Search Results (71)

Multifunctional scaffolds that combine structural support with the controlled delivery of bioactive agents remain a major challenge in tissue engineering. To extend the use of these devices in biomedicine, 3D printing is presented as an alternative that enables the manufacture of complex devices tailored to each patient, thereby solving specific problems in a timely and efficient manner. In this study, porous 3D scaffolds were fabricated via digital light processing (DLP) using a PET-RAFT resin composed of 2-(dimethylamino)ethyl methacrylate (DMAEMA) and poly(ethylene glycol) diacrylate (PEGDA₅₇₅). Sodium chloride (NaCl) was incorporated as a porogen, while insulin-loaded poly(lactic-co-glycolic acid) (PLGA) nanoparticles were embedded as osteoinductive agents. The printed constructs exhibited high-resolution, reproducible trabecular-like architectures, as confirmed by micro-computed tomography (micro-CT), with interconnected pores averaging 70.7 ± 24.7 μm and a total porosity of 57.0 ± 6.98%. Thermal and chemical analyses confirmed scaffold stability and controlled degradability. Cytocompatibility assays using MC3T3-E1, C2C12, hGMSCs, and C166-GFP cells showed viability above 80% after 7 days (ISO 10993-5). Insulin-loaded nanoparticles enabled sustained release, characterized by an initial burst followed by gradual release up to 72 h. Dynamic bioreactor culture enhanced cell adhesion and RUNX2 expression, confirming the osteoinductive potential of the hybrid scaffold for advanced BTE applications. This study introduces an innovative PET-RAFT-derived resin that combines structural reinforcement with spatiotemporal regulation of insulin release, offering a potential strategy for enhanced biomaterial tissue engineering and tailored therapeutic interventions. Full article

Addressing the lack of high-fidelity test cycles for fuel cell light-duty trucks, this paper proposes an automated driving cycle construction method that integrates unsupervised clustering and reinforcement learning. Firstly, based on large-sample real-world driving data, four libraries of typical driving pattern segments are extracted through dimensionality reduction via Principal Component Analysis (PCA) and K-means clustering. Subsequently, the cycle construction process is formulated as a sequential decision-making problem, and a framework based on the Proximal Policy Optimization (PPO) algorithm, incorporating an action masking mechanism, is designed. This framework innovatively injects macro-level time budget allocation as a hard constraint into the agent’s policy space via action masking, while utilizing micro-level Markov transition probabilities as a soft guide. This dual approach drives the agent to learn an optimal segment concatenation strategy, thereby simultaneously ensuring both the macro-level statistical representativeness and the micro-level driving logic coherence of the synthesized cycle. Validation results demonstrate that the cycle constructed by the proposed method achieves an average relative error of only 7.53% in key characteristic parameters, and its joint speed-acceleration distribution exhibits a similarity as high as 0.9886 with the original data, significantly outperforming traditional methods such as the clustering method, the Markov chain method, and standard driving cycles. This study provides an effective tool for generating high-fidelity driving cycles and testing energy management strategies for fuel cell commercial vehicles. Full article

With the shortage of natural aggregates and the massive accumulation of iron tailings (ITs) solid waste restricting the sustainable development of asphalt pavement engineering, replacing natural aggregates with ITs has become a promising low-carbon solution with prominent economic and social benefits. However, the poor interfacial adhesion between ITs and asphalt severely restricts the engineering application of tailings, and the micro-interaction mechanism at their interface still lacks systematic clarification, which is the key research gap addressed in this work. Different from conventional macro road performance tests, this study innovatively combined molecular dynamics (MD) simulation with microscopic characterization, including Fourier transform infrared spectroscopy (FT-IR) and atomic force microscopy (AFM), to comprehensively reveal the interfacial interaction mechanism between ITs and asphalt at the molecular and microscales. The results indicate that asphalt molecules exhibit higher aggregation concentration and diffusivity on Al₂O₃ and Fe₂O₃ surfaces than on SiO₂ surfaces, proving stronger interfacial interaction between asphalt and iron-rich oxide minerals. Moderate temperature optimizes the adhesion performance of asphalt with Al₂O₃ and Fe₂O₃, while the interfacial bonding of asphalt on CaCO₃ and SiO₂ weakens as temperature rises. The silane coupling agent KH-550 can effectively react with acidic minerals, SiO₂ minerals in ITs, which significantly increases the concentration, diffusion coefficient, and distribution uniformity of asphalt molecules at the interface. FT-IR results verify that the combination of ITs and asphalt mainly relies on physical adsorption without generating new chemical bonds. AFM tests further confirm that alkaline minerals improve the surface roughness of asphalt mastic, and KH-550 greatly enhances the micro-adhesion force of the interface. The novelty of this work lies in clarifying the mechanism of typical mineral components in ITs and revealing the modification enhancement law of silane coupling agent and alkali minerals at the micro level. This study provides a scientific theoretical support for the high-value engineering utilization of ITs in asphalt pavement, and offers a reference for optimizing the interfacial modification design of solid waste aggregate. Full article

