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27 pages, 40964 KB  
Article
Soil Compaction in Montado Mediterranean Ecosystem: Dolomitic Limestone Application, Sheep Grazing Management and Tree Effects
by João Serrano, Shakib Shahidian, Emanuel Carreira, Francisco J. Moral, Luís L. Paniagua, Rui Charneca and Alfredo Pereira
Sustainability 2026, 18(8), 3962; https://doi.org/10.3390/su18083962 - 16 Apr 2026
Viewed by 312
Abstract
Extensive animal production systems based on dryland pastures in Mediterranean regions have low profit margins. Improvements in soil fertility or grazing management and stocking rates are recognized strategies for reversing this situation and to ensure long-term agricultural sustainability. This article aims to assess [...] Read more.
Extensive animal production systems based on dryland pastures in Mediterranean regions have low profit margins. Improvements in soil fertility or grazing management and stocking rates are recognized strategies for reversing this situation and to ensure long-term agricultural sustainability. This article aims to assess whether this strategy of possible intensification of sheep production has a significant impact on soil compaction, which is a manifestation of soil functionality degradation resulting from trampling. An experimental design with four treatments was implemented (with and without dolomitic limestone application; continuous grazing with low stocking rates, CG-LSR, and deferred grazing with high stocking rates, DG-HSR). The study involved cone index (CI, in kPa) measurements (48 sampling areas, 12 in each treatment) on eight dates during two annual pasture/grazing cycles (2023/2024 and 2024/2025). Other soil parameters, the presence of trees and grazing preferences were also monitored and correlated with CI. The main results showed: (i) significantly higher soil compaction under CG-LSR than under DG-HSR; (ii) a negative and significant effect of soil moisture content (SMC) on CI (r = −0.381; p < 0.05); (iii) a significant CI increase in preferential grazing areas, but only in the topsoil layer (0–10 cm) and with a very weak correlation coefficient (r = 0.172; p < 0.05); and (iv) no significant differences in CI under and outside tree canopy areas (UTC and OTC, respectively) for the depth range of 0–30 cm. These results are good indicators of the desired and sustainable intensification of extensive livestock grazing systems. Full article
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26 pages, 639 KB  
Article
Advancing Life Cycle Assessment of Pasture-Based Beef Systems: A High-Resolution Cradle-to-Grave Framework for Global Benchmarking
by Rodolfo Bongiovanni, Leticia Tuninetti, Javier Echazarreta, Ana Muzlera Klappenbach, Javier Lozano, Leonel Alisio and Mariano Avilés
Sustainability 2026, 18(8), 3930; https://doi.org/10.3390/su18083930 - 15 Apr 2026
Viewed by 394
Abstract
Beef production is widely recognized as a significant contributor to global greenhouse gas emissions, making robust and transparent environmental assessments essential for advancing sustainability within supply chains. This study applies a comprehensive cradle-to-grave Life Cycle Assessment (LCA) to evaluate the environmental performance of [...] Read more.
Beef production is widely recognized as a significant contributor to global greenhouse gas emissions, making robust and transparent environmental assessments essential for advancing sustainability within supply chains. This study applies a comprehensive cradle-to-grave Life Cycle Assessment (LCA) to evaluate the environmental performance of beef destined for export, following ISO 14040, ISO 14044 and ISO 14067 standards and the Product Category Rules for meat of mammals. Sixteen impact categories were quantified for 1 kg of vacuum-packed beef using detailed primary data from a pasture-based production system and a representative processing facility. The total climate change impact was 3.27 × 101 kg CO2eq, with enteric methane and feed production jointly responsible for over 70% of overall impacts. Slaughtering and distribution were associated mainly with fossil energy use and ozone depletion, while soil carbon sequestration partially compensated biogenic emissions. The results were consistent with international benchmarks, highlighting the environmental advantages of pasture-based systems, low fertilizer use, and stable land management. Key hotspots were identified in animal growth, feed efficiency, and manure management, with logistics also contributing notably. Overall, the study provides a high-resolution environmental baseline that can support Environmental Product Declarations and guide targeted mitigation strategies across beef supply chains. While the results are derived from a specific pasture-based production system, the study is positioned as a case-study-based application of a high-resolution LCA framework, illustrating how detailed inventories can support environmental benchmarking and hotspot identification without implying statistical representativeness of all beef production systems. Full article
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14 pages, 1235 KB  
Article
Nitrous Oxide Emissions and Ammonia Volatilization from Brachiaria brizantha cv. Piatã Under Different Nitrogen Rates in the Brazilian Savanna
by Lucas Freires Abreu, Bruno José Rodrigues Alves, Fernanda de Kassia Gomes, Fernando Antônio de Souza, Mônica Matoso Campanha, Edilane Aparecida da Silva, Jason E. Rowntree and Ângela Maria Quintão Lana
Agronomy 2026, 16(7), 744; https://doi.org/10.3390/agronomy16070744 - 31 Mar 2026
Viewed by 471
Abstract
Nitrogen (N) fertilization plays a key role in pasture productivity but also contributes to environmental losses, especially under tropical conditions. This study evaluated the effects of four N rates (0, 50, 75, and 100 kg N ha−1) as urea on soil [...] Read more.
