Search Results (978)

Background/Objectives: In recent years, lipids have emerged as critical regulators of different disease processes, being involved in cancer pathogenesis, progression, and outcome. Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry Imaging (MALDI-MSI) has significantly expanded the technology’s reach, enabling spatially resolved profiling of lipids directly from tissue, including formalin-fixed paraffin-embedded (FFPE) specimens. In this context, MALDI matrix selection is crucial for lipid extraction and ionization, influencing key aspects such as molecular coverage and sensitivity, especially in such specimens with already depleted lipid content. Thus, in this work, we aim to explore the feasibility of mapping lipid species in FFPE clinical samples with MALDI-MSI using 6-aza-2-thiothymine (ATT) as a matrix of choice. Methods: To do so, ATT performances were first compared to those two other matrices commonly used for lipidomic analyses, 2′,5′-dihydroxybenzoic acid (DHB) and Norharmane (NOR), on lipid standards. Results: As a proof-of-concept, we then assessed ATT’s performance for the MALDI-MSI analysis of lipids in FFPE brain sections, both in positive and negative ion modes, comparing results with those obtained from other commonly used dual-polarity matrices. In this context, ATT enabled the putative annotation of 98 lipids while maintaining a well-balanced detection of glycerophospholipids (60.2%) and sphingolipids (32.7%) in positive ion mode. It outperformed both DHB and NOR in the identification of glycolipids (3%) and fatty acids (4%). Additionally, ATT exceeded DHB in terms of total lipid count (62 vs. 21) and class diversity and demonstrated performance comparable to NOR in negative ion mode. Moreover, ATT was applied to a FFPE glioblastoma tissue microarray (TMA) evaluating the ability of this matrix to reveal biologically relevant lipid features capable of distinguishing normal brain tissue from glioblastoma regions. Conclusions: Altogether, the results presented in this work suggest that ATT is a suitable matrix for pathology imaging applications, even at higher lateral resolutions of 20 μm, not only for proteomic but also for lipidomic analysis. This could enable the use of the same matrix type for the analysis of both lipids and peptides on the same tissue section, offering a unique strategic advantage for multi-omics studies, while also supporting acquisition in both positive and negative ionization modes. Full article

Phage therapy, long overshadowed by antibiotics in Western medicine, has a well-established history in some Eastern European countries and is now being revitalized as a promising strategy against antimicrobial resistance (AMR). This resurgence of phage therapy is driven by the urgent need for innovative countermeasures to AMR, which will cause an estimated 10 million deaths annually by 2050. However, the emergence of phage-resistant variants presents challenges similar to AMR, thus necessitating a deeper understanding of phage resistance mechanisms and control strategies. The highest priority must be to prevent the emergence of phage resistance. Although phage cocktails targeting multiple receptors have demonstrated a certain level of phage resistance suppression, they cannot completely suppress resistance in clinical settings. This highlights the need for strategies beyond simple resistance suppression. Notably, recent studies examining fitness trade-offs associated with phage resistance have opened new avenues in phage therapy that offer the potential of restoring antibiotic susceptibility and attenuating pathogen virulence despite phage resistance. Thus, controlling phage resistance may rely on both its suppression and strategic redirection. This review summarizes key concepts in the control of phage resistance and explores evolutionary engineering as a means of optimizing phage therapy, with a particular focus on Pseudomonas infections. Harnessing evolutionary dynamics by intentionally breaking the spontaneous evolutionary trajectories of target bacterial pathogens could potentially reshape bacterial adaptation by acquisition of phage resistance, unlocking potential in the application of phage therapy. Full article

