Search Results (97)

Open-field burning of agricultural residues is a persistent environmental challenge that contributes to greenhouse gas emissions, air pollution, and the loss of valuable biomass resources. This study developed sustainable mushroom cultivation substrates by replacing 50% of conventional sawdust with locally available agricultural residues, including corn stalks, rice straw, sugarcane leaves, and leaf litter, to reduce production costs, enhance mushroom productivity, and promote circular bioeconomy practices. The physicochemical properties, mycelial growth, contamination, yield performance, nutritional composition, economic feasibility, and environmental benefits of the alternative substrates were evaluated using Lentinus sajor-caju and Pleurotus species. Among the tested formulations, corn stalk-based substrates exhibited the most favorable characteristics, with improved nitrogen availability and a more balanced C/N ratio, resulting in faster colonization, lower contamination, quicker fruiting body formation period, and superior biological efficiency. The corn stalk formulation achieved the highest productivity across species, including biological efficiencies of 61.99% for L. sajor-caju, 102.79% for P. cornucopiae, 99.40% for P. ostreatus, and 101.96% for P. pulmonarius. In addition, alternative substrates maintained or enhanced mushroom nutritional quality, with high protein (18.22–31.37%), dietary fiber (up to 30.58%), and low-fat contents (1.11–1.95%). Economic analysis demonstrated that a 50:50 sawdust-biomass substitution strategy substantially reduced substrate costs, achieving approximately 30% savings at industrial production scales. Overall, this study demonstrates that converting agricultural residues into high-value mushroom substrates provides an effective strategy to improve production efficiency, reduce costs, and advance sustainable mushroom cultivation systems. Full article

The growing demand for low-carbon building materials and the challenges of handling agroforestry waste and discarded EPS particles have spurred research toward developing novel building composites that utilize solid waste. Therefore, this study aims to develop a lightweight geopolymer composite incorporating these recycled materials to balance thermal insulation, mechanical strength, and waterproofing properties. In this work, geopolymer served as the binder, with various types of raw biomass (sawdust, rice husk, rice straw, and coconut fiber) as the primary aggregates and EPS particles as an additive to create a closed-pore structure. The microstructure of the raw biomass was characterized by SEM, while its specific surface area and average pore diameter were determined by BET analysis. Furthermore, the prepared composites were comprehensively evaluated in terms of their microstructure, pore structure (MIP), density, thermal conductivity, compressive strength, total water absorption, capillary water absorption, surface wettability, and UV aging behavior. The results showed that the prepared composites exhibited a porosity of 59.9–65.7%, a density of 492.9–586.3 kg/m³, a compressive strength of 7.3–10.9 MPa, a thermal conductivity of 0.115–0.142 W/(m·K), a total water absorption of 35.2–42.2%, capillary water uptake coefficients of 4.9–11 kg/m², and a water contact angle exceeding 140° (after modification). In addition, the developed composites offered significant environmental and economic benefits, with a low carbon footprint and an estimated cost of 100.6–150.3 USD/m³, making them more competitive compared to traditional insulation materials. Meanwhile, this study provides a scientific basis for developing high-strength building insulation materials from agroforestry waste, thus outlining a promising direction for future research and industry development. Full article

A three-factor completely randomized design was used to evaluate the effects of Lactococcus lactis, Bacillus subtilis, and Saccharomyces cerevisiae on the fermentation quality and nutritional composition of mixed silage prepared from navel orange pomace and rice straw. The addition of L. lactis increased lactic acid content and Flieg’s score of the mixed silage. The addition of B. subtilis decreased the pH value and increased the lactic acid content of the mixed silage. The addition of S. cerevisiae decreased the lactic acid content and Flieg’s score of the mixed silage. However, the interaction between the three strains significantly affected many parameters of the mixed silage. With respect to fermentation quality (Flieg’s score), adding 0.3 g kg⁻¹ L. lactis and not adding S. cerevisiae achieved the best performance of the mixed silage. Under these conditions, increasing the level of B. subtilis resulted in a higher crude protein content of the mixed silage, whereas neutral detergent fiber and acid detergent fiber contents first decreased and then increased, and water-soluble carbohydrate content first increased and then decreased. Therefore, within the selected combination of adding 0.3 g kg⁻¹ L. lactis and not adding S. cerevisiae, the addition of 0.8 g kg⁻¹ B. subtilis gave the best nutritional quality. Full article

