Search Results (1,224)

Background: Airway management in patients with obstructive sleep apnea (OSA) presents unique challenges for anesthesiologists and other airway practitioners. This comprehensive review examines current evidence and clinical practices for managing difficult airways in this high-risk population. OSA is characterized by specific anatomical and physiological alterations that increase both the likelihood of encountering difficult intubation and the risk of rapid desaturation during airway manipulation. Methods: Preoperative assessment of OSA patients requires integration of traditional difficult airway evaluation with OSA-specific considerations, including severity indices, oxygen desaturation patterns, and continuous positive airway pressure dependency. Conventional direct laryngoscopy often proves inadequate in these patients, prompting the development and refinement of alternative approaches. Videolaryngoscopy has emerged as a particularly valuable technique in OSA patients, offering improved glottic visualization while maintaining physiologic positioning. Flexible endoscopic techniques, particularly awake flexible bronchoscopic intubation, remain essential for high-risk scenarios, though they require considerable expertise. Results: Recent technological innovations have produced hybrid devices combining multiple modalities to address the specific challenges presented by OSA patients. Adjunctive tools and techniques, including specialized introducers, exchange catheters, and high-flow nasal oxygen, play critical roles in extending safe apnea time and facilitating successful intubation. Professional society guidelines now incorporate OSA-specific recommendations, emphasizing thorough preparation, appropriate device selection, and comprehensive monitoring. Conclusions: Effective management ultimately requires not only appropriate technology but also systematic preparation, strategic device selection, and meticulous execution. As OSA prevalence continues to rise globally, optimizing airway management approaches for this challenging population remains a critical priority for patient safety. Full article

Co_0.95R_0.05Fe₂O₄ nanoparticles with R = La, Pr, Nd, Sm, and Eu were synthesized via an environmentally friendly sol–gel method. The prepared samples were studied using X-ray diffraction measurements (XRD), transmission electron microscopy (TEM), X-ray photoelectron microscopy (XPS), and magnetic measurements. All compounds were found to be single phases adopting a cubic Fd-3m structure. EDS analysis confirmed the presence of Co, Fe, R, and oxygen in all cases. The XPS measurements reveal that the Co 2p core-level spectra are characteristic for Co³⁺ ions, as indicated by the 2p_3/2 and 2p_1/2 binding energies and spin–orbit splitting values. The analysis of the Fe 2p core-level spectra reveals the presence of both Fe³⁺ and Fe²⁺ ions in the investigated samples. The doped samples exhibit lower saturation magnetizations than the pristine sample. Very good agreement with the saturation magnetization values was obtained if we assumed that the light rare-earth ions occupy octahedral sites and their magnetic moments align parallel to those of the 3d transition metal ions. The ZFC-FC curves indicate that some nanoparticles remain superparamagnetic, while others exhibit ferrimagnetic ordering at room temperature, suggesting the presence of interparticle interactions. The M_r/M_s ratio at room temperature reflects the dominance of magnetostatic interactions. Full article

This study examines urban expansion, coastal erosion, and extreme wave events in Licantén, Maule Region, following the 2010 earthquake and tsunami. Using multi-source data—Landsat and Sentinel-2 imagery, ERA5 reanalysis, high-resolution Maxar images, UAV surveys, and the CoastSat algorithm—we detected significant urban growth in tsunami-prone areas: Iloca (36.88%), La Pesca (33.34%), and Pichibudi (20.78%). A 39-year shoreline reconstruction (1985–2024) revealed notable changes in erosion rates and shoreline dynamics using DSAS v6.0, influenced by tides, storm surges, and wave action modeled in R to quantify storm surge events over time. Results underscore the lack of urban planning in hazard-exposed areas and the urgent need for resilient coastal management under climate change. Full article

