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Search Results (4,522)

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Keywords = in situ testing

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20 pages, 1811 KiB  
Article
Sentinel-2 Satellite-Derived Bathymetry with Data-Efficient Domain Adaptation
by Christos G. E. Anagnostopoulos, Vassilios Papaioannou, Konstantinos Vlachos, Anastasia Moumtzidou, Ilias Gialampoukidis, Stefanos Vrochidis and Ioannis Kompatsiaris
J. Mar. Sci. Eng. 2025, 13(7), 1374; https://doi.org/10.3390/jmse13071374 - 18 Jul 2025
Abstract
Satellite-derived bathymetry (SDB) enables the efficient mapping of shallow waters such as coastal zones but typically requires extensive local ground truth data to achieve high accuracy. This study evaluates the effectiveness of transfer learning in reducing this requirement while keeping estimation accuracy at [...] Read more.
Satellite-derived bathymetry (SDB) enables the efficient mapping of shallow waters such as coastal zones but typically requires extensive local ground truth data to achieve high accuracy. This study evaluates the effectiveness of transfer learning in reducing this requirement while keeping estimation accuracy at acceptable levels by adapting a deep learning model pretrained on data from Puck Lagoon (Poland) to a new coastal site in Agia Napa (Cyprus). Leveraging the open MagicBathyNet benchmark dataset and a lightweight U-Net architecture, three scenarios were studied and compared: direct inference to Cyprus, site-specific training in Cyprus, and fine-tuning from Poland to Cyprus with incrementally larger subsets of training data. Results demonstrate that fine-tuning with 15 samples reduces RMSE by over 50% relative to the direct inference baseline. In addition, the domain adaptation approach using 15 samples shows comparable performance to the site-specific model trained on all available data in Cyprus. Depth-stratified error analysis and paired statistical tests confirm that around 15 samples represent a practical lower bound for stable SDB, according to the MagicBathyNet benchmark. The findings of this work provide quantitative evidence on the effectiveness of deploying data-efficient SDB pipelines in settings of limited in situ surveys, as well as a practical lower bound for clear and shallow coastal waters. Full article
(This article belongs to the Section Physical Oceanography)
19 pages, 2239 KiB  
Article
Experimental Study on Mechanical Differences Between Prefabricated and Cast-In Situ Tunnel Linings Based on a Load-Structure Model
by Li-Ming Wu, Hong-Kun Li, Feng Gao, Zi-Jian Wang, Bin Zhang, Wen-Jie Luo and Jun-Jie Li
Buildings 2025, 15(14), 2522; https://doi.org/10.3390/buildings15142522 - 18 Jul 2025
Abstract
With the accelerated development of urban underground spaces, prefabricated tunnel linings have become a research focus due to their advantages in construction efficiency and cost effectiveness. However, issues such as stress concentration at joints and insufficient overall stability hinder their broader application. This [...] Read more.
With the accelerated development of urban underground spaces, prefabricated tunnel linings have become a research focus due to their advantages in construction efficiency and cost effectiveness. However, issues such as stress concentration at joints and insufficient overall stability hinder their broader application. This study investigates a cut-and-cover prefabricated tunnel project in the Chongqing High-Tech Zone through scale model tests and numerical simulations to systematically compare the mechanical behaviors of cast-in situ linings and three-segment prefabricated linings under surrounding rock loads. The experimental results show that the ultimate bearing capacity of the prefabricated lining is 15.3% lower than that of the cast-in situ lining, with asymmetric failure modes and cracks concentrated near joint regions. Numerical simulations further reveal the influence of joint stiffness on structural performance: when the joint stiffness is 30 MN·m/rad, the bending moment of the segmented lining decreases by 37.7% compared to the cast-in situ lining, while displacement increments remain controllable. By optimising joint pre-tightening forces and stiffness parameters, prefabricated linings can achieve stability comparable to cast-in situ structures while retaining construction efficiency. This research provides theoretical and technical references for the design and construction of open-cut prefabricated tunnel linings. Full article
(This article belongs to the Section Building Structures)
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14 pages, 3909 KiB  
Article
Demonstrating In Situ Formation of Globular Microstructure for Thixotropic Printing of EN AW-4043 Aluminum Alloy
by Silvia Marola and Maurizio Vedani
Metals 2025, 15(7), 804; https://doi.org/10.3390/met15070804 - 17 Jul 2025
Abstract
This study explores the feasibility of generating a globular microstructure in situ during the thixotropic 3D printing of the EN AW-4043 alloy, starting from a conventional cold-rolled wire. Thermodynamic simulations using Thermo-Calc software were first conducted to identify the semi-solid processing window of [...] Read more.
