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29 pages, 6079 KiB  
Article
A Highly Robust Terrain-Aided Navigation Framework Based on an Improved Marine Predators Algorithm and Depth-First Search
by Tian Lan, Ding Li, Qixin Lou, Chao Liu, Huiping Li, Yi Zhang and Xudong Yu
Drones 2025, 9(8), 543; https://doi.org/10.3390/drones9080543 (registering DOI) - 31 Jul 2025
Abstract
Autonomous underwater vehicles (AUVs) have obtained extensive application in the exploitation of marine resources. Terrain-aided navigation (TAN), as an accurate and reliable autonomous navigation method, is commonly used for AUV navigation. However, its accuracy degrades significantly in self-similar terrain features or measurement uncertainties. [...] Read more.
Autonomous underwater vehicles (AUVs) have obtained extensive application in the exploitation of marine resources. Terrain-aided navigation (TAN), as an accurate and reliable autonomous navigation method, is commonly used for AUV navigation. However, its accuracy degrades significantly in self-similar terrain features or measurement uncertainties. To overcome these challenges, we propose a novel terrain-aided navigation framework integrating an Improved Marine Predators Algorithm with Depth-First Search optimization (DFS-IMPA-TAN). This framework maintains positioning precision in partially self-similar terrains through two synergistic mechanisms: (1) IMPA-driven optimization based on the hunger-inspired adaptive exploitation to determine optimal trajectory transformations, cascaded with Kalman filtering for navigation state correction; (2) a Robust Tree (RT) hypothesis manager that maintains potential trajectory candidates in graph-structured memory, employing Depth-First Search for ambiguity resolution in feature matching. Experimental validation through simulations and in-vehicle testing demonstrates the framework’s distinctive advantages: (1) consistent terrain association in partially self-similar topographies; (2) inherent error resilience against ambiguous feature measurements; and (3) long-term navigation stability. In all experimental groups, the root mean squared error of the framework remained around 60 m. Under adverse conditions, its navigation accuracy improved by over 30% compared to other traditional batch processing TAN methods. Comparative analysis confirms superior performance over conventional methods under challenging conditions, establishing DFS-IMPA-TAN as a robust navigation solution for AUVs in complex underwater environments. Full article
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14 pages, 2200 KiB  
Article
Tree Species as Metabolic Indicators: A Comparative Simulation in Amman, Jordan
by Anas Tuffaha and Ágnes Sallay
Land 2025, 14(8), 1566; https://doi.org/10.3390/land14081566 - 31 Jul 2025
Abstract
Urban metabolism frameworks offer insight into flows of energy, materials, and services in cities, yet tree species selection is seldom treated as a metabolic indicator. In Amman, Jordan, we integrate spatial metabolic metrics to critique monocultural greening policies and demonstrate how species choices [...] Read more.
Urban metabolism frameworks offer insight into flows of energy, materials, and services in cities, yet tree species selection is seldom treated as a metabolic indicator. In Amman, Jordan, we integrate spatial metabolic metrics to critique monocultural greening policies and demonstrate how species choices forecast long-term urban metabolic performance. Using ENVI-met 5.61 simulations, we compare Melia azedarach, Olea europaea, and Ceratonia siliqua, mainly assessing urban flow related elements like air temperature reduction, CO2 sequestration, and evapotranspiration alongside rooting depth, isoprene emissions, and biodiversity support. Melia delivers rapid cooling but shows other negatives like a low biodiversity value; Olea offers average cooling and sequestration but has allergenic pollen issues in people as a flow; Ceratonia provides scalable cooling, increased carbon uptake, and has a high ecological value. We propose a metabolic reframing of green infrastructure planning to choose urban species, guided by system feedback rather than aesthetics, to ensure long-term resilience in arid urban climates. Full article
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22 pages, 2718 KiB  
Article
Enhancing the Analysis of Rheological Behavior in Clinker-Aided Cementitious Systems Through Large Language Model-Based Synthetic Data Generation
by Murat Eser, Yahya Kaya, Ali Mardani, Metin Bilgin and Mehmet Bozdemir
Materials 2025, 18(15), 3579; https://doi.org/10.3390/ma18153579 - 30 Jul 2025
Abstract
This study investigates the parameters influencing the compatibility between cement and polycarboxylate ether (PCE) admixtures in cements produced with various types and dosages of grinding aids (GAs). A total of 29 cement types (including a control) were prepared using seven different GAs at [...] Read more.