The accurate measurement of the tree diameter is vital for forest inventories, urban tree quality assessments, the management of roadside and railway vegetation, and various other applications. It also plays a crucial role in evaluating tree growth dynamics, which are closely linked to tree health, structural stability, and vulnerability. Although a range of devices and methodologies are currently under investigation, the widespread adoption of laser scanners remains constrained by their high cost. This study therefore aimed to compare high-end laser scanners (Trimble TX8 and GeoSLAM ZEB Horizon) with cost-effective alternatives, represented by the Apple iPhone 14 Pro and the LA03 scanner developed by mapry Co., Ltd. (Tamba, Japan). It further sought to evaluate the feasibility of employing these more affordable devices, even for small-scale forest owners or managers. Given the growing availability of 3D-based forest inventory algorithms, a selection of such processing pipelines was used to assess the practical potential of the scanning devices. The tested low-cost device produced moderate results, achieving a tree detection rate of up to 78% and a relative root mean square error (rRMSE) of 19.7% in diameter at breast height (DBH) estimation. However, performance varied depending on the algorithms applied. In contrast, the high-end mobile laser scanning (MLS) and terrestrial laser scanning (TLS) systems outperformed the low-cost alternative across all metrics, with tree detection rates reaching up to 99% and DBH estimation rRMSEs as low as 5%. Nevertheless, the low-cost device may still be suitable for scanning small sample plots at a reduced cost and could potentially be deployed in larger quantities to support broader forest inventory initiatives. Full article

Enteroendocrine cells (EECs) are specialized secretory cells in the gut epithelium that differentiate from intestinal stem cells (ISCs). Mature EECs secrete incretin hormones that stimulate pancreatic insulin secretion and regulate appetite. Decreased EEC numbers and impaired secretion of the incretin glucagon-like peptide-1 (GLP1) have been implicated in obesity-associated metabolic complications. Gut microbial metabolites of dietary tryptophan (TRP) were recently shown to modulate ISC proliferation and differentiation. However, their specific effects on EEC differentiation are not known. We hypothesized that the gut microbial metabolites of dietary tryptophan counteract impaired GLP1 production and function in obesity by stimulating EEC differentiation from ISCs. We utilized complementary models of human and rat intestines to determine the effects of obesity or TRP metabolites on EEC differentiation. EEC differentiation was assessed by the EEC marker chromogranin A (CHGA) levels in the intestinal mucosa of normal versus obese rats. The effects of TRP metabolites on EEC differentiation were determined in human intestinal organoids treated with indole, a primary TRP metabolite, or the culture supernatant of Lactobacillus acidophilus grown in TRP media (LA-CS-TRP). Our results showed that the mRNA and protein levels of CHGA, the EEC marker, were significantly decreased (~60%) in the intestinal mucosa of high-fat-diet-induced obese rat intestines. The expression of the transcription factors that direct the ISC differentiation towards the EEC lineage was also decreased in obesity. In human organoids, treatment with indole or LA-CS-TRP significantly increased (more than 2-fold) CHGA levels, which were blocked by the aryl hydrocarbon receptor (AhR) antagonist CH-223191. Thus, the stimulation of EEC differentiation by colonic microbial metabolites highlights a novel therapeutic role of TRP metabolites in obesity and associated metabolic disorders. Full article

Aerogel sponges of bio-based polymers loaded with metal–organic frameworks (MOFs) are highly promising for environmental applications, but a central challenge is to improve their stability and efficiency for removal processes. Here, the effective incorporation of the MOFs MIL-100(Fe) and ZIF-8 in composite aerogels of chitosan–pectin–lactic acid is reported. The presence of pectin was critical to loading the MOFs efficiently and homogeneously, while the incorporation of lactic acid induced a large increase in the Young’s modulus and provided structural preservation in aqueous solutions. The presence of MOFs enhanced the removal of two dyes, methyl orange (MO) and methylene blue (MB), under batch and flow conditions, with removal efficiencies of methyl orange of about 85% and 90% when loaded with ZIF-8 and MIL-100(Fe), respectively. Bentonite, celite 545, and two ionenes were loaded for comparison. Factors beyond charge-to-charge electrostatic interactions influenced the removal, since no correlations were obtained between the electrical charges of dyes, fillers, and polymers. The kinetic data were analyzed by adapting the Langmuir kinetic model, incorporating absorption and desorption processes, which allowed the recovery of the respective rate constants. Full article

