Search Results (97,092)

The frescoed Annunziata Oratory chapel in Riofreddo (Italy), a unique testimony to the pontificate of Martin V, sheds light on the trade routes of Ninfa in the first half of the 15th century. Despite having undergone several restorations in the past (the most recent in the 2010s), the Oratory presents serious conservation issues. At first glance, there are no evident signs of biological colonization; rather, the most obvious damage is attributed to detachments and saline efflorescence. Biological colonization at several points was identified using various diagnostic field and laboratory techniques such as ATPase point analysis, field stereoscopy in visible and UV light, culture-based and molecular approaches, Raman spectroscopy, and SEM analysis, biological colonization at several points was identified. The characterization of salt efflorescence was carried out using ion chromatography analysis. The presence of bacteria, fungi and algae, which are also linked to saline efflorescence, was observed. A clear correlation between the biological colonization and salt efflorescence composition was highlighted by our results, as well as the potential sources of microorganisms and salts via the capillary rise of groundwater. This early diagnostic approach regarding the presence of lithobionts and salt efflorescence demonstrates the complex interplay between environmental factors and microbial colonization, which can lead to biodeterioration processes. Full article

The Dual-frequency Precipitation Radar (DPR) aboard the Global Precipitation Measurement (GPM) mission provides valuable three-dimensional precipitation structure data on a global scale and has been widely used in hydrometeorological research. However, due to its spatial resolution limitations and inherent algorithmic assumptions, the accuracy of GPM precipitation estimates can exhibit systematic biases, especially under complex terrain conditions or in the presence of variable precipitation structures, such as light stratiform rain or intense convective storms. In this study, we evaluated the near-surface precipitation rate estimates from the GPM-DPR Level 2A product using over 1440 min of disdrometer observations collected across China from 2021 to 2023. Based on three years of stable stratiform precipitation data from the Jinghe station, we developed a least squares linear correction model for radar reflectivity. Independent validation using national disdrometer data from 2023 demonstrated that the corrected reflectivity significantly improved rainfall estimates under light precipitation conditions, although improvements were limited for convective events or in complex terrain. To further enhance retrieval accuracy, we introduced a regionally adaptive R–Z relationship scheme stratified by precipitation type and terrain category. Applying these localized relationships to the corrected reflectivity yielded more consistent rainfall estimates across diverse conditions, highlighting the importance of incorporating regional microphysical characteristics into satellite retrieval algorithms. The results indicate that the accuracy of GPM precipitation retrievals is more significantly influenced by precipitation type than by terrain complexity. Under stratiform precipitation conditions, the GPM-estimated precipitation data demonstrate the highest reliability. The correction framework proposed in this study is grounded on ground-based observations and integrates regional precipitation types with terrain characteristics. It effectively enhances the applicability of GPM-DPR products across diverse environmental conditions in China and offers a methodological reference for correcting satellite precipitation biases in other regions. Full article

Groundwater is an important factor for the stability of the subway station pit constructed in the offshore area. To reflect the effects of groundwater drawdown on the stability of the station pit, this work uses a surface settlement formula based on Rayleigh distribution to construct a continuous deformation velocity field based on Terzaghi's mechanism, so as to derive a theoretical calculation method for the safety factor of the deep station pit anti-uplift considering the effect of seepage force. Taking the seepage force as an external load acting on the soil skeleton, a simplified calculation method is proposed to describe the variation in shear strength with depth. Substituting the external work rate induced by self-weight, surface surcharge, seepage force, and plastic shear energy into the energy equilibrium equation, an explicit expression of the safety factor of the station pit is obtained. According to the parameter study and engineering application analysis, the validity and applicability of the proposed procedure are discussed. The parameter study indicated that deep excavation pits are significantly affected by construction drawdown and seepage force; the presence of seepage, to some extent, reduces the anti-uplift stability of the station pit. The calculation method in this work helps to compensate for the shortcomings of existing methods and has a higher accuracy in predicting the safety and stability of station pits under seepage situations. Full article