Rice straw, one of the most abundant agricultural residues worldwide, remains significantly underutilized as a ruminant feed source owing to its intrinsic lignocellulosic recalcitrance. This study investigated the effects of co-extruding rice straw with varying proportions of corn flour on nutritional composition and in vitro digestibility for ruminant nutrition. Extrusion was conducted using a twin-screw extruder at 180 °C barrel temperature, 5 MPa pressure, and 50% feed moisture content. Five corn levels were formulated on a dry matter basis: pure rice straw (RS100); three blends with increasing corn flour inclusion: RS75:C25 (75% straw + 25% corn flour), RS67:C33 (67% straw + 33% corn flour), and RS60:C40 (60% straw + 40% corn flour); and pure corn flour (C100) as a control. Chemical composition including neutral detergent fiber (NDF), acid detergent fiber (ADF), acid detergent lignin (ADL), cellulose, hemicellulose, water-soluble carbohydrates (WSC), and starch was analyzed. In vitro dry matter digestibility (IVDMD) was determined using a pepsin-cellulase assay. Regression analysis within the practical 0–40% corn flour inclusion range revealed a significant quadratic relationship with IVDMD (R² = 0.999, p < 0.001). The optimal corn flour proportion was calculated to be approximately 37.5%, which closely matched the RS60:C40 formulation (40% corn flour). Among the tested formulations, RS60:C40 exhibited the greatest extrusion-induced nutritional improvements. Relative to its pre-extrusion values, cellulose decreased by 55.7% (p < 0.05), followed by ADF (16.1%), NDF (12.8%), and hemicellulose (10.2%); IVDMD increased by 34.2% (p < 0.01) and WSC by 56.7% (p < 0.05). Compared with RS100 after extrusion, RS60:C40 raised IVDMD by 49.5% and lowered cellulose by 60.6%. Its IVDMD also surpassed those of RS75:C25 and RS67:C33 (p < 0.05), whereas RS75:C25 showed only marginal improvements. ADL content showed no extrusion-induced change (p > 0.05). Scanning electron microscopy (SEM) of the RS60:C40 formulation revealed that, unlike the intact fibrous structures observed prior to extrusion, post-extrusion samples exhibited extensive disruption of the fibrous matrix. Pearson correlation analysis further supported these findings, showing strong positive correlations between IVDMD and WSC (r = 0.96, p < 0.001) and strong negative correlations between IVDMD and NDF (r = −0.95, p < 0.001). In conclusion, extrusion generally increased IVDMD and WSC while reducing fiber fractions, with the effect depending on corn level. Co-extrusion with 40% corn flour effectively enhanced the nutritional value of rice straw, offering a viable strategy for producing a more digestible ruminant feed. Full article

Rice (Oryza sativa L.) straw-return can improve soil carbon (C) sequestration, but its adoption in intensive rice systems is limited by short fallow periods (<30 days), which likely lead to incomplete straw decomposition and increase methane emissions under continuous flooding (CF). Brittle rice straw, characterized by lower recalcitrant fiber content and rapid decomposition, may overcome this constraint; however, its environmental performance under alternate wetting and drying (AWD) remains unclear, such as broader C allocation. This 150-day microcosm study evaluated the interaction of straw type (brittle vs. non-brittle) and water management (CF vs. AWD) on greenhouse gas (GHG) emissions, dissolved C production, soil C storage, and aggregate formation in two contrasting paddy soils (sandy loam vs. silty clay loam). Compared with non-brittle straw, brittle straw returns reduced net GHG emissions by approximately 28.4% under CF and 39.6% under AWD. The combination of brittle straw with AWD produced the lowest net GHG emissions (0.61 kg CO₂-eq m⁻²), indicating that intermittent oxygen input effectively mitigated the early decomposition-related emission risk. Brittle straw also increased the concentrations of dissolved inorganic C by 14.2% and nitrate by 64.3% under AWD, suggesting enhanced mineralization and potential inorganic C stabilization. Regardless of straw type, straw return improved soil C stocks by 27.3% in sandy loam and 29.6% in silty clay loam, while also promoting macroaggregate formation. Overall, this study demonstrated that coupling brittle rice straw with AWD can reduce GHG emissions while maintaining soil C benefits, offering a promising residue management strategy for intensive rice cultivation. Full article