Nitrogen (N) fertilization plays a key role in pasture productivity but also contributes to environmental losses, especially under tropical conditions. This study evaluated the effects of four N rates (0, 50, 75, and 100 kg N ha−1) as urea on soil N dynamics, ammonia (NH3) volatilization, nitrous oxide (N2O) emissions, and biomass accumulation in Brachiaria brizantha cv. Piatã, cultivated in a clayey Oxisol in the Brazilian Savanna. The experiment was conducted over two pasture growth cycles during the late summer and early fall. NH3 volatilization increased with the N rate and showed significant differences in the initial samplings of both cycles. N2O emissions were low, strongly influenced by rainfall, and resulted in emission factors ≤ 0.3%. Soil NH4+ and NO3 concentrations did not differ statistically among treatments. Biomass production increased over time on Cycle 2 but plateaued at greater doses, with no significant differences between treatments. The limited biomass response suggests physiological saturation or environmental constraints. Findings indicate that N losses and use efficiency are shaped by rainfall and plant demand. Full article
(This article belongs to the Special Issue Advances in Grassland Productivity and Sustainability—3rd Edition)
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16 pages, 3393 KB  
Article
Boosting Biomass and Leaf Area with Biol: Morphological and Yield Responses of Pennisetum in the Peruvian Highlands
by Darwin Huaman, Waldyr Tarifa, Misael Rodriguez, Sayda Tecsi-Taipe, Yolvi López-Mendoza, Deysi Ruiz-Llontop, José Américo Saucedo-Uriarte, Héctor V. Vásquez, Flor L. Mejía and Ysai Paucar
Agronomy 2026, 16(7), 737; https://doi.org/10.3390/agronomy16070737 - 31 Mar 2026
Viewed by 480
Abstract
The intensive use of chemical fertilizers in agriculture contributes to environmental pollution, which has driven the search for sustainable alternatives such as organic fertilizers. Among these, biofertilizer has garnered interest due to its potential to improve crop growth and yield. The objective of [...] Read more.
The intensive use of chemical fertilizers in agriculture contributes to environmental pollution, which has driven the search for sustainable alternatives such as organic fertilizers. Among these, biofertilizer has garnered interest due to its potential to improve crop growth and yield. The objective of this study was to evaluate the effect of two types of biofertilizer: Bio Chumbinia (standardized) and traditional biofertilizer, as well as a control treatment (water), on the morphology, growth, yield, and leaf area of Maralfalfa (Pennisetum sp.). Morphological and growth variables were measured every 14 days, while yield and leaf area were evaluated in two successive periods corresponding to 42 days of growth. The results indicated that most morphological and growth parameters were significantly influenced by treatment, time, and evaluation (p < 0.05), except for tiller number, blade number, and the blade emergence rate (p > 0.05). Bio Chumbinia showed superior values compared with the control at 6.0 cm for plant height, 0.1 cm/day in the growth rate, 4.1 cm for blade length, and 1.2 mm for blade width; when compared with the traditional biol, the values were similar. The growth rate and leaf emergence peaked on day 14 and subsequently declined. The fresh and dry matter yields were consistently higher on Bio Chumbinia treatment than others (p < 0.05). Although no differences were found for blade weight and leaf area between Bio Chumbinia and the control, the leaf area in Bio Chumbinia was 1400 cm2 more than the control. The second evaluation showed improved productivity, which is consistent with the higher values on the morphological characteristics. No differences were observed in the leaf-to-stem + sheath dry matter ratio. These results demonstrate the potential of Bio Chumbinia to improve the productive performance of Maralfalfa as a foliar fertilizer in sustainable agricultural systems in Peru. Full article
(This article belongs to the Section Grassland and Pasture Science)
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20 pages, 520 KB  
Review
Application of Plant Growth-Promoting Microorganisms (PGPM) to Enhance the Growth of Cool-Season Forage Grasses
by Jakub Dobrzyński, Wojciech Stopa, Anna Paszkiewicz-Jasińska and Barbara Wróbel
Agriculture 2026, 16(5), 629; https://doi.org/10.3390/agriculture16050629 - 9 Mar 2026
Viewed by 636
Abstract
Cool-season (C3) forage grasses are a cornerstone of temperate grassland systems, where improving productivity, nutritive value, and stress resilience is essential for sustainable forage production. In this context, plant growth-promoting microorganisms (PGPMs) have gained increasing attention as potential alternatives or complements to mineral [...] Read more.
