Search Results (2,512)

Intelligent lower-limb exoskeleton rehabilitation robots are increasingly superseding traditional rehabilitation equipment, making them a focus of research in this field. However, existing systems remain challenged by dynamic instability resulting from various disturbances during actual walking. To address this limitation, this study proposes a novel dynamic stability criterion. Through an analysis of the principles and limitations of the traditional zero-moment point (ZMP) stability criterion, particularly during the late single-leg support phase, a new stability criterion is introduced, which is founded on the swing projection polygon during single-leg support. This approach elucidates the variation patterns of the stability polygon during a single-step motion and facilitates a qualitative analysis of the stability characteristics of the human–robot system in multiple postures. To further enhance the stability and smoothness of gait trajectories in lower-limb exoskeleton rehabilitation robots, the shortcomings of conventional gait planning approaches, namely their non-intuitive nature and discontinuity, are addressed. A recurrent gait planning method leveraging Long Short-Term Memory (LSTM) neural networks is proposed. The integration of the periodic motion characteristics of human gait serves to validate the feasibility and correctness of the proposed method. Finally, based on the recurrent gait planning method, the dynamic stability of walking postures is verified through theoretical analysis and experimental comparisons, accompanied by an in-depth analysis of key factors influencing dynamic stability. Full article

Carbon dioxide capture and storage (CCS) is a technology that can be used to reduce carbon dioxide (CO₂) emissions generated by both natural and anthropogenic industrial processes, particularly petroleum production. To mimic and investigate the effects of CO₂ leakage that may result from CCS, the acute toxicity of seawater acidification induced by continuous CO₂ injection was studied in Asian seabass (Lates calcarifer) fry under static bioassay conditions. Fry (0.828 ± 0.22 g) were exposed to seawater with different pH levels (5.5, 6.0, 6.5, 7.5, and 8.3). Rapid and 100% mortality within 15 min was observed in the pH 5.5 exposure group, while mortality rates ranging from 10.00–41.67% were recorded at 6–96 h in the pH 6.0 exposure group; no mortality was noted in the other pH exposure groups. According to these mortality data, the median lethal concentration at 96 h (96 h LC₅₀) was determined to be a pH of 5.884. Interestingly, after exposure to seawater with pH levels of 5.5 and 6.0, histopathological alterations in the skin, gills, trunk kidney and liver were evident. Additionally, some water quality parameters, especially dissolved oxygen (DO) levels, alkalinity, ammonia levels, and nitrite levels, vary depending on the pH. To further investigate the effects of seawater with pH levels of 8.3 and 5.884 (96 h LC₅₀) and 6.5 (10% safety level) on health status, immune responses and disease susceptibility, fingerling fish (21.25 ± 3.89 g) were studied. Unexpectedly, fish exposed to seawater with a pH of 5.884 rapidly lost muscle control and gradually died, reaching 100% mortality within 24 h, and all response analyses were aborted. Interestingly, with the exception of hematocrit and some immune parameters, various serum innate immune indices, blood biochemistry parameters and immune-related gene expression patterns were similar in fish exposed to seawater with pH levels of 8.3 and 6.5. Additionally, fish were challenged with 0 (control), 1 × 10⁷ and 1 × 10⁹ CFU/mL Vibrio vulnificus, and fish in seawater with a pH level of 6.5 showed a higher sensitivity to 1 × 10⁹ CFU/mL Vibrio vulnificus than fish in seawater with a pH level of 8.3, with mortality rates of 71.24% and 25.44%, respectively (p < 0.05). These findings enhance the understanding of the toxicity effects of seawater acidification caused by CO₂, which will be useful for further assessing the site-specific effects of CCS projects. Full article

