong River Basin in Changchun City and analyzing their sources, transmission, and ecological risks in the environment provided an essential scientific basis for pollution prevention and ecological protection of urban rivers in northern China. Full article

Pharmaceuticals and personal care products (PPCPs) were investigated in five wastewater treatment plants (WWTPs), groundwater, irrigated soils, and plants in Amman and Al-Balqa governorates in Jordan. PPCPs were extracted from water samples by solid-phase extraction (SPE) and analyzed by high-performance liquid chromatography coupled with tandem mass spectrometry (HPLC–MS/MS). Carbamazepine, ciprofloxacin, ceftiofur, diclofenac, erythromycin, lincomycin, ofloxacin, pyrimthamine, spiramycin, sulfamethoxazole, sulfapyridine, testosterone, trimethoprim, and thiamphenicol were detected in all raw wastewaters in μg/L, whereas 45 PPCPs were below the detection limits (<0.02 μg/L) in all samples. Na`ur and Abu Nuseir WWTPs showed high PPCPs removal efficiencies in comparison with AL-Baqa`a, Salt, and Fuhais-Mahis WWTPs. Boqorreya spring showed signs of contamination by Salt WWTP effluents as a result of mixing. Irrigation with effluents showed higher carbamazepine concentrations in soils at the top soil layers (0 to 20 cm) in all farms than its concentrations at the root zone (20 to 40 cm) by using drip irrigation system with various plants. In plants, carbamazepine concentration was only detected in high concentration level in mint leaves. In the same farm, diclofenac concentration was detected only in olives and not in twigs and leaves, indicating a high rate of plant uptake especially during the olive’s growth period. Furthermore, plant fruits, leaves, and stems left on the farm after harvesting are generally consumed by cattle, which means entering the food chain of humans. Full article

Use of antibiotics for the treatment and prevention of bacterial infections in humans, agri- and aquaculture as well as livestock rearing leads to antibiotic pollution of fresh water and these antibiotics have an impact on free-living bacteria. While we know which antibiotics are most common in natural environments such as rivers and streams, there is considerable uncertainty regarding antibiotics’ interactions with one another and the effect of abiotic factors such as temperature. Here, we used an experimental approach to explore the effects of antibiotic identity, concentration, mixing and water temperature on the growth of Pseudomonas fluorescens, a common, ubiquitous bacterium. We exposed P. fluorescens to the four antibiotics most commonly found in surface waters (ciprofloxacin, ofloxacin, sulfamethoxazole and sulfapyridine) and investigated antibiotic interactions for single and mixed treatments at different, field-realistic temperatures. We observed an overall dependence of antibiotic potency on temperature, as temperature increased efficacy of ciprofloxacin and ofloxacin with their EC₅₀ lowered by >75% with a 10 °C temperature increase. Further, we show that mixtures of ciprofloxacin and ofloxacin, despite both belonging to the fluoroquinolone class, exhibit low-temperature-dependent synergistic effects in inhibiting bacterial growth. These findings highlight the context dependency of antibiotic efficacy. They further suggest antibiotic-specific off-target effects that only affect the bacteria once they enter a certain temperature range. This has important implications as freshwater systems already contain multi-drug antibiotic cocktails and are changing temperature due to environmental warming. These factors will interact and affect aquatic food webs, and hence this creates an urgent need to adapt and improve laboratory testing conditions to closer reflect natural environments. Full article

Urban sanitation is a major challenge during the rapid urbanization being experienced by developing countries, as a low sewerage infrastructure capacity and irregular onsite wastewater treatment raise the risk of surface water contamination. The application of specific sewage markers to characterize contaminant sources is therefore essential for managing urban sanitation issues. In this study, we investigated the concentrations of eight sewage markers (acetaminophen, caffeine, carbamazepine, cotinine, sulfamethoxazole, sulfapyridine, atenolol, and acesulfame) in various water sources within urban area of the Galle City, Sri Lanka. The total concentration of the eight markers was in the order of hospital discharge > sewage treatment plant (STP) influent > surface drainage system. Among the eight selected markers, acetaminophen was dominant in hospital discharge (70.2–123.6 µg/L) while caffeine was the largest contributor to STP influent (16.2–68.7 µg/L) and surface drainage (0.95–21.73 µg/L). We then proposed and tested a set of criteria for evaluating the applicability of markers, including removal efficiency, concentration magnitude, excretion rate, and wastewater burden. The labile markers caffeine and acetaminophen were suitable for characterizing domestic gray and black wastewater, respectively. These results imply that the city’s drainage system receives both domestic graywater and human excretion, likely due to insufficient on-site sanitation systems. The conservative marker carbamazepine was useful for tracking hospital residues over long distances; these results imply that hospital wastewater treatment was not working properly, accounting for pharmaceutical residues reaching surface water via a hidden discharges connected to the drainage system. Full article

The presence of antibiotics in crops is mainly caused by their irrigation with reclaimed wastewater and by the use of organic amendments of animal origin. During this work, the fate of sulfonamide antibiotics in tomato crop has been assessed in two commercial greenhouses located in Almería (Spain) irrigated with reclaimed wastewater. Samplings were made annually for two years. Sulfonamides in several parts of the plant (roots, leaves and fruits) as well as reclaimed wastewater, amendments and soils were analyzed by UHPLC-MS/MS. The results showed that sulfonamides accumulated in soils (sulfamethoxazole between 2 and 14 µg kg⁻¹; sulfadiazine, sulfathiazole, sulfapyridine, sulfamerazine and sulfadimethoxine in concentrations below 1 µg kg⁻¹) were in the reclaimed wastewater at concentrations in the ng L⁻¹ range. Their distribution in plants depended on the sulfonamide. The sulfonamides detected in tomato were sulfadiazine, sulfapyridine, sulfamethazole, sulfamethoxazole and sulfadimethoxine. Sulfamethoxazole was the antibiotic with highest concentration in tomato fruit, exceeding 30 µg kg⁻¹. All sulfonamides were below the Acceptable Daily Intake, however, further studies and legislation are needed to assure food safety. Full article