This study explores the feasibility of generating a globular microstructure in situ during the thixotropic 3D printing of the EN AW-4043 alloy, starting from a conventional cold-rolled wire. Thermodynamic simulations using Thermo-Calc software were first conducted to identify the semi-solid processing window of the alloy, based on the evolution of liquid and solid fractions as a function of temperature. Guided by these results, thermal treatments were performed on cold-rolled wires to promote the formation of a globular microstructure. A laboratory-scale printing head prototype was then designed and built to test continuous heating and deposition conditions representative of a thixotropic additive manufacturing process. The results showed that a globular microstructure could be achieved in the cold-rolled EN AW-4043 wires by heating them at 590 °C for 5 min in a static muffle furnace. A similar effect was observed when continuously heating the wire while it flowed through the heated printing head. Preliminary deposition tests confirmed the viability of this approach and demonstrated that thixotropic 3D printing of EN AW-4043 alloy is achievable without the need for pre-globular feedstock. Full article
(This article belongs to the Section Additive Manufacturing)
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24 pages, 3120 KiB  
Article
Asymbiotic Seed Germination and In Vitro Propagation of the Thai Rare Orchid Species; Eulophia bicallosa (D.Don) P.F.Hunt & Summerh.
by Thanakorn Wongsa, Jittra Piapukiew, Kanlaya Kuenkaew, Chatchaya Somsanook, Onrut Sapatee, Julaluk Linjikao, Boworn Kunakhonnuruk and Anupan Kongbangkerd
Plants 2025, 14(14), 2212; https://doi.org/10.3390/plants14142212 - 17 Jul 2025
Abstract
An efficient in vitro propagation protocol for Eulophia bicallosa was developed using asymbiotic seed germination and protocorm proliferation. The effect of light on seed germination and development was evaluated on Vacin and Went (VW) medium under five conditions: darkness, white, green, red, and [...] Read more.
An efficient in vitro propagation protocol for Eulophia bicallosa was developed using asymbiotic seed germination and protocorm proliferation. The effect of light on seed germination and development was evaluated on Vacin and Went (VW) medium under five conditions: darkness, white, green, red, and blue light for 24 weeks. Blue and red light significantly accelerated seed development, allowing progression to stage 5 within 24 weeks. For protocorm proliferation, six semi-solid culture media were tested. Half-strength Murashige and Skoog (½MS) medium yielded the best results after 8 weeks, producing the highest numbers of shoots (1.0), leaves (1.1), and roots (4.2) per protocorm, with 100% survival. The effects of organic additives were also evaluated using coconut water and potato extract. A combination of 200 mL L−1 coconut water and 50 g L−1 potato extract enhanced shoot formation (1.7 shoots), while 150 mL L−1 coconut water with 50 g L−1 potato extract increased both leaf (1.9) and root (8.8) numbers. The effects of cytokinins (benzyladenine (BA), kinetin (6-furfurylaminopurine), and thidiazuron (TDZ)) and auxins (indole-3-acetic acid (IAA), α-naphthalene acetic acid (NAA), indole-3-butyric acid (IBA), and 2,4-dichlorophenoxyacetic acid (2,4-D)) were investigated using ½MS medium supplemented with each plant growth regulator individually at concentrations of 0, 0.1, 0.5, 1.0, and 2.0 mg L−1. Among the cytokinins, 0.1 mg L−1 BA produced the highest survival rate (96%), while 1.0 mg L−1 BA induced the greatest shoot formation (93%, 2.3 shoots). Among the auxins, 0.1 mg L−1 IAA resulted in the highest survival (96%), and 1.0 mg L−1 IAA significantly enhanced root induction (4.2 roots per protocorm). Acclimatization in pots containing a 1:1:1 (v/v) mixture of pumice, sand, and soil resulted in 100% survival. This protocol provides a reliable and effective approach for the mass propagation and ex situ conservation of E. bicallosa. Full article
(This article belongs to the Section Plant Development and Morphogenesis)
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25 pages, 7522 KiB  
Article
Quantitative Estimation of Vegetation Carbon Source/Sink and Its Response to Climate Variability and Anthropogenic Activities in Dongting Lake Wetland, China
by Mengshen Guo, Nianqing Zhou, Yi Cai, Xihua Wang, Xun Zhang, Shuaishuai Lu, Kehao Liu and Wengang Zhao
Remote Sens. 2025, 17(14), 2475; https://doi.org/10.3390/rs17142475 - 16 Jul 2025
Viewed by 104
Abstract
Wetlands are critical components of the global carbon cycle, yet their carbon sink dynamics under hydrological fluctuations remain insufficiently understood. This study employed the Carnegie-Ames-Stanford Approach (CASA) model to estimate the net ecosystem productivity (NEP) of the Dongting Lake wetland and explored the [...] Read more.
