Search Results (570)

Premature ovarian insufficiency (POI) is an important factor in female infertility and is often associated with oxidative stress. Alginate oligosaccharides (AOSs), derived from the degradation of alginate, have been demonstrated to have protective effects against various oxidative stress-related diseases. However, the impact of AOSs on POI has not been previously explored. The current study explored the effects of AOSs on ovarian dysfunction in a mouse model of POI induced by D-galactose (D-gal). Female C57BL/6 mice were randomly divided into five groups: the control (CON), POI model (D-gal), and low-, medium-, and high-dose AOS groups (AOS-L, 100 mg/kg/day; AOS-M, 150 mg/kg/day; AOS-H, 200 mg/kg/day). For 42 consecutive days, mice in the D-gal, AOS-L, AOS-M, and AOS-H groups received daily intraperitoneal injections of D-gal (200 mg/kg/day), whereas those in the CON group received equivalent volumes of sterile saline. Following D-gal injection, AOSs were administered via gavage at the specified doses; mice in the CON and D-gal groups received sterile saline instead. AOS treatment markedly improved estrous cycle irregularities, normalized serum hormone levels, reduced granulosa cell apoptosis, and increased follicle counts in POI mice. Moreover, AOSs significantly reduced ovarian oxidative stress and senescence in POI mice, as indicated by lower levels of malondialdehyde (MDA), higher activities of catalase (CAT) and superoxide dismutase (SOD), and decreased protein expression of 4-hydroxynonenal (4-HNE), nitrotyrosine (NTY), 8-hydroxydeoxyguanosine (8-OHdG), and p16 in ovarian tissue. Analysis of the gut microbiota through 16S rRNA gene sequencing and short-chain fatty acid (SCFA) analysis revealed significant differences in gut microbiota composition and SCFA levels (acetic acid and total SCFAs) between control and D-gal-induced POI mice. These differences were largely alleviated by AOS treatment. AOSs changed the gut microbiota by increasing the abundance of Ligilactobacillus and decreasing the abundance of Clostridiales, Clostridiaceae, Marinifilaceae, and Clostridium_T. Additionally, AOSs mitigated the decline in acetic acid and total SCFA levels observed in POI mice. Notably, the total SCFA level was significantly correlated with the abundance of Ligilactobacillus, Marinifilaceae, and Clostridium_T. In conclusion, AOS intervention effectively mitigates ovarian oxidative stress, restores gut microbiota homeostasis, and regulates the microbiota–SCFA axis, collectively improving D-gal-induced POI. Therefore, AOSs represent a promising therapeutic strategy for POI management. Full article

Since the onset of industrialization, the safety of arable land has become a pressing global concern, with soil salinization emerging as a critical threat to agricultural productivity and food security. To address this challenge, the cultivation of economically valuable salt-tolerant plants has been proposed as a viable strategy. In the study, we investigated the physiological and molecular responses of Lycium ruthenicum Murr. to varying NaCl concentrations. Results revealed a concentration-dependent dual effect: low NaCl levels significantly promoted seed germination, while high concentrations exerted strong inhibitory effects. To elucidate the mechanisms underlying these divergent responses, a combined analysis of metabolomics and transcriptomics was applied to identify key metabolic pathways and genes. Notably, salt stress enhanced photosynthetic efficiency through coordinated modulation of ribulose 5-phosphate and erythrose-4-phosphate levels, coupled with the upregulation of critical genes encoding RPIA (Ribose 5-phosphate isomerase A) and RuBisCO (Ribulose-1,5-bisphosphate carboxylase/oxygenase). Under low salt stress, L. ruthenicum maintained intact cellular membrane structures and minimized oxidative damage, thereby supporting germination and early growth. In contrast, high salinity severely disrupted PS I (Photosynthesis system I) functionality, blocking energy flow into this pathway while simultaneously inducing membrane lipid peroxidation and triggering pronounced cellular degradation. This ultimately suppressed seed germination rates and impaired root elongation. These findings suggested a mechanistic framework for understanding L. ruthenicum adaptation under salt stress and pointed out a new way for breeding salt-tolerant crops and understanding the mechanism. Full article

