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Keywords = in situ framing

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18 pages, 1608 KB  
Article
In Situ Analysis of Electrodermal Activity from Students Learning from Large Language Models Versus Curated Texts
by Kenneth Y. T. Lim, Yue Heng Wong, Duc Nam Tran, Edrik K. X. Lee, Thien Minh Tuan Nguyen, Duc Minh Anh Nguyen and Alan J. H. Tan
Brain Sci. 2026, 16(2), 153; https://doi.org/10.3390/brainsci16020153 - 29 Jan 2026
Viewed by 247
Abstract
Background: this paper reports an investigation into the cognitive and emotional states of adolescents while learning from an LLM. It seeks to address a relative dearth in empirical evidence which might otherwise facilitate informed decisions being made by curriculum designers, school leaders and [...] Read more.
Background: this paper reports an investigation into the cognitive and emotional states of adolescents while learning from an LLM. It seeks to address a relative dearth in empirical evidence which might otherwise facilitate informed decisions being made by curriculum designers, school leaders and policy makers regarding the use of Generative AI, amidst the wider discourse about the effectiveness of AI in teaching and learning. Methods: in this paper, we analyze electrodermal activity (EDA) in the context of students’ scholastic engagement using LLMs in comparison to curated texts. In our 27-min-long experiment, we recorded the EDA of participants learning from both learning methods, for 8 min each. A quiz was also conducted to assess the effectiveness of the learning method. We collected 23 samples of EDA from the experiment, and 42 samples of quiz results. Results: we have found that learning with an LLM results in greater Skin Conductance Response (p = 0.09404), which is linked to more positive emotional valence, and lower Skin Conductance Level (p = 0.09473), which is linked to lower cognitive load, compared to curated texts. We also discovered that learning with an LLM correlates to a higher quiz result (p = 0.02053). While this suggests that learning and absorbing information with an LLM could be more effective than curated texts, results from self-reported data indicate that there are few perceived differences between the effectiveness of LLM and curated texts. Conclusions: this exploratory and preliminary study revealed empirical insights between LLM usage and learning effectiveness in situ via physiological indicators, in contrast to prior work that has adopted post hoc frames over the medium- to long-term. Full article
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26 pages, 3158 KB  
Article
Comparative Life Cycle Assessment of Concrete Elements: Cast In Situ, Semi-Precast and Precast
by Kurt Meilak, Ruben Paul Borg and Muhammad Ali Musarat
Buildings 2026, 16(1), 146; https://doi.org/10.3390/buildings16010146 - 28 Dec 2025
Viewed by 521
Abstract
The construction industry is moving forward with the aim to maintain process sustainability. In this aspect, the role of life cycle assessment is essential in determining the appropriate material or method. Hence, this study aims to compare the environmental impacts of a concrete [...] Read more.
The construction industry is moving forward with the aim to maintain process sustainability. In this aspect, the role of life cycle assessment is essential in determining the appropriate material or method. Hence, this study aims to compare the environmental impacts of a concrete frame office structure, built with different construction techniques, including cast in situ, precast, and semi-precast methods of construction. The analysis adopts the CML 2001 characterisation method to present and compare the environmental impacts from cradle to gate and, therefore, the extraction and production of raw materials, manufacturing, and construction phases of these technologies. Results show that the total energy consumption for the three methods of construction is similar, with a variation of less than 5%. The highest energy consumption phase is associated with the extraction and production of cement. In fact, the precast and semi-precast methods were found to have almost 30% higher global warming potential (CO2 equation) than the cast in situ method per functional unit due to the utilisation of higher cement content in the mix. Hence, the environmental impacts associated with each phase will help the concrete construction industry to develop and improve its efficiency while adopting more sustainable measures. Full article
(This article belongs to the Section Building Structures)
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43 pages, 6411 KB  
Article
The Art Nouveau Path: Valuing Urban Heritage Through Mobile Augmented Reality and Sustainability Education
by João Ferreira-Santos and Lúcia Pombo
Heritage 2026, 9(1), 4; https://doi.org/10.3390/heritage9010004 - 23 Dec 2025
Viewed by 356
Abstract
Cultural heritage is framed as a living resource for citizenship and education, although evidence on how in situ augmented reality can cultivate sustainability competences remains limited. This study examines the Art Nouveau Path, a location-based mobile augmented reality game across eight points [...] Read more.