Wetlands are critical components of the global carbon cycle, yet their carbon sink dynamics under hydrological fluctuations remain insufficiently understood. This study employed the Carnegie-Ames-Stanford Approach (CASA) model to estimate the net ecosystem productivity (NEP) of the Dongting Lake wetland and explored the spatiotemporal dynamics and driving mechanisms of carbon sinks from 2000 to 2022, utilizing the Theil-Sen median trend, Mann-Kendall test, and attribution based on the differentiating equation (ADE). Results showed that (1) the annual mean spatial NEP was 50.24 g C/m2/a, which first increased and then decreased, with an overall trend of −1.5 g C/m2/a. The carbon sink was strongest in spring, declined in summer, and shifted to a carbon source in autumn and winter. (2) Climate variability and human activities contributed +2.17 and −3.73 g C/m2/a to NEP, respectively. Human activities were the primary driver of carbon sink degradation (74.30%), whereas climate change mainly promoted carbon sequestration (25.70%). However, from 2000–2011 to 2011–2022, climate change shifted from enhancing to limiting carbon sequestration, mainly due to the transition from water storage and lake reclamation to ecological restoration policies and intensified climate anomalies. (3) NEP was negatively correlated with precipitation and water level. Land use adjustments, such as forest expansion and conversion of cropland and reed to sedge, alongside maintaining growing season water levels between 24.06~26.44 m, are recommended to sustain and enhance wetland carbon sinks. Despite inherent uncertainties in model parameterization and the lack of sufficient in situ flux validation, these findings could provide valuable scientific insights for wetland carbon management and policy-making. Full article
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18 pages, 11712 KiB  
Article
Measuring Transient Friction Coefficient Affected by Plastic Heat Generation Using a Warm Ring Compression Test with an In Situ Measurement System Measuring Ring Expansion Velocity
by Alireza Soleymanipoor, Tomoyoshi Maeno, Kosuke Tosaka, Masato Kakudo, Kazuhito Takahashi, Motoki Yanagisawa and Osami Tsukamoto
J. Manuf. Mater. Process. 2025, 9(7), 241; https://doi.org/10.3390/jmmp9070241 - 16 Jul 2025
Viewed by 174
Abstract
Frictional conditions at the workpiece–die interface are critical in metal forming, as significant plastic deformation generates heat that affects lubricant performance. Understanding lubricant behavior, especially its influence on friction under elevated temperatures, is essential for optimizing forming processes and meeting ecological demands. While [...] Read more.