Cool-season (C3) forage grasses are a cornerstone of temperate grassland systems, where improving productivity, nutritive value, and stress resilience is essential for sustainable forage production. In this context, plant growth-promoting microorganisms (PGPMs) have gained increasing attention as potential alternatives or complements to mineral and organic fertilization in grassland management. This review synthesizes current knowledge on the role of bacterial and fungal PGPM in enhancing the growth, nutrient use efficiency, and stress tolerance of C3 forage grasses, with particular emphasis on species of the genus Lolium. Available evidence indicates that PGPMs can substantially improve biomass production and plant performance under both optimal and stress conditions through a range of direct and indirect mechanisms. These include phytohormone production, nitrogen fixation, phosphate solubilization, as well as the activation of antioxidant defense systems and stabilization of plant water relations under stress. While Lolium perenne L. and Lolium multiflorum Lam. remain the most extensively studied model species, comparable growth-promoting responses have also been reported for Dactylis glomerata L., Festuca species, and Festulolium hybrids. Increasing attention is being directed toward bacterial and fungal endophytes, which may provide more persistent physiological benefits due to their close association with plant tissues. However, PGPM effects are often strongly species-, genotype-, and environment-dependent, particularly in complex grassland systems. Overall, PGPMs represent a promising tool for sustainable grassland management, although their effective application will require long-term field studies conducted under realistic meadow and pasture conditions. Full article
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10 pages, 659 KB  
Article
Wet-Bulb Temperature as a Superior Predictor of Milk Yield and Reproductive Performance in Holstein Cows in a Continental Climate
by Onur Erzurum and Tamer Kayar
Vet. Sci. 2026, 13(2), 149; https://doi.org/10.3390/vetsci13020149 - 4 Feb 2026
Viewed by 364
Abstract
This study analyzed how climate variability affects lactation yield and reproduction in Holstein cows in a continental climate. It specifically compared Wet-Bulb Temperature (Twb) with the standard Temperature–Humidity Index (THI). We conducted a retrospective study on a dairy farm in Konya, [...] Read more.
