Search Results (3,660)

River corridor rehabilitation is increasingly expected to deliver coupled outcomes by combining ecological recovery with measurable improvements in human well-being. Cultural ecosystem services (CESs), the non-material benefits people derive from landscapes, are central to this objective but remain difficult to operationalize in securitized transboundary settings, where border governance, uneven mobility, and community histories shape access to rivers and the formation of cultural meanings. This study examines whether perceived conservation effectiveness is associated with higher CES value in the Lower Jordan River Basin (LJRB) and whether this association persists after accounting for the community-group structure. Using survey data from 445 respondents across seven community groups, the perceived CES valuation was assessed through a five-point Cultural Significance rating, analyzed alongside conservation-related and contextual variables. Conservation was measured through perceived conservation impact and self-reported conservation involvement (yes/no). A staged inference design combined group comparisons and multivariable regression with adjustments for the community-group structure and contextual controls. Conservation involvement was not associated with meaningful differences in Cultural Significance. The perceived conservation impact showed a positive association in pooled and simple models but lost independent significance after adjusting for community-group structure, which accounted for much of the explanatory power. These findings indicate that CES valuation in the LJRB is structured more by community-group differences and borderland conditions than by individual conservation participation, underscoring the importance of locally encounterable outcomes and group-tailored engagement strategies in transboundary river planning. Full article

This study developed a Visitor Attraction Quality Checklist to evaluate amenity infrastructure. Seoullo 7017, an elevated linear park located in the Seoul Station area, is the study region. Drawing on the literature on urban regeneration and tourism, as well as amenity-based approaches and the quality evaluation of elevated linear parks, this study develops evaluation criteria that incorporate the physical environment and safety, accessibility and convenience, landscape and identity, and social usage and experience. By applying a longitudinal analysis, on-site qualitative evaluations were conducted between August 2017 and January 2026. The findings show that Seoullo 7017 functions well as a visitor attraction, offering high-standard safety infrastructure, cleanliness, and good esthetic value to accommodate diverse visitors. However, there is a seasonal disparity in cultural programming and limited connections to the surrounding local economy. This study also reveals that rigid planter designs, a lack of tree maintenance, and insufficient shaded areas limit spatial flexibility and visitor comfort. For Seoullo 7017 to pursue sustainability, it must refine its horticultural management, integrate with local businesses, and improve its design. The Visitor Attraction Quality Checklist serves as a longitudinal diagnostic tool for managing elevated urban linear parks as an outcome of global regeneration projects. Full article

The life sciences are currently undergoing a serious transition from the reductive biochemical analysis of dissociated tissues to non-destructive “spatial forensics”. In addition to discovering new molecules, we are moving towards finding out their precise tissue localization and performing in situ interrogation to uncover a biological logic within preserved cellular “neighborhoods”. Our perspective is focused on exploring the spatial imperative, including the structural logic and “neighborhood effects” of the tissue microenvironment, which is a prerequisite to understanding cellular function in normal and in pathological conditions. Beginning with a historical foundation of the origins of histochemistry, dating back to the 19th century with pioneer botanist François-Vincent Raspail, we emphasize the technological metamorphosis, transitioning from classical immunohistochemistry to modern multi- and high-plex spatial multi-omics. A critical evaluation of the current operational landscape has been made, addressing the engineering strategies behind multiplexed immunofluorescence (mIF), the challenges of experimental design in spatial transcriptomics, and the functional symbiosis between targeted and unbiased spatial proteomics. There are many layers of genomic and proteomic information we have to consider in order to unravel the mechanisms underlying body function. If we learn how to combine all this information together, we will be able to better understand how cells communicate with each other and what disrupts their communication, leading to cancer and many other pathologies. It is obvious that by implementing spatial biology tools, it becomes possible to develop new medicines and treat diseases in the most efficient ways. At the same time, we realize that there is an urgent need to learn how to put data pieces together so that they blend seamlessly into a meaningful output, further transitioning spatial biology over time into a routine tool to cure for both common and rare diseases and improve our lives and health. Full article