This theoretical essay aims to analyze some of the socio-economic innovations introduced by Platform Capitalism Specifically, it focuses on two main aspects: first, the digital platform as a radical organizational innovation. Digital platforms represent a structural novelty in the market economy, signaling a new organization of production and labor. Second, the essay examines the role of platforms in directly generating value through the concept of “network value”. To this end, it explores the function of “business intelligence” as a strategic and competitive tool. Finally, the paper discusses the key issues associated with platform capitalism, which could threaten its social sustainability and contribute to economic and financial instability. These issues include the increasing commodification of everyday activities, the devaluation of paid labor in favor of free production driven by platform users (the so-called prosumers), and the emergence of proprietary and financial monopolies. Hence, digital platforms do not inherently ensure comprehensive social and environmental sustainability unless supported by targeted economic policy interventions. Conclusively, it is emphasized that defining robust social welfare frameworks—which account for emerging value creation processes—is imperative. Simultaneously, policymakers must incentivize the proliferation of cooperative platforms capable of fostering experimental circular economy models aligned with ecological sustainability. Full article

Entropy is a foundational concept across scientific domains, playing a role in understanding disorder, randomness, and uncertainty within systems. This study applies Shannon’s entropy in information theory to evaluate and manage complexity in industrial supply chain management. The purpose of the study is to propose a quantitative modeling method, employing Shannon’s entropy model as a proxy to assess the complexity in SCs. The underlying assumption is that information entropy serves as a proxy for the complexity of the SC. The research method is quantitative modeling, which is applied to four focal companies from the agrifood and metalworking industries in Southern Brazil. The results showed that companies prioritizing cost and quality exhibit lower complexity compared to those emphasizing flexibility and dependability. Additionally, information flows related to specially engineered products and deliveries show significant differences in average entropies, indicating that organizational complexities vary according to competitive priorities. The implications of this suggest that a focus on cost and quality in SCM may lead to lower complexity, in opposition to a focus on flexibility and dependability, influencing strategic decision making in industrial contexts. This research introduces the novel application of information entropy to assess and control complexity within industrial SCs. Future studies can explore and validate these insights, contributing to the evolving field of supply chain management. Full article

Early evacuation during bushfires remains the safest strategy; however, in many realistic scenarios, timely evacuation is challenging, making safe sheltering a last-resort option to reduce risk compared to late evacuation attempts. However, most Australian homes in bushfire-prone areas are neither designed nor retrofitted to provide adequate protection against extreme bushfires, raising safety concerns. This study addresses this gap by investigating the concept of retrofitting a part of the residential buildings as attached safe rooms for sheltering and protection of valuables, providing a potential last-resort solution for bushfire-prone communities. Numerical simulations were conducted using the Fire Dynamics Simulator to assess heat transfer and internal temperature conditions in a representative residential building under bushfire exposure conditions. The study investigated the impact of the placement of the safe room relative to the fire front direction, failure of vulnerable building components, and the effectiveness of steel shutters in response to internal temperatures. The results showed that the strategic placement of safe rooms inside the building, along with adequate protective measures for windows, can substantially reduce internal temperatures. The findings emphasised the importance of maintaining the integrity of openings and the external building envelope, demonstrating the potential of retrofitted attached safe rooms as a last-resort solution for existing residential buildings in bushfire-prone areas where the entire building was not constructed to withstand bushfire conditions. Full article

In recent years, the concept of the myofascial network has transformed biomechanical understanding by emphasizing the body as an integrated, multidirectional system. This study advances that paradigm by applying graph theory to model the osteo-myofascial system as an anatomical network, enabling the identification of topologically central nodes involved in force transmission, stability, and coordination. Using the aNETomy model and the BIOMECH 3.4 database, we constructed an undirected network of 2208 anatomical nodes and 7377 biomechanical relationships. Centrality analysis (degree, betweenness, and closeness) revealed that structures such as the sacrum and thoracolumbar fascia exhibit high connectivity and strategic importance within the network. These findings, while derived from a theoretical modeling approach, suggest that such key nodes may inform targeted treatment strategies, particularly in complex or compensatory musculoskeletal conditions. The proposed concept of a polyconnective skeleton (PCS) synthesizes the most influential anatomical hubs into a functional core of the system. This framework may support future clinical and technological applications, including integration with imaging modalities, real-time monitoring, and predictive modeling for personalized and preventive medicine. Full article

