Search Results (3,761)

The neurovascular unit (NVU) is an essential, dynamic multicellular unit that maintains the homeostasis and function of the brain, with the integrity of the NVU having a tremendous impact on the pathogenic progression of ischemic stroke (IS). MicroRNAs (miRNAs) are essential regulators of gene expression and promote intercellular communication and functional unity in the NVU. This narrative review assesses the regulatory process mediated by miRNAs that help maintain homeostasis of the NVU, particularly during IS, with particular emphasis on their modulation of tight-junction (TJ) proteins, basement membrane (BM) and glial–vascular. These regulatory actions are essential for blood–brain barrier (BBB) integrity and neuronal survival. The analysis also exposes the intercommunication networks established by key miRNAs between various cells of the NVU, highlighting their complex and dynamic regulatory properties. Moreover, it investigates therapeutic strategies predicated on miRNA regulatory mechanisms, highlighting the optimistic prospects as well as the present limitations pertaining to effective IS intervention. Full article

To overcome the high cost, marine ecological risks of traditional coral sand reinforcement, and the insufficient mechanical performance of standalone Enzyme-Induced Carbonate Precipitation (EICP), this study proposes a novel soil improvement method integrating EICP with aluminum chloride hexahydrate (AlCl₃·6H₂O). The objectives are to identify optimal EICP curing parameters, evaluate AlCl₃·6H₂O’s enhancement effect, and reveal the synergistic micro-mechanism. Through aqueous solution, unconfined compressive strength, permeability, X-ray diffraction (XRD), nuclear magnetic resonance (NMR), and Scanning Electron Microscope (SEM) tests, this study systematically investigated the reaction conditions, mechanical properties, anti-seepage performance, mineral composition, and pore structure. The results demonstrate that EICP achieves the best curing effect under specific conditions: temperature of 30 °C, pH of 8, and cementing solution concentration of 1 mol/L. Under these optimal conditions, the unconfined compressive strength of EICP-solidified coral sand columns reaches 761.6 kPa, and the permeability coefficient is reduced by one order of magnitude compared to unsolidified samples. Notably, AlCl₃·6H₂O incorporation yields a significant synergistic effect, boosting the UCS to 2389.1 kPa (3.14 times standalone EICP) and further reducing permeability by 26%. Micro-mechanism analysis reveals that AlCl₃·6H₂O acts both by generating cementitious aggregates that provide nucleation sites for uniform calcite deposition and by accelerating the transformation of metastable aragonite and vaterite to stable calcite, thereby enhancing cementation stability. This study delivers a cost-effective, eco-friendly solution for coral sand reinforcement, providing practical technical support for marine engineering in environments like the South China Sea. By addressing the core limitations of conventional bio-cementation, it opens new avenues for advancing soil improvement science and applications. Full article

In this paper, the time-dependent properties of the elastic modulus of fly ash concrete under sustained compressive load were studied. An experiment was conducted and showed an increment of elastic modulus for two types of fly ash concrete (20% and 40% fly ash replacement) under sustained load. The mechanisms of this increment were analyzed, and two Representative Volume Elements (RVEs) were established to represent the micro-heterogeneous space of binder and concrete based on continuum mechanics. The shrinking core models of hydration and pozzolanic reaction were adopted to quantify the volume fraction of each phase within the binder RVE. A prediction model was proposed by incorporating the effects of extra hydration and time-dependent aggregate concentration rate under sustained load. Finally, parameter analysis including the influences of initial loading age and the loading level was conducted. Full article

A series of chitosan-based films was obtained by combining the covalent crosslinking of chitosan with dialdehyde starch (DAS) and plasticization using a choline chloride–malonic acid deep eutectic solvent (DES), thereby engineering their structural, mechanical, and surface properties for advanced packaging applications. DAS was synthesized via periodate oxidation of potato starch and characterized by FTIR and quantification of aldehyde groups through acid–base titration, enabling precise control of the –NH₂ (chitosan) to –CHO (DAS) molar ratios (40:1, 20:1, 10:1) used for film formation. Chitosan films (neat, DAS-crosslinked, DES-plasticized, and DES-plasticized/DAS-crosslinked) were obtained by solution casting, with constant total chitosan and/or Ch+DES mass across formulations, and subsequently examined in terms of molecular structure, density, mechanical characteristics, micro- and nanoscale morphology, color, wettability, and surface free energy. The most significant changes relevant to potential applications were observed in mechanical properties and surface free energy. The incorporation of DAS and DES into chitosan resulted in a significant reduction in Young’s modulus from 1150 MPa to 130 MPa, accompanied by a significant increase in elongation at break—from 10% to almost 90%. Moreover, it should be noticed that the addition of DAS and DES led to a nearly twofold increase in surface free energy, from 32.5 to 59.9 mJ m⁻². While previous studies have predominantly focused on single modifications of chitosan—either covalent crosslinking with dialdehyde starch (DAS) or plasticization with deep eutectic solvents (DES)—this work introduces a pioneering dual-modification strategy that simultaneously integrates both techniques, representing the first systematic investigation of their synergistic effects unattainable through individual approaches. Full article