The aim of translucency-enhancing liquids (TEL) is to locally influence the phase composition of zirconia in order to increase its translucency. This study aimed to determine the influence of TEL on 3Y- and 4Y-TZP zirconia concerning roughness, hardness, wear, flexural strength, dynamic stability and fracture force of fixed dental prostheses after thermal cycling and mechanical loading. Two zirconia materials (4Y-TZP; 3Y-TZP-LA, n = 8 per material and test) were investigated with and without prior application of TEL. Two-body wear tests were performed in a pneumatic pin-on-block design (50 N, 120,000 cycles, 1.6 Hz) with steatite balls (r = 1.5 mm) as antagonists. Mean and maximum vertical loss as well as roughness (Ra, Rz) were measured with a 3D laser-scanning microscope (KJ 3D, Keyence, J). Antagonist wear was determined as percent area of the projected antagonist area. Martens hardness (HM; ISO 14577-1) and biaxial flexural strength (BFS; ISO 6872) were investigated. The flexural fatigue limit BFSdyn was determined under cyclic loading in a staircase approach with a piston-on-three-ball-test. Thermal cycling and mechanical loading (TCML: 2 × 3000 × 5 °C/55 °C, 2 min/cycle, H₂O dist., 1.2 × 10⁶ force á 50 N) was performed on four-unit fixed dental prostheses (FDPs) (n = 8 per group) and the fracture force after TCML was determined. Statistics: ANOVA, Bonferroni test, Kaplan–Meier survival, Pearson correlation; α = 0.05. TEL application significantly influences roughness, hardness, biaxial flexural strength, dynamic performance, as well as fracture force after TCML in 3Y-TZP. For 4Y-TZP, a distinct influence of TEL was only identified for BFS. The application of TEL on 3Y- or 4Y-TZP did not affect wear. TEL application has a strong effect on the mechanical properties of 3Y-TZP and minor effects on 4Y-TZP. All effects of the TEL application are of a magnitude that is unlikely to restrict clinical application. Full article

Accurate estimation of erupted lava volumes is essential for understanding volcanic processes, interpreting eruptive cycles, and assessing volcanic hazards. Traditional methods based on Mid-Infrared (MIR) satellite imagery require clear-sky conditions during eruptions and are prone to sensor saturation, limiting data availability. Here, we present an alternative approach based on the post-eruptive Thermal InfraRed (TIR) signal, using the recently proposed VRP_TIR method to quantify radiative energy loss during lava flow cooling. We identify thermally anomalous pixels in VIIRS I5 scenes (11.45 µm, 375 m resolution) using the TIRVolcH algorithm, this allowing the detection of subtle thermal anomalies throughout the cooling phase, and retrieve lava flow area by fitting theoretical cooling curves to observed VRP_TIR time series. Collating a dataset of 191 mafic eruptions that occurred between 2010 and 2025 at (i) Etna and Stromboli (Italy); (ii) Piton de la Fournaise (France); (iii) Bárðarbunga, Fagradalsfjall, and Sundhnúkagígar (Iceland); (iv) Kīlauea and Mauna Loa (United States); (v) Wolf, Fernandina, and Sierra Negra (Ecuador); (vi) Nyamuragira and Nyiragongo (DRC); (vii) Fogo (Cape Verde); and (viii) La Palma (Spain), we derive a new power-law equation describing mafic lava flow thickening as a function of time across five orders of magnitude (from 0.02 Mm³ to 5.5 km³). Finally, from knowledge of areas and episode durations, we estimate erupted volumes. The method is validated against 68 eruptions with known volumes, yielding high agreement (R² = 0.947; ρ = 0.96; MAPE = 28.60%), a negligible bias (MPE = −0.85%), and uncertainties within ±50%. Application to the February-March 2025 Etna eruption further corroborates the robustness of our workflow, from which we estimate a bulk erupted volume of 4.23 ± 2.12 × 10⁶ m³, in close agreement with preliminary estimates from independent data. Beyond volume estimation, we show that VRP_TIR cooling curves follow a consistent decay pattern that aligns with established theoretical thermal models, indicating a stable conductive regime during the cooling stage. This scale-invariant pattern suggests that crustal insulation and heat transfer across a solidifying boundary govern the thermal evolution of cooling basaltic flows. Full article