Optimizing the growing conditions of Anemone coronaria and Ranunculus asiaticus for cut-flower production under northern greenhouse conditions requires a better understanding of the environmental and cultivation practices influencing emergence, flowering, and flower quality. This study evaluated the effect of storage organ reuse, along with vernalization conditions, growth temperature, growing season, and planting method (in-ground vs. containers) on plant phenology and flower yield and quality. Flower quantity and quality were unaffected by storage organ age, confirming that these organs can be stored and reused the following season. Vernalization at temperatures of 7 °C or 10 °C advanced flowering compared to warmer vernalization in all cultivars, and increased flower yield compared to non-vernalization. Growth under cool conditions (15/10 °C day/night) extended the production period and improved floral quality by promoting longer stems and delaying senescence. Short to moderate photoperiods (11–13 h in the winter vs. 15 h in the spring) and low light intensity, typical of winter, promoted stem elongation and marketable flower yield, whereas increasing photoperiod and temperature in late spring shorten the flowering period. Ground beds provided cooler and more buffered soil conditions, improving flowering duration and yield compared to container-grown plants during springtime. These findings highlight the importance of integrating temperature management, vernalization, and tailored cultivation practices to enhance flower quality, prolong the production, and improve sustainability of cut-flower production under northern climates in both species. Full article

Spring frost poses a major threat to grape-producing regions, severely reducing grape yield and quality. Grafting rootstocks is an effective strategy for enhancing scion resistance to spring frost and mitigating damage. In this study, the two wine grape cultivars (‘Cabernet Sauvignon’ and ‘Chardonnay’) grafted onto three rootstocks (‘Beta’, ‘Kober 5BB’, and ‘3309 Couderc’) were evaluated for their spring frost resistance on one-year-old vines. The scion–rootstock combinations exhibited significantly less photosynthetic impairment under frost stress compared with own-rooted vines. Rootstock also showed lower levels of proline accumulation in the roots and APX activities in the leaves under frost conditions. Compared with own-rooted vines, VvCBF1 gene expression were significantly upregulated in the grafted combinations under frost stress conditions. Among the tested rootstocks, ‘Kober 5BB’ markedly improved the spring frost resistance of both cultivars. CH/5BB exhibited the highest activities of POD and APX activity and the greatest induction of VvCBF genes, along with the lowest relative electrical conductivity and H₂O₂ content. These results highlight the critical role of rootstocks in improving scion spring frost resistance and provide important guidance for selecting suitable rootstocks to mitigate the impact of late frosts. Full article

Methyl-donor nutrients, including folate, vitamin B12, vitamin B6, choline, betaine, and methionine, play indispensable roles in one-carbon metabolism and govern key processes such as DNA methylation, nucleotide synthesis, and genomic maintenance. Yet despite decades of research, their relationship with cancer remains paradoxical and frequently misunderstood. Much of the confusion arises from an overreliance on epidemiological studies that use cancer incidence as a late-stage endpoint, thereby obscuring how the biological actions of methyl donors differ fundamentally across the continuum from precancerous lesions to established tumors. By synthesizing evidence from mechanistic studies, precancerous lesion research, and early-stage carcinogenic models, this review suggests that adequate methyl-donor availability may be protective during the earliest phases of cancer development. However, these same nutrients may later become substrates hijacked by neoplastic cells to fuel rapid proliferation, maintain oncogenic methylation programs, and enhance tumor progression in established malignancies and high-risk populations. Therefore, this review proposes a reframing that methyl donors may not be evaluated merely as protective or harmful, but rather as context-dependent modifiers whose influence is shaped by timing, metabolic status, and the underlying biology of the target tissue. Such a shift is promising for advancing precision nutrition and the prevention or targeted suppression of cancer. Full article

Controlled-release technologies based on natural clays offer a sustainable approach to enhance the efficacy and environmental compatibility of agrochemicals. This study reports the development and evaluation of clay-based azoxystrobin (Az) formulations for controlling Magnaporthiopsis maydis, the causal agent of maize late wilt disease. Among six carriers tested, raw bentonite and sepiolite were selected for their comparable adsorption capacity (9.5% Az loading efficiency) and ease of preparation. A novel mycelial plug-immersion bioassay was established and calibrated (R² = 0.92–0.95) to assess release kinetics and antifungal efficacy, showing approximately tenfold higher sensitivity than conventional disk-diffusion or mycelial-growth inhibition assays. Sequential wash and extended incubation experiments demonstrated sustained Az release equivalent to ≥1 mg L⁻¹ over 144 h, resulting in approximately 50% (p < 0.05) fungal growth suppression. A comparative analysis of particle suspensions and supernatants revealed formulation-specific release behaviors, which differed among clay carriers. Overall, bentonite and sepiolite acted as efficient carriers that prolonged fungicide bioavailability, minimized leaching losses, and preserved biological activity. These findings provide proof of concept for clay–Az formulations as eco-friendly and cost-effective tools for late wilt management and advance understanding of clay–fungicide interactions that support sustainable, integrated disease-control strategies. Full article