The catalytic hydrogenation of carbon dioxide (CO₂) represents a transformative approach for reducing greenhouse gas emissions while producing sustainable fuels and chemicals, with ethanol being particularly promising due to its compatibility with existing energy infrastructure. Despite significant progress in converting CO₂ to C₁ products (e.g., methane, methanol), selective synthesis of C₂₊ compounds like ethanol remains challenging because of competing reaction pathways and byproduct formation. Recent advances in thermo-catalytic CO₂ hydrogenation have explored diverse catalyst systems including noble metals (Rh, Pd, Au, Ir, Pt) and non-noble metals (Co, Cu, Fe), supported on zeolites, metal oxides, perovskites, silica, metal–organic frameworks, and carbon-based materials. These studies reveal that catalytic performance hinges on the synergistic effects of multimetallic sites, tailored support properties and controlled reaction micro-environments to optimize CO₂ activation, controlled hydrogenation and C−C coupling. Mechanistic insights highlight the critical balance between CO₂ reduction steps and selective C−C bond formation, supported by thermodynamic analysis, advanced characterization techniques and theoretical calculations. However, challenges persist, such as low ethanol yields and undesired byproducts, necessitating innovative catalyst designs and optimized reactor configurations. Future efforts must integrate computational modeling, in situ/operando studies, and renewable hydrogen sources to advance scalable and economically viable processes. This review consolidates key findings, proposes potential reaction mechanisms, and outlines strategies for designing high-efficiency catalysts, ultimately providing reference for industrial application of CO₂-to-ethanol technologies. Full article

Wellbore instability in shale formations represents a worldwide challenge in drilling engineering. The development of high-performance shale stabilizers is crucial for enhancing wellbore stability. A core–shell structured shale stabilizer, designated AAD-CaCO₃, was synthesized via inverse emulsion polymerization using acrylamide (AM), 2-acrylamido-2-methylpropanesulfonic acid (AMPS), and dimethyl diallyl ammonium chloride (DMDAAC) as monomers. Nano-CaCO₃ was generated in situ by reacting calcium chloride and sodium carbonate. Sodium bisulfite and ammonium persulfate were used as initiators. The product was characterized by Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), transmission electron microscopy (TEM), and thermogravimetric analysis (TGA). Its effects on the rheological properties and filtration performance of a bentonite-based mud were evaluated. The stabilizer’s efficacy in inhibiting shale hydration swelling and dispersion was evaluated through linear swelling tests and shale rolling dispersion experiments, while its plugging performance was examined via a filtration loss test with a nanoporous membrane and spontaneous imbibition tests. The results indicated that AAD-CaCO₃ possesses a core–shell structure with the nano-CaCO₃ encapsulated by the polymer. It moderately improved the rheology of the bentonite-based mud and significantly reduced both the low-temperature and low-pressure (LTLP) filtration loss and the high-temperature and high-pressure (HTHP) filtration loss. AAD-CaCO₃ could be adsorbed onto shale surfaces through electrostatic attraction, resulting in substantially reduced clay hydration swelling and an increased shale cutting recovery rate. Effective plugging of micro-nanopores in shale was achieved, demonstrating a dual mechanism of chemical inhibition and physical plugging. Full article