SARS-CoV-2 has an affinity for binding to the human Angiotensin-converting enzyme 2 (ACE2) receptor through cleavage and conformational changes at the S1–S2 boundary and the receptor binding domain of the spike protein, which is also the most variable part of SARS-CoV-2. This study aimed to investigate the expression of Angiotensin-converting enzyme 2 (ACE2), spike protein, and CD68+ markers in placental tissue to demonstrate a possible correlation with the level of systemic oxidative stress biomarkers in patients who were infected with SARS-CoV-2 during pregnancy. A prospective clinical cohort study was designed to investigate the presence of CD68+ macrophages, ACE2, and spike proteins in placental tissue using immunohistochemical methods and to compare these results with oxidative stress from our previous study. Spike and CD68+ macrophages’ immunoreactivity were more pronounced in the placental tissue of patients from the SARS-CoV-2 group. Placental tissue spike protein and CD68+ immunoreactivity correlate with maternal and fetal Thiobarbituric Acid Reactive (TBARS) levels. This study has confirmed that spike protein expression in placental tissue is associated with the newborn’s stay in intensive neonatal care. Therefore, immunoreactivity analysis for the Spike antigen is important in detecting newborns at risk of early neonatal complications. Full article

Deer tick virus (DTV) is a Tick-Borne Orthoflavivirus endemic to the United States, transmitted to humans through bites from the deer tick, Ixodes scapularis, which is also the primary vector of Borrelia burgdorferi s.l., the causative agent of Lyme disease. Human infection with DTV can result in acute febrile illness followed by central nervous system complications, such as encephalitis and meningoencephalitis. Currently, there are mouse models established for investigating the pathogenesis and clinical outcomes of DTV that mimic human infections, but the strains of mice utilized are refractory to infection with B. burgdorferi s.l. Here, we describe the pathogenesis and clinical outcomes of DTV infection in C3H/HeJ mice. Neurological clinical signs, mortality, and weight loss were observed in all DTV-infected mice during the investigation. Infected animals demonstrated consistent viral infection in their organs. Additionally, neuropathology of brain sections indicated the presence of meningoencephalitis throughout the brain. This data, along with the clinical outcomes for the mice, indicates successful infection and showcases the neuroinvasive nature of the virus. This is the first study to identify C3H/HeJ mice as an appropriate model for DTV infection. As C3H/HeJ mice are already an established model for B. burgdorferi s.l. infection, this model could serve as an ideal system for investigating disease progression and pathogenesis of co-infections. Full article

As people spend extended periods of time indoors, stress and negative emotions caused by work have become increasingly difficult to ignore. Observing window views is widely considered an effective method to alleviate stress and promote mental health. However, the specific visual elements within these views that contribute to stress reduction and the differential restorative benefits across varying compositions remain insufficiently understood. This study focuses on four major visual elements commonly seen through windows: sky, buildings, greenery, and roads. Using a horizontal layering approach, nine window views were created based on different proportions of these elements. Participants were exposed to these views, and their responses were evaluated through the positive and negative affect scale (PANAS), as well as electroencephalographic (EEG) data acquisition. The findings indicate that greenery exhibits the most pronounced positive effect on stress mitigation and the enhancement of positive affect, while the presence of roads is more likely to elicit negative emotional responses. Additionally, the visual richness and structural completeness of the window scenes are found to significantly impact restorative outcomes. These findings provide empirical insights for landscape and architectural design aimed at improving psychological well-being. Full article

Group A rotavirus (RVA) is a leading causative agent of diarrhea in both young animals and humans. In China, multiple genotypes are commonly found within the bovine population. In this study, we investigated 1917 fecal samples from calves with diarrhea between 2022 and 2025, with 695 testing positive for RVA, yielding an overall detection rate of 36.25%. The highest positivity rate was observed in Hohhot (38.98%), and annual detection rates ranged from 26.75% in 2022 to 42.22% in 2025. A bovine rotavirus (BRV) strain, designated 0205HG, was successfully isolated from a fecal sample of a newborn calf. Its presence was confirmed through cytopathic effects (CPEs), the indirect immunofluorescence assay (IFA), electron microscopy (EM), and high-throughput sequencing. Genomic characterization identified the strain as having the G6-P[¹]-I2-R2-C2-M2-A3-N2-T6-E2-H3 genotype constellation. The structural proteins VP2 and VP7, along with nonstructural genes NSP1–NSP4, shared high sequence identity with Chinese bovine strains, whereas VP1, VP4, and NSP5 clustered more closely with human rotaviruses, and VP3 was related to feline strains. These findings highlight the genetic diversity and interspecies reassortment of BRVs in China, underlining the importance of continued surveillance and evolutionary analysis. Full article