This study investigates the parameters influencing the compatibility between cement and polycarboxylate ether (PCE) admixtures in cements produced with various types and dosages of grinding aids (GAs). A total of 29 cement types (including a control) were prepared using seven different GAs at four dosage levels, and 87 paste mixtures were produced with three PCE dosages. Rheological behavior was evaluated via the Herschel–Bulkley model, focusing on dynamic yield stress (DYS) and viscosity. The data were modeled using CNN, Random Forest (RF), and Neural Classification and Regression Tree (NCART), and each model was enhanced with synthetic data generated by Large Language Models (LLMs), resulting in CNN-LLM, RF-LLM, and NCART-LLM variants. All six variants were evaluated using R-squared, Mean Absolute Error (MAE), Root Mean Square Error (RMSE), and Logcosh. This study is among the first to use LLMs for synthetic data augmentation. It augmented the experimental dataset synthetically and analyzed the effects on the study results. Among the baseline methods, NCART achieved the best performance for both viscosity (MAE = 1.04, RMSE = 1.33, R2 = 0.84, Logcosh = 0.57) and DYS (MAE = 8.73, RMSE = 11.50, R2 = 0.77, Logcosh = 8.09). Among baseline models, NCART performed best, while LLM augmentation significantly improved all models’ predictive accuracy. It was also observed that cements produced with GA exhibited higher DYS and viscosity than the control, likely due to finer particle size distribution. Overall, the study highlights the potential of LLM-based synthetic augmentation in modeling cement admixture compatibility. Full article
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14 pages, 1742 KiB  
Article
Characterization of Biological Components of Leaves and Flowers in Moringa peregrina and Their Effect on Proliferation of Staurogyne repens in Tissue Culture Conditions
by Hamideh Khajeh, Bahman Fazeli-Nasab, Ali Salehi Sardoei, Zeinab Fotoohiyan, Mehrnaz Hatami, Alireza Mirzaei, Mansour Ghorbanpour and Filippo Maggi
Plants 2025, 14(15), 2340; https://doi.org/10.3390/plants14152340 - 29 Jul 2025
Viewed by 155
Abstract
Moringa peregrina (Forssk.) Fiori is a tropical tree in southern Iran known as the most important natural coagulant in the world. Today, plant tissue culture is a new method that has a very high potential to produce valuable medicinal compounds on a commercial [...] Read more.
Moringa peregrina (Forssk.) Fiori is a tropical tree in southern Iran known as the most important natural coagulant in the world. Today, plant tissue culture is a new method that has a very high potential to produce valuable medicinal compounds on a commercial level. Advances in in vitro cultivation methods have increased the usefulness of plants as renewable resources. In this study, in addition to the phytochemical analysis of the extract of M. peregrina using HPLC, the interaction effect of different concentrations of aqueous extract of M. peregrina (0, 1, 1.5, and 3 mg/L) in two types of MS and ½ MS basal culture media over three weeks on the in vitro growth of Staurogyne repens (Nees) Kuntze was studied. The amounts of quercetin, gallic acid, caffeic acid, and myricetin in the aqueous extract of M. peregrina were 64.9, 374.8, 42, and 4.6 mg/g, respectively. The results showed that using M. peregrina leaf aqueous extract had a positive effect on the length of the branches, the percentage of green leaves, rooting, and the fresh and dry weight of S. repens samples. The highest increase in growth indices was observed in the MS culture medium supplemented with 3 mg/L of M. peregrina leaf aqueous extract after three weeks of cultivation. Of course, this effect was significantly greater in the MS medium and at higher concentrations compared to the ½ MS medium. Three weeks after cultivation at a concentration of 3 mg/L of the extract, the length of the S. repens branches was 5.3 and 1.8 cm in the two basic MS and ½ MS culture media, and the percentage of green leaves was 14 and 4 percent, respectively. Also, rooting was measured at 9.6 and 3.6 percent, fresh weight at 6 and 1.4 g, and dry weight at 1.1 and 0.03 g, respectively. Therefore, adding M. peregrina leaf aqueous extract as a stimulant significantly increased the in vitro growth of S. repens. Full article
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13 pages, 1606 KiB  
Article
The Correlation of Microscopic Particle Components and Prediction of the Compressive Strength of Fly-Ash-Based Bubble Lightweight Soil
by Yaqiang Shi, Hao Li, Hongzhao Li, Zhiming Yuan, Wenjun Zhang, Like Niu and Xu Zhang
Buildings 2025, 15(15), 2674; https://doi.org/10.3390/buildings15152674 - 29 Jul 2025
Viewed by 140
Abstract
Fly-ash-based bubble lightweight soil is widely used due to its environmental friendliness, load reduction, ease of construction, and low costs. In this study, 41 sets of 28 d compressive strength data on lightweight soils with different water–cement ratios, blowing agent dosages, and fly [...] Read more.