Green innovation efficiency (GIE) serves as a key indicator of urban development toward “dual carbon” goals and sustainable growth. However, systematic evidence remains scarce regarding the impact of the digital-real economy integration (DRI) in urban green innovation efficiency (UGIE). Based on the dual institutional perspectives of government environmental regulation (ER) and intellectual property protection (IPP), this paper proposes an integrated theoretical framework that incorporates integration level, institutional environment, and green innovation. Leveraging panel data from 281 prefecture-level and higher-administered cities in China spanning 2013 to 2023, this paper explores the underlying mechanism and the observed threshold effect of DRI on UGIE. The primary findings are summarized below: (1) DRI promotes UGIE, which is mediated significantly through the institutional roles of ER and IPP. (2) The influence of DRI on GIE is characterized by a threshold effect at a value of 0.9657. Beyond this threshold, the marginal effect rises from 0.47463 to 0.52555, thereby providing evidence for the positive feedback hypothesis between integration level and institutional response. (3) A more significant effect of DRI on GIE could be observed in non-resource-based cities, such as the central cities, southern cities and eastern cities. This paper expands the interdisciplinary research on digital economy and urban sustainability, providing micro-level evidence for the tailored development of digital–green institutional combinations. Full article

The nickel (Ni)-based alloy cladding layers on the surface of 6061 aluminum alloy are fabricated successfully using an optimized laser cladding process. An analysis has been conducted to compare the influence of two types of Ni-based powders on the phase composition, macroscopic morphology and microstructure of the cladding layers. The study also elucidates the micro-hardness and friction property of the cladding layers fabricated by two types of Ni-based powders. The results reveal that phases including Al₃Ni, Al₃Ni₂, and α-Al are formed in the pure Ni cladding layer. Nonetheless, in the Ni–Cr–B–Si cladding layer, a new phase characterized by needle-shaped Cr₇C₃ is observed. Mechanical properties characterization of the cladding layers reveals a notable improvement in microhardness and friction properties compared to the 6061 aluminum alloy substrate. The best properties are achieved in the Ni–Cr–B–Si cladded layer, which demonstrates a microhardness of 714 HV, almost 8.1 times superior to that of the substrate. Its friction and wear rate is merely 21% of that of the base aluminum. Our results are expected to provide significant insights into the design and production of aluminum materials with great resistance to wear. Full article

During the energy storage fracturing process of tight sandstone reservoirs, the pre-injection of fracturing fluid is used to supplement the formation energy, and the physical properties of rocks change under hydration. To reveal the damage mechanism of hydration on tight sandstone, the tight sandstone surrounding the Daqing Changyuan in the northern part of the Songliao Basin was taken as the research object. Through indoor static hydration experiments, combined with scanning electron microscopy (SEM), nuclear magnetic resonance (NMR), Nano-indentation experiments, and other methods, the evolution laws of rock micro-pore morphology, microfracture parameters, Young’s modulus, hardness, and other mechanical indicators under different hydration durations and soaking pressures were systematically explored. The research results show that the water–rock interaction of acidic slick water fracturing fluid significantly changes the mineral composition and microstructure of mudstone and sandstone, controls the development of induced fractures, and degrades the micro-mechanical properties of rocks, with significant lithological differences. In terms of mineral evolution, the soaking time causes the clay minerals in mudstone to increase by up to 12.0%, while pressure causes the carbonate minerals in sandstone to decrease by up to 23.3%. In terms of induced fracture development, the induced fracture widths of sandstone and mudstone under 30 MPa of pressure increase by 122.4% and 85.7%, respectively. The fracture width of mudstone shows a trend of “increasing first and then decreasing” with time, while that of sandstone decreases monotonically. In terms of micro-mechanical properties, after soaking for 168 h, the Young’s modulus of mudstone decreases by up to 66.9%, much higher than that of sandstone (29.5%), while the decrease in hardness of both is similar (58.3% and 59.8%); the mechanical parameters at the induced fractures are only 53.0% to 73.6% of those in the matrix area, confirming the influence of microstructural heterogeneity. This research provides a theoretical basis and data support for optimizing hydraulic fracturing parameters, evaluating wellbore stability, and predicting the long-term development performance in tight sandstone reservoirs. Full article