The simultaneous administration of sulfasalazine and folic acid is regular practice in the therapy of inflammatory bowel diseases in order to maintain sufficient folate concentration in patients. Having multiple drugs in the therapy increases the possibility of patients failing adherence, thus unintentionally endangering their health. A fixed-dose combination of sulfasalazine and folic would simplify the classical polytherapeutic approach; however, the physicochemical compatibility investigation of two active pharmaceutical ingredients plays an important role in the development of such a product. In this work, various analytical tools were used to determine the physicochemical compatibility of sulfasalazine and folic acid. For the evaluation of chemical compatibility, infrared spectroscopy in combination with advanced statistical methods, such as the principal component analysis and cluster analysis, were used, whilst a simultaneous thermogravimetry/differential thermal analysis gave us an insight into the physical compatibility of two drugs. Isothermal stress testing, forced degradation and dissolution studies, followed by the analysis with a developed chromatographic method for the monitoring of folic acid, sulfasalazine and two of its related impurities, sulfapyridine and salicylic acid, gave us an insight into its chemical compatibility. The combination of the results obtained from the used techniques implies a satisfactory physicochemical compatibility between sulfasalazine and folic acid, which opens the path to the development of the proposed fixed-dose combination. Full article

Sulfasalazine (SAS), an anti-inflammatory drug with potent cysteine/glutamate antiporter system xc-(SXC) inhibition has recently shown beneficial effects in brain-related diseases. Despite many reports related to central nervous system (CNS) effect of SAS, pharmacokinetics (PK) and metabolite identification studies in the brain for SAS were quite limited. The aim of this study was to investigate the pharmacokinetics and metabolite identification of SAS and their distributions in mouse brain. Using in vivo brain exposure studies (neuro PK), the PK parameters of SAS was calculated for plasma as well as brain following intravenous and oral administration at 10 mg/kg and 50 mg/kg in mouse, respectively. In addition, in vivo metabolite identification (MetID) studies of SAS in plasma and brain were also conducted. The concentration of SAS in brain was much lower than that in plasma and only 1.26% of SAS was detected in mouse brain when compared to the SAS concentration in plasma (brain to plasma ratio (%): 1.26). In the MetID study, sulfapyridine (SP), hydroxy-sulfapyridine (SP-OH), and N-acetyl sulfapyridine (Ac-SP) were identified in plasma, whereas only SP and Ac-SP were identified as significant metabolites in brain. As a conclusion, our results suggest that the metabolites of SAS such as SP and Ac-SP might be responsible for the pharmacological effect in brain, not the SAS itself. Full article

Environmental pollutants vigilance is one of the main problems that the aquaculture industry has to face with the objective to ensure the quality of their products and prevent entrance in the food chain that finally may arrive to the consumer. Contaminants such as hormones, antibiotics or biocides are especially relevant due to their toxicity, pharmacological effect or hormonal activity that can be considered harmful for the final consumer. The contaminants can be detected in the environment where the food is growing, and their concentration can be found (i.e., seawater) in the range of µg·L⁻¹, ng·L⁻¹ or even in lower concentrations. Thus, sensitive and selective methods for their monitoring are required to avoid their arrival in the food chain. Here, the development of a multiplexed amperometric biosensor is described, based on the use of specific antibodies to reach the necessary detectability to measure the targeted contaminants directly in seawater. The multiplexed immunosensor allows the detection of four relevant pollutants, such as el Irgarol 1051, sulfapyridine, chloramphenicol and estradiol, reaching an IC₅₀ of 5.04 ± 0.29, 3.45 ± 0.29, 4.17 ± 0.44 and 5.94 ± 0.28 µg·L⁻¹, directly measured in seawater. Full article

In this work, we report the development of a highly sensitive biosensor for sulfapyridine detection based on an integrated bio micro-electromechanical system (Bio-MEMS) containing four gold working electrodes (WEs), a platinum counter electrode (CE), and a reference electrode (RE). Firstly, the cleaned WEs were modified with 4-aminophenylacetic acid (CMA). Then, (5-[4-(amino)phenylsulfonamide]-5-oxopentanoic acid (SA2BSA) was immobilized onto the transducers surface by carbodiimide chemistry. The analyte was quantified by competitive detection with SA2BSA immobilized on the WE toward a mixture of Ab155 antibody (with fixed concentration) and sulfapyridine. In order to obtain a highly sensitive biosensor, Ab155 was immobilized onto magnetic latex nanoparticles surface to create a 3D architecture (Ab-MLNp). Using electrochemical impedance spectroscopy (EIS), we investigated the influence of the Ab-MLNp on the sensitivity of our approach. The optimized system was analyzed, as competitive assay, with different concentrations of sulfapyridine (40 µM, 4 µM, and 2 nM) and with phosphate buffer solution. From data fitting calculations and graphs, it was observed that the EIS showed more linearity when Ab-MLNp was used. This result indicates that the magnetic latex nanoparticles increased the sensitivity of the biosensor. Full article