Fly-ash-based bubble lightweight soil is widely used due to its environmental friendliness, load reduction, ease of construction, and low costs. In this study, 41 sets of 28 d compressive strength data on lightweight soils with different water–cement ratios, blowing agent dosages, and fly ash dosages were collected through a literature search and indoor tests. Using the compressive strength index and SEM tests, the correlation between the mix ratio design and the microscopic particle components was investigated. The findings were as follows: carbonation reactions occurred in lightweight soil during the maintenance process, and the particles were spherical; increasing the dosage of blowing agent increased the soil’s porosity and pore diameter, leading to the formation of through-holes and reducing the compressive strength and mobility; increasing the fly ash dosage and water–cement ratio increased the soil’s mobility but reduced its compressive strength; and the strength decreased significantly when the fly ash dosage was more than 16% (e.g., the strength at a 20% dosage was 17.8% lower than that at a 15% dosage). Feature importance analysis showed that the water–cement ratio (57.7%), fly ash dosage (30.9%), and blowing agent dosage (11.1%) had a significant effect on strength. ExtraTrees, LightGBM, and Bayesian-optimized Random Forest models were used for 28d strength prediction with coefficients of determination (R2) of 0.695, 0.731, and 0.794, respectively. The Bayesian-optimized Random Forest model performed optimally in terms of the mean square error (MSE), root mean square error (RMSE), and mean absolute error (MAE), and the prediction performance was best. The accuracy of the model is expected to be further improved with expansions in the database. A 28 d compressive strength prediction platform for fly-ash-based bubble lightweight soil was ultimately developed, providing a convenient tool for researchers and engineers to predict material properties and mix ratios. Full article
(This article belongs to the Section Building Materials, and Repair & Renovation)
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17 pages, 2446 KiB  
Article
Different Phosphorus Preferences Among Arbuscular and Ectomycorrhizal Trees with Different Acquisition Strategies in a Subtropical Forest
by Yaping Zhu, Jianhua Lv, Pifeng Lei, Miao Chen and Jinjuan Xie
Forests 2025, 16(8), 1241; https://doi.org/10.3390/f16081241 - 28 Jul 2025
Viewed by 113
Abstract
Phosphorus (P) availability is a major constraint on plant growth in many forest ecosystems, yet the strategies by which different tree species acquire and utilize various forms of soil phosphorus remain poorly understood. This study investigated how coexisting tree species with contrasting mycorrhizal [...] Read more.