This work investigates the synthesis, thermodynamic phase stability and microstructural, mechanical and tribological behavior of the CrFeMoV alloy system and its Al-modified derivatives, CrFeMoV-Al2 and CrFeMoV-Al6, which belong to the family of high- and medium-entropy alloys. The studied systems were produced via Vacuum Arc Melting (VAM), followed by a comprehensive characterization. Thermodynamic and geometric phase-formation models were employed to predict the formation of BCC/Β2 solid solutions and the potential emergence of σ-type intermetallic compounds. An ML model was also employed to further predict elemental interactions and phase evolution. These predictions were experimentally confirmed via X-ray diffraction analysis, which verified the presence of a BCC matrix in all compositions, the presence of σ-phase precipitates whose volume fraction systematically reduced with Al inclusion and the gradual increase in the B2 phase with the increase in the Al content. Scanning electron microscopy and EDX analyses uncovered noticeable dendritic segregation, with Mo and Fe enrichment in dendrite cores and in interdendritic regions, respectively. Cr, V, and Al were more uniformly distributed. Mechanical property data derived by micro hardness testing demonstrated a high hardness of 816 HV for the base alloy, ascribed to σ-phase strengthening, followed by a progressive reduction in this value to 802 HV and 756 HV in Al-containing alloys due to the attenuation of σ-phase formation and the gradual increase in the B2 phase. Dry sliding wear results unveiled a positive correlation between wear resistance and hardness, confirming the beneficial role of intermetallic strengthening. Finally, nanoindentation tests shed light on the nanoscale mechanical response, confirming the trends observed at the microscale. Overall, the combination of thermodynamic modeling and experimental analysis provide a robust framework for understanding phase stability, microstructural evolution, and mechanical performance in Al-alloyed CrFeMoV high-entropy systems, while highlighting the potential of controlled Al additions to tailor microstructure and properties. Full article

Silicon nitride (Si₃N₄) ceramic bearings inevitably contain crack-like defects, yet their compressive capacity degradation and crack-driven failure mechanisms remain unclear. This study proposes a discrete element method (DEM) numerical framework within PFC2D to simulate a bearing containing a single prefabricated crack. First, a bearing DEM model was established and calibrated to reproduce the compressive mechanical response. Then, particle deletion introduced controllable central cracks in the ball and raceway with prescribed inclination angles. Finally, displacement-controlled compression-splitting simulations, serving as a surrogate for a quasi-static overload scenario relevant to quality screening, tracked crack initiation, propagation, and failure modes; under a fixed raceway-crack inclination, crack length was varied to quantify size effects. Results show that a single crack markedly reduces compressive strength. Failure progresses through elastic deformation, crack propagation, and final fracture, with cracks initiating at stress concentrators near crack tips. Crack inclination significantly regulates capacity: raceway cracks are most detrimental near 45°, while ball cracks exhibit an overall decrease in initiation and peak stresses with increasing inclination (with local non-monotonicity). Crack length has a stronger weakening effect than inclination, with accelerated capacity loss beyond 0.3 mm and a pronounced drop in initiation stress beyond 0.6 mm. The framework enables controllable defect parametrization and micro-scale failure interpretation for defect sensitivity assessment under compressive overload. Thus, this study focuses on simulating monotonic fracture events to elucidate fundamental defect–property relationships, which provides a foundation distinct from the prediction of rolling contact fatigue life under cyclic service conditions. Full article

This study addresses a key limitation in meso-scale discrete element modeling (DEM) of ultra-high performance concrete (UHPC). Most existing DEM frameworks rely on extensive macroscopic calibration and do not provide a clear, transferable pathway to derive contact law parameters from measurable micro-scale properties, limiting reproducibility and physical interpretability. To bridge this gap, we develop and validate a micro-indentation-informed, poromechanics-consistent calibration framework that links UHPC phase-level micromechanical measurements to a flat-joint DEM contact model for predicting uniaxial compression, direct tension, and flexural response. Elastic moduli and Poisson’s ratios of the constituent phases are obtained from micro-indentation and homogenization relations, while cohesion (c) and friction angle (α) are inferred through a statistical treatment of the indentation modulus and hardness distributions. The tensile strength limit (σₜ) is identified by matching the simulated flexural stress–strain peak and post-peak trends using a parametric set of (c, α, σₜ) combinations. The resulting DEM model reproduces the measured UHPC responses with strong agreement, capturing (i) compressive stress–strain response, (ii) flexural stress–strain response, and (iii) tensile stress–strain response, while also recovering the experimentally observed failure modes and damage localization patterns. These results demonstrate that physically grounded micro-scale measurements can be systematically upscaled to meso-scale DEM parameters, providing a more efficient and interpretable route for simulating UHPC and other porous cementitious composites from indentation-based inputs. Full article