Frictional conditions at the workpiece–die interface are critical in metal forming, as significant plastic deformation generates heat that affects lubricant performance. Understanding lubricant behavior, especially its influence on friction under elevated temperatures, is essential for optimizing forming processes and meeting ecological demands. While the conventional ring compression test evaluates friction through inner diameter changes, it becomes unreliable when friction is transient. In this study, a warm ring compression test incorporating an in situ measurement system is proposed to evaluate the transient frictional behavior of lubricants under temperature rise due to plastic deformation. Results show that at T = 50 °C and 150 °C, the friction coefficient increases notably with the compression ratio, whereas at T = 100 °C, it remains relatively stable. This stability is likely due to the optimal performance of the chlorinated base lubricant at 100 °C, where boundary lubrication is most effective. At T = 50 °C, the additive activation is insufficient, and at T = 150 °C, thermal degradation may reduce its effectiveness. Finite element simulations using the transient friction coefficient reproduce the deformed ring cross-section with high accuracy, while those using constant friction values show less agreement. Full article
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12 pages, 1899 KiB  
Article
Climatic Factors in Beechnut Regeneration: From Seed Quality to Germination
by Ernesto C. Rodríguez-Ramírez and Beatriz Argüelles-Marrón
Stresses 2025, 5(3), 44; https://doi.org/10.3390/stresses5030044 - 16 Jul 2025
Viewed by 51
Abstract
Masting, or the synchronous and intermittent production of seeds, can have profound consequences for Tropical Montane Cloud Forest (TMCF) tree populations and the trophic webs that depend on their mass flowering and seeds. Over the past 80 years, the importance of Fagus mexicana [...] Read more.
Masting, or the synchronous and intermittent production of seeds, can have profound consequences for Tropical Montane Cloud Forest (TMCF) tree populations and the trophic webs that depend on their mass flowering and seeds. Over the past 80 years, the importance of Fagus mexicana Martínez (Mexican beech) masting has become apparent in terms of conservation and management, promoting regeneration, and conserving endangered tree species, as well as the conscientious development of edible beechnuts as a non-timber forest product. The establishment of the relict-endemic Mexican beech is unknown, and several microenvironmental factors could influence natural regeneration. Thus, this study was conducted in two well-preserved Mexican beech forests to assess the influence of light incidence and soil moisture on the natural germination and seedling establishment of beeches. During two masting years (2017 and 2024), we assessed in situ beechnut germination and establishment. We tested the effect of the microenvironment of the oldest beeches on beechnut germination and seedling establishment. Our study highlights the complexity of the microenvironment of old beeches influencing the early stages of establishment and provides insights into possible conservation actions aimed at mitigating the impact of environmental change and humans. Full article
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17 pages, 3482 KiB  
Article
Chinese Pangolins in China Demonstrate Regional Differences in Burrow Habitat Selection
by Dongling Liang, Xinrui Tang, Yilong Chen, Fei Xi, Shibao Wu and Fuhua Zhang
Animals 2025, 15(14), 2093; https://doi.org/10.3390/ani15142093 - 16 Jul 2025
Viewed by 96
Abstract
Knowledge of the habitat characteristics of endangered species is an important basis for in situ conservation, release-site selection, and habitat modification. Although the Chinese pangolin (Manis pentadactyla) is one of the world’s most endangered species, little is known about its habitat [...] Read more.
Knowledge of the habitat characteristics of endangered species is an important basis for in situ conservation, release-site selection, and habitat modification. Although the Chinese pangolin (Manis pentadactyla) is one of the world’s most endangered species, little is known about its habitat preferences, and the results of past studies differ greatly. To clarify the habitat characteristics of the Chinese pangolin, we conducted habitat surveys in Guangdong, Jiangxi, and Zhejiang provinces of China using the transect method. A total of 520 burrow sites of Chinese pangolins were recorded in three study areas. The resulting data were analyzed using a generalized additive model, principal coordinate analysis, and Kruskal–Wallis tests. Nine ecological factors (elevation, slope, soil type, canopy coverage, surface coverage, number of trees, number of logs, tree diameter at breast height, and distance to a settlement) were found to affect pangolins’ distribution. Burrows were preferentially distributed at elevations of 50–150 m (62.3%), in silty soil (88.1%), on 20–40° slopes (83.3%), within young and medium-aged broadleaved forests with a canopy coverage exceeding 70% (65.8%), and close to water (less than 300 m). Among the three study regions, pangolin habitats differed significantly in seven environmental factors: elevation, canopy coverage, surface coverage, number of trees, distance to water, distance to a road, and distance to a settlement. Our findings imply that the Chinese pangolin appears to tolerate a broad range of ecological characteristics; however, food resources may be the key factor affecting its habitat selection, and other factors may indirectly affect its distribution by affecting food abundance. Finally, aside from hunting, a low level of human disturbance does not affect the presence of this species. Full article
(This article belongs to the Section Ecology and Conservation)
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13 pages, 2193 KiB  
Article
In Situ Electrochemical Atomic Force Microscopy Study of Interfacial Reactions on a Graphite Negative Electrode for Magnesium-Ion Batteries
by Sungjae Yoon, Paul Maldonado Nogales, Sangyup Lee, Seunga Yang and Soon-Ki Jeong
Int. J. Mol. Sci. 2025, 26(14), 6793; https://doi.org/10.3390/ijms26146793 - 15 Jul 2025
Viewed by 152
Abstract
The cointercalation of solvated Mg2+ ions into graphite has typically been considered challenging because of concerns regarding the instability of the electrolyte and the potential for structural degradation. However, recent developments in electrolyte design suggest that this process may be reversible under [...] Read more.