This study analyzed how climate variability affects lactation yield and reproduction in Holstein cows in a continental climate. It specifically compared Wet-Bulb Temperature (Twb) with the standard Temperature–Humidity Index (THI). We conducted a retrospective study on a dairy farm in Konya, Türkiye, analyzing a total of 144 complete lactation records from a herd of 90 cows calving between 2022 and 2023. To rule out nutritional factors, a consistent TMR diet without pasture access was maintained in both years. Average Daily Milk Yield (ADMY) was calculated to adjust for lactation length. Climatic data showed a distinct contrast. Ambient temperatures and THI were similar between years (p > 0.05). However, 2022 was characterized by “humid heat” (high Twb), while 2023 presented a “dry heat” profile with significantly lower Twb (p < 0.001). This difference significantly impacted performance. Cows in the 2023 group produced much higher milk yields (50.55 ± 3.01 kg) than the 2022 group (30.74 ± 0.81 kg) (p < 0.001). Unexpectedly, milk yield peaked during the Autumn and Summer seasons of the low-humidity year. In contrast, fertility declined with thermal load. Poor winter fertility suggested a “carry-over” effect of previous heat stress. These findings show that Twb is a better indicator of thermal comfort than THI in continental climates. Furthermore, low humidity can significantly reduce the negative impact of heat on milk production. Full article
(This article belongs to the Special Issue Innovative Strategies to Mitigate Heat Stress in Livestock Production)
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18 pages, 6689 KB  
Article
Floristic Composition of Andean Moorlands and Its Influence on Natural Pasture Productivity: Implications for the Sustainable Management of Alpaca Grazing in Guamote, Ecuador
by Maritza Lucia Vaca-Cárdenas, Julio Mauricio Oleas-Lopez, Santiago Fahureguy Jiménez-Yánez, Freddy Renan Costales Zavala, Pedro Vicente Vaca-Cárdenas, Diego Francisco Cushquicullma-Colcha and Marcelo Eduardo Moscoso-Gómez
Conservation 2026, 6(1), 15; https://doi.org/10.3390/conservation6010015 - 2 Feb 2026
Cited by 1 | Viewed by 876 | Correction
Abstract
Alpacas thrive in Andean ecosystems, efficiently converting natural pasture into products such as fiber and meat, making their breeding a production alternative in Guamote. Intensive grazing and the shift in the spatial distribution of plants due to climate change negatively impact the moorlands. [...] Read more.
Alpacas thrive in Andean ecosystems, efficiently converting natural pasture into products such as fiber and meat, making their breeding a production alternative in Guamote. Intensive grazing and the shift in the spatial distribution of plants due to climate change negatively impact the moorlands. In this context, this study analyzed the influence of floristic composition on the productivity and quality of natural pastures. The methodology included a floristic inventory in a sample of 98 cells in four communities, collecting flora data using the Parker method to measure species composition, density, and cover. In addition, soil fertility and nutritional quality of desirable pastures were assessed through physical and chemical analyses. Principal component and cluster analyses were then applied to correlate the variables. The results showed 26 species, with Poaceae and Asteraceae standing out as dominant and abundant. Tablillas and Pull Quishuar stood out for their productivity and carrying capacity (4.83 t/ha), while Galte Bisñag showed high protein and plant vitality in their pastures. Component 1 stood out for its high production (3.71 t/ha) and carrying capacity in fertile soils; Axis 2 linked Galte Bisñag with high nutritional quality and vegetation cover, while Axis 3 related Asaraty with compacted soils and an intermediate balance. The direct influence between floral species and the productivity of natural pastures leads to the exploration and implementation of measures for sustainable grazing. Full article
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13 pages, 297 KB  
Article
Morphogenesis, Structure, and Chemical Composition of Paiaguás Grass Under Different Nitrogen Doses and Deferment Periods
by Armando Alves de Carvalho, Antonio Leandro Chaves Gurgel, Miguel Arcanjo Moreira Filho, Marcos Jácome de Araújo, Tairon Pannunzio Dias-Silva, Sheila Vilarindo de Sousa, Romilda Rodrigues do Nascimento, Luís Carlos Vinhas Ítavo, Rayanne Amorim Ferreira, Janice Maria dos Santos, Edy Vitoria Fonseca Martins, Auanny Jeniffer de Oliveira Silva and Gelson dos Santos Difante
Plants 2026, 15(3), 341; https://doi.org/10.3390/plants15030341 - 23 Jan 2026
Viewed by 469
Abstract
The study evaluated the effects of nitrogen fertilization on the morphogenetic, structural, productive, and nutritional characteristics of Brachiaria brizantha cv. Paiaguás subjected to two stockpiling periods in a pot experiment. The experiment was conducted using a randomized block design in a 4 × [...] Read more.