Background: Resistance to first-line anti-tuberculosis drugs in Mycobacterium tuberculosis represents a significant public health challenge, particularly in high-burden tuberculosis (TB) settings such as Pakistan, where multidrug-resistant (MDR) forms further complicate disease control efforts. Drug resistance is primarily associated with mutations in rpoB, inhA, katG, embA, embB, embC, and pncA. The emergence of novel, region-specific variants underscores the urgent need for integrating genomic surveillance into routine TB diagnostics and regional control programs. This study aimed to identify the spectrum of mutations contributing to first-line drug resistance in MDR-TB isolates from Khyber Pakhtunkhwa, Pakistan. Methods: Whole-genome sequencing was performed on 16 clinical isolates (12 MDR and 4 drug-susceptible) to identify resistance-associated mutations in rpoB, inhA, katG, embA, embB, embC, and pncA. Detected variants were interpreted using the World Health Organization (WHO) mutation catalogue to determine their association with drug resistance. Phylogenetic relationships were inferred using the Bacterial and Viral Bioinformatics Resource Center (BV-BRC) platform. Results: A total of 16 M. tuberculosis isolates were analyzed to evaluate resistance to first-line anti-tuberculosis drugs. In rpoB, 76 distinct variants were identified, including canonical mutations such as Ser450Leu and His445Arg, as well as a potentially novel substitution, Ser431Phe, predicted to confer high-level rifampicin resistance. The katG and inhA genes harbored 24 and 27 mutations, respectively, including well-characterized substitutions such as Ser315Thr and Ala114Glu, which are strongly associated with isoniazid resistance. Mutations in embA and embB were linked to ethambutol resistance, with several variants localized within conserved transmembrane domains critical for drug interaction. Phylogenetic analysis revealed substantial genetic diversity and evidence of local transmission among MDR-TB isolates. Conclusions: This study suggests that the genetic landscape of drug resistance in M. tuberculosis is highly dynamic in endemic regions. The findings highlight the importance of integrating region-specific mutation profiles into molecular diagnostic frameworks to enhance early detection, guide individualized therapeutic interventions, and strengthen strategies aimed at controlling the transmission of MDR-TB. Full article

Accurately mapping agricultural land use in fragmented hilly and mountainous areas is crucial for resource management but is severely challenged by spatial heterogeneity. While high-resolution (HR) images excel at delineating fine parcel boundaries, their limited spectral and temporal information often leads to spectral confusion among diverse agricultural types. To address this limitation, this study proposes a novel spatiotemporal feature-driven geographical zoning method integrating vegetation phenology, topography, and human activity. This zoning strategy decouples the complex global classification task into relatively simple local problems, providing explicit geoscientific constraints for subsequent classification. The proposed method was validated by classifying plain open-field croplands, sloping croplands, terraces, and greenhouses in the hilly and mountainous areas of Beijing using 2 m resolution satellite images. Compared to traditional global classification methods, the proposed zoning-based method increased the overall accuracy from 84.81% to 90.81%, the Kappa coefficient from 0.74 to 0.85, and the Intersection over Union (IoU) from 77.85% to 90.85%. The advantages of geographic zoning were particularly evident in mitigating spatial heterogeneity and enhancing boundary precision. These findings indicate that integrating dynamic geographical zoning as a priori knowledge successfully bridges the gap between HR spatial details and environmental contexts, offering a robust solution for mapping fragmented agricultural landscapes. Full article

The globalization of the food supply chain presents complex challenges for safety assurance within a highly fragmented regulatory landscape. This review synthesizes the frameworks of eight influential jurisdictions—including the European Union (EU), the United States, China, and Codex Alimentarius—to evaluate how legal mandates function as regulatory drivers that guide the evolution of analytical chemistry. By examining legislation on Maximum Residue Limits (MRLs), positive list systems, and method validation guidelines (e.g., SANTE), we demonstrate that strict preventive controls have established chromatography coupled with tandem mass spectrometry (LC/GC-MS/MS) as the universal standard for multi-residue screening. We show that global regulatory fragmentation is not merely an administrative artifact, but is rooted in divergent toxicological philosophies and localized dietary exposure models. This regulatory heterogeneity requires analytical laboratories to adopt a posture of “defensive technological redundancy,” forcing them to continuously optimize targeted methods against the strictest global default limits (e.g., 0.01 mg/kg). We establish that this continuous methodological escalation for ultra-trace quantification has reached practical and operational limits. Consequently, we conclude that the future of food safety testing must transition from static target-list compliance toward adaptable, non-targeted chemical profiling using High-Resolution Mass Spectrometry (HRMS), enabling laboratories to proactively address emerging contaminants, food fraud, and the complexities of modern food matrices. Full article