Antonio Basoli’s one hundred Picturesque views of the city of Bologna (1824–1836) include 95 preparatory drawings, 16 of which were executed on oil-impregnated lightweight paper now showing advanced degradation. This study aims to investigate the materials and techniques used by Basoli’s workshop and to develop an evidence-based conservation approach for these fragile works. An integrated analytical methodology combining non-invasive hyperspectral imaging (HSI), Fiber Optics Reflectance Spectroscopy (FORS) and Ion Beam Analysis (IBA) with micro-invasive SEM-EDX and FTIR-ATR spectroscopy was applied on five drawings on lightweight impregnated paper to characterize both the paper supports and drawing media. Linseed oil containing lead-based drying agents was suggested to be the impregnating substance, while iron oxide (sanguine) over metalpoint (Cu, Sb, Pb) defined the graphic media. The detection of copper–lead residues suggests that Basoli employed a direct pressure transfer technique similar to James Watt’s copying machine. Conservation treatments resulted in significant pH stabilization (from 5.35 ± 0.20 to 6.45 ± 0.33) and reduced yellowing (ΔE* = 4.9 ± 1.8) while maintaining the paper’s translucency. The results elucidate the innovative practices of Basoli’s workshop and establish a reproducible analytical and conservation methodology applicable to the preservation of nineteenth-century graphic heritage. Full article

MicroRNAs (miRNAs) are key posttranscriptional regulators of gene expression that influence cancer initiation, progression, and therapeutic response. While most studies have focused on endogenous miRNAs, emerging evidence has highlighted the role of plant-derived miRNAs as exogenous dietary regulators capable of cross-kingdom gene modulation. This review summarises current knowledge regarding plant-derived miRNAs and their ability to regulate human cancer-related genes. Experimental findings indicate that plant miRNAs can withstand gastrointestinal digestion, enter the circulation, and regulate the expression of oncogenes, tumour suppressors, long noncoding RNAs, and immune checkpoint molecules via canonical RNA-induced silencing mechanisms. Specific examples include miR-156a, miR-159a-3p, miR-166a, miR-167e-5p, miR-171, miR-395e, miR-2911, miR-4995 and miR-5754, which exhibit anticancer activities across various cancer types and modulate key signalling pathways in mammalian cells, highlighting their potential as cross-kingdom regulators with therapeutic relevance. In addition to these characterised miRNAs, certain plant groups, which are rich in bioactive compounds, remain unexplored as sources of functional miRNAs, representing a promising avenue for future research. Collectively, these studies underscore the ability of plant-derived miRNAs to modulate mammalian gene expression and suggest their potential as diet-based or synthetic therapeutic agents. Further investigations into their bioavailability, target specificity, and functional relevance could inform innovative strategies for cancer prevention, integrating nutritional, molecular biological, and therapeutic approaches. Full article

This review is an exhaustive analysis of the recent progress in the research of active and biologically active additives/ingredients. Fast-developing innovations during the last 3–5 years are included in every stage of their preparation from various resources, with valorization of the by-products and waste, characterization, arising problems with their applications, and an important role in the production of the advanced, high-performance materials. The two main well-known classes as natural (bio)active additives and nanoscaled active additives are discussed as it concerns, their types, classification, characteristic mode of action, green extraction and preparation, green synthesis, supplementary processing performed to achieve the suitable stability of the systems by micro-/nanoemulsification/encapsulation, complexation, etc., importance of the composition–activity relationships, biological effects, antimicrobial, antifungal, antioxidant potential, controlled/targeting release of (bio)active agents, the mechanisms of action of antimicrobials, antifungal and antioxidants synthesized in vivo or derived from the human diet, analytical methods for BACs characterization, degradation, toxicity concerns, a.s.o. The combinations of the two kinds of active additives (including even natural additives prepared at the nanosized level) are frequently used in research and scale-up production of new materials as important innovative solutions and challenges in various application fields, especially when their synergism appears, as will be detailed in the second part of this review. Full article