The study evaluated the effects of nitrogen fertilization on the morphogenetic, structural, productive, and nutritional characteristics of Brachiaria brizantha cv. Paiaguás subjected to two stockpiling periods in a pot experiment. The experiment was conducted using a randomized block design in a 4 × 2 factorial arrangement, with four nitrogen doses (0, 25, 50, and 75 mg N dm−3, applied as urea) and two stockpiling periods (80 and 120 days). Increasing nitrogen doses promoted linear increases in leaf appearance, elongation, and senescence rates, as well as tiller population density, while reducing phyllochron and leaf lifespan. Forage mass increased linearly with nitrogen, ranging from 96.25 to 113.00 g of dry matter per pot, and leaf blade mass showed a similar response. Root mass exhibited a quadratic response, with a maximum estimated value of 49.33 g pot−1 at 60.18 mg N dm−3, this quadratic equation explained 96% of the variation in the results. No significant interaction was observed between nitrogen doses and stockpiling periods for dry matter, crude protein, mineral matter, or neutral detergent fiber contents. However, nitrogen fertilization increased crude protein content across plant fractions, with leaf crude protein rising from about 70 to over 110 g kg−1 dry matter. Nitrogen fertilization at 75 mg N dm−3 combined with an 80-day stockpiling period improves canopy structure, forage production, and nutritional quality of Paiaguás grass, highlighting the importance of synchronizing nitrogen supply with deferment duration in deferred pasture management. Full article
(This article belongs to the Section Plant Physiology and Metabolism)
11 pages, 1027 KB  
Article
Clustering-Based Characterization of Mixed Herds and the Influence of Pasture Fertilization in High-Andean Livestock Systems
by Jesus Nuñez, Felimon Paxi-Meneses, Wilder Cruz and Richard Estrada
Ruminants 2026, 6(1), 5; https://doi.org/10.3390/ruminants6010005 - 8 Jan 2026
Viewed by 516
Abstract
Livestock production in the high Andes is vital for rural livelihoods and food security but is limited by poor pasture quality, environmental variability, and restricted resources. Pasture improvement, achieved through management practices and particularly through fertilization, may enhance productivity and sustainability in high-Andean [...] Read more.
Livestock production in the high Andes is vital for rural livelihoods and food security but is limited by poor pasture quality, environmental variability, and restricted resources. Pasture improvement, achieved through management practices and particularly through fertilization, may enhance productivity and sustainability in high-Andean livestock systems. This study aimed to characterize mixed herds composed of domestic sheep (Ovis aries), alpacas (Vicugna pacos), llamas (Lama glama), and domestic cattle (Bos taurus) and to evaluate the role of pasture fertilization on herd composition and livestock size. Primary data were collected through structured questionnaires administered to 88 randomly selected livestock producers, complemented by direct field observations of grazing areas, corrals, shelters, and water sources. The survey documented herd structure, grazing management, pasture conservation, fertilization practices, and farm infrastructure. Data from multiple farms were analyzed using a clustering approach to group production units with similar characteristics, and statistical models were applied to assess the effects of fertilization, pasture area, and water sources. Three distinct clusters were identified: one dominated by alpacas, another by sheep, and a third by llamas with the most uniform stocking density. Pasture fertilization was most common in the sheep-dominated cluster and was significantly associated with higher sheep numbers, while no significant effects were detected for alpacas, llamas, or cattle. Farms without fertilization showed slightly higher overall livestock size; however, a strong negative interaction between pasture area and lack of fertilization indicated that expanding grazing land alone could not offset low forage quality. These findings suggest that targeted fertilization, when combined with sustainable grazing practices, may contribute to improved herd performance and long-term resilience in heterogeneous Andean livestock systems. Full article
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19 pages, 6675 KB  
Article
Silicate Agrominerals Mitigate Greenhouse Gas Emissions and Enhance Soil Carbon in Tropical Pasture of the Brazilian Cerrado
by Marcos Vinícius Araujo dos Santos, Alexsandra Duarte de Oliveira, Cícero Célio de Figueiredo, João Paulo Guimarães Soares, Giuliano Marchi, Thayná Xavier Santana, Altair César Moreira de Andrade, Daphne Heloísa de Freitas Muniz, José Ferreira Lustosa Filho, Arminda Moreira de Carvalho, Marcos Aurélio Carolino de Sá and Éder de Souza Martins
Agronomy 2026, 16(2), 138; https://doi.org/10.3390/agronomy16020138 - 6 Jan 2026
Cited by 1 | Viewed by 881
Abstract
The mitigation of greenhouse gas emissions in livestock farming is one of the main challenges for agriculture in the Cerrado biome. Among promising practices, the use of soil remineralizers (REM) stands out as a sustainable and complementary alternative to conventional fertilizers. This study [...] Read more.