Extracts, fractions and the pure compound epifriedelanol of the medicinal plant Synadenium glaucescens have antibacterial properties. Herbal products are generally considered less prone to resistance development than conventional antimicrobials, as they contain multiple compounds, which makes bacteria less likely to develop resistance. However, data supporting this notion are lacking. This study evaluated the development of resistance in Staphylococcus aureus subjected to extract, fractions and epifriedelanol of S. glaucescens. It also identified S. aureus fitness genes contributing to intrinsic resistance to extract of S. glaucescens. Fluctuation and gradient concentration assays were used to determine mutation rates and growth adaptation, respectively, which were lower following exposure to growth in crude extract than the pure compound epifriedelanol. By subjecting 1920 single gene mutants from the Nebraska Transposon Mutant Library to growth in the presence of extract of S. glaucescens, 12 genes were identified as important for natural resistance in S. aureus JE2; however, only mutation in the hemB gene decreased the minimum inhibitory concentration by greater than 4-fold (64-fold). In conclusion, purifying active antimicrobial compounds from S. glaucescens and using them as antibacterial substances as an alternative to crude extract increased the risk of resistance development. Further, the gene hemBappears to have a significant role in the natural resistance to the extracts obtained from S. glaucescens in this study. Full article

Background/Objectives: This study investigates the potential of Anisakis sp. as a novel source of α-Gal (Galα1-3Galβ1-4GlcNAc-R) epitopes capable of inducing allergic sensitization in humans. While α-Gal is classically associated with delayed IgE-mediated hypersensitivity following tick bites, emerging evidence suggests that parasitic helminths such as Anisakis sp. may also express α-Gal-containing glycoconjugates, offering an alternative sensitization pathway. Methods: Protein extracts from Anisakis sp. third-stage larvae and mammalian tissues (beef, pork) were analyzed by SDS-PAGE and Western blot using a monoclonal anti-α-Gal antibody (clone M86), and α-Gal epitopes were detected by ELISA. Sera from urticaria patients, stratified by Anisakis sp. sensitization status, were evaluated for anti-α-Gal IgG, IgE, and IgG4 antibodies. Inhibition assays assessed cross-reactivity. Results: Results confirmed the presence of α-Gal epitopes on Anisakis sp. proteins, with prominent bands at ~250 kDa and 65 kDa. Urticaria patients sensitized to Anisakis sp. exhibited significantly elevated anti-α-Gal antibody levels compared to controls. Inhibition ELISA demonstrated substantial reduction in antibody binding with Anisakis sp. extracts, indicating shared antigenic determinants with mammalian α-Gal. Conclusions: These findings establish Anisakis sp. as a source of α-Gal-containing glycoproteins capable of eliciting specific antibody responses in humans, highlighting a potential parasitic route for α-Gal sensitization. Full article

Background/Objectives: Men have historically contributed significantly to mental health nursing, particularly in inpatient settings, where their presence has supported patient recovery and safety. Despite this legacy, men remain under-represented in the nursing workforce, and addressing this imbalance is critical to workforce sustainability. This study offers a novel contribution by exploring the lived experiences, motivations, and professional identities of men in mental health nursing, an area that has received limited empirical attention. The aim of the study is to examine the characteristics, qualities, and attributes of mental health nurses who are male, which contributes to their attraction to and retention within the profession. Methods: A qualitative interpretive inquiry was conducted among nurses who were male and either currently or previously employed in mental health settings. Two focus groups were conducted using semi-structured questions to explore their career pathways, motivations, professional identities, and perceived contributions. Thematic analysis was used to identify key themes and patterns in their narratives. Results: Seven participants, with 10–30 years of experience, participated. They had entered the profession through diverse pathways, expressing strong alignment between personal values and professional roles. Five themes emerged and centred on mental health being the heart of health, personal and professional fulfillment, camaraderie and teamwork, a profound respect for individuals and compassion, and overcoming and enjoying the challenge. Conclusions: Mental health nurses who are male bring unique contributions to the profession, embodying compassion, resilience, and ethical advocacy. Their experiences challenge traditional gender norms and redefine masculinity in health care. Fostering inclusive environments, mentorship, and leadership opportunities is essential to support their growth. These insights inform strategies to strengthen recruitment, retention, and the future of mental health nursing. Full article