Driven by global environmental regulations that strictly limit copper content in brake pads, traditional copper-based friction materials face significant challenges due to their negative ecological impacts. Consequently, the development of sustainable, copper-free alternatives has become an inevitable trend in the braking industry. This study proposes a novel approach to developing high-performance green friction materials by utilizing a synergistic combination of agricultural wastes, specifically corn cobs, wheat straw, rice husks, and sugarcane bagasse, and carbon fibers. Research indicates that the friction coefficient of the synergistic formulation remains stable within the range of 0.35 to 0.48. Compared with the control group, this formulation achieves an average reduction in the wear rate of 19.28% and an increase in the recovery rate of 5.15%, demonstrating superior tribological performance. The synergistic interfacial regulation between carbon fibers and agricultural waste facilitates the construction of a smooth and stable friction layer, which maintains consistent performance during extended operating conditions. Among all formulations investigated, the composite reinforced by the synergy of corncob and carbon fiber exhibits the most prominent comprehensive properties, with the wear rate decreasing by 28.73% and the recovery performance improving by 4.05% relative to the specimen containing copper fibers. This work not only provides a new pathway for the sustainable development of green friction materials but also offers a theoretical basis for the high-value utilization of agricultural waste resources. Full article

The modeling accuracy of rice straw remains limited, and discrete element method (DEM) simulations of its compression are computationally intensive. To address these challenges, this study systematically investigated the physical characteristics of rice straw and proposed an innovative DEM and parameter calibration approach. Uniaxial compression tests were conducted on individual straw stalks, and key DEM parameters were systematically calibrated using Plackett–Burman experiments, steepest ascent trials, and Central Composite design. The calibrated parameters were validated against single-straw compression tests, showing a relative error of only 1.9% between simulated and measured peak loads, indicating high model fidelity. Building on this foundation, vibration-assisted compression bench tests were performed on bulk straw, further validating the scaled-up DEM and its parameters. The evolution of normal forces and porosity during compression was analyzed by comparing experimental results with simulations, confirming the model’s accuracy in capturing straw compaction behavior. Finally, a comparison of computational efficiency between the scaled-up and original DEMs revealed that the scaled-up model reduced computation time by approximately 67.4% and 65.2%, respectively, significantly improving simulation efficiency. This study provides a robust methodology for modeling flexible agricultural fibers and establishes a foundation for efficient numerical simulation of straw compression. Full article

In ruminant nutrition, the lignocellulosic complex is a primary constraint limiting the utilization of dietary fiber. The objective of this study was to evaluate the effects of inoculating lignin-degrading bacteria (LDB) isolated from the ruminant rectum on in vitro rumen fermentation characteristics. Rectal fecal samples were collected from healthy beef cattle, dairy cattle, buffaloes, and goats (n = 4 per species) using the grab sampling technique. Twenty-eight bacterial colonies were isolated through enrichment and screening on media containing sodium lignosulfonate. Lignin degradation efficiency was assessed spectrophotometrically, while laccase activity was determined using a 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) oxidation assay. Seven isolates exhibiting ligninolytic activity (1.4–5.6% degradation efficiency) were selected to evaluate their effects on in vitro rumen fermentation using a completely randomized design with four replicates. LDB treatments were standardized to a concentration of 2.4 × 10⁵ colony-forming units/mL of rumen fluid medium, while the control received an equal volume of a 0.85% sterile NaCl solution. A rice straw-based total mixed ration served as the substrate, with rumen fluid collected from beef cattle. All treatments were incubated for 48 h. Notably, isolate BC3 consistently enhanced in vitro dry matter digestibility (23.1%), total gas production (18.6%), and total volatile fatty acid concentrations (13.2%) relative to the control and other LDB isolates (p < 0.01). All seven LDB isolates were identified as Gram-negative, rod-shaped, facultative anaerobic bacteria that exhibit catalase activity and tolerate moderately acidic conditions. Phylogenetic tree analysis based on 16S rRNA gene sequencing identified isolate BC3 as being closely related to Escherichia coli strains. These findings demonstrate that the ruminant hindgut is a promising source of LDB with the functional potential to enhance feed digestibility and fermentation end-products in the rumen. Future research should prioritize in vivo trials to evaluate the safety and efficacy of LDB as a direct-fed microbial, specifically focusing on its impact on animal performance and health. Full article