The mitigation of greenhouse gas emissions in livestock farming is one of the main challenges for agriculture in the Cerrado biome. Among promising practices, the use of soil remineralizers (REM) stands out as a sustainable and complementary alternative to conventional fertilizers. This study evaluated the effects of applying REM derived from basalt and biotite schist on emissions of N2O, CO2 and CH4, the global warming potential (GWP), as well as on soil carbon and nitrogen in Urochloa brizantha cv. BRS Paiaguás pasture. The experiment was conducted in randomized blocks with five treatments (control, KCl, basalt 8.33 Mg ha−1, basalt 40 Mg ha−1, and biotite schist 151 Mg ha−1). Results indicated that KCl and high-dose basalt (40 Mg ha−1) promoted greater accumulated N2O emissions and higher GWP values. In contrast, biotite schist reduced N2O emissions and showed the lowest GWP (81.67 kg CO2 eq. ha−1), while basalt at a moderate dose (8.33 Mg ha−1) increased soil C and N stocks. It is concluded that soil remineralizers, especially those derived from biotite schist, represent viable alternatives to reduce environmental impacts and promote the sustainability of tropical agricultural systems in Cerrado biome. Full article
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12 pages, 442 KB  
Article
Forage Yield of Megathyrsus maximus Cultivars Managed with Two Fertilization Rates in a Sequential Cropping System
by Matheus Silva Rodrigues, Hemython Luis Bandeira do Nascimento, Darliane de Castro Santos, Bárbara Vieira de Freitas, Victória Caroline Sousa Rosa, Tiago do Prado Paim, Jaqueline Balbina Gomes Ferreira, Stéfany Oliveira de Souza, Victória Santos Souza and Patrick Bezerra Fernandes
Grasses 2026, 5(1), 1; https://doi.org/10.3390/grasses5010001 - 5 Jan 2026
Viewed by 565
Abstract
In the oilseed–cereal–forage system, information on the performance of grasses of the genus Megathyrsus maximus and on the optimal fertilization levels for forage production is still scarce. Therefore, this study aimed to evaluate forage yield (FY) of pastures established in crop succession systems [...] Read more.
In the oilseed–cereal–forage system, information on the performance of grasses of the genus Megathyrsus maximus and on the optimal fertilization levels for forage production is still scarce. Therefore, this study aimed to evaluate forage yield (FY) of pastures established in crop succession systems and subjected to two fertilization regimes during the 2020 and 2021 growing seasons, in the municipality of Rio Verde, Brazil. The experiment evaluated four M. maximus cultivars (Paredão, Zuri, Quênia, and Tamani) under two fertilization rates: maintenance (50, 50, 20, and 3.2 kg ha−1 year−1 of N, K, S, and micronutrients, respectively) and enhanced (150, 150, 40, and 6.4 kg ha−1 year−1, respectively). The Zuri cultivar showed a higher daily forage accumulation rate and greater forage leaf mass compared with the other cultivars. In addition, the Zuri and Paredão cultivars achieved the highest FY, reaching 12.80 and 12.10 Mg ha−1, respectively. The Tamani cultivar exhibited a lower neutral detergent fiber concentration and a higher total digestible nutrient concentration, indicating its potential for systems that prioritize higher forage nutritive value. Zuri and Paredão maximize forage yield in crop succession systems, while enhanced fertilization increases yield by 15%, with adoption dependent on economic feasibility. Full article
(This article belongs to the Special Issue The Role of Forage in Sustainable Agriculture)
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17 pages, 1806 KB  
Article
Interpreting Machine Learning Models with SHAP Values: Application to Crude Protein Prediction in Tamani Grass Pastures
by Gabriela Oliveira de Aquino Monteiro, Gelson dos Santos Difante, Denise Baptaglin Montagner, Valéria Pacheco Batista Euclides, Marina Castro, Jéssica Gomes Rodrigues, Marislayne de Gusmão Pereira, Luís Carlos Vinhas Ítavo, Jecelen Adriane Campos, Anderson Bessa da Costa and Edson Takashi Matsubara
Agronomy 2025, 15(12), 2780; https://doi.org/10.3390/agronomy15122780 - 2 Dec 2025
Cited by 1 | Viewed by 2961
Abstract
Machine learning models such as XGBoost show strong potential for predicting pasture quality metrics like crude protein (CP) content in tamani grass (Panicum maximum). However, their ‘black box’ nature hinders practical adoption. To address this limitation, this study applied SHapley Additive [...] Read more.