The cointercalation of solvated Mg2+ ions into graphite has typically been considered challenging because of concerns regarding the instability of the electrolyte and the potential for structural degradation. However, recent developments in electrolyte design suggest that this process may be reversible under appropriate conditions. In this study, the interfacial behavior of graphite in a magnesium-ion system was investigated using in situ electrochemical atomic force microscopy. Electrochemical tests in a triglyme-based electrolyte revealed a reversible capacity of 158 mAh g−1, attributed to the insertion of triglyme-solvated Mg2+ ions. Real-time surface imaging of highly oriented pyrolytic graphite revealed the formation of a passivating surface film during the initial cycle, along with nanoscale hill-like (~1 nm) and blister-like (~5 nm) structures, which were partially reversible and showed good correlation with the redox peaks observed in the cyclic voltammetry experiments, suggesting that the surface film enables Mg2+ transport while mitigating electrolyte decomposition. These findings demonstrate that stable co-intercalation of solvated Mg2+ ions is achievable in the early cycles in graphite and highlight the importance of interfacial engineering and solvation structures in the development of magnesium-ion batteries. Full article
(This article belongs to the Section Physical Chemistry and Chemical Physics)
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22 pages, 7389 KiB  
Article
FeCo-LDH/CF Cathode-Based Electrocatalysts Applied to a Flow-Through Electro-Fenton System: Iron Cycling and Radical Transformation
by Heng Dong, Yuying Qi, Zhenghao Yan, Yimeng Feng, Wenqi Song, Fengxiang Li and Tao Hua
Catalysts 2025, 15(7), 685; https://doi.org/10.3390/catal15070685 - 15 Jul 2025
Viewed by 92
Abstract
In this investigation, a hierarchical FeCo-layered double hydroxide (FeCo-LDH) electrochemical membrane material was prepared by a simple in situ hydrothermal method. The prepared material formed a 3D honeycomb-structured FeCo-LDH-modified carbon felt (FeCo-LDH/CF) catalytic layer with uniform open pores on a CF substrate with [...] Read more.