The widespread adoption of cloud computing has amplified the demand for electric power. It is strategically important to address the limitations of reliable sources and sustainability of power. Research and investment in data centres and power infrastructure are therefore critically important for our digital economy. A novel heuristic for the minimisation of energy consumption in cloud computing is presented. It draws similarities to the concept of “network slices”, in which an orchestrator enables multiplexing to reduce the network “churn” often associated with significant losses of energy consumption. The novel network slicing–ring fencing ratio is a heuristic calculated through an iterative procedure for the reduction in cloud energy consumption. Simulation results show how the non-convex equation optimises power by reducing energy from 10,680 kJ to 912 kJ, which is a 91.46% efficiency gain. In comparison, the Heuristic AUGMENT Non-Convex algorithm (HA-NC, by Hossain and Ansari) reported a 312.74% increase in energy consumption from 2464 kJ to 10,168 kJ, while the Priority Selection Offloading algorithm (PSO, by Anajemba et al.) also reported a 150% increase in energy consumption, from 10,738 kJ to 26,845 kJ. The proposed network slicing–ring fencing ratio is seen to successfully balance energy consumption and computing performance. We therefore think the novel approach could be of interest to network architects and cloud operators. Full article

This paper analyzes the concept of packaging redesign, with the primary objective of improving material utilization. It further examines the potential environmental and economic benefits that may result from effective packaging redesign for both industry and society. The research is based on a specific case study comparing two alternative bottle designs with identical capacity, focusing on shape, material usage, and space efficiency. A detailed numerical comparison highlights the advantages and disadvantages of each option. The analysis demonstrates that an optimized bottle design can lead to substantial material savings and waste reduction. For example, an 8% reduction in bottle weight could eliminate approximately 1.6 million tons of material annually, potentially translating into economic savings exceeding 3 billion U.S. dollars per year. The study underscores how strategic packaging redesign can yield significant benefits in terms of material efficiency and cost savings for companies. It also contributes to the field of Life Cycle Analysis by linking packaging design innovation to key environmental and economic outcomes, while ensuring that packaging continues to protect products and meet the needs of the end consumer. Full article

West Africa faces persistent food and nutrition insecurity despite agricultural efforts, exacerbated by population growth, climate change, and socio-economic vulnerabilities. This study argues that integrating food processing principles with nutrition-sensitive agriculture (NSA) and nutrition-smart agriculture (NSmartAg) offers a transformative solution for human health. Therefore, we delineate these interconnected concepts and highlight their synergistic potential for a nutrition-focused food system. Likewise, critical analysis of key regional challenges, including infrastructural weaknesses, policy gaps, and gender inequities, was made prior to identifying significant opportunities for leveraging food processing as a strategic entry point to accelerate the implementation of NSA and NSmartAg. Based on these insights, six strategic pathways are proposed to achieve this objective: (i) integrating food processing into policies; (ii) investing in interdisciplinary R&D that puts nutrition and health benefits at the forefront of desired outcomes along with others; (iii) strengthening farmer and food processor capacities; (iv) improving agri-food infrastructure; (v) fostering multi-sectoral collaboration; and (vi) prioritizing youth engagement and market development. By adopting these integrated strategies, West African countries can build more resilient, equitable, and nutrition-centered food systems, ultimately improving public health outcomes and fostering sustainable regional development. Full article