Efferocytosis, the process by which macrophages clear apoptotic cells, plays a vital role in maintaining immune homeostasis. This study explores the influence of inflammatory cytokines—tumor necrosis factor-alpha (TNF-α) and transforming growth factor-beta (TGF-β)—on efferocytosis dysregulation in coronary artery disease (CAD). Peripheral blood samples were collected from 27 non-obstructive and 29 obstructive CAD patients to isolate monocytes, which were then differentiated into M1 and M2 macrophages using specific cytokine stimuli. These macrophages were transfected with TNF-α and TGF-β siRNA to assess cytokine impact on efferocytosis. Expression levels of the efferocytosis receptor MERTK and its regulatory protease ADAM17 were quantified via qPCR. Statistical analysis revealed significantly higher MERTK expression in M2 macrophages compared to M1 (p = 0.002). Notably, TNF-α silencing enhanced efferocytosis in M2 macrophages, with increased clearance of early apoptotic bodies in non-obstructive CAD and late apoptotic bodies in obstructive CAD (both p < 0.001). These findings suggest that macrophage phenotype, apoptotic stage, and cytokine environment influence efferocytosis efficiency and may involve pathways beyond MERTK-ADAM17. They offer preliminary mechanistic insights into cytokine-mediated modulation of efferocytosis in CAD. Further in vivo studies are needed to confirm these observations and evaluate their relevance for future therapeutic strategies. Full article

Promoting demand-responsive transit (DRT) is crucial for developing sustainable and green transportation systems in urban areas, especially in light of decreasing transit ridership and increasingly varying demand. However, the effectiveness of such services hinges on their ability to efficiently match varying travel demand. This paper presents a data-driven framework for the joint optimization of customized bus routes and timetables, to enhance both service quality and operational sustainability. Our approach leverages large-scale taxi trip data to identify latent travel demand, applying a spatial–temporal clustering method to group trip requests and identify DRT stops by trip origin, destination, and direction. An adaptive large neighborhood search (ALNS) algorithm is improved to co-optimize passenger waiting times and bus operation costs, where an unbalanced penalty for early or late schedule deviations is developed to better reflect passengers’ discomfort. The framework’s performance is validated through a real-world case study, demonstrating its ability to generate efficient routes and schedules. The model manages to improve passenger experience and reduce operation costs. By creating a more appealing and efficient service, this model contributes directly to the goals of green transport in terms of reducing the total vehicle kilometers that are traveled, and demonstrating a viable, high-quality alternative to private car usage. This study offers a practical and robust tool for transit planners to design a next-generation DRT system that is both economically viable and environmentally sustainable. Full article

Intravesical Bacillus Calmette–Guérin (iBCG) immunotherapy is the standard adjuvant treatment of non-muscle-invasive bladder cancer (NMIBC). Among the potential complications, cases of mycotic aneurysms and acute respiratory distress syndrome (ARDS) are rare but can be life-threatening. Because prior reports have not included whole-genome sequencing (WGS) of clinical Mycobacterium bovis BCG (M. bovis BCG) isolates to assess whether the infecting strain acquires mutations in vivo, we performed WGS in a severe disseminated iBCG-related infection. A 72-year-old man with bladder cancer underwent iBCG instillation. Twelve months after the final instillation, the patient developed an abdominal aortic aneurysm, which was detected and treated with endovascular aneurysm repair (EVAR). Two months later, the patient presented with fever, abdominal pain, and septic shock. Contrast-enhanced computed tomography (CT) and ¹⁸F-fluorodeoxyglucose positron emission tomography/CT (FDG-PET/CT) showed rapid aneurysm enlargement. Ziehl–Neelsen staining and PCR of aortic material identified M. bovis BCG. Direct PCR on BAL fluid and urine was negative; however, BAL and urine culture subsequently grew M. bovis BCG, and PCR performed on the culture isolate confirmed M. bovis BCG. Despite combined antituberculosis triplet therapy (isoniazid, rifampicin, and ethambutol), the patient developed ARDS, which gradually improved after surgical management. WGS (with >96% genome coverage) showed the isolate was highly concordant with the vaccine strain and lacked additional virulence-associated mutations, including in esxM. This case illustrates that severe systemic iBCG-related complications can occur without detectable in vivo acquisition of virulence-enhancing mutations; however, interpretation is limited by the single-case design and the absence of host genetic susceptibility testing. Our findings underscore the need for prolonged vigilance regarding late-onset vascular and pulmonary complications after iBCG, and highlight the importance of early multidisciplinary management. Full article