In this investigation, a hierarchical FeCo-layered double hydroxide (FeCo-LDH) electrochemical membrane material was prepared by a simple in situ hydrothermal method. The prepared material formed a 3D honeycomb-structured FeCo-LDH-modified carbon felt (FeCo-LDH/CF) catalytic layer with uniform open pores on a CF substrate with excellent catalytic activity and was served as the cathode in a flow-through electro-Fenton (FTEF) reactor. The electrocatalyst demonstrated excellent treatment performance (99%) in phenol simulated wastewater (30 mg L−1) under the optimized operating conditions (applied voltage = 3.5 V, pH = 6, influent flow rate = 15 mL min−1) of the FTEF system. The high removal rate could be attributed to (i) the excellent electrocatalytic oxidation performance and low interfacial charge transfer resistance of the FeCo-LDH/CF electrode as the cathode, (ii) the ability of the synthesized FeCo-LDH to effectively promote the conversion of H2O2 to •OH under certain conditions, and (iii) the flow-through system improving the mass transfer efficiency. In addition, the degradation process of pollutants within the FTEF system was additionally illustrated by the •OH dominant ROS pathway based on free radical burst experiments and electron paramagnetic resonance tests. This study may provide new insights to explore reaction mechanisms in FTEF systems. Full article
(This article belongs to the Special Issue Environmentally Friendly Catalysis for Green Future)
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27 pages, 11290 KiB  
Article
Experimental Study on Compressive Capacity Behavior of Helical Anchors in Aeolian Sand and Optimization of Design Methods
by Qingsheng Chen, Wei Liu, Linhe Li, Yijin Wu, Yi Zhang, Songzhao Qu, Yue Zhang, Fei Liu and Yonghua Guo
Buildings 2025, 15(14), 2480; https://doi.org/10.3390/buildings15142480 - 15 Jul 2025
Viewed by 125
Abstract
The compressive capacity of helical anchors constitutes a pivotal performance parameter in geotechnical design. To precisely predict the compressive bearing behavior of helical anchors in aeolian sand, this study integrates in situ testing with finite element numerical analysis to systematically elucidate the non-linear [...] Read more.
The compressive capacity of helical anchors constitutes a pivotal performance parameter in geotechnical design. To precisely predict the compressive bearing behavior of helical anchors in aeolian sand, this study integrates in situ testing with finite element numerical analysis to systematically elucidate the non-linear evolution of its load-bearing mechanisms. The XGBoost algorithm enabled the rigorous quantification of the governing geometric features of compressive capacity, culminating in a computational framework for the bearing capacity factor (Nq) and lateral earth pressure coefficient (Ku). The research findings demonstrate the following: (1) Compressive capacity exhibits significant enhancement with increasing helix diameter yet displays limited sensitivity to helix number. (2) Load–displacement curves progress through three distinct phases—initial quasi-linear, intermediate non-linear, and terminal quasi-linear stages—under escalating pressure. (3) At embedment depths of H < 5D, tensile capacity diminishes by approximately 80% relative to compressive capacity, manifesting as characteristic shallow anchor failure patterns. (4) When H ≥ 5D, stress redistribution transitions from bowl-shaped to elliptical contours, with ≤10% divergence between uplift/compressive capacities, establishing 5D as the critical threshold defining shallow versus deep anchor behavior. (5) The helix spacing ratio (S/D) governs the failure mode transition, where cylindrical shear (CS) dominates at S/D ≤ 4, while individual bearing (IB) prevails at S/D > 4. (6) XGBoost feature importance analysis confirms internal friction angle, helix diameter, and embedment depth as the three parameters exerting the most pronounced influence on capacity. (7) The proposed computational models for Nq and Ku demonstrate exceptional concordance with numerical simulations (mean deviation = 1.03, variance = 0.012). These outcomes provide both theoretical foundations and practical methodologies for helical anchor engineering in aeolian sand environments. Full article
(This article belongs to the Section Building Structures)
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10 pages, 1099 KiB  
Communication
Fluorescent In Situ Hybridization Testing Allows the Diagnosis of NRG1 Gene Fusions in Lung and Pancreas Cancers with No Other Identified Oncogenic Driver
by Clara Bastard, Charline Caumont, Laura Samaison, Isabelle Quintin-Roué, Laurent Doucet, Pascale Marcorelles, Cédric Le Maréchal, Jean-Philippe Merlio, David Cappellen and Arnaud Uguen
Cancers 2025, 17(14), 2347; https://doi.org/10.3390/cancers17142347 - 15 Jul 2025
Viewed by 98
Abstract
Some pancreatic ductal-type (PDADK) and lung adenocarcinomas (LADK) lacking other molecular drivers are reported to harbor NRG1 fusions as potential novel therapeutic targets. We investigated the feasibility of a fluorescent in situ hybridization (FISH)-based diagnosis of NRG1 fusions in a case series of [...] Read more.