Permeability evolution in remolded fault gouge creates critical uncertainties in geotechnical parameterization for dam foundations. However, the underlying multi-scale mechanisms, including mineral migration and pore structure changes, remain insufficiently understood. This study investigates these mechanisms using remolded plastic-thrust fault gouge from the Yulong Kashi hydropower project in China. We developed an innovative sample preparation method that combines in situ mineral self-cementation and directional compaction. The study integrated multidisciplinary tests including field in situ permeability tests; seepage–stress coupling tests; and micro-scale NMR/XRD/SEM-EDS analyses. Results demonstrate that remolded samples exhibit 1–2 orders of magnitude lower permeability (10⁻⁷ cm/s) than in situ samples (10⁻⁵ cm/s). This significant reduction is primarily caused by the loss of cementing agents and the uniform compaction of remolded samples, which leads to degraded pore connectivity. SEM-EDS analysis highlighted the leaching of cementing materials (such as K⁺, Ca²⁺ ions), while XRD revealed changes in mineral composition, with chlorite dissolution being the primary mineral alteration associated with permeability decay. Additionally, artificially enhanced cohesion distorted the mechanical behavior of the samples. These findings provide an explanation for why conventional laboratory tests tend to underestimate in situ permeability and overestimate shear strength in fault zones. This study establishes microstructure-informed correction frameworks for hydraulic and mechanical parameters in fault-crossing hydraulic engineering applications Full article

This study focuses on the pre-treatment cleaning technology for the polyimide (PI) substrate of flexible nine-in-one microsensors. The environmentally friendly cleaning agent B, developed by Sea Energe, was innovatively used to replace traditional organic solutions (acetone, methanol, and isopropyl alcohol) to verify its feasibility and application potential in Micro-Electro-Mechanical Systems (MEMS) processes. Cleaning agent B, developed by Sea Energe, was used for the first time to clean the PI substrate of flexible nine-in-one microsensors, and the flexible nine-in-one microsensor was used as a verification platform to compare the cleaning performance with traditional organic solutions (acetone, methanol, and isopropyl alcohol). The experimental results proved that cleaning agent B developed by Sea Energe effectively removed contamination from the PI substrate surface while avoiding the environmental impact and process compatibility issues associated with traditional organic solvents. To verify its reliability, the developed flexible nine-in-one microsensor was embedded in the hydrogen end flow channel of a hydrogen/vanadium redox flow battery (HVRFB) to perform real-time monitoring of multiple parameters, including hydrogen concentration, voltage, current, conductivity, temperature, humidity, flow, pressure, and pH. The experimental results proved that using cleaning agent B, developed by Sea Energe, to clean the PI substrate and the subsequent flexible nine-in-one microsensor resulted in comparable operational stability and measurement accuracy to traditional organic solution (acetone, methanol, and isopropyl alcohol) cleaning processes. This experimental result verifies that cleaning agent B, developed by Sea Energe, not only has an excellent cleaning effect, but also meets the requirements for highly reliable microsensor development, potentially offering an alternative solution for the future introduction of green processes into semiconductors, MEMSs, and various application fields. Full article

In recent years, microneedles (MNs), an innovative transdermal drug delivery system, have demonstrated significant advantages in treating diverse skin diseases. The stratum corneum (SC), with its ‘brick-mortar’ structure, is the main barrier to drug penetration into the skin. MNs—including solid, coated, hollow, dissolving, and hydrogel-forming types—penetrate it minimally to form temporary micro-channels, enabling efficient delivery of a wide range of therapeutic agents. These include small molecules, biologics, nanoparticles, and photosensitizers, among others. This technology has been effectively applied in the treatment of androgenetic alopecia, acne, scars, melanoma, psoriasis, atopic dermatitis, and vitiligo. By avoiding stimulation of dermal blood vessels and nerves, MNs offer low pain and high patient compliance. These advantages underscore their broad clinical potential for dermatologic therapy. Future studies must optimize material selection, drug-carrying efficiency, and scale-up production to facilitate clinical translation. Full article

