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Development of Novel Magneto-Biosensor for Sulfapyridine Detection

Institut des Sciences Analytiques, University Claude Bernard Lyon-1, UMR 5280, 5 rue de la Doua, F-69100 Villeurbanne, France
University Claude Bernard Lyon-1, CNRS, LAGEPP UMR-5007, 43 Boulevard du 11 Novembre 1918, F-69100 Villeurbanne, France
Faculty of Pharmacy and Alternatives Medicins, The Islamia University of Bahawalpur, Bahawalpur 63100, Pakistan
Department of Chemistry, State University of Ponta Grossa, Av. Gen. Carlos Cavalcanti, 4748, CEP 84030-900 Ponta Grossa, Paraná, Brazil
Nanobiotechnology for Diagnostics (Nb4D), Department of Chemical and Biomolecular Nanotechnology, Institute for Advanced Chemistry of Catalonia (IQAC) of the Spanish Council for Scientific Research (CSIC), Jordi Girona 18-26, 08034 Barcelona, Spain
CIBER de Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN), Jordi Girona 18-26, 08034 Barcelona, Spain
Institute of Microelectronics of Barcelona (IMB−CSIC), Universitat Autònoma de Barcelona (UAB), Bellaterra, 08193 Barcelona, Spain
Authors to whom correspondence should be addressed.
Biosensors 2020, 10(4), 43;
Received: 25 March 2020 / Revised: 15 April 2020 / Accepted: 17 April 2020 / Published: 21 April 2020
(This article belongs to the Special Issue Portable Technologies toward ASSURED Biosensing and Diagnostics)
In this work, we report the development of a highly sensitive biosensor for sulfapyridine detection based on an integrated bio micro-electromechanical system (Bio-MEMS) containing four gold working electrodes (WEs), a platinum counter electrode (CE), and a reference electrode (RE). Firstly, the cleaned WEs were modified with 4-aminophenylacetic acid (CMA). Then, (5-[4-(amino)phenylsulfonamide]-5-oxopentanoic acid (SA2BSA) was immobilized onto the transducers surface by carbodiimide chemistry. The analyte was quantified by competitive detection with SA2BSA immobilized on the WE toward a mixture of Ab155 antibody (with fixed concentration) and sulfapyridine. In order to obtain a highly sensitive biosensor, Ab155 was immobilized onto magnetic latex nanoparticles surface to create a 3D architecture (Ab-MLNp). Using electrochemical impedance spectroscopy (EIS), we investigated the influence of the Ab-MLNp on the sensitivity of our approach. The optimized system was analyzed, as competitive assay, with different concentrations of sulfapyridine (40 µM, 4 µM, and 2 nM) and with phosphate buffer solution. From data fitting calculations and graphs, it was observed that the EIS showed more linearity when Ab-MLNp was used. This result indicates that the magnetic latex nanoparticles increased the sensitivity of the biosensor. View Full-Text
Keywords: biosensor; sulfapyridine; SA2BSA; BioMEMS; magnetic nanoparticles; competitive assay biosensor; sulfapyridine; SA2BSA; BioMEMS; magnetic nanoparticles; competitive assay
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MDPI and ACS Style

Jamshaid, T.; Tenório-Neto, E.T.; Baraket, A.; Lebaz, N.; Elaissari, A.; Sanchís, A.; Salvador, J.-P.; Marco, M.-P.; Bausells, J.; Errachid, A.; Zine, N. Development of Novel Magneto-Biosensor for Sulfapyridine Detection. Biosensors 2020, 10, 43.

AMA Style

Jamshaid T, Tenório-Neto ET, Baraket A, Lebaz N, Elaissari A, Sanchís A, Salvador J-P, Marco M-P, Bausells J, Errachid A, Zine N. Development of Novel Magneto-Biosensor for Sulfapyridine Detection. Biosensors. 2020; 10(4):43.

Chicago/Turabian Style

Jamshaid, Talha, Ernandes Taveira Tenório-Neto, Abdoullatif Baraket, Noureddine Lebaz, Abdelhamid Elaissari, Ana Sanchís, J.-Pablo Salvador, M.-Pilar Marco, Joan Bausells, Abdelhamid Errachid, and Nadia Zine. 2020. "Development of Novel Magneto-Biosensor for Sulfapyridine Detection" Biosensors 10, no. 4: 43.

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