Salinization of olive orchards constitutes a front-line agronomic challenge for farmers, consumers, and the scientific community as food security, olive logistics, and land use become more unsustainable and problematic. Plantlets of two olive varieties (var. Kalamon and var. Koroneiki) were tested for their performance under soil saline conditions, in which L-methionine, choline-Cl, and L-proline betaine were applied foliarly to alleviate adverse effects. The ‘Kalamon’ variety ameliorated its photosynthetic rates when L-proline betaine and L-methionine were administered at low saline exposure. The stressed varieties achieved higher leaf transpiration rates in the following treatment order: choline-Cl > L-methionine > L-proline betaine. Choline chloride supported stomatal conductance in stressed var. Kalamon olives without this pattern, which was also followed by var. Koroneiki. Supplementation regimes created a mosaic of responses on varietal water use efficiency under stress. The total phenolic content in leaves increased in both varieties after exogenous application only at the highest levels of saline stress. None of the substances applied to olive trees could stand alone as a tool to mitigate salinity stress in order to be recommended as a solid agronomic practice. The residual exploitation of amino acids by the olive orchard microbiome must also be considered as part of an environmentally friendly, integrated strategy to mitigate salinity stress. Full article

Dunaliella salina, a unicellular and eukaryotic alga, has been found to be one of the most salt-tolerant eukaryotes with a wide range of practical applications. To elucidate the underlying molecular mechanisms of D. salina in response to salinity stress, we performed transcriptome sequencing on samples under different stress conditions. A total of 82,333 unigenes were generated, 4720, 1111 and 2611 differentially expressed genes (DEGs) were identified under high salt stress, oxidative stress and hypertonic stress, respectively. Our analysis revealed that D. salina responds to salinity stress through a complex network of molecular mechanisms. Under high salt stress, starch degradation is regulated by AMY (α-amylase) and PYG (glycogen phosphorylase) with alternative expression patterns. This process is hypothesized to be initially constrained by low ATP levels due to impaired photosynthesis. The clustering analysis of DEGs indicated that starch and sucrose metabolism, as well as glycerol metabolism, are specifically reprogrammed under high salt stress. Glycerol metabolism, particularly involving GPDHs, plays a crucial role in maintaining osmotic balance under salinity stress. Key glycerol metabolism genes were up-regulated under salinity conditions, indicating the importance of this pathway in osmotic regulation. The G3P shuttle, involving mitochondrial GPDHs (c25199_g1 and c23777_g1), contributes to redox imbalance management under high salt, oxidative and hypertonic stresses. Notably, c23777_g1 is involved in the G3P shuttle under high salt, oxidative and hypertonic stresses, while c25199_g1 is specifically induced by hypertonic stress. The R2R3-MYB gene (c23845_g1) may respond to different effects of salinity stress by regulating the transcription of ROS-related genes. Our study provides a detailed understanding of the molecular responses of D. salina to salinity stress. We reveal the critical roles of starch and sucrose metabolism, glycerol metabolism and transcription factors in the D. salina adaptation to salinity. Full article

Soil saline–alkaline stress and water stress, exacerbated by anthropogenic activities and climate change, are major drivers of wetland vegetation degradation, severely affecting the function of wetland ecosystems. In this study, we conducted a simulation experiment with three water levels and four saline–alkaline concentration levels as stress factors to assess eight key functional traits of Phragmites australis and Bolboschoenus planiculmis, dominant species in the salt marsh wetlands in the western region of Jilin province, China. The study aimed to evaluate how these factors influence the functional traits of P. australis and B. planiculmis. Our results showed that the leaf area, root biomass, and clonal biomass of P. australis significantly increased, and the leaf area of B. planiculmis significantly decreased under low and medium saline–alkaline concentration treatments, while the plant height, ramet number, and aboveground biomass of P. australis and the root biomass, clonal biomass, and clonal/belowground biomass ratio of B. planiculmis were significantly reduced and the ratio of belowground to aboveground biomass of B. planiculmis significantly increased under high saline–alkaline concentration treatment. The combination of drought conditions with medium and high saline–alkaline treatments significantly reduced leaf area, ramet number, and clonal biomass in both species. The interaction between flooding water level and medium and high saline–alkaline treatments significantly suppressed the plant height, root biomass, and aboveground biomass of both species, with the number of ramets having the greatest contribution. These findings suggest that the effects of water levels and saline–alkaline stress on the functional traits of P. australis and B. planiculmis are species-specific, and the ramet number–plant height–root biomass (RHR) strategy may serve as an adaptive mechanism for wetland clones to environmental changes. This strategy could be useful for predicting plant productivity in saline–alkaline wetlands. Full article