Machine learning models such as XGBoost show strong potential for predicting pasture quality metrics like crude protein (CP) content in tamani grass (Panicum maximum). However, their ‘black box’ nature hinders practical adoption. To address this limitation, this study applied SHapley Additive exPlanations (SHAP) to interpret an XGBoost model and uncover how management practices (grazing interval, nitrogen fertilization, and pre- and post-grazing heights) and environmental factors (precipitation, temperature, and solar radiation) jointly influence CP predictions. Data were divided into 80% for training/validation and 20% for testing. Model performance was assessed with stratified 5-fold cross-validation, and hyperparameters were tuned via grid search. The XGBoost model yielded a Pearson correlation coefficient (r) of 0.78, a mean absolute error (MAE) of 1.45, and a coefficient of determination (R2) of 0.57. The results showed that precipitation in the range of 100–180 mm increased the predicted CP content. Application of 240 kg N ha−1 year−1 positively affected predicted CP, whereas a lower dose of 80 kg N ha−1 year−1 had a negative impact, reducing predicted levels of CP. These findings highlight the importance of integrated management strategies that combine grazing height, nitrogen fertilization, and grazing intervals to optimize crude protein levels in tamani grass pastures. Full article
(This article belongs to the Special Issue Precision Farming Applied to Grazing Lands)
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22 pages, 1661 KB  
Article
Effect of Mineral Fertilization on Vegetation of HNV Pastures in the Apuseni Mountains (Romania)
by Ioana Ghețe, Ioan Rotar, Anca Pleșa, Alexandru Ghețe, Claudiu Șerban and Vlad Stoian
Plants 2025, 14(23), 3564; https://doi.org/10.3390/plants14233564 - 21 Nov 2025
Cited by 6 | Viewed by 909
Abstract
High-Nature-Value (HNV) grasslands in the Apuseni Mountains represent traditional semi-natural ecosystems with high biodiversity and major ecological value, but are constantly exposed to pressures generated by both agricultural intensification and abandonment. This study asses the effects of long-term mineral fertilization on floristic composition [...] Read more.
High-Nature-Value (HNV) grasslands in the Apuseni Mountains represent traditional semi-natural ecosystems with high biodiversity and major ecological value, but are constantly exposed to pressures generated by both agricultural intensification and abandonment. This study asses the effects of long-term mineral fertilization on floristic composition and grassland diversity within a permanent experiment initiated in 2001 in Gârda de Sus (Romania). Four variants of mineral fertilization were tested: control (N0), low-input (N50P25K25), medium-input (N100P50K50) and high-input (N150P75K75). Floristic analyses were performed using the modified Braun-Blanquet method, and the data were interpreted using multivariate analyses and diversity indices. The results revealed a clear trophic gradient. Both the control and low-input variants maintained high diversity and the stability of communities dominated by Festuca rubra and its associated oligotrophic species. In contrast, medium and high fertilization produced a pronounced reduction in community components, with the dominance of nitrophilous species, especially Agrostis capillaris, a species which is consumed by animals. Multivariate analyses confirmed that the dominant effect on plant communities was the fertilization level, outweighing the interannual climatic variations. Low fertilization maintained biodiversity with minimal changes after 17 years, while higher inputs led to significant species losses and reduced stability of community. The results provide a scientific benchmark for creating specific sustainable management scenarios and highlight the need for accepted organic alternatives. This study is one of the few long-term experiments in the Carpathians that documents the impact of fertilization on HNV grasslands and provides essential benchmarks for adapting management strategies in the context of the Common Agricultural Policy. Full article
(This article belongs to the Section Plant Ecology)
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19 pages, 3319 KB  
Article
Animal Supplementation and Legume Pastures Enhance Nitrogen Balance and Efficiency in Integrated Crop-Livestock Systems
by Mirella Danna, Fernanda Bernardi Scheeren, João Henrique Silva da Luz, Luis Fernando Glasenapp de Menezes, Wagner Paris, Caroline Amadori, Nathalia Andriotti, Caio Emanuell Garrett, Fernando Ferrari Putti and Laercio Ricardo Sartor
Agriculture 2025, 15(22), 2394; https://doi.org/10.3390/agriculture15222394 - 20 Nov 2025
Cited by 1 | Viewed by 996
Abstract
Improving sustainability in agricultural systems depends on increasing the efficiency of nitrogen (N) use and recycling. This study evaluated whether animal supplementation and legume-based pastures can enhance N balance and residual N availability in an integrated crop-livestock system (ICLS). The experiment was conducted [...] Read more.