Some pancreatic ductal-type (PDADK) and lung adenocarcinomas (LADK) lacking other molecular drivers are reported to harbor NRG1 fusions as potential novel therapeutic targets. We investigated the feasibility of a fluorescent in situ hybridization (FISH)-based diagnosis of NRG1 fusions in a case series of PDADK and LADK lacking other identified oncogenic drivers. First, among a case series of PDADK, KRAS analyses (PCR followed in PCR-negative cases by RNA sequencing—RNAseq) found 27/162 (16.7%) KRAS wild-type cases, among which 1/162 (0.6%) NRG1 fusion was diagnosed using FISH. Secondly, among a case series of LDAK, 191/446 (42.8%) cases had no molecular alterations in EGFR, KRAS, BRAF, HER2, MET, ALK, ROS1 and RET according to NGS and FISH analyses and, among them, 4/446 (0.9%) cases had NRG1 fusions using FISH. Finally, four additional cases out of the two previously mentioned cases series (1 PDADK and 3 LADK) with NRG1 fusions diagnosed by first-line RNAseq were also concluded as NRG1 FISH-positive. The NRG1 FISH tests for the nine NRG1 FISH-positive cases resulted in 50% to 80% of positive tumor nuclei, all with single 3′-NRG1 FISH signals. In our series, of the 22 cases analyzed with both NRG1 FISH (positivity criterion of at least 15% of tumor nuclei with a split between the 5′- and the 3′- parts of the probes and/or isolated single 3′-NRG1 signal) and RNAseq, 17 cases were FISH– RNAseq– and 5 cases were FISH+ RNAseq+ (no FISH+ RNAseq– or FISH– RNAseq+ cases in our study) resulting in 100% sensibility and specificity for the NRG1 FISH test. In the case of no access to RNAseq, NRG1 FISH consists of a valuable tool searching for NRG1 fusions in patients with advanced cancers. Full article
(This article belongs to the Section Cancer Biomarkers)
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26 pages, 9003 KiB  
Article
A Pilot-Scale Gasifier Freeboard Equipped with Catalytic Filter Candles for Particulate Abatement and Tar Conversion: 3D-CFD Simulations and Experimental Tests
by Alessandra Tacconi, Pier Ugo Foscolo, Sergio Rapagnà, Andrea Di Carlo and Alessandro Antonio Papa
Processes 2025, 13(7), 2233; https://doi.org/10.3390/pr13072233 - 12 Jul 2025
Viewed by 322
Abstract
This work deals with the catalytic steam reforming of raw syngas to increase the efficiency of coupling gasification with downstream processes (such as fuel cells and catalytic chemical syntheses) by producing high-temperature, ready-to-use syngas without cooling it for cleaning and conditioning. Such a [...] Read more.
This work deals with the catalytic steam reforming of raw syngas to increase the efficiency of coupling gasification with downstream processes (such as fuel cells and catalytic chemical syntheses) by producing high-temperature, ready-to-use syngas without cooling it for cleaning and conditioning. Such a combination is considered a key point for the future exploitation of syngas produced by steam gasification of biogenic solid fuel. The design and construction of an integrated gasification and gas conditioning system were proposed approximately 20 years ago; however, they still require further in-depth study for practical applications. A 3D model of the freeboard of a pilot-scale, fluidized bed gasification plant equipped with catalytic ceramic candles was used to investigate the optimal operating conditions for in situ syngas upgrading. The global kinetic parameters for methane and tar reforming reactions were determined experimentally. A fluidized bed gasification reactor (~5 kWth) equipped with a 45 cm long segment of a fully commercial filter candle in its freeboard was used for a series of tests at different temperatures. Using a computational fluid dynamics (CFD) description, the relevant parameters for apparent kinetic equations were obtained in the frame of a first-order reaction model to describe the steam reforming of key tar species. As a further step, a CFD model of the freeboard of a 100 kWth gasification plant, equipped with six catalytic ceramic candles, was developed in ANSYS FLUENT®. The composition of the syngas input into the gasifier freeboard was obtained from experimental results based on the pilot-scale plant. Simulations showed tar catalytic conversions of 80% for toluene and 41% for naphthalene, still insufficient compared to the threshold limits required for operating solid oxide fuel cells (SOFCs). An overly low freeboard temperature level was identified as the bottleneck for enhancing gas catalytic conversions, so further simulations were performed by injecting an auxiliary stream of O2/steam (50/50 wt.%) through a series of nozzles at different heights. The best simulation results were obtained when the O2/steam stream was fed entirely at the bottom of the freeboard, achieving temperatures high enough to achieve a tar content below the safe operating conditions for SOFCs, with minimal loss of hydrogen content or LHV in the fuel gas. Full article
(This article belongs to the Section Chemical Processes and Systems)
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17 pages, 3709 KiB  
Article
In Situ Gel-Forming System for the Removal of Ferruginous Deposits on Nanhai I Shipwreck
by Jianrui Zha, Ruyi Wang, Jing Du, Naisheng Li and Xiangna Han
Gels 2025, 11(7), 543; https://doi.org/10.3390/gels11070543 - 12 Jul 2025
Viewed by 134
Abstract
The removal of iron deposits on shipwreck surfaces by mechanical cleaning is labour-intensive work. This study develops an in situ gel and peeling cleaning method, utilising a carboxymethyl chitosan/tannic acid (CMCS/TA) colloidal solution spray on the surface of ferruginous deposits, promoting their removal [...] Read more.