Phosphorus (P) availability is a major constraint on plant growth in many forest ecosystems, yet the strategies by which different tree species acquire and utilize various forms of soil phosphorus remain poorly understood. This study investigated how coexisting tree species with contrasting mycorrhizal types, specifically arbuscular mycorrhizal (AM) and ectomycorrhizal (ECM) associations, respond to different phosphorus forms under field conditions. An in situ root bag experiment was conducted using four phosphorus treatments (control, inorganic, organic, and mixed phosphorus) across four subtropical tree species. A comprehensive set of fine root traits, including morphological, physiological, and mycorrhizal characteristics, was measured to evaluate species-specific phosphorus foraging strategies. The results showed that AM species were more responsive to phosphorus form variation than ECM species, particularly under inorganic and mixed phosphorus treatments. Significant changes in root diameter (RD), root tissue density (RTD), and acid phosphatase activity (RAP) were observed in AM species, often accompanied by higher phosphorus accumulation in fine roots. For example, RD in AM species significantly decreased under the Na3PO4 treatment (0.94 mm) compared to the control (1.18 mm), while ECM species showed no significant changes in RD across treatments (1.12–1.18 mm, p > 0.05). RTD in AM species significantly increased under Na3PO4 (0.030 g/cm3) and Mixture (0.021 g/cm3) compared to the control (0.012 g/cm3, p < 0.05), whereas ECM species exhibited consistently low RTD values across treatments (0.017–0.020 g/cm3, p > 0.05). RAP in AM species increased significantly under Na3PO4 (1812 nmol/g/h) and Mixture (1596 nmol/g/h) relative to the control (1348 nmol/g/h), while ECM species showed limited variation (1286–1550 nmol/g/h, p > 0.05). In contrast, ECM species displayed limited trait variation across treatments, reflecting a more conservative acquisition strategy. In addition, trait correlation analysis revealed stronger coordination among root traits in AM species. And AM species exhibited high variability across treatments, while ECM species maintained consistent trait distributions with limited plasticity. These findings suggest that AM and ECM species adopt fundamentally different phosphorus acquisition strategies. AM species rely on integrated morphological and physiological responses to variable phosphorus conditions, while ECM species maintain stable trait configurations, potentially supported by fungal symbiosis. Such divergence may contribute to functional complementarity and species coexistence in phosphorus-limited subtropical forests. Full article
(This article belongs to the Section Forest Ecophysiology and Biology)
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22 pages, 4695 KiB  
Article
Application of Extra-Trees Regression and Tree-Structured Parzen Estimators Optimization Algorithm to Predict Blast-Induced Mean Fragmentation Size in Open-Pit Mines
by Madalitso Mame, Shuai Huang, Chuanqi Li and Jian Zhou
Appl. Sci. 2025, 15(15), 8363; https://doi.org/10.3390/app15158363 - 28 Jul 2025
Viewed by 131
Abstract
Blasting is an effective technique for fragmenting rock in open-pit mining operations. Blasting operations produce either boulders or fine fragments, both of which increase costs and pose environmental risks. As a result, predicting the mean fragmentation size (MFS) distribution of rock is critical [...] Read more.
Blasting is an effective technique for fragmenting rock in open-pit mining operations. Blasting operations produce either boulders or fine fragments, both of which increase costs and pose environmental risks. As a result, predicting the mean fragmentation size (MFS) distribution of rock is critical for assessing blasting operations’ quality and mitigating risks. Due to the limitations of empirical and statistical models, several researchers are turning to artificial intelligence (AI)-based techniques to predict the MFS distribution of rock. Thus, this study uses three AI tree-based algorithms—extra trees (ET), gradient boosting (GB), and random forest (RF)—to predict the MFS distribution of rock. The prediction accuracy of the models is optimized utilizing the tree-structured Parzen estimators (TPEs) algorithm, which results in three models: TPE-ET, TPE-GB, and TPE-RF. The dataset used in this study was collected from the published literature and through the data augmentation of a large-scale dataset of 3740 blast samples. Among the evaluated models, the TPE-ET model exhibits the best performance with a coefficient of determination (R2), root mean squared error (RMSE), mean absolute error (MAE), and max error of 0.93, 0.04, 0.03, and 0.25 during the testing phase. Moreover, the block size (XB, m) and modulus of elasticity (E, GPa) parameters are identified as the most influential parameters for predicting the MFS distribution of rock. Lastly, an interactive web application has been developed to assist engineers with the timely prediction of MFS. The predictive model developed in this study is a reliable intelligent model because it combines high accuracy with a strong, explainable AI tool for predicting MFS. Full article
(This article belongs to the Section Computing and Artificial Intelligence)
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18 pages, 2429 KiB  
Article
Conserved and Specific Root-Associated Microbiome Reveals Close Correlation Between Fungal Community and Growth Traits of Multiple Chinese Fir Genotypes
by Xuan Chen, Zhanling Wang, Wenjun Du, Junhao Zhang, Yuxin Liu, Liang Hong, Qingao Wang, Chuifan Zhou, Pengfei Wu, Xiangqing Ma and Kai Wang
Microorganisms 2025, 13(8), 1741; https://doi.org/10.3390/microorganisms13081741 - 25 Jul 2025
Viewed by 259
Abstract
Plant microbiomes are vital for the growth and health of their host. Tree-associated microbiomes are shaped by multiple factors, of which the host is one of the key determinants. Whether different host genotypes affect the structure and diversity of the tissue-associated microbiome and [...] Read more.