How to promote sustainable development and deal with the actual development demands in economic transformation through land-use planning is crucial for local governments. The urban sustainable development mainly relies on creativity and talents in the digital era, and talents are increasingly attracted by local people-oriented land use. However, the current planning ideology remains at meeting corporate and people’s basic needs rather than specific needs of talents, especially the increasingly emerging digital creatives. To promote the talent agglomeration and sustainable development through land planning, this paper uses micro-celebrities on Bilibili, an influential creative content creation platform among young people in China, as an example to study the geographical distribution of digital creative talents and its relationship with urban amenities by constructing an index system of urban amenities, comprising natural, leisure, infrastructure, and social and institutional amenities. The concept of borrowed amenities is introduced to examine the effects of amenities of surrounding cities. This study demonstrates that micro-celebrities show a stronger preference for amenities compared with other skilled talents. Meanwhile, social and institutional amenities are most crucial. Furthermore, urban leisure represented by green spaces and consumption spaces is also attractive. At the regional scale, with prefecture-level cities as units, the local talents agglomeration is also influenced by the borrowed amenities in the context of regional integration. It indicates that the local land use should consider the characteristics of the surrounding cities. This study provides strategic inspiration that a happy and sustainable city should first be people-oriented and provide sufficient space for consumption, entertainment, and interaction. Full article

For high-growth firms, designing and implementing strategies to ensure the long-term sustainability of business models is a key priority. Although these strategies are carefully planned to achieve specific outcomes, these firms also encounter contextual factors inherent to entrepreneurship, as well as the potential negative consequences of operating as small- and medium-sized enterprises (SMEs). Consequently, they adapt emergent outcomes to secure positive scaling-up processes. A comprehensive analysis of 69 studies from 1978 to 2023 revealed that 34.8% used sales as the main indicator of high-growth outcomes, 18.8% considered employment to be the most important outcome, and 37.7% incorporated both. The assessment period for these studies spanned three to seven consecutive years. A subsequent review of the existing literature yielded 56 potential new outcomes, emphasising the existence of a diverse array of concepts and metrics with which to assess high-growth performance. The study confirmed sales and positive profits arising during the planning process as strategic outcomes. However, it was also demonstrated that geographical expansion and innovation become emergent outcomes in critical situations. The research also identified that external factors, including an adverse public environment, business context difficulties, and a favourable business environment, may influence the effect of the firm’s high growth. Full article

The subject of this research is the original project concept of the life-centered city, which focuses on the planning and design of sustainable solutions for urban landscape transformation. This concept prioritizes the well-being and needs of all life on Earth, including not only humans but also animals and their natural habitats. The aim of this article is to propose ways to implement the life-centered city concept into the strategic development policies of cities and identify sustainable urban landscape solutions that foster the creation of interspecies spaces. The research employs a comparative analysis of selected European cities, neighborhoods, and urban microspaces that are progressively adapting to climate change, addressing the needs of various users, and prioritizing the development of interspecies spaces. A detailed study focuses on the Polish city of Gliwice, which serves as a pilot example of applying the life-centered city model to local landscapes. Our findings suggest that the life-centered city concept, when effectively integrated into city development strategies and implemented within the urban fabric, can act as a proactive tool for transforming urban landscapes to better accommodate both people and nature. It supports the creation of a sustainable built environment that is inclusive, resilient, and adaptable to change. Full article

Energy-intensive industries (EIIs) face mounting pressure to reduce greenhouse gas emissions while maintaining international competitiveness—a balance that is central to achieving the EU’s 2030 and 2050 climate objectives. In this context, energy management (EM) emerges as a strategic instrument to decouple industrial growth from fossil energy consumption. This study proposes a redefinition of EM to support carbon-neutral supply chains within the European Union’s EIIs, addressing critical limitations of conventional EM frameworks under increasingly stringent carbon regulations. Using a modified systematic literature review based on PRISMA methodology, complemented by expert insights from EU Member States, this research identifies structural gaps in current EM practices and highlights opportunities for integrating sustainable innovations across the whole industrial value chain. The proposed EM concept is validated through an analysis of 24 EM definitions, over 170 scientific publications, and over 80 EU legal and strategic documents. The framework incorporates advanced digital technologies—including artificial intelligence (AI), the Internet of Things (IoT), and big data analytics—to enable real-time optimisation, predictive control, and greater system adaptability. Going beyond traditional energy efficiency, the redefined EM encompasses the entire energy lifecycle, including use, transformation, storage, and generation. It also incorporates social dimensions, such as corporate social responsibility (CSR) and stakeholder engagement, to cultivate a culture of environmental stewardship within EIIs. This holistic approach provides a strategic management tool for optimising energy use, reducing emissions, and strengthening resilience to regulatory, environmental, and market pressures, thereby promoting more sustainable, inclusive, and transparent supply chain operations. Full article