Background: Langerhans Cell Histiocytosis (LCH) is a rare histiocytic hematological disorder that frequently involves the lungs. Due to a lack of data about sex-related differences in LCH, the aim of this study is to evaluate sex-related differences in pulmonary function in a cohort of patients with LCH. Methods: We retrospectively analyzed data from 79 adult patients diagnosed with LCH. Demographic, clinical, and spirometric data were collected and compared by sex. Continuous variables were analyzed using the Mann–Whitney test and categorical variables were analyzed with the Chi-square test. Results: Out of 79 patients, 47 (59.5%) were females and 32 (40.5%) were males. Women showed significantly lower diffusing capacity of the lungs for carbon monoxide (DLCO%) and lower diffusing capacity of the lungs for carbon monoxide per unit of alveolar volume (DLCO/VA%) compared to men. Females showed a trend toward lower small airway indices, including maximal expiratory flow at 25 (MEF₂₅%) and forced expiratory flow at 25–75% (FEF_25–75%), though this was not statistically significant, while the residual volume-to-total lung capacity (RV/TLC) ratio was significantly higher in women. Among the functional parameters, DLCO% showed the highest accuracy (AUC 0.70) in the identification of lung involvement after multivariate regression analysis. Conclusions: Our findings suggest that the combination of lower gas exchange efficiency and increased peripheral air trapping secondary to small airway involvement in female patients may reflect the presence of a distinct functional LCH phenotype in women characterized by early small airway involvement and altered ventilation–perfusion dynamics, which may influence the clinical management of these patients. Furthermore, the moderate predictive value of DLCO% for lung involvement at baseline in LCH women suggests that DLCO may contribute to the detection of LCH women with lung involvement, although it should not be considered a definitive diagnostic test without a prospective and independent external validation. Full article

Plastic waste, particularly poly(ethylene terephthalate) (PET), negatively impacts the environment and human health. Biotechnology could become an alternative to managing PET waste if enzymes ensure the recovery of terephthalic acid with efficiencies comparable to those of chemical treatments. Recent research has highlighted the potential of fungal cutinases, such as wild-type ANCUT1 (ANCUT1^wt) from Aspergillus nidulans, in achieving PET depolymerization. Fungal cutinases’ structures differ from those of bacterial cutinases, while their PET depolymerization mechanism has not been well studied. Here, a reliable model of the ANCUT1^wt was obtained using AlphaFold 2.0. Computational chemistry revealed potential cation-binding sites, which had not been described regarding enzymatic activation in fungal cutinases. Moreover, it allowed the prediction of residues with the ability to interact with a PET trimer that were mutation candidates to engineer the substrate binding cleft, seeking enhancements of PET hydrolysis. Enzyme kinetics revealed that both ANCUT1^wt and ANCUT1^N73V/L171Q (DM) were activated by MgCl₂, increasing the dissociation constant of the substrate and maximal reaction rate. We found that in the presence of MgCl₂, DM hydrolyzed different PET samples and released 9.1-fold more products than ANCUT1^wt. Scanning Electron Microscopy revealed a different hydrolysis mode of these enzymes, influenced by the polymer’s crystallinity and structure. Full article