The once-extensive coastal forested wetlands (CFWs) of the Mississippi River Delta (MRD) are declining under the combined pressures of pervasive hydrologic change, unregulated harvesting, relative water level rise (due to the combination of geological subsidence and sea-level rise—SLR), and climate change. We synthesize here over 50 years of research conducted in the MRD to examine the history of the CFWs and their management, their ecosystem functions and services, and the nature, extent, and severity of ongoing changes. Seedling recruitment failure and increasing salinity levels are the most immediate threats to forest persistence, necessitating management that restores hydrologic function and sediment and nutrient supply to allow seedling survival and minimizes saltwater intrusion. Collectively, the evidence indicates that managed inflows can bolster accretion and sustain forest function, and long-term resilience requires hydrologic restoration at landscape scales coupled with site-level actions that secure recruitment and address local degradation trajectories. These include freshwater and sediment introduction, protection from herbivory, and, in some cases, planting. Our research findings have important implications for worldwide CFWs, and tidal freshwater ecosystems in general, which occur mainly in tropical deltas. Full article

This study investigates the role of glamping within outdoor tourism as a potential tool for preserving and enhancing local landscape identity. Despite its rapid growth, glamping remains weakly defined within regulatory and design frameworks. The paper aims to explore whether a design-oriented approach can redefine glamping as a landscape-based practice rather than a purely market-driven phenomenon, with particular reference to the Italian context. The research adopts a qualitative research-by-design methodology, combining a critical literature review with the development of two pilot projects located in distinct settings: a natural hilly landscape and a rural agricultural context. These projects function as experimental tools to test spatial, ecological, and perceptual design strategies, focusing on settlement density, landscape integration, and experiential quality. The findings identify recurring principles that enable the codification of the glamping–landscape relationship, including low-density configurations, reversibility of structures, respect for existing morphology, and reinforcement of landscape identity. Landscape elements such as topography, vegetation, and visual relationships emerge as primary drivers of design. The study contributes to the discourse by reframing glamping as a landscape design practice, proposing a reversible and context-sensitive model of temporary inhabitation that supports sustainable tourism development. Full article

Traditional villages embody regional culture and local knowledge, yet culture–tourism integration often suffers from a mismatch between resource value and effective transformation. To address this problem, this study proposes a two-dimensional “benefit–obstacle” diagnostic and strategy-matching framework and tests its case-based applicability in Dayuwan Village. First, a cultural landscape gene (CLG) atlas was constructed for the village based on a geo-information coding scheme, covering both tangible and intangible CLGs. Second, a four-dimensional evaluation system was operationalized through five expert judgments and 106 valid on-site questionnaires collected from tourists (n = 67) and residents (n = 39). Criterion weights were determined using an AHP–entropy combination approach, and the comprehensive benefit closeness coefficient was calculated via TOPSIS. Third, an obstacle degree identification model was employed to pinpoint key constraints and derive composite obstacle degrees. Results within the Dayuwan case show that the TOPSIS closeness coefficients of the 17 genes ranged from 0.653 to 0.782 (mean = 0.714), with 4, 6, and 7 genes classified as excellent, good, and medium, respectively; composite obstacle degrees ranged from 0.0228 to 0.1975. In Dayuwan Village, higher obstacle degrees clustered mainly in intangible CLGs, whereas Ming–Qing architecture and frequently practiced folk-cultural genes showed comparatively lower obstacle degrees. The transformation process is constrained by four mechanisms—landscape character protection, economic transformation, social identity, and market demand—with economic transformation constraints being the most prominent. Based on the benefit–obstacle matrix, 17 CLGs were classified into five activation scenarios and matched with corresponding revitalization strategies. This framework links benefit ranking, obstacle diagnosis, and strategy matching, and provides a case-based diagnostic reference for the conservation and culture–tourism integration of villages with comparable heritage conditions, subject to local recalibration of indicators, weights, and thresholds. Full article