Plant microbiomes are vital for the growth and health of their host. Tree-associated microbiomes are shaped by multiple factors, of which the host is one of the key determinants. Whether different host genotypes affect the structure and diversity of the tissue-associated microbiome and how specific taxa enriched in different tree tissues are not yet well illustrated. Chinese fir (Cunninghamia lanceolata) is an important tree species for both economy and ecosystem in the subtropical regions of Asia. In this study, we investigated the tissue-specific fungal community structure and diversity of nine different Chinese fir genotypes (39 years) grown in the same field. With non-metric multidimensional scaling (NMDS) analysis, we revealed the divergence of the fungal community from rhizosphere soil (RS), fine roots (FRs), and thick roots (TRs). Through analysis with α-diversity metrics (Chao1, Shannon, Pielou, ACE, Good‘s coverage, PD-tree, Simpson, Sob), we confirmed the significant difference of the fungal community in RS, FR, and TR samples. Yet, the overall fungal community difference was not observed among nine genotypes for the same tissues (RS, FR, TR). The most abundant fungal genera were Russula in RS, Scytinostroma in FR, and Subulicystidium in TR. Functional prediction with FUNGuild analysis suggested that ectomycorrhizal fungi were commonly enriched in rhizosphere soil, while saprotroph–parasite and potentially pathogenic fungi were more abundant in root samples. Specifically, genotype N104 holds less ectomycorrhizal and pathogenic fungi in all tissues (RS, FR, TR) compared to other genotypes. Additionally, significant correlations of several endophytic fungal taxa (Scytinostroma, Neonothopanus, Lachnum) with the growth traits (tree height, diameter, stand volume) were observed. This addresses that the interaction between tree roots and the fungal community is a reflection of tree growth, supporting the “trade-off” hypothesis between growth and defense in forest trees. In summary, we revealed tissue-specific, as well as host genotype-specific and genotype-common characters of the structure and functions of their fungal communities. Full article
(This article belongs to the Special Issue Rhizosphere Microbial Community, 4th Edition)
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24 pages, 3365 KiB  
Article
Energy Demand Forecasting Scenarios for Buildings Using Six AI Models
by Khaled M. Salem, Francisco J. Rey-Martínez, A. O. Elgharib and Javier M. Rey-Hernández
Appl. Sci. 2025, 15(15), 8238; https://doi.org/10.3390/app15158238 - 24 Jul 2025
Viewed by 226
Abstract
Understanding and forecasting energy consumption patterns is crucial for improving energy efficiency and human well-being, especially in diverse infrastructures like Spain. This research addresses a significant gap in energy demand forecasting across three building types by comparing six machine learning algorithms: Artificial Neural [...] Read more.
Understanding and forecasting energy consumption patterns is crucial for improving energy efficiency and human well-being, especially in diverse infrastructures like Spain. This research addresses a significant gap in energy demand forecasting across three building types by comparing six machine learning algorithms: Artificial Neural Networks, Random Forest, XGBoost, Radial Basis Function Network, Autoencoder, and Decision Trees. The primary aim is to identify the most effective model for predicting energy consumption based on historical data, contributing to the relationship between energy systems and urban well-being. The study emphasizes challenges in energy use and advocates for sustainable management practices. By forecasting energy demand over the next three years using linear regression, it provides actionable insights for energy providers, enhancing resilience in urban environments impacted by climate change. The findings deepen our understanding of energy dynamics across various building types and promote a sustainable energy future. Stakeholders will receive targeted recommendations for aligning energy production with consumption trends while meeting environmental responsibilities. Model performance is rigorously evaluated using metrics like Squared Mean Root Percentage Error (RMSPE) and Coefficient of Determination (R2), ensuring robust analysis. Training times for models in the LUCIA building ranged from 2 to 19 s, with the Decision Tree model showing the shortest times, highlighting the need to balance computational efficiency with model performance. Full article
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20 pages, 2737 KiB  
Technical Note
Obtaining the Highest Quality from a Low-Cost Mobile Scanner: A Comparison of Several Pipelines with a New Scanning Device
by Marek Hrdina, Juan Alberto Molina-Valero, Karel Kuželka, Shinichi Tatsumi, Keiji Yamaguchi, Zlatica Melichová, Martin Mokroš and Peter Surový
Remote Sens. 2025, 17(15), 2564; https://doi.org/10.3390/rs17152564 - 23 Jul 2025
Viewed by 199
Abstract
The accurate measurement of the tree diameter is vital for forest inventories, urban tree quality assessments, the management of roadside and railway vegetation, and various other applications. It also plays a crucial role in evaluating tree growth dynamics, which are closely linked to [...] Read more.