This paper explores the strategic implications of technological adoption within shipping logistics service supply chains, with a particular focus on blockchain technology (BCT). When integrating new technologies, supply chain stakeholders evaluate associated risks alongside complexity, profitability, and operational challenges, which influence their strategic behaviors. Anchored in the concept of technology trust, this study examines how different risk preferences affect BCT adoption decisions and freight rate strategies. A game-theoretic model is constructed using a mean-variance utility framework to analyze interactions between shipping companies and freight forwarders under three adoption scenarios: no adoption (NN), partial adoption (BN), and full adoption (BB). The results indicate that risk-seeking agents are more likely to adopt BCT early but face greater freight rate volatility in the initial stages. As the technology matures, strategic variability declines and the influence of adaptability on pricing becomes less pronounced. In contrast, risk-neutral and risk-averse participants tend to adopt more conservatively, resulting in slower but more stable pricing dynamics. These findings offer new insights into how technology trust and risk attitudes shape strategic decisions in digitally transforming supply chains. The study also provides practical implications for differentiated pricing strategies, BCT adoption incentives, and collaborative policy design among logistics stakeholders. Full article

The primary objective of this paper is to quantify a realistic pathway for Canada to reach net-zero emissions by 2050. This study analyzed greenhouse gas (GHG) emissions from the 10 provinces and 3 territories of Canada based on the emissions from their economic sectors. A time series analysis was performed to understand the trajectory of the emissions profile from 1990 to 2023. Using the 2023 emissions as the baseline, a linear reduction, based on the GHG proportions from each jurisdiction, was performed and projected to 2050 (except for Prince Edward Island (PEI), where net zero was targeted for 2040). Moreover, a machine learning technique (k-means unsupervised algorithm) was used to group all the jurisdictions into homogeneous regions for national strategic climate policy initiatives. The within-cluster sum of squares identified the following clusters: Cluster 1: Manitoba (MB), New Brunswick, Nova Scotia, and Newfoundland and Labrador; Cluster 2: Alberta (AB); Cluster 3: Quebec (QC) and Saskatchewan; Cluster 4: Ontario (ON); and Cluster 5: PEI, Northwest Territories, Nunavut, and Northwest Territories. Considering the maximum GHG reductions needed per cluster (Clusters 1–5), the results show that 0.309 Mt CO₂ eq/year, 5.447 Mt CO₂ eq/year, 1.293 Mt CO₂ eq/year, 2.217 Mt CO₂ eq/year, and 0.04 Mt CO₂ eq/year must be targeted from MB (transportation), AB (stationary combustion), QC (transportation), ON (stationary combustion) and PEI (transportation), respectively. The concept of climate stabilization wedges, which provides a practical framework for addressing the monumental challenge of mitigating climate change, was introduced to each derived region to cut GHG emissions in Canada through tangible, measurable actions that is specific to each sector/cluster. The clustering-based method breaks climate mitigation problems down into manageable pieces by grouping the jurisdictions into efficient regions that can be managed effectively by fostering collaboration across jurisdictions and economic sectors. Actionable and strategic recommendations were made within each province to reach the goal of net-zero. The implications of this study for policy and climate action include the fact that actionable strategies and tailored policies are applied to each cluster’s emission profile and economic sector, ensuring equitable and effective climate mitigation strategies in Canada. Full article