Biochar is a carbon-rich material produced through the pyrolysis of agricultural waste. It effectively enhances the physical, chemical, and biological properties of salinity-affected soils. Chickpea (Cicer arietinum L.) is the world’s third most important legume crop, currently cultivated in over 50 countries. However, no study has yet established recommended biochar application rates for this crop under saline soil conditions. Therefore, this study aimed to assess the chemical properties of a clay soil following the application of varying rates of biochar and NaCl, and to evaluate their subsequent effects on the growth and yield of Cicer arietinum L. To evaluate the effect of biochar, a completely randomized experimental design with ten replicates was implemented. The biochar was produced from corncobs (Zea mays) and applied at two rates (1.5% and 3%). Soil salinity levels were classified into three groups: non-saline (S1 = 1.2 dS·m⁻¹), low/moderate salinity (S2 = 4.2 dS·m⁻¹), and moderate salinity (S3 = 5.6 dS·m⁻¹). The treatments were placed in pots for 100 days. The results demonstrated that biochar applications at 1.5% and 3% rates improved both soil chemical properties (pH, EC, SAR, and ESP) and the growth of C. arietinum across all evaluated treatments. The 3% biochar treatment showed superior effects compared to the 1.5% application. Therefore, biochar application in C. arietinum production emerges as an effective agronomic strategy to mitigate abiotic stress while simultaneously enhancing crop productivity and sustainability. Full article

Excess-sulfate phosphogypsum slag cement (EPSC), offering the potential for large-scale phosphogypsum (PG) utilization, has drawn significant attention. However, its susceptibility to salt erosion in marine/saline environments remains unquantified, hindering engineering applications. This study, therefore, systematically investigates the effect of various salts (NaCl, MgCl₂, KCl, and Na₂SO₄) at different concentrations (0.5–1.5%) on the hydration mechanism and performance of EPSC using rheometry, strength tests, and microstructural characterization (XRD/SEM-EDS). The findings reveal that EPSC exhibits low initial yield stress and plastic viscosity, both of which increase over time. The addition of Na⁺, Cl⁻, and SO₄²⁻ ions promotes hydration and flocculent structure formation in the EPSC paste, thereby enhancing the yield stress and plastic viscosity. In contrast, Mg²⁺ and K⁺ ions inhibit the hydration reaction, although Mg²⁺ temporarily increases the plastic viscosity by forming Mg(OH)₂ during the initial stage of the reaction. Both Na₂SO₄ and NaCl improve mechanical properties when their concentrations are within the 0.5–1.0% range; however, excessive amounts (>1%) negatively impact these properties. Significantly, adding 0.5% NaCl significantly improves the mechanical properties of EPSC, achieving a 28-day compressive strength of 51.06 MPa—a 9.5% increase compared to the control group. XRD and SEM-EDX analyses reveal that NaCl enhances pore structure via Friedel’s salt formation, while Na₂SO₄ promotes the early nucleation of ettringite. However, excessive ettringite formation in the later stages of the hydration reaction due to Na₂SO₄ may negatively affect compressive strength due to the inherent abundance of SO₄²⁻ in the EPSC system. Therefore, attention should be paid to the effect of excessive SO₄²⁻ on the system. These results establish salt-type/dosage thresholds for EPSC design, enabling its rational use in coastal infrastructure where salt resistance is critical. Full article