Background/Objectives: The bidirectional selection of the Roman low- (RLA) and Roman high-avoidance (RHA) rat strains for extremely slow vs. very rapid acquisition of the two-way (shuttle-box) avoidance response has generated two divergent phenotypic profiles: RHA rats exhibit a behavioural pattern and gene expression profile in the frontal cortex and hippocampus (HPC) that are relevant to social and attentional/cognitive schizophrenia-linked symptoms; on the other hand, RLA rats display phenotypic traits linked to increased anxiety and sensitivity to stress-induced depression-like behaviours. The present studies aimed to evaluate the enduring and potentially positive effects of neonatal handling-stimulation (NH) on the traits differentiating these two strains of rats. Methods: We evaluated the effects of NH on anxious behaviour, prepulse inhibition of startle (PPI), spatial working memory, and hormone responses to stress in adult rats of both strains. Furthermore, given the proposed involvement of neuronal/synaptic plasticity and neurotrophic factors in the development of anxiety, stress, depression, and schizophrenia-related symptoms, using Western blot (WB) we assessed the effects of NH on the content of brain-derived neurotrophic factor (BDNF), its trkB receptor and Polysialilated-Neural Cell Adhesion Molecule (PSA-NCAM), in the prefrontal cortex (PFC), anterior cingulate cortex (ACg), ventral (vHPC), and dorsal (dHPC) hippocampus of adult rats from both strains. Results: NH increased novelty-induced exploration and reduced anxiety, particularly in RLA rats, attenuated the stress-induced increment in corticosterone and prolactin plasma levels, and improved PPI and spatial working memory in RHA rats. These effects correlated to long-lasting increases of BDNF and PSA-NCAM content in PFC, ACg, and vHPC. Conclusions: Collectively, these findings show enduring and distinct NH effects on neuroendocrine and behavioural and cognitive processes in both rat strains, which may be linked to neuroplastic and synaptic changes in the frontal cortex and/or hippocampus. Full article

Background: Antibiotics at sub-inhibitory concentrations can rewire bacterial regulatory networks, impacting virulence. Objective: The way that exposure to selected antibiotics (ciprofloxacin, amikacin, azithromycin, ceftazidime, and meropenem) below their minimum inhibitory concentration (sub-MIC) modulates the physiology of Pseudomonas aeruginosa is examined in this study using growth-phase-resolved analysis. Methods: Standard P. aeruginosa strain cultures were exposed to ¼ and ½ MIC to determine the growth kinetics under antibiotic stress. The study measured protease and pyocyanin production and the expression level of important quorum sensing and virulence genes (lasI/R, rhlI/R, pqsR/A, and phzA) at different growth phases. Results: Meropenem produced the most noticeable growth suppression at ½ MIC. Sub-MIC antibiotics did not completely stop growth, but caused distinct, dose-dependent changes. Azithromycin eliminated protease activity in all phases and had a biphasic effect on pyocyanin. Ciprofloxacin consistently inhibited both pyocyanin and protease in all phases. The effects of amikacin varied by phase and dose, while β-lactams markedly increased pyocyanin production during the log phase. In contrast to the plateau phase, when expression was often downregulated or unchanged, most quorum-sensing- and virulence-associated genes showed significant upregulation during the death phase under sub-MIC exposure. Conclusions: These findings indicate that sub-MIC antibiotics act as biochemical signal modulators, preserving stress-adapted sub-populations that, in late growth phases, activate quorum sensing and stress tolerance pathways. Full article