In the context of global warming, the continued increase in the frequency of compound events—where drought and high-temperature extremes coincide—has led to severe natural disasters and substantial socio-economic losses. To systematically reveal the evolution of summer dry-heat compound events in Liaoning Province, this study constructs a whole-chain analysis framework of “identification–feature extraction–multivariate probability assessment”. Based on the Standardised Precipitation Index (SPI) and the Standardised Temperature Index (STI), we develop the Standardised Dry-Heat Index (SDHI) to identify dry-heat compound events. Run theory is applied simultaneously to extract key attributes for three types of events—drought, high temperature, and dry-heat compound events—and the Mann–Kendall test is used to detect their temporal mutation characteristics. By combining Copula functions with spatial analysis techniques, we further establish a whole-chain analysis method from “identification–feature extraction–hazard quantification”. The results show that during 1961–2020, summer drought, high-temperature, and dry-heat compound events occurred 4, 14, and 10 times, respectively, in Liaoning Province, with all three types showing a significant increase in frequency after the late 1990s. Spatially, zones of high drought intensity are mainly located in western Liaoning; the duration and severity of high temperatures are most pronounced in inland basin areas; and regions with high compound hazard intensity of dry-heat events largely coincide with urbanised areas. Climate propensity analyses further reveal that the province is experiencing an increasingly dry-heat-prone climate, with high temperatures being the dominant factor driving the enhanced hazard associated with dry-heat compound events. This study overcomes the limitations of traditional single-event analyses and provides a more accurate scientific basis for hazard assessment and zonal prevention and control of dry-heat disasters in Liaoning Province. Full article

Logistics involves planning and managing resources to meet customer demands. Its effectiveness depends not only on time and process coordination but also on the performance of logistics operators, whose actions directly affect customer satisfaction. Although operational risks are inherent to logistics, customer-oriented service failures are often overlooked in traditional risk assessment. To address this gap, this study proposes an integrated approach that combines a Pareto Diagram and Failure Mode and Effects Analysis (FMEA) within the ISO 31000 risk assessment framework. This structured method enables the identification and prioritization of logistics failures based on customer complaints, thereby supporting data-driven decision-making and continuous service improvement. Applied to a real-world case in a ceramic production line specializing in tableware manufacturing, the method identified and evaluated key logistics failures; particularly those related to late deliveries and damaged goods. Based on these findings, improvement actions were proposed to reduce the recurrence of these issues. This study contributes a structured, practical, and replicable approach for organizations to introduce risk assessment practices and enhance the service quality of logistics management. This study advances the literature by shifting the focus from internal production failures to customer-driven service risks, offering strategic insights for improving reliability and operational performance. Full article

Urban health, well-being, and equity—core objectives of Sustainable Development Goals (SDGs 3, 10, and 11)—have become key themes in contemporary urban planning research and landscape research. While existing studies focus predominantly on quantitative assessment, environmental exposure, and human mobility, the historical origins of health-oriented urban green space planning remain insufficiently explored. Focusing on Lingnan area as a representative case, this research investigates the emergence of public green space in late Qing cities and its early contributions to urban health and spatial governance. Through a systematic examination of American and British Gardens at the Thirteen Factories in Guangzhou, the planned public green space system of the Shameen concession, and the municipal greening practices of neighboring Hong Kong and Macao, the study further analyzes Zhang Zhidong’s tree-lined boulevard project along Changdi avenue as a key instance of localized institutional adaptation. Drawing on late-Qing and Republican newspapers, nineteenth-century Western travelogs and reports, historical and contemporary studies and photo albums, the study finds the following: (1) the American and British Gardens marked the earliest emergence of health-oriented urban green space in Lingnan area; (2) the systematically planned green space network of the Shameen concession constituted a prototypical form of health-oriented urban green space planning; (3) the botanical gardens, street-tree systems, public parks, and institutionalized management practices in Hong Kong and Macao exerted a strong regional demonstrative influence on Guangzhou; (4) the street-tree planting along Changdi Avenue represented a localized absorption of foreign planning paradigms and marked the institutionalization of municipal greening in Guangzhou. Although these early practices did not yet form a modern healthy city planning framework at that time, they played a crucial role in improving urban sanitation, enhancing public space quality, and shaping urban order. By tracing the historical trajectory from transnational demonstration to local adaptation and institutional consolihdation, this study provides new insights into the historical foundations of health-oriented urban planning in China and contributes a long-term perspective to contemporary debates on healthy cities and nature-based urban interventions. Full article