Increasing abiotic stress, particularly salinity, poses a significant threat to the germination and seedling development of Tartary buckwheat, thereby limiting its yield potential and broader cultivation. Given Tartary buckwheat’s rich nutritional profile and inherent stress adaptability, enhancing seed tolerance to abiotic stress is essential for ensuring food security and the development of functional food resources. To investigate the role of melatonin in mitigating abiotic stress, seeds of the cultivar ‘Jinqiaomai 2’ were primed with varying melatonin concentrations (with water as the control) at multiple time points. The effects of salt stress on germination and seedling quality were evaluated to determine optimal priming conditions. Subsequent analyses examined seed vigor and physiological and biochemical responses during storage under high temperature and humidity, room temperature, and low-temperature conditions. The results showed that a 3 h melatonin priming consistently resulted in high germination rates (98.7–100.0%). Notably, melatonin at 50 μmol·L⁻¹ was identified as the optimal concentration, significantly improving seedling growth under salinity stress, with increases of 61.1% in seedling length, 59.3% in root length, and 38.9% in root fresh weight compared with the control. Across all storage environments, melatonin-primed seeds exhibited superior vigor and enhanced antioxidant enzyme activity relative to water-primed controls. In conclusion, melatonin priming at an appropriate concentration and duration effectively enhanced the vigor of Tartary buckwheat seeds and alleviated the adverse effects of salinity on germination and storage resilience. However, improved seeds may possess a limited safe storage window and should be sown promptly rather than stored long-term. Full article

Seed germination, a pivotal stage in the plant life cycle, profoundly impacts crop growth and establishment. However, fluctuating environmental conditions like drought, salinity, severe temperatures, and heavy metal toxicity impede seed germination rates and seedling vigor. Seed priming is a pre-sowing seed treatment that involves the controlled hydration of seeds, proven to improve germination rate and stress resilience. It initiates pre-germinative metabolism, including enzyme activity, antioxidant accumulation, hormone modulation, and cellular repair, without radicle emergence. Recent advancements in seed priming, encompassing the application of nanoparticles, phytohormones, and beneficial microbes, have significantly broadened its potential. Despite its proven benefits, challenges such as reduced seed longevity post-priming and variability in species-specific responses remain. This paper revisits the principles and methodologies of seed priming, highlighting its physiological, biochemical, and molecular mechanisms that enhance germination under stress conditions. Additionally, it addresses current challenges and future research directions for optimizing seed priming as a low-cost, eco-friendly approach to improve crop establishment under adverse environments, thereby supporting resilient and sustainable agriculture. Full article

Extreme weather events such as heavy rainfall significantly reduce surface salinity in coastal waters, presenting considerable challenges to the aquaculture of Japanese scallops (Mizuhopecten yessoensis) in shallow cage systems. This study investigated the effects of chronic low-salinity stress on the growth performance, antioxidant capacity, and gene expression profile of M. yessoensis using a 60-day salinity gradient experiment. S33 represents the control treatment with normal seawater salinity (33‰), while S30, S28, and S26 represent experimental groups with progressively lower salinities of 30‰, 28‰, and 26‰, respectively. A decline in salinity was accompanied by an increase in oxygen consumption. The S26 group exhibited a higher ammonia excretion rate (2.73 μg/g·h) than other groups, indicating intensified nitrogen metabolism. Growth was inhibited under low-salinity conditions. The S33 group exhibited greater weight gain (16.7%) and shell growth (8.4%) compared to the S26 group (11.6% and 6%), which also showed a substantially higher mortality rate (46%) compared to the control (13%). At 28‰, antioxidant enzyme activities (T-AOC, SOD, CAT, POD) were elevated, indicating a moderate level of stress. However, at the lowest salinity (26‰), these indicators decreased, reflecting the exhaustion of the antioxidant systems and indicating that the mollusks’ adaptive capacity had been exceeded, leading to a state of stress fatigue. NAD-MDH activity was elevated in the S26 group, reflecting enhanced aerobic metabolism under stress. Transcriptome analysis revealed 564 differentially expressed genes (DEGs) between the S33 and S26 groups. Functional enrichment analysis indicated that these DEGs were mainly associated with immune and stress response pathways, including NF-κB, TNF, apoptosis, and Toll/Imd signaling. These genes are involved in key metabolic processes, such as alanine, aspartate, and glutamate metabolism. Genes such as GADD45, ATF4, TRAF3, and XBP1 were upregulated, contributing to stress repair and antioxidant responses. Conversely, the expressions of CASP3, IKBKA, BIRC2/3, and LBP were downregulated, potentially mitigating apoptosis and inflammatory responses. These findings suggest that M. yessoensis adapts to chronic low-salinity stress through the activation of antioxidant systems, modulation of immune responses, and suppression of excessive apoptosis. This study provides new insights into the molecular mechanisms underlying salinity adaptation in bivalves and offers valuable references for scallop aquaculture and selective breeding programs. Full article