The Tuztaşı low-sulfidation epithermal Au–Ag deposit (Biga Peninsula, Türkiye) records a multi-stage hydrothermal history that can be interpreted through the trace and rare-earth-element (REE) chemistry of quartz. High-precision LA-ICP-MS analyses of five representative quartz samples (23 ablation spots; 10 analytically robust) reveal two fluid stages. Early fluids were cold, dilute meteoric waters (δ¹⁸O₍H₂O₎ ≈ −6.8 to +0.7‰), whereas later fluids circulated deeper, interacted with felsic basement rocks, and evolved in composition. Mineralized quartz displays marked enrichment in As (raw mean = 2854 ± 6821 ppm; filtered mean = 70 ± 93 ppm; one spot 16,775 ppm), K (498 ± 179 ppm), and Sb (57.8 ± 113 ppm), coupled with low Ti/Al (<0.005) and elevated Ge/Si (0.14–0.65 µmol mol⁻¹). Chondrite-normalized REE patterns show pronounced but variable LREE enrichment ((La/Yb)_n ≤ 45.3; ΣLREE/ΣHREE up to 10.8) and strongly positive Eu anomalies (δEu ≤ 9.3) with slightly negative Ce anomalies (δCe ≈ 0.29); negligible Ce–Eu covariance (r² ≈ 0.05) indicates discrete redox pulses. These signatures indicate chemically evolved, reducing fluids conducive to Au–Ag deposition. By contrast, barren quartz is characterized by lower pathfinder-element contents, less fractionated REE profiles, higher Ti/Al, and weaker Eu anomalies. A composite exploration toolkit emerges: As > 700 ppm, As/Sb > 25, Ti/Al < 0.005, Ge/Si > 0.15 µmol mol⁻¹, and δEu ≫ 1 reliably identify ore-bearing zones when integrated with δ¹⁸O data and fluid-inclusion microthermometry from earlier studies on the same vein system. This study provides one of the first systematic applications of integrated trace-element and REE analysis of quartz to a Turkish low-sulfidation epithermal system, offering an applicable model for vectoring mineralization in analogous settings worldwide. Full article

Rare earth elements (REEs) are emerging soil contaminants due to increasing fertilizer use, mining activities, and technological applications. However, few studies have assessed their concentrations in soils or associated environmental risks. Here, we evaluate the influence of land cover types (Eucalyptus plantation, forest, and pasture), parent material, and soil physicochemical properties (predictor variables) on REE content in the Brazilian Atlantic Forest and measure pseudo-total REE content using inductively coupled plasma mass spectrometry (ICP-MS). Differences in REE content across land cover types, parent materials, and soil properties were assessed using similarity and variance analyses (ANOSIM, ANOVA, and Kruskal–Wallis) followed by post hoc tests (Tukey HSD and Dunn’s). We used model selection based on the Akaike criterion (ΔAICc < 2) to determine the influence of predictor variables on REE content. Our results showed that parent materials (igneous and metamorphic rocks) were the best predictors, yielding plausible models (Adj R² ≥ 0.3) for Y, δEu, and La_N/Sa_N. In contrast, Ca:Mg alone provided a plausible model (Adj R² = 0.15) for δCe anomalies, while clay content (Adj R² = 0.11) influenced the Sa_N/Yb_N ratio, though soil properties had weaker effects than parent materials. However, we found no evidence that Eucalyptus plantations or pastures under non-intensive management increase REE content in Brazilian Atlantic Forest soils. Full article

Background/Objectives: Obesity is increasingly recognized as a global health concern due to its association with metabolic disorders and gut microbiota dysbiosis. While probiotics offer promise in regulating gut microbiota and improving host metabolism, strain-specific effects remain underexplored, particularly for canine-derived probiotics. This study aimed to isolate and characterize a novel probiotic strain, Ligilactobacillus animalis LA-1, and evaluate its anti-obesity effects and underlying mechanisms using a high-fat diet (HFD)-induced obese mouse model. Methods: LA-1 was isolated from the feces of a healthy dog and assessed for probiotic potential in vitro, including gastrointestinal tolerance, bile salt hydrolase activity, cholesterol-lowering capacity, and fatty acid absorption. Male C57BL/6J mice were fed either a standard chow diet or an HFD for 16 weeks, with HFD mice receiving oral LA-1 supplementation (2 × 10⁹ CFU/day). Multi-omics analyses, including 16S rRNA gene sequencing, short-chain fatty acid (SCFA) quantification, and untargeted liver metabolomics, were employed to investigate the effects of LA-1 on gut microbiota composition, metabolic pathways, and obesity-related phenotypes. Results: LA-1 supplementation significantly alleviated HFD-induced weight gain, hepatic lipid accumulation, and adipose tissue hypertrophy, without affecting food intake. It improved serum lipid profiles, reduced liver injury markers, and partially restored gut microbiota composition, decreasing the Firmicutes/Bacteroidetes ratio and enriching SCFA-producing genera. Total SCFA levels, particularly acetate, propionate, and butyrate, increased following LA-1 treatment. Liver metabolomics revealed that LA-1 modulated pathways involved in lipid and amino acid metabolism, resulting in decreased levels of acetyl-CoA, triglycerides, and bile acids. Conclusions: L. animalis LA-1 exerts anti-obesity effects via gut microbiota modulation, enhanced SCFA production, and hepatic metabolic reprogramming. These findings highlight its potential as a targeted probiotic intervention for obesity and metabolic disorders. Full article