The accurate measurement of the tree diameter is vital for forest inventories, urban tree quality assessments, the management of roadside and railway vegetation, and various other applications. It also plays a crucial role in evaluating tree growth dynamics, which are closely linked to tree health, structural stability, and vulnerability. Although a range of devices and methodologies are currently under investigation, the widespread adoption of laser scanners remains constrained by their high cost. This study therefore aimed to compare high-end laser scanners (Trimble TX8 and GeoSLAM ZEB Horizon) with cost-effective alternatives, represented by the Apple iPhone 14 Pro and the LA03 scanner developed by mapry Co., Ltd. (Tamba, Japan). It further sought to evaluate the feasibility of employing these more affordable devices, even for small-scale forest owners or managers. Given the growing availability of 3D-based forest inventory algorithms, a selection of such processing pipelines was used to assess the practical potential of the scanning devices. The tested low-cost device produced moderate results, achieving a tree detection rate of up to 78% and a relative root mean square error (rRMSE) of 19.7% in diameter at breast height (DBH) estimation. However, performance varied depending on the algorithms applied. In contrast, the high-end mobile laser scanning (MLS) and terrestrial laser scanning (TLS) systems outperformed the low-cost alternative across all metrics, with tree detection rates reaching up to 99% and DBH estimation rRMSEs as low as 5%. Nevertheless, the low-cost device may still be suitable for scanning small sample plots at a reduced cost and could potentially be deployed in larger quantities to support broader forest inventory initiatives. Full article
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19 pages, 1247 KiB  
Article
Niche Overlap in Forest Tree Species Precludes a Positive Diversity–Productivity Relationship
by Kliffi M. S. Blackstone, Gordon G. McNickle, Morgan V. Ritzi, Taylor M. Nelson, Brady S. Hardiman, Madeline S. Montague, Douglass F. Jacobs and John J. Couture
Plants 2025, 14(15), 2271; https://doi.org/10.3390/plants14152271 - 23 Jul 2025
Viewed by 222
Abstract
Niche complementarity is suggested to be a main driver of productivity overyielding in diverse environments due to enhanced resource use efficiency and reduced competition. Here, we combined multiple different approaches to demonstrate that niche overlap is the most likely cause to explain a [...] Read more.
Niche complementarity is suggested to be a main driver of productivity overyielding in diverse environments due to enhanced resource use efficiency and reduced competition. Here, we combined multiple different approaches to demonstrate that niche overlap is the most likely cause to explain a lack of overyielding of three tree species when grown in different species combinations. First, in an experimental planting we found no relationship between productivity and species diversity for leaf, wood, or root production (no slope was significantly different from zero), suggesting a lack of niche differences among species. Second, data extracted from the United States Department of Agriculture Forest Inventory and Analysis revealed that the species do not significantly co-occur in natural stands (p = 0.4065) as would be expected if coexistence was common across their entire range. Third, we compared trait differences among our species and found that they are not significantly different in multi-dimensional trait space (p = 0.1724). By combining multiple analytical approaches, we provide evidence of potential niche overlap that precludes coexistence and a positive diversity–productivity relationship between these three tree species. Full article
(This article belongs to the Section Plant Ecology)
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22 pages, 4318 KiB  
Article
The Molecular Mechanism and Effects of Root Pruning Treatment on Blueberry Tree Growth
by Liwei Chu, Chengjing Shi, Xin Wang, Benyin Li, Siyu Zuo, Qixuan Li, Jiarui Han, Hexin Wang and Xin Lou
Plants 2025, 14(15), 2269; https://doi.org/10.3390/plants14152269 - 23 Jul 2025
Viewed by 173
Abstract
Root pruning can promote the transplanting of young green plants, but the overall impact of pruning on root growth, morphology, and physiological functions remains unclear. This study integrated transcriptomics and physiological analyses to elucidate the effects of root pruning on blueberry growth. Appropriate [...] Read more.