Improving sustainability in agricultural systems depends on increasing the efficiency of nitrogen (N) use and recycling. This study evaluated whether animal supplementation and legume-based pastures can enhance N balance and residual N availability in an integrated crop-livestock system (ICLS). The experiment was conducted in two phases—livestock and cropping—using three treatments: a control pasture (oat + ryegrass), a legume mixture (oat + ryegrass + arrowleaf clover), and a supplementation treatment (oat + ryegrass with concentrate supplementation at 1% of live weight), each replicated three times. Soybeans were grown during the cropping phase. Supplementation increased the stocking rate by 21%, while both supplementation and legumes led to a 30% increase in residual N returned via feces and urine, without negatively affecting soybean yield (~4.1 Mg ha−1). N off-take by soybean grain was approximately 9% higher in these treatments, while N exported via cattle carcasses remained unchanged across treatments, averaging 8.2 kg ha−1. Overall, soybeans accounted for 96–97% of total N export, and animals for only 3–4%. These results demonstrate that animal supplementation and legume integration enhance N use efficiency and contribute to nutrient recycling in ICLS, offering a viable strategy to reduce dependence on synthetic fertilizers. The findings support the development of more sustainable livestock and crop systems by maximizing nutrient retention, maintaining yield, and improving soil fertility. Furthermore, the implications for soybean yield and the sustainability of livestock systems indicate a potential positive economic and environmental impact for producers and policymakers. Full article
(This article belongs to the Section Farm Animal Production)
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18 pages, 3542 KB  
Article
Dynamic Changes in Carbon and Nitrogen Storage and Sequestration of Alfalfa Pastureland in Different Planting Years Under Temperate Continental Arid Climate Conditions
by Xin Lu, Juan Qi, Xiangjun Meng, Junhu Su, Ximing Qi and Liyu Shen
Plants 2025, 14(22), 3432; https://doi.org/10.3390/plants14223432 - 10 Nov 2025
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Abstract
Alfalfa (Medicago sativa L.), a drought-tolerant legume, significantly influences carbon and nitrogen cycling in arid and semi-arid regions. This study investigated carbon and nitrogen storage and sequestration dynamics in alfalfa pastureland cultivated for 2–7 years under temperate continental arid climate conditions (110–190 [...] Read more.
Alfalfa (Medicago sativa L.), a drought-tolerant legume, significantly influences carbon and nitrogen cycling in arid and semi-arid regions. This study investigated carbon and nitrogen storage and sequestration dynamics in alfalfa pastureland cultivated for 2–7 years under temperate continental arid climate conditions (110–190 mm annual precipitation). Overall, the biomass, carbon and nitrogen sequestration in alfalfa pasture, and carbon and nitrogen storage and sequestration in soil exhibited a quadratic pattern with planting years. The above-ground biomass peaked at 19.28 t·hm−2, with carbon and nitrogen sequestration reaching the highest level at 10.18 t·hm−2 and 0.511 t·hm−2, respectively, in year 5. Both annual carbon and nitrogen sequestration of the below-ground vegetation exhibited an increase, reaching a peak before decreasing with planting year, and from Y3 to Y7, the sequestration values were consistently higher than those in Y2. Soil carbon and nitrogen sequestration peaked in year 3. Compared to the adjacent fallow lands, alfalfa pasturelands maintained positive soil carbon sequestration until year 6 but became negative (−8.03 t·hm−2) by year 7. From years 2–6, alfalfa pasture fixed carbon and nitrogen at comparable rates but returned disproportionately less carbon than nitrogen to the soil. To optimize sustainability, we recommend (1) rotating alfalfa after 6 years to prevent soil nutrient depletion and (2) applying carbon-rich fertilizers post-year 3 to balance nutrients and prolong productivity in arid climates. Full article
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