The WRKY transcription factor family is a significant family of plant transcription factors (TFs). Plant growth and development are often influenced by abiotic factors, such as salinity and low temperature. Numerous studies have demonstrated that WRKY TFs primarily influence plant responses to adversity. However, there are few studies on the role of WRKY genes in the stress responses of Malus baccata (L.) Borkh. We cloned the MbWRKY33 gene from Malus baccata for this research, and its roles in salt stress tolerance were analyzed. Phylogenetic tree analysis revealed that MbWRKY33 and PbWRKY33 have the highest homology. Subcellular localization revealed that MbWRKY33 was located within the nucleus. An analysis of tissue-specific expression showed that MbWRKY33 had relatively high expression levels in young leaves and roots. Moreover, Arabidopsis thaliana plants overexpressing MbWRKY33 exhibited stronger resistance to salt stress compared with the wild type (WT) and the unloaded line empty vector (UL). Under the treatment of 200 mM NaCl, transgenic Arabidopsis thaliana plants exhibited significantly higher activities of antioxidant enzymes like superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT) than the control. In contrast, the WT and the UL lines had elevated levels of malondialdehyde (MDA) and reactive oxygen species (ROS). In addition, MbWRKY33 elevates transgenic plant resistance to salt stress by regulating the expression levels of AtNHX1, AtSOS1, AtSOS3, AtNCED3, AtSnRK2, and AtRD29a. Results indicated that MbWRKY33 in Malus might be linked to high-salinity stress responses, laying a foundation for understanding WRKY TFs’ reaction to such stress. Full article

Cultivating crops in the hot, arid conditions of the Arabian Peninsula often requires irrigation with brackish water, which exposes plants to salinity and heat stress. Timely, cost-effective monitoring of plant health can significantly enhance crop management. In this context, remote sensing techniques offer promising alternatives. This study evaluates several low-cost, ground-level remote sensing methods and compares them with benchmark analytical techniques for assessing salt stress in two economically important woody species, moringa and pomegranate. The species were irrigated under three salinity levels: low (2 dS m⁻¹), medium (5 dS m⁻¹), and high (10 dS m⁻¹). Remote sensing tools included RGB, multispectral, and thermal cameras mounted on selfie sticks for canopy imaging, as well as portable leaf pigment and chlorophyll fluorescence meters. Analytical benchmarks included sodium (Na) accumulation, carbon isotope composition (δ¹³C), and nitrogen (N) concentration in leaf dry matter. As salinity increased from low to medium, canopy temperatures, vegetation indices, and δ¹³C values rose. However, increasing salinity from medium to high levels led to a rise in Na accumulation without further significant changes in other remote sensing and analytical parameters. In moringa and across the three salinity levels, the Normalized Difference Red Edge (NDRE) and leaf chlorophyll content on an area basis showed significant correlations with δ¹³C (r = 0.758, p < 0.001; r = 0.423, p < 0.05) and N (r = 0.482, p < 0.01; r = 0.520, p < 0.01). In pomegranate, the Normalized Difference Vegetation Index (NDVI) and chlorophyll were strongly correlated with δ¹³C (r = 0.633, p < 0.01 and r = 0.767, p < 0.001) and N (r = 0.832, p < 0.001 and r = 0.770, p < 0.001). Remote sensing was particularly effective at detecting plant responses between low and medium salinity, with stronger correlations observed in pomegranate. Full article