The removal of iron deposits on shipwreck surfaces by mechanical cleaning is labour-intensive work. This study develops an in situ gel and peeling cleaning method, utilising a carboxymethyl chitosan/tannic acid (CMCS/TA) colloidal solution spray on the surface of ferruginous deposits, promoting their removal by adhesion, chelation, and electrostatic bonding processes. The investigation confirmed that the CMTA-2 sample exhibited a sprayable viscosity of 263 mPa/s, the largest single removal thickness of 1.01 mm, a significant reduction in the fe/s atomic ratio by 2.53 units, and enhanced the deposit removal homogeneity. The field testing of the Nanhai I cultural relic showed a 14.37% reduction in iron concentration and a significant decrease in red colour (Δa* = 4.36). The synergistic mechanism involves TA chelating Fe2+/Fe3+ ions, while the CMCS gel network facilitates interfacial adhesion and mechanical peeling, hence promoting efficient and controllable cleaning. Full article
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15 pages, 6918 KiB  
Article
Temperature-Responsive and Self-Healing Hydrogel: A Novel Approach to Combat Postoperative Adhesions
by Yujia Zhan, Xueshan Zhao, Changyuan He, Siwei Bi, Ruiqi Liu, Jun Gu and Bin Yan
Polymers 2025, 17(14), 1925; https://doi.org/10.3390/polym17141925 - 12 Jul 2025
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Abstract
Postoperative adhesions are a prevalent complication following abdominal surgeries, often leading to significant clinical challenges. This study introduces an innovative solution utilizing a polyethylene glycol (PEG)-based triblock copolymer to form an injectable, self-healing hydrogel aimed at preventing these adhesions. The hydrogel, formulated with [...] Read more.
Postoperative adhesions are a prevalent complication following abdominal surgeries, often leading to significant clinical challenges. This study introduces an innovative solution utilizing a polyethylene glycol (PEG)-based triblock copolymer to form an injectable, self-healing hydrogel aimed at preventing these adhesions. The hydrogel, formulated with temperature-responsive and self-healing properties through the incorporation of poly (N-isopropyl acrylamide) (PNIPAM) and anion–pi interactions, was synthesized using reversible addition–fragmentation chain transfer (RAFT) polymerization. The hydrogel’s physical properties, biocompatibility, hemostatic effect, and anti-adhesive capabilities were rigorously tested through in vitro and in vivo experiments involving rat models. It demonstrated excellent biocompatibility, effective tissue adhesion, and robust hemostatic properties. Most notably, it exhibited significant anti-adhesive effects in a rat abdominal wall–cecum model, reducing adhesion formation effectively compared to controls. The PEG-based injectable hydrogel presents a promising approach for postoperative adhesion prevention. Its ability to gel in situ triggered by body heat, coupled with its self-healing properties, provides a substantial advantage in clinical settings, indicating its potential utility as a novel anti-adhesion material. Full article
(This article belongs to the Section Smart and Functional Polymers)
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