During the mid-to-late stages of oilfield development, reservoir energy depletion and declining formation pressure coefficients are prevalent challenges. To address the issues of severe fluid loss and extended post-workover fluid recovery periods during conventional operations such as thermal wax removal and pump inspection in low-pressure, waxy wells within a specific block of the Xinjiang Oilfield, a dynamic loss analysis model for workover fluids was developed. Additionally, a wash pressure control valve was engineered to meet the requirements for squeeze killing under abnormal conditions, and an integrated anti-leakage tubing string was designed. This system effectively isolates the workover fluid from the reservoir during interventions, thereby significantly reducing fluid loss and enhancing operational safety. Field applications demonstrate that this technology reduces workover fluid loss by 96% during thermal wax removal and shortens the average post-workover fluid recovery period by 8.7 days after pump inspection. This technology enables rapid restoration of well productivity, lowers operational costs for thermal wax removal and pump inspection, and provides an effective solution for maintaining low-pressure, waxy wells. Full article

Mitral annular disjunction (MAD) is a structural abnormality of the mitral valve increasingly detected with advanced cardiac imaging, particularly cardiac magnetic resonance (CMR). However, the clinical impact of different degrees of disjunction and the lack of standardized measurement criteria remain controversial. This study aimed to describe a series of patients with MAD assessed by CMR and to discuss, in the context of current literature, potential cut-off values that may distinguish physiological from pathological MAD. We retrospectively identified all CMR examinations performed at our institution over a 6-month period in which MAD was visible in at least two cine steady-state free precession (SSFP) projections. For each patient, we recorded MAD extent, presence of mitral valve prolapse/regurgitation, late gadolinium enhancement (LGE) pattern, and main clinical presentation. Nine patients (mean age 57 years; 5 men) were included. Larger MAD distances (>4 mm) were frequently associated with non-ischemic LGE in the basal lateral wall and with valvular abnormalities, whereas smaller disjunctions (≤3 mm) were often observed in patients without significant structural disease. Non-ischemic LGE was present in 6/9 patients, all with MAD > 5 mm. These observations, together with published data, support the hypothesis that small degrees of MAD may represent a frequent anatomical variant, while more extensive disjunction, especially when associated with fibrosis, may indicate a pathological substrate for arrhythmias. Standardized CMR-based criteria and validated MAD cut-off values are needed to improve risk stratification and to incorporate MAD assessment into routine clinical practice. Full article

Alfalfa (Medicago sativa) is a globally important forage crop; however, improvements in its biomass yield have stagnated due to its complex genetic architecture and the costly, labor-intensive phenotyping. This study evaluated the potential of the normalized difference vegetation index (NDVI) to predict biomass yield and enhance selection efficiency in alfalfa breeding programs. Specifically, nineteen alfalfa experimental populations (AEXP 1–19) and one control cultivar (OS 66) were evaluated over two growing seasons in Croatia. NDVI was measured at four development stages using a GreenSeeker sensor and compared with forage yield, dry matter yield, and plant height. NDVI values varied significantly among genotypes, years, and growth stages, ranging from 0.23 to 0.87, and increased consistently from early to late vegetative phases. Strong positive correlations were observed between NDVI and forage yield (r = 0.543–0.843) and plant height (r = 0.537–0.738) at early vegetative, late vegetative, and early bud stages. Conversely, NDVI at the mid-vegetative stage correlated negatively with yield and height (r = –0.622 to –0.794). High-performing populations (AEXP 2, AEXP 15, AEXP 18) also exhibited the highest NDVI values. NDVI is a reliable, non-destructive indicator for early selection of high-yielding alfalfa genotypes, although multi-location validation is advised to confirm its broader applicability. Full article