We report on the isolation and comprehensive genomic and biochemical characterization of Aspergillus fumigatus VP2T, a thermophilic filamentous fungus recovered from Himalayan Forest soil with exceptional lignocellulolytic capacity. Whole-genome sequencing revealed a 32.1 Mb genome encoding 12,675 predicted genes, including an extensive repertoire of >300 carbohydrate-active enzymes (CAZymes). Notably, the genome harbors multiple auxiliary activity enzymes, including AA9-family lytic polysaccharide monooxygenases and several cellobiose dehydrogenases (CDHs), supporting oxidative–hydrolytic synergism during biomass degradation. Submerged fermentation using a cellulose–wheat bran–rice straw substrate induced high enzyme titers, including 33 U/mL endoglucanase and 131 U/mL CDH, exceeding activities commonly reported for both native and engineered fungal strains. Although exoglucanase (0.02 U/mL) and xylanase (14.22 U/mL) activities were comparatively modest, the strain VP2T demonstrated superior hydrolysis of untreated rice straw, achieving a 1.89-fold increase in saccharification efficiency relative to the commercial enzyme cocktail Cellic^® CTec2. Scanning electron microscopy confirmed extensive disruption of lignocellulosic architecture, consistent with enhanced enzyme accessibility and oxidative fiber loosening. Collectively, genomic evidence and functional assays identify A. fumigatus VP2T as a redox-optimized, moderately thermophilic biocatalyst suited for low-pH lignocellulose conversion. This study highlights the value of exploring thermophilic fungal biodiversity to discover native strains with inherent oxidative capacity, offering promising alternatives to pretreatment-intensive biorefinery processes and informing the rational development of tailored enzyme systems. Full article

This experiment evaluated the application effects of the dietary substitution of maize silage with mixed silage prepared with Pennisetum giganteum and rice straw on fattening lambs. Forty-eight male Hu lambs with similar body weights and ages were randomly divided into four groups. The maize silage in the diet was replaced with Pennisetum giganteum and rice straw mixed silage in proportions of 0 (CON), 25% (PR1), 50% (PR2) and 75% (PR3). The average daily gain of the PR3 group was lower (p < 0.05) than that of the other groups. The highest substitution level increased (p < 0.05) ruminal ammonia nitrogen concentration and acetate-to-propionate ratio in lambs compared with the CON and PR1 groups. Moreover, dry matter and neutral detergent fiber digestibility in PR3 lambs were lower (p < 0.05) than in PR1 lambs. Compared with the CON group, the concentrations of serum catalase and total antioxidant capacity were increased (p < 0.05) in the PR2 and PR3 groups. Overall, the dietary substitution of maize silage with Pennisetum giganteum and rice straw mixed silage at a 50% level did not show a negative influence on growth performance of fattening lambs but displayed positive effects on their fiber digestibility and antioxidative capacity. Full article

In this study, eight treatment groups were set up with three replicates in each group to investigate the effects of Bacillus, Lactobacillus, and molasses on the chemical composition and fermentation quality of fermented rice straw. Furthermore, an animal experiment was conducted to determine the nutrition apparent digestibility, ruminal fermentation characteristics, and methane emission in Hu sheep. The results showed that the dry matter (DM) and crude protein (CP) content in the group treated with Bacillus megaterium and Lactobacillus acidophilus (BMLB) was significantly higher than that in the group with no additive (CK) (p < 0.05). Compared with the CK group, all treatments with single or composite addition of Bacillus significantly reduced the content of neutral detergent fiber (NDF) and acid detergent fiber (ADF) (p < 0.05). The content of acetate, propionate, and NH₃-N in the BMLB group were significantly lower than those in the CK group (p < 0.05). In addition, the Hu sheep in the BMLB group showed a significant reduction in daily methane emission per unit of metabolic body weight. In conclusion, the BMLB treatment significantly improved the nutritional value, fermentation quality, ruminal fermentation, and methane emission of rice straw haylage in Hu sheep. Full article

The incorporation of agricultural waste into construction materials represents a promising pathway toward achieving carbon neutrality in the building sector. This study investigates the flexural performance of a novel prefabricated external wall panel composed of corn straw concrete (CSC), an eco-friendly composite material that utilizes waste corn straws. While prior studies have explored rice straw and hemp fiber concrete, they primarily focused on the mechanical properties of these materials rather than the design of prefabricated panels. This study fills the gap by optimizing reinforcement ratio and window opening layout for CSC panels, and validating their structural viability for prefabricated enclosures. An optimal mix proportion was identified, which meets the mechanical requirements for non-load-bearing applications. Four prototype panel specimens were subjected to out-of-plane monotonic loading, considering variables including reinforcement ratio (0.18% vs. 0.24%) and the presence of a window opening (25% area ratio). Results indicated that increasing the reinforcement ratio significantly enhanced the ultimate load capacity by up to 33.3% (from 45 kN to 60 kN)—an enhancement effect that was 12–15% higher than that of reported rice straw concrete. In contrast, the introduction of an opening reduced the ultimate load capacity by 11.1–16.7%. A detailed nonlinear finite element model (FEM) was developed and validated against experimental results. The validation results indicated deflection error of 7.7–12.8% (mean: 9.33%; SD: 2.05), ultimate load error of 7.7–11.1% (mean: 9.48%; SD: 1.32), and a correlation coefficient (R²) of 0.96 between simulated and experimental values. Furthermore, analytical methods for predicting the cracking moment (with an average error of 5.97%) and ultimate flexural capacity, based on yield line theory (with an average error of 8.43%), were proposed and verified. This study demonstrates the structural viability of CSC panels and provides a sustainable solution for waste reduction in prefabricated building enclosures, contributing to greener construction practices. Full article