This study investigates the synergistic effects of Cr–Zr and Mn–Zr additions on the microstructural evolution and mechanical properties of Al–6 wt.%Cu alloys. Alloys were designed with solute concentrations positioned below, near, and above their maximum solubility limits, and were evaluated under as-cast, T4, and T6 heat treatment conditions. Mechanical testing revealed distinct behavioral trends depending on the heat treatment: the T4 heat treatment condition generally exhibited superior hardness and yield strength, whereas the T6 heat treatment condition resulted in a slight reduction in hardness but facilitated a significant recovery in tensile strength and structural stability, particularly in alloys designed near the solubility limit. To elucidate the crystallographic origins of these mechanical variations, X-ray diffraction analysis was conducted to monitor changes in lattice parameters, dislocation density, and micro-strain. The results showed that T4 heat treatment induced lattice contraction and a decrease in dislocation density, suggesting that the high strength under T4 heat treatment conditions arises from lattice distortion caused by supersaturated solute atoms. Conversely, T6 aging led to lattice relaxation approaching that of pure aluminum, yet simultaneously triggered a re-accumulation of dislocation density and micro-strain due to the coherency strain fields surrounding precipitates, which effectively impede dislocation motion. Therefore, rather than proposing a single, definitive optimization condition, this study aims to secure foundational data regarding the correlation between these microstructural descriptors and mechanical behavior, providing a guideline for balancing the strengthening contributions in transition metal-modified Al–Cu alloys. Full article

Neuroblastoma is a highly aggressive pediatric malignancy originating from neural crest progenitor cells, predominantly in the adrenal medulla. Amplification of the MYCN oncogene occurs in 20–30% of all neuroblastoma cases and approximately 50% of high-risk tumors, strongly correlating with poor prognosis, relapse, and multidrug resistance. MYCN-driven oncogenesis promotes tumor progression by suppressing apoptotic signaling and enhancing survival pathways, including autophagy—a key mechanism underlying resistance to chemotherapy and immunotherapy. This review examines current therapeutic strategies and resistance mechanisms in MYCN-amplified neuroblastoma, while introducing emerging approaches utilizing exosomes as precision drug delivery systems. Exosomes, nanoscale extracellular vesicles secreted by the tumor cells, exhibit natural tropism and can be engineered to selectively target neuroblastoma-specific biomarkers such as glypican-2 (GPC2), which is highly expressed in MYCN-amplified tumors. Leveraging this property, neuroblastoma-derived exosomes can be purified, modified, and loaded with small interfering RNA (siRNA) to silence MYCN expression, combined with chloroquine—an FDA-approved autophagy inhibitor—to simultaneously inhibit autophagy and induce apoptotic signaling. This dual-targeted approach aims to overcome drug resistance, reduce off-target toxicity, and enhance therapeutic efficacy through exosome-mediated specificity. Furthermore, gut dysbiosis has emerged as a critical factor influencing tumor progression and diminishing treatment efficacy in MYCN-amplified neuroblastoma. We propose integrating microbiota-derived exosomes engineered to deliver anti-inflammatory microRNAs (miRNAs) to the gut mucosa, restoring eubiosis and potentiating systemic anti-tumor responses. Collectively, exosome-based strategies represent a paradigm shift in formulating combination therapies, offering a multifaceted approach to target MYCN amplification, inhibit autophagy, induce apoptosis, and modulate the tumor-microbiome axis. These innovations hold significant promise for improving clinical outcomes in high-risk MYCN-amplified neuroblastoma patients. Full article

Polytetrafluoroethylene (PTFE) is a self-lubricating material but has poor wear resistance. The wear resistance of the composites was enhanced by the incorporation of polyetheretherketone (PEEK), whereas the friction-reducing performance was compromised, thus resulting in an inherent trade-off between wear resistance and lubricity. Graphene nanosheets (GNSs) with high strength and lubricity were introduced as a reinforcement for PEEK/PTFE composites. Composite specimens with varying GNS contents were fabricated and characterized for their mechanical and tribological properties and wear morphologies. Combined with molecular dynamics (MD) simulations, the micro-mechanisms were further elucidated. The optimal GNS content was determined to be 2 wt%, which improved the tensile strength by 10.58% and reduced the wear rate by 17.88% compared to PEEK/PTFE. It achieved the synchronous enhancement of mechanical strength and wear resistance while maintaining desirable friction-reducing performance. MD simulation results demonstrated that the strong interfacial interactions between GNSx and the polymer enabled GNSs to adsorb polymer chains and form a dense rigid network with reduced free volume (FV). The mechanical properties were enhanced by efficient load transfer and the suppression of interfacial delamination enabled by this unique structure; meanwhile, wear resistance was improved due to the mitigation of friction-induced molecular chain scission. Full article