Root pruning can promote the transplanting of young green plants, but the overall impact of pruning on root growth, morphology, and physiological functions remains unclear. This study integrated transcriptomics and physiological analyses to elucidate the effects of root pruning on blueberry growth. Appropriate pruning (CT4) significantly promoted plant growth, with above-ground biomass and leaf biomass significantly increasing compared to the control group within 42 days. Photosynthesis temporarily decreased at 7 days but recovered at 21 and 42 days. Transcriptomics analysis showed that the cellulose metabolism pathway was rapidly activated and influenced multiple key genes in the starch metabolism pathway. Importantly, transcription factors associated with vascular development were also significantly increased at 7, 21, and 42 days after root pruning, indicating their role in regulating vascular differentiation. Enhanced aboveground growth was positively correlated with the expression of photosynthesis-related genes, and the transport of photosynthetic products via vascular tissues provided a carbon source for root development. Thus, root development is closely related to leaf photosynthesis, and changes in gene expression associated with vascular tissue development directly influence root development, ultimately ensuring coordinated growth between aboveground and belowground parts. These findings provide a theoretical basis for optimizing root pruning strategies to enhance blueberry growth and yield. Full article
(This article belongs to the Section Plant Physiology and Metabolism)
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17 pages, 2163 KiB  
Article
Allometric Growth of Annual Pinus yunnanensis After Decapitation Under Different Shading Levels
by Pengrui Wang, Chiyu Zhou, Boning Yang, Jiangfei Li, Yulan Xu and Nianhui Cai
Plants 2025, 14(15), 2251; https://doi.org/10.3390/plants14152251 - 22 Jul 2025
Viewed by 236
Abstract
Pinus yunnanensis, a native tree species in southwest China, is shading-tolerant and ecologically significant. Light has a critical impact on plant physiology, and decapitation improves canopy light penetration and utilization efficiency. The study of allometric relationships is well-known in forestry, forest ecology, [...] Read more.
Pinus yunnanensis, a native tree species in southwest China, is shading-tolerant and ecologically significant. Light has a critical impact on plant physiology, and decapitation improves canopy light penetration and utilization efficiency. The study of allometric relationships is well-known in forestry, forest ecology, and related fields. Under control (full daylight exposure, 0% shading), L1 (partial shading, 25% shading), L2 (medium shading, 50% shading), and L3 (serious shading, 75% shading) levels, this study used the decapitation method. The results confirmed the effectiveness of decapitation in annual P. yunnanensis and showed that the main stem maintained isometric growth in all shading treatments, accounting for 26.8% of the individual plant biomass, and exhibited dominance in biomass allocation and high shading sensitivity. These results also showed that lateral roots exhibited a substantial biomass proportion of 12.8% and maintained more than 0.5 of higher plasticity indices across most treatments. Moreover, the lateral root exhibited both the lowest slope in 0.5817 and the highest significance (p = 0.023), transitioning from isometric to allometric growth under L1 shading treatment. Importantly, there was a positive correlation between the biomass allocation of an individual plant and that of all components of annual P. yunnanensis. In addition, the synchronized allocation between main roots and lateral branches, as well as between main stems and lateral roots, suggested functional integration between corresponding belowground and aboveground structures to maintain balanced resource acquisition and architectural stability. At the same time, it has been proved that the growth of lateral roots can be accelerated through decapitation. Important scientific implications for annual P. yunnanensis management were derived from these shading experiments on allometric growth. Full article
(This article belongs to the Special Issue Development of Woody Plants)
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16 pages, 3669 KiB  
Article
Functional Analysis of Malus halliana WRKY69 Transcription Factor (TF) Under Iron (Fe) Deficiency Stress
by Hongjia Luo, Wenqing Liu, Xiaoya Wang and Yanxiu Wang
Curr. Issues Mol. Biol. 2025, 47(7), 576; https://doi.org/10.3390/cimb47070576 - 21 Jul 2025
Viewed by 210
Abstract
Fe deficiency in apple trees can lead to leaf chlorosis and impede root development, resulting in significant alterations in signaling, metabolism, and genetic functions, which severely restricts fruit yield and quality. It is well established that WRKY transcription factors (TFs) are of vital [...] Read more.