The frequent blasting in underground mines results in stress waves of different intensities, which is one of the main factors leading to backfill collapse. Improving the strength of backfill is an effective way to reduce the backfill damage. In this study, rice straw fiber and graded tailings were used as raw materials to prepare rice straw fiber-reinforced cemented tailings backfill (RSCTB). An orthogonal experimental design was employed to perform unconfined compressive strength (UCS) tests, diffusivity measurements, and Split Hopkinson Pressure Bar (SHPB) tests. The results showed that straw fibers slightly reduce slurry fluidity. The UCS of RSCTB at a specific mix ratio was more than 50% higher than that of cemented tailings backfill (CTB) without rice straw. The dynamic unconfined compressive strength (DUCS) of RSCTB increased linearly at different strain rates. The effect of rice straw fibers on the UCS and DUCS was much smaller than that of cement content and solid mass concentration. Excessively long and abundant straw fibers are not conducive to improving the long-term impact resistance of RSCTB. Full article

To achieve dual functionality that can monitor both Al³⁺ levels in food and the freshness of fish, rice straw fibers (RSFs) were treated in NaOH solutions and then cationized with 2,3-epoxypropyltrimethylammonium chloride, onto which alizarin red S molecules were immobilized through electrostatic interaction to develop a smart felt-like label. An optimized treatment in 5 wt% NaOH solution effectively removed lignin and hemicellulose, facilitating quaternary ammonium group grafting and stable ARS anchoring. The ARS@BRSF-5NaOH exhibited high pH sensitivity, showing visually discernible color changes (ΔE > 5, perceptible to the naked eye) under acidic (pH ≤ 6) and strongly alkaline (pH > 12) conditions. During the storage of the fish, the label transformed from yellow to dark purple (ΔE increase) as TVB-N levels approached 20 mg/100 g, enabling real-time freshness monitoring for protein-rich products. Additionally, the label achieved a detection threshold of 1 × 10⁻⁵ mol·L⁻¹ for Al³⁺ through a coordination-induced chromatic transition (purple to pale pink). This research highlights the feasibility of utilizing an agricultural waste-derived material to develop cost-effective, visually responsive, dual-functional intelligent labels for food safety, offering significant advancements in on-site quality assessment. Full article

The efficient degradation of lignocellulose is essential for valorizing agricultural waste and reducing environmental pollution. An efficient degradation process requires an enzyme cocktail capable of comprehensively deconstructing lignocellulosic components. In this study, the secretome of Coriolopsis trogii Mafic-2001 induced by rice straw was examined, and the enzymatic composition and reaction conditions of Coriolopsis trogii were optimized. Mafic-2001 secreted an enzyme cocktail that included ligninolytic enzymes, cellulases, and hemicellulases. However, the relative abundances of endoglucanase (EG) and β-glucosidase (βG) were only 64.37% and 10.69%, respectively, compared with the relative abundance of cellobiohydrolase, which indicated a critical bottleneck in degradation efficiency. To overcome this limitation, the recombinant enzymes rEG1 and rβG1 were expressed in Pichia pastoris X-33. A functionally enhanced enzyme cocktail (rEG1–rβG1–Mafic-2001 = 0.05:0.09:0.86) was developed via a mixture design to achieve a reducing sugar yield of 2.77 mg/mL from Chinese distillers’ grains (CDGs). Structural analyses revealed that the optimized enzyme cocktail disrupted the reticulated fiber architecture of CDGs and attenuated the characteristic Fourier-transform infrared spectroscopy peaks of lignin, cellulose, and hemicellulose. This study elucidates the synergistic lignocellulose deconstruction mechanism of Mafic-2001 and establishes a precision enzyme-supplementation strategy for efficient CDG bioconversion, providing a scalable platform for the valorization of lignocellulosic biomass. Full article