Vertical alliances within agricultural supply chains serve as critical institutional vehicles for deepening triple-sector integration (primary–secondary–tertiary) in rural economies, driving agricultural modernization, and advancing rural revitalization. However, sustaining alliance stability constitutes a complex dynamic process wherein inadequate stakeholder engagement and collaborative failures frequently precipitate alliance instability or even dissolution. Existing scholarship exhibits limited systematic examination of the micro-mechanisms and regulatory pathways through which multi-agent strategic interactions affect alliance stability from a dynamic evolutionary perspective. To address this gap, this research focuses on China’s core agricultural innovation vehicle—the Agricultural Industrialization Consortium—and examines the tripartite structure of “Leading Enterprise–Family Farm–Integrated Agricultural Service Providers.” We construct a tripartite evolutionary game model to systematically analyze (1) the influence mechanisms governing cooperative strategy selection, and (2) the regulatory effects of key parameters on consortium stability through strategic stability analysis and multi-scenario simulations. Our key findings are as follows: Four strategic equilibrium scenarios emerge under specific conditions, with synergistic parameter optimization constituting the fundamental driver of alliance stability. Specific mechanisms are as follows: (i) compensation mechanisms effectively mobilize leading enterprises under widespread defection, though excessive penalties erode reciprocity principles; (ii) strategic reductions in benefit sharing ratios coupled with moderate factor value-added coefficients are critical for reversing leading enterprises’ defection; (iii) dual adjustment of cost sharing and benefit sharing coefficients is necessary to resolve bilateral defection dilemmas; and (iv) synchronized optimization of compensation, cost sharing, benefit sharing, and value-added parameters represents the sole pathway to achieving stable (1,1,1) full-cooperation equilibrium. Critical barriers include threshold effects in benefit sharing ratios (defection triggers when shared benefits > cooperative benefits) and the inherent trade-off between penalty intensity and alliance resilience. Consequently, policy interventions must balance immediate constraints with long-term cooperative sustainability. This study extends the application of evolutionary game theory in agricultural organization research by revealing the micro-level mechanisms underlying alliance stability and providing a novel analytical framework for addressing the ‘strategy–equilibrium’ paradox in multi-agent cooperation. Our work not only offers new theoretical perspectives and methodological support for understanding the dynamic stability mechanisms of agricultural vertical alliances but also establishes a substantive theoretical foundation for optimizing consortium governance and promoting long-term alliance stability. Full article

As a crucial vehicle for green technological innovation, cooperative networks significantly promote resource integration and knowledge sharing. Yet, their dynamic evolution and micro-mechanism remain underexplored. Drawing on data from the joint applications of green invention patents between 2006 and 2021, this study constructed a multi-agent GTCIN involving multiple stakeholders, such as enterprises, universities, and research institutions, and analyzed the topological structure and evolutionary characteristics of this network; an exponential random graph model (ERGM) was introduced to elucidate its endogenous and exogenous driving mechanisms. The results indicate that while innovation connections increased significantly, the connection density decreased. The network evolved from a “loose homogeneity” to “core aggregation” and then to “outward diffusion”. State-owned enterprises in the power industry and well-known universities are located at the core of the network. Preferential attachment and transitive closure as endogenous mechanisms exert strong and continuous positive effects by reinforcing local clustering and cumulative growth. The effects of exogenous forces exhibit stage-specific characteristics. State ownership and regional location become significant positive drivers only in the mid-to-late stages. The impact of green innovation capability is nonlinear, initially promoting but later exhibiting a significant inhibitory effect. In contrast, green knowledge diversity exerts an opposite pattern, having a negative effect in the early stage due to integration difficulties that turns positive as technical standards mature. Geographical, technological, social, and institutional proximity all have a positive promoting effect on network evolution, with technological proximity being the most influential. However, organizational proximity exerts a significant inhibitory effect in the later stages of GTCIN evolution. This study reveals the shifting influence of endogenous and exogenous mechanisms across different evolutionary phases, providing theoretical and empirical insights into the formation and development of green innovation networks. Full article

The increasing prevalence of antibiotic-resistant bacteria has intensified the need for innovative antibacterial surfaces, particularly in biomedical applications. Traditional approaches often rely on chemical agents alone, which may lead to diminishing efficacy over time. To address this, we investigated the development of a novel antibacterial surface by combining the inherent antimicrobial properties of copper with an engineered surface topography on a biopolymer matrix. A copper–poly-L-lactic acid (Cu-PLLA) composite system was fabricated using sputtering deposition followed by controlled thermal treatment to induce wrinkle-like micro- and nanostructures on the surface. The surface morphology was characterized using scanning electron microscopy (SEM) and atomic force microscopy (AFM), confirming the formation of hierarchical wrinkle patterns. The chemical composition and distribution of copper were analyzed via energy-dispersive X-ray spectroscopy (EDS). Antibacterial performance was assessed against both Gram-negative Escherichia coli and Gram-positive Staphylococcus aureus using standard colony count reduction assays. The Cu-PLLA wrinkled surfaces demonstrated significantly enhanced bactericidal activity compared with flat PLLA and copper-free controls, a finding attributed to a synergistic effect of mechanical membrane disruption and copper-mediated chemical toxicity. These findings suggest that biopolymer–metal hybrid surfaces with engineered topography offer a promising strategy for developing next-generation antibacterial materials suitable for biomedical and clinical use. Full article