Bartter syndrome (BS) represents a group of rare, autosomal recessive renal tubular disorders characterized by hypokalemic hypochloremic metabolic alkalosis, secondary hyperaldosteronism, and normal to low blood pressure. The underlying pathophysiology is primarily driven by defects in critical ion transport proteins or channels localized within the thick ascending limb of the loop of Henle, leading to impaired salt reabsorption. Recent advances in molecular genetics have refined the classification of Bartter syndrome. Current evidence supports SLC12A1, KCNJ1, CLCNKB, BSND, and MAGED2 as the core disease genes within the contemporary BS spectrum, with MAGED2 causing a distinct X-linked transient antenatal form. In contrast, gain-of-function CASR variants, historically labeled “type V Bartter syndrome”, are now more appropriately described as CaSR-associated Bartter-like phenotypes within the broader spectrum of disorders of calcium homeostasis. Despite significant progress, two primary research limitations remain. First, fully elucidating genotype–phenotype correlations and overcoming diagnostic complexities continues to be highly challenging due to substantial phenotypic overlap and genetic heterogeneity. Compounding these diagnostic hurdles is the equally critical challenge of understanding mutation-driven pathogenic mechanisms to develop viable clinical interventions. This review systematically summarizes the current molecular genetic landscape of BS to address these gaps. We highlight the relationships between specific genetic variants and clinical manifestations, delve into molecular pathophysiology including protein misfolding and trafficking defects, and explore emerging therapeutic approaches such as molecular chaperones. By integrating genetic and clinical data, this work aims to provide a comprehensive framework to facilitate precise diagnosis and individualized treatment strategies, ultimately advancing precision medicine in the management of Bartter syndrome. Full article

Vulnerabilities in conventional agri-food supply chains (CAFSCs) necessitate a shift toward resilient, localized production models. Within the Agri-Food 4.0 landscape, urban Controlled-Environment Hydroponic Agriculture (CEHA) systems address these challenges by shortening supply chains and mitigating climate-induced breakdowns. However, structurally aligning Triple Bottom Line (TBL)-oriented stakeholder needs with complex technical specifications remains a critical challenge in sustainable CEHA system design. To address this challenge, the present study proposes a novel framework integrating the Full Consistency Method (FUCOM) and Quality Function Deployment (QFD) within a Finite Interval Type-2 (FIT2) Gaussian fuzzy environment. This approach systematically translates TBL-oriented priorities into precise engineering specifications, mapping 17 stakeholder needs (SNs) to 30 technical design requirements (TDRs) while capturing linguistic uncertainty and hesitation. The findings reveal a clear strategic focus on environmental and social sustainability. Specifically, high product quality, food safety and traceability, consumer acceptance, and minimization of environmental impacts emerge as the primary drivers of CEHA adoption. The QFD translation identifies scalable IoT infrastructure, sensor maintenance and calibration, and AI-enabled decision support as the most critical TDRs. The framework’s reliability and structural robustness were rigorously validated through comprehensive analyses, including Kendall’s W test to confirm expert consensus, alongside a Leave-One-Out (LOO) approach, weight perturbations, and a structural evaluation of TDR intercorrelations. These findings provide a scientifically grounded roadmap for designing sustainable, intelligent urban agricultural systems. Ultimately, this framework offers actionable managerial implications for agribusiness stakeholders to bridge strategic TBL-oriented goals with practical engineering, significantly enhancing Agri-Food 4.0 supply chain resilience. Full article

With the rapid penetration of renewable energy, grid-connected microgrids have become a cornerstone of low-carbon power systems, while also posing major challenges for coordinated scheduling under coupled economic and environmental goals. The resulting dispatch problem is highly nonlinear and high-dimensional, featuring tight operational constraints and conflicting cost–emission trade-offs that often undermine the efficiency and reliability of conventional optimization methods, thereby limiting overall economic productivity. This paper presents an adaptive economic–environmental dispatch framework for grid-connected microgrids formulated as a multi-objective optimization problem that simultaneously minimizes operating cost and environmental protection cost. To navigate the rugged and constrained search landscape, we develop an enhanced metaheuristic termed the Lévy–Triangular Walk Dung Beetle Optimizer (LTWDBO). The LTWDBO integrates (i) chaotic population initialization to improve diversity and feasibility coverage, (ii) a geometry-inspired triangular walk operator to strengthen local exploitation, and (iii) an adaptive Lévy-flight strategy to boost global exploration, achieving a robust exploration–exploitation balance over the entire optimization process, representing a process innovation in metaheuristic-driven dispatch optimization. The proposed method is validated on a representative grid-connected microgrid comprising photovoltaic generation, wind turbines, micro gas turbines, and battery energy storage. Comparative experiments against representative baselines (DBO, WOA, TDBO, and NSGA-II) demonstrate that the LTWDBO achieves consistently better solution quality. Our LTWDBO attains the lowest optimal objective value of 255,718.34 Yuan, compared with 357,702.68 Yuan (DBO), 347,369.28 Yuan (TDBO), and 3,854,359.36 Yuan (WOA). The LTWDBO also yields the best average objective value of 673,842.24 Yuan, an improvement of over 1,001,813.10 Yuan (DBO). Full article