Anticoagulant rodenticides (ARs) are highly effective, but can be of environmental concern due to primary and secondary non-target exposure, with the latter possible being relevant to domestic cats. Therefore, liver residues of ARs and an alternative rodenticide, α-chloralose, were systematically monitored in domestic cats for the first time in the current study. In 2021 and 2022, the carcasses of 99 cats were collected in Slovenia and liver residues were measured by using solid supported liquid–liquid extraction and LC-MS/MS. The results show that 65% of cats carried at least one rodenticide. The second-generation ARs brodifacoum and bromadiolone were most prevalent and found in 53.5 and 25.3% of the samples, respectively. Of first-generation ARs, coumatetralyl was the most prevalent (21.2% of cats). More compounds were detected at high human population density, low farm density and in rural versus intermediate landscapes, but no effect was found for livestock density. Similar trends were found for the presence of brodifacoum, bromadiolone and all rodenticides combined. Farm density was negatively correlated with brodifacoum liver concentration. Individual factors (cat age, sex, outdoor activity) did not matter. The results indicate that a reasonably populated rural landscape, and not the rural or intermediate environment as such, is the main driver of cat exposure to ARs. The risk quotient (RQ) of worst-case acute brodifacoum poisoning was 1506. In summary, a potential environmental problem is globally highlighted for cats that is probably related to secondary exposure to ARs, with a pattern different to that seen in wild predators. Cats are an appropriate sentinel species for assessing rodenticide exposure and endangerment in the environment. Full article

This research contributes to advance the sustainable production of biofuels and provides insights into the energy and exergy assessment of bio-oil, which is essential for developing environmentally friendly energy production solutions. Energy and exergy analyses were performed to evaluate the pyrolysis of beech wood biomass at 500 °C in an Auger reactor. To improve the quality of the obtained bio-oil, its catalytic deoxygenation was performed within an in-line fluidized catalytic bed reactor using a catalyst based on HZSM5 zeolite modified with 5 wt.% Iron (5%FeHZSM-5). A thermodynamic analysis of the catalytic and non-catalytic pyrolysis system was carried out, as well as a comparative study of the calculation methods for the energy and exergy evaluation for bio-oil. The required heat for pyrolysis was found to be 1.2 MJ/kg_biomass in the case of non-catalytic treatment and 3.46 MJ/kg_biomass in the presence of the zeolite-based catalyst. The exergy efficiency in the Auger reactor was 90.3%. Using the catalytic system coupled to the Auger reactor, this efficiency increased to 91.6%, leading to less energy degradation. Calculating the total energy and total exergy of the bio-oil using two different methods showed a difference of 6%. In the first method, only the energy contributions of the model compounds, corresponding to the major compounds of each chemical family of bio-oil, were considered. In contrast, in the second method, all molecules identified in the bio-oil were considered for the calculation. The second method proved to be more suitable for thermodynamic analysis. The novelties of this work concern the thermodynamic analysis of a coupled system of an Auger biomass pyrolysis reactor and a fluidized bed catalytic deoxygenation reactor on the one hand, and the use of all the molecules identified in the oily phase for the evaluation of energy and exergy on the other hand. Full article

Background: Merkel cell polyomavirus (MCPyV) has been established as an etiological agent in Merkel cell carcinoma (MCC), yet its role in other cutaneous neoplasms remains under investigation. The impact of the host’s immunogenetic characteristics on the persistence of Merkel cell polyomavirus (MCPyV) in non-melanoma skin cancer (NMSC) is not yet well understood. Objective: Our aim was to investigate the presence of MCPyV in various skin lesions, particularly NMSC, and its association with cytokine gene polymorphisms related to immune regulation. Methods: We analyzed 274 skin biopsies (lesional, perilesional, and healthy skin) from 84 patients undergoing dermatological evaluation. MCPyV DNA and polymorphisms in IL-6, IL-10, IFN-γ, and TNF-α genes were detected using PCR-based assays. Results: MCPyV was significantly more prevalent in NMSC and non-cancerous lesions than in surgical margins or healthy skin (p = 0.050 and 0.048, respectively). Concordance between lesion and margin samples was low (κ = 0.305), suggesting microenvironment-specific viral persistence. Notably, high-expression IL-10 genotypes (-1082 GG) and low-expression IL-6 genotypes (-174 AA) were significantly associated with MCPyV detection (p = 0.048 and p = 0.015, respectively). Conclusions: MCPyV preferentially localizes to NMSC lesions, particularly in individuals with immunogenetic profiles favoring viral persistence. Since the role of MCPyV in the pathogenesis of NMSC remains uncertain, our results highlight the need for further studies to clarify whether the lesion’s microenvironment supports viral persistence or indicates a more intricate interaction between the virus and the host, which could be significant for the development of skin cancer. Full article