Sediment accumulation in irrigation channels poses a significant challenge to water resource management, impacting hydraulic efficiency and agricultural sustainability. This study introduces an innovative multidisciplinary framework that integrates advanced image analysis (FIJI/ImageJ 1.54p), statistical validation (RStudio), and vector field modeling with a novel Sinusoidal Morphodynamic Bedload Transport Equation (SMBTE) to predict sediment deposition patterns with high precision. Conducted along the Malacatos River in La Tebaida Linear Park, Loja, Ecuador, the research captured a natural sediment transport event under controlled flow conditions, transitioning from pressurized pipe flow to free-surface flow. Observed sediment deposition reduced the hydraulic cross-section by approximately 5 cm, notably altering flow dynamics and water distribution. The final SMBTE model (Model 8) demonstrated exceptional predictive accuracy, achieving RMSE: 0.0108, R²: 0.8689, NSE: 0.8689, MAE: 0.0093, and a correlation coefficient exceeding 0.93. Complementary analyses, including heatmaps, histograms, and vector fields, revealed spatial heterogeneity, local gradients, and oscillatory trends in sediment distribution. These tools identified high-concentration sediment zones and quantified variability, providing actionable insights for optimizing canal design, maintenance schedules, and sediment control strategies. By leveraging open-source software and real-world validation, this methodology offers a scalable, replicable framework applicable to diverse water conveyance systems. The study advances understanding of sediment dynamics under subcritical (Fr ≈ 0.07) and turbulent flow conditions (Re ≈ 41,000), contributing to improved irrigation efficiency, system resilience, and sustainable water management. This research establishes a robust foundation for future advancements in sediment transport modeling and hydrological engineering, addressing critical challenges in agricultural water systems. Full article

Cardiac remodeling in feline hypertrophic cardiomyopathy (HCM) is poorly understood. To investigate underlying molecular mechanisms, we determined microRNA–mRNA interactions, regulatory networks, and upstream regulators using left ventricle (LV) and left atrium (LA) mRNA and microRNA sequencing datasets from cats with HCM and controls. Upstream regulators, molecules, and pathways associated with ischemia, inflammation, fibrosis, and cellular changes were observed in the HCM heart. In both the HCM LV and LA, TNFα, IL1β, and TGFβ were identified as upstream regulators, along with FGF23, THBS4, and FAMB177 as the top increased molecules. Relevant microRNAs included upstream regulator miR-132, enriched miR-124-3p, miR-122b-3p, miR-146-5p (HCM LV and LA), miR-370, miR-1185-5p, miR-12194-3p (HCM LV), miR-153-3p, miR-185-5p, and miR-185-3p (HCM LA). Macrophage pathways were activated, and granulocyte and agranulocyte adhesion and diapedesis were the most connected pathways. The HIF1α signaling pathway in the HCM LV, upstream regulators miR-1-3p and miR-204, and reduced miR-29 and miR-122-5p suggest cardioprotective mechanisms. Observed in the healthy heart and suspected to be involved in cardiac homeostasis were upstream regulators miR-96, inhibited WNT3A and miR-145, as well as miR-140-5p, miR-141-3p, miR-208b-3p, and miR-885-3p. This study provides further insights into the pathogenesis of HCM, and identifies the factors involved in the maintenance of a healthy LV and LA. Full article