Morocco’s Témara Plain relies heavily on its aquifer system as a critical resource for drinking water, irrigation, and industrial activities. However, this essential groundwater reserve is increasingly threatened by over-extraction, seawater intrusion, and complex hydrogeochemical processes driven by the region’s geological characteristics and anthropogenic pressures. This study aims to assess groundwater quality and its vulnerability to pollution risks and map the spatial distribution of key hydrochemical processes through an integrated approach combining Geographic Information System (GIS) techniques and multivariate statistical analysis, as well as applying the DRASTIC model to evaluate water vulnerability. A total of fifty-eight groundwater samples were collected across the plain and analyzed for major ions to identify dominant hydrochemical facies. Spatial interpolation using Inverse Distance Weighting (IDW) within GIS revealed distinct patterns of sodium chloride (Na-Cl) facies near the coastal areas with chloride concentrations exceeding the World Health Organization (WHO) drinking water guideline of 250 mg/L—indicative of seawater intrusion. In addition to marine intrusion, agricultural pollution constitutes a major diffuse pressure across the aquifer. Shallow groundwater zones in agricultural areas show heightened vulnerability to salinization and nitrate contamination, with nitrate concentrations reaching up to 152.3 mg/L, far surpassing the WHO limit of 45 mg/L. Furthermore, other anthropogenic pollution sources—such as wastewater discharges from septic tanks in peri-urban zones lacking proper sanitation infrastructure and potential leachate infiltration from informal waste disposal sites—intensify stress on the aquifer. Principal Component Analysis (PCA) identified three key factors influencing groundwater quality: natural mineralization due to carbonate rock dissolution, agricultural inputs, and salinization driven by seawater intrusion. Additionally, The DRASTIC model was used within the GIS environment to create a vulnerability map based on seven key parameters. The map revealed that low-lying coastal areas are most vulnerable to contamination. Full article

The AT-hook motif nuclear localized (AHL) gene family encodes transcription factors pivotal in regulating plant growth, development, and responses to abiotic stimuli, including low temperature, salinity, darkness, and drought. In this study, we systematically identified 21 BpAHL genes in birch and characterized their sequence features, evolutionary relationships, and expression dynamics. Phylogenetic analysis classified BpAHLs into two clades (Clade-A and Clade-B) and three types (Type-I, -II, and -III), based on PPC domain and AT-hook motifs. Chromosomal mapping revealed an even distribution across nine chromosomes and one contig, with dispersed duplication events recognized as the major driver of BpAHL family expansion. Tissue-specific expression profiling uncovered striking divergence: Type-I BpAHLs displayed root-predominant expression, whereas Type-II/III BpAHLs were highly expressed in plant flowers and leaves. Notably, Type-II/III BpAHL genes in leaves showed distinct expression patterns in response to cold and heat stresses, while Type-I BpAHLs in roots were down-regulated under salt stress. This study provides a comprehensive phylogenomic and functional analysis of the AHLs in birch, providing insights into their roles in enhancing abiotic stress resilience in forest trees. Full article

Water quality and the culture performance of juvenile rainbow trout (Oncorhynchus mykiss) were evaluated between 2014 and 2017 in a recirculating aquaculture system (RAS) in the Chilean Altiplano. Key parameters such as temperature, total ammonia nitrogen (TAN), nitrates, and dissolved oxygen were monitored, with values ranging from 7 to 21 °C, <0.1 to 0.63 mg/L, 2.0 to 135 mg/L, and 1.8 to 7.5 mg/L, respectively. Additional parameters—including alkalinity, arsenic, chlorine, true color, conductivity, hardness, phosphorus, pH, potassium, suspended solids, and salinity—were also assessed, comparing different points within the system (head tank, culture tanks, and settling tanks). The results showed that water quality remained within acceptable ranges for aquaculture, although fluctuations in pH and low alkalinity levels caused stress in the fish. Despite these challenges, the specific growth rate (SGR) was 1.49, the feed conversion ratio (FCR) was 1.52, and weight gain reached 298.7%, with a survival rate of 96.2%. This study demonstrates that aquaculture in the Altiplano is feasible and can contribute to the sustainable development of aquaculture in the region. Furthermore, it highlights the importance of comprehensive water quality monitoring to optimize RAS performance in challenging environments. Full article