Fe deficiency in apple trees can lead to leaf chlorosis and impede root development, resulting in significant alterations in signaling, metabolism, and genetic functions, which severely restricts fruit yield and quality. It is well established that WRKY transcription factors (TFs) are of vital significance in mediating plant responses to abiotic stress. Real-time quantitative fluorescence (RT-qPCR) analysis displayed that Fe deficiency stress can significantly induce WRKY69 TF gene expression. However, the potential mechanisms by which the WRKY69 gene involved in Fe deficiency stress remains to be investigated. To address this limitations, the WRKY69 gene (MD09G1235100) was successfully isolated from apple rootstock Malus halliana and performed both homologous and heterologous expression analyses in apple calli and tobacco to elucidate its functional role in response to Fe deficiency stress. The findings indicated that transgenic tobacco plants exhibited enhanced growth vigor and reduced chlorosis when subjected to Fe deficiency stress compared to the wild type (WT). Additionally, the apple calli that were overexpressed WRKY69 also exhibited superior growth and quality. Furthermore, the overexpression of the WRKY69 gene enhanced the ability of tobacco to Fe deficiency stress tolerance by stimulating the synthesis of photosynthetic pigments, increasing antioxidant enzyme activity, and facilitating Fe reduction. Additionally, it increased the resistance of apple calli to Fe deficiency stress by enhancing Fe reduction and elevating the activity of antioxidant enzymes. For example, under Fe deficiency stress, the proline (Pro) contents of the overexpression lines (OE-2, OE-5, OE-6) were 26.18 mg·g−1, 26.13 mg·g−1, and 26.27 mg·g−1, respectively, which were 16.98%, 16.76%, and 17.38% higher than the proline content of 22.38 mg·g−1 in the wild-type lines, respectively. To summarize, a functional analysis of tobacco plants and apple calli displayed that WRKY69 TF serves as a positive regulator under Fe deficiency stress, which provides candidate genetic resources for cultivating apple rootstocks or varieties with strong stress (Fe deficiency) resistance. Full article
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21 pages, 8521 KiB  
Article
Estimating Forest Carbon Stock Using Enhanced ResNet and Sentinel-2 Imagery
by Jintong Ren, Lizhi Liu, You Wu, Lijian Ouyang and Zhenyu Yu
Forests 2025, 16(7), 1198; https://doi.org/10.3390/f16071198 - 20 Jul 2025
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Abstract
Accurate estimation of forest carbon stock is critical for understanding ecosystem carbon dynamics and informing climate mitigation strategies. This study presents a deep learning framework that integrates Sentinel-2 multispectral imagery with an enhanced residual neural network for estimating aboveground forest carbon stock in [...] Read more.
Accurate estimation of forest carbon stock is critical for understanding ecosystem carbon dynamics and informing climate mitigation strategies. This study presents a deep learning framework that integrates Sentinel-2 multispectral imagery with an enhanced residual neural network for estimating aboveground forest carbon stock in the Liuchong River Basin, Bijie City, Guizhou Province, China. The proposed model incorporates multiscale residual blocks and channel attention mechanisms to improve spatial feature extraction and spectral dependency modeling. A dataset of 150 ground inventory plots was employed for supervised training and validation. Comparative experiments with Random Forest, Gradient Boosting Decision Trees (GBDT), and Vision Transformer (ViT) demonstrate that the enhanced ResNet achieves the best performance, with a root mean square error (RMSE) of 23.02 Mg/ha and a coefficient of determination (R2) of 0.773 on the test set. Spatial mapping results further reveal that the model effectively captures fine-scale carbon stock variations across mountainous forested landscapes. These findings underscore the potential of combining multispectral remote sensing and advanced neural architectures for scalable, high-resolution forest carbon estimation in complex terrain. Full article
(This article belongs to the Special Issue Mapping and Modeling Forests Using Geospatial Technologies)
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