Bone integrity is maintained through continuous remodeling, orchestrated by the coordinated actions of osteocytes, osteoblasts, and osteoclasts. Once considered passive bystanders, osteocytes are now recognized as central regulators of this process, mediating biochemical signaling and mechanotransduction. Malfunctioning osteocytes contribute to serious skeletal disorders such as osteoporosis. Mesenchymal stromal cells (MSCs), multipotent stem cells capable of differentiating into osteoblasts, have emerged as promising agents for bone regeneration, primarily through the paracrine effects of their secreted exosomes. MSC-derived exosomes are nanoscale vesicles enriched with proteins, lipids, and nucleic acids that promote intercellular communication, osteoblast proliferation and differentiation, and angiogenesis. Notably, they deliver osteoinductive microRNAs (miRNAs) that influence osteogenic markers and support bone tissue repair. In vivo investigations validate their capacity to enhance bone regeneration, increase bone volume, and improve biomechanical strength. Additionally, MSC-derived exosomes regulate the immune response, creating pro-osteogenic and pro-angiogenic factors, boosting their therapeutic efficacy. Due to their cell-free characteristics, MSC-derived exosomes offer benefits such as diminished immunogenicity and minimal risk of off-target effects. These properties position them as promising and innovative approaches for bone regeneration, integrating immunomodulatory effects with tissue-specific regenerative capabilities. Full article

Antimicrobial food packaging with natural antimicrobials and biodegradable polymers presents an innovative solution to mitigate microbial contamination, prolong freshness, reduce food waste, and alleviate environmental burden. This study developed antimicrobial hemicellulose-based films by incorporating carvacrol (1% and 2%) as a natural antimicrobial agent through micro-emulsification produced by high-pressure homogenization (M-films). For comparison, films with the same formula were constructed using coarse emulsions (C-films) without high-pressure homogenization. These films were investigated for their antimicrobial efficacy, mechanical and barrier properties, and physicochemical attributes to explore their potential as sustainable antimicrobial packaging solutions. The M-films demonstrated superior antimicrobial activity, achieving reductions exceeding 4 Log CFU/mL against Listeria monocytogenes, Escherichia coli, and Salmonella enterica, compared to the C-films. High-pressure homogenization significantly reduced the emulsion’s particle size, from 11.59 to 2.55 μm, and considerably enhanced the M-film’s uniformity, hydrophobicity, and structural quality. Most importantly, the M-films exhibited lower oxygen transmission (35.14 cc/m²/day) and water vapor transmission rates (52.12 g/m²/day) than the C-films at 45.1 and 65.5 cc/m²/day, respectively, indicating superior protection against gas and moisture diffusion. Markedly improved mechanical properties, including foldability, toughness, and bubble-free surfaces, were also observed, making the M-films suitable for practical applications. This study highlights the potential of high-pressure homogenization as a method for enhancing the functional properties of hemicellulose-based films (i.e., M-films). The fabricated films offer a viable alternative to conventional plastic packaging, paving the way for safer and greener solutions tailored to modern industry needs. Full article

In bulk liquid or on solid surfaces, nanobubbles (NBs) are gaseous domains at the nanoscale. They stand out due to their extended (meta)stability and great potential for use in practical settings. However, due to the high energy cost of bubble generation, maintenance issues, membrane bio-fouling, and the small actual population of NBs, significant advancements in nanobubble engineering through traditional mechanical generation approaches have been impeded thus far. With the introduction of the electric field approach to NB creation, which is based on electrostrictive NB generation from an incoming population of “electro-fragmented” meso-to micro bubbles (i.e., with bubble size broken down by the applied electric field), when properly engineered with a convective-flow turbulence profile, there have been noticeable improvements in solid-state operation and energy efficiency, even allowing for solar-powered deployment. Here, these innovative methods were applied to a selection of upstream and downstream activities in the oil–water–fuels nexus: advancing core flood tests, oil–water separation, boosting the performance of produced-water treatment, and improving the thermodynamic cycle efficiency and carbon footprint of internal combustion engines. It was found that the application of electric field NBs results in a superior performance in these disparate operations from a variety of perspectives; for instance, ~20 and 7% drops in surface tension for CO₂- and air-NBs, respectively, a ~45% increase in core-flood yield for CO₂-NBs and 55% for oil–water separation efficiency for air-NBs, a rough doubling of magnesium- and calcium-carbonate formation in produced-water treatment via CO₂-NB addition, and air-NBs boosting diesel combustion efficiency by ~16%. This augurs well for NBs being a potent agent for sustainability in the oil and fuels sector (whether up-, mid-, or downstream), not least in terms of energy efficiency and environmental sustainability. Full article