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Open AccessArticle

Development of Novel Magneto-Biosensor for Sulfapyridine Detection

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Institut des Sciences Analytiques, University Claude Bernard Lyon-1, UMR 5280, 5 rue de la Doua, F-69100 Villeurbanne, France
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University Claude Bernard Lyon-1, CNRS, LAGEPP UMR-5007, 43 Boulevard du 11 Novembre 1918, F-69100 Villeurbanne, France
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Faculty of Pharmacy and Alternatives Medicins, The Islamia University of Bahawalpur, Bahawalpur 63100, Pakistan
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Department of Chemistry, State University of Ponta Grossa, Av. Gen. Carlos Cavalcanti, 4748, CEP 84030-900 Ponta Grossa, Paraná, Brazil
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Nanobiotechnology for Diagnostics (Nb4D), Department of Chemical and Biomolecular Nanotechnology, Institute for Advanced Chemistry of Catalonia (IQAC) of the Spanish Council for Scientific Research (CSIC), Jordi Girona 18-26, 08034 Barcelona, Spain
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CIBER de Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN), Jordi Girona 18-26, 08034 Barcelona, Spain
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Institute of Microelectronics of Barcelona (IMB−CSIC), Universitat Autònoma de Barcelona (UAB), Bellaterra, 08193 Barcelona, Spain
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Authors to whom correspondence should be addressed.
Biosensors 2020, 10(4), 43; https://doi.org/10.3390/bios10040043
Received: 25 March 2020 / Revised: 15 April 2020 / Accepted: 17 April 2020 / Published: 21 April 2020
(This article belongs to the Special Issue Portable Technologies toward ASSURED Biosensing and Diagnostics)
In this work, we report the development of a highly sensitive biosensor for sulfapyridine detection based on an integrated bio micro-electromechanical system (Bio-MEMS) containing four gold working electrodes (WEs), a platinum counter electrode (CE), and a reference electrode (RE). Firstly, the cleaned WEs were modified with 4-aminophenylacetic acid (CMA). Then, (5-[4-(amino)phenylsulfonamide]-5-oxopentanoic acid (SA2BSA) was immobilized onto the transducers surface by carbodiimide chemistry. The analyte was quantified by competitive detection with SA2BSA immobilized on the WE toward a mixture of Ab155 antibody (with fixed concentration) and sulfapyridine. In order to obtain a highly sensitive biosensor, Ab155 was immobilized onto magnetic latex nanoparticles surface to create a 3D architecture (Ab-MLNp). Using electrochemical impedance spectroscopy (EIS), we investigated the influence of the Ab-MLNp on the sensitivity of our approach. The optimized system was analyzed, as competitive assay, with different concentrations of sulfapyridine (40 µM, 4 µM, and 2 nM) and with phosphate buffer solution. From data fitting calculations and graphs, it was observed that the EIS showed more linearity when Ab-MLNp was used. This result indicates that the magnetic latex nanoparticles increased the sensitivity of the biosensor. View Full-Text
Keywords: biosensor; sulfapyridine; SA2BSA; BioMEMS; magnetic nanoparticles; competitive assay biosensor; sulfapyridine; SA2BSA; BioMEMS; magnetic nanoparticles; competitive assay
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Jamshaid, T.; Tenório-Neto, E.T.; Baraket, A.; Lebaz, N.; Elaissari, A.; Sanchís, A.; Salvador, J.-P.; Marco, M.-P.; Bausells, J.; Errachid, A.; Zine, N. Development of Novel Magneto-Biosensor for Sulfapyridine Detection. Biosensors 2020, 10, 43.

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