Neglected tropical diseases (NTDs) such as soil-transmitted helminthiasis, lymphatic filariasis, schistosomiasis, leprosy, rabies, and food-borne trematodiasis are endemic in the Philippines. Despite global and national elimination efforts, these six NTDs remain a persistent burden to the poor, those living in Geographically Isolated and Disadvantaged Areas (GIDAs), and other vulnerable groups. This narrative review synthesized data from Field Health Services Information System (FHSIS) reports of the Philippine Department of Health (DOH) from 2020 to 2024, the available literature from electronic databases, and DOH and WHO reports focusing on the challenges, barriers, and gaps in NTD control and elimination in the country. Core challenges include complex epidemiological landscapes, lapses in disease surveillance, infrastructure, and fragmented health care systems. Gaps include access to diagnostics, insufficient funding and human resource training, and scarcity of local studies focusing on endemic NTDs. With these challenges and gaps, this review highlights the need for a real-time feedback loop system in surveillance strategy, community-based interventions, full integration of NTDs in primary health care, and collaboration between government, NGOs and private entities. Addressing these challenges and gaps is key to shifting from control to elimination. Full article

Spaceborne LiDAR systems, such as ICESat-2, provide critical data for global land cover and topography; however, their performance in rugged, vegetated landscapes requires rigorous local validation. This study evaluates the vertical accuracy of ICESat-2 ATL08 terrain height metrics in the complex Turkish Western Black Sea region, utilizing a reference dataset of high-precision terrestrial GNSS measurements. Following strict IQR-based outlier detection and photon density filtering, 1637 spatially matched segments were analyzed. The h_te_best_fit terrain height metric showed the best agreement with the terrestrial GNSS reference data, yielding an RMSE of 3.37 m and a mean bias of −0.42 m, indicating a slight underestimation of the terrain surface. The univariate analysis revealed a strong positive correlation between terrain slope and vertical error, indicating that slope is the prominent degradation factor contributing to pulse broadening. Additionally, dense forest cover was found to limit ground photon retrieval, leading to increased error margins, whereas nighttime acquisitions offered slightly improved precision. These findings suggest that while ATL08 is a valuable topographic source, slope-dependent corrections are essential for applications in mountainous environments. Full article

The viae romanae (Roman roads) were constructed according to precise designs and exceptional engineering techniques, ensuring their strength and durability. They represent an immeasurably important factor in human history. Their impact has been universal, facilitating the movement of people, goods, ideas, beliefs and religions over the centuries. The Via Appia Regina Viarum, built between the end of 4th and 1st centuries BCE, connected Rome to Brundisium, spanning the region of Latium and Apulia. The road initially crossed the coastal plains of the Tyrrhenian Sea (in Latium) before cutting through the reliefs and river valleys of the southern Apennines (in Campania) and finally crossing the regio Apulia et Calabria via Tarentum, to the harbor of Brundisium, along the Adriatic coast. In 2024, the Italian Ministry of Culture proposed the ‘Via Appia Regina Viarum’ for inscription on the Unesco World Heritage List, recognizing its unique and exceptional testimony to Roman civilization. Later that same year, the nomination was accepted, and today, the Via Appia is part of the UNESCO World Heritage List. A significant contribution to this nomination came from the multidisciplinary studies and research conducted along the Via Appia between the ancient cities of Beneventum and Aeclanum in the Campanian Apennine, including: (1) geoarcheological investigation aimed at identifying the ancient path of the road, which was not well documented in the area between Beneventum and Aeclanum; (2) studies focused on cultural and geological heritage along the road and its surrounding landscapes, enhancing the value of the nomination; and (3) the organization of social and cultural events designed to disseminate scientific findings and raise awareness among scientists, students, local and national administrators, local food and wine producers, and the general public. This paper highlights the pivotal role of geoscience at all stages of the project: from preliminary field surveys and mapping of landforms and lithofacies, to targeted field and geophysical surveys, to archaeological excavation and geoarchaeological consideration, and to the dissemination of new data through cultural events. Full article