Cultural heritage is framed as a living resource for citizenship and education, although evidence on how in situ augmented reality can cultivate sustainability competences remains limited. This study examines the Art Nouveau Path, a location-based mobile augmented reality game across eight points of interest in Aveiro, Portugal, aligned with the GreenComp framework. Within a design-based research case study, the analysis integrates repeated cross-sectional student questionnaires (S1-PRE N = 221; S2-POST N = 439; S3-FU N = 434), anonymized gameplay logs from 118 collaborative groups, and 24 teacher field observations (T2-OBS), using quantitative summaries with reflexive thematic analysis. References to heritage preservation in students’ sustainability conceptions rose from 28.96% at baseline to 61.05% immediately after gameplay, remaining above baseline at follow-up (47.93%). Augmented reality items were answered more accurately than non- augmented reality items (81% vs. 73%) and involved longer on-site exploration (+10.17 min). Triangulated evidence indicates that augmented reality and multimodality amplified attention to architectural details and prompted debates about authenticity. Built heritage, mobilized through lightweight augmented reality within a digital teaching and learning ecosystem, can serve as an effective context for Education for Sustainable Development, strengthening preservation literacy and civic responsibility and generating interoperable cultural traces for future reuse. Full article
(This article belongs to the Special Issue Applications of Digital Technologies in the Heritage Preservation)
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25 pages, 9580 KB  
Article
Research on Mechanical Characteristics of Portal Frame Anti-Uplift Structure
by Tingting Ma, Jun He, Guolin Gao, Zhiyun Yao, Yihang Duan, Xu Zhang and Zixian Jin
Buildings 2026, 16(1), 58; https://doi.org/10.3390/buildings16010058 - 23 Dec 2025
Viewed by 301
Abstract
The complexity of the loading mode and action mechanism is demonstrated in the portal frame anti-uplift structure. The stress evolution process of the portal frame structure during the excavation of the upper foundation pit is revealed through in situ structural stress tests and [...] Read more.
The complexity of the loading mode and action mechanism is demonstrated in the portal frame anti-uplift structure. The stress evolution process of the portal frame structure during the excavation of the upper foundation pit is revealed through in situ structural stress tests and numerical modeling analysis reflecting the small strain characteristics of stratum. The stress distribution of uplift piles and anti-floating plates is analyzed, with the axial force of piles and the development law of bending moment in plates being specifically examined. It is emphasized that the load of the uplift pile is generated by friction between the pile and soil caused by stratum floating, which is predominantly produced during the excavation of the upper block and the unloading of the surcharge. The pile 11# is observed to be under tension in the middle and compressed at both ends, with the extreme value of tensile stress of these 24 piles being located at 0.15 times the pile length below the top of the middle pile. The main loads of the anti-floating plate are identified as backfilling, foundation buoyancy, and lateral soil pressure. The lower part of the two pile spans is subjected to tension, while the upper part is under compression, with the bending moment extremes being located on the side where the frame is first formed. A significant increase in stiffness is exhibited by the frame structure after its formation, and the influence from the excavation of other blocks is markedly reduced. The most adverse condition is determined to occur during the integral removal of the upper surcharge. The reference value of these research results is confirmed for clarifying the stress mechanism of anti-uplift portal frame structures and optimizing key technical parameters in structural design and construction. Full article
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24 pages, 17013 KB  
Article
Quantitative Analysis of Life-Cycle Embodied Carbon in Residential Buildings Under Different Design Patterns
by Rihan Hai, Ruijie Du, Yu Shao, Limuge Che, Ruihong Yan, Quanyi Zheng, Yuling Chen and Mengxiao Jin
Buildings 2025, 15(24), 4477; https://doi.org/10.3390/buildings15244477 - 11 Dec 2025
Viewed by 484
Abstract
The building sector is a major source of global carbon emissions, with embodied carbon playing an increasingly critical role. This study quantitatively compares the life-cycle embodied carbon of three residential building design patterns (cast-in situ, CIS; design for disassembly, DfD; and skeleton–infill, SI) [...] Read more.
The building sector is a major source of global carbon emissions, with embodied carbon playing an increasingly critical role. This study quantitatively compares the life-cycle embodied carbon of three residential building design patterns (cast-in situ, CIS; design for disassembly, DfD; and skeleton–infill, SI) under a unified scenario of a 90-year service life with functional renovations every 30 years. A total of 36 cases, derived from twelve prototypical residential designs implemented in each pattern, were evaluated via life-cycle assessment following the EN 15978 standard. The results show that the SI pattern reduces embodied carbon by 45–55% compared to CIS, while the DfD pattern achieves a 35–45% reduction (with SI pattern consistently performing best). Structural system selection also influences outcomes, with shear wall structures reducing emissions by 7–14% compared to frame systems. Plan layout effects were marginal. The analysis indicates that the design pattern is the dominant factor influencing embodied carbon outcomes. SI pattern yields the largest carbon reduction by pairing a long-lived structural frame with flexible infill that extends service life and adaptability, while DfD lowers material demand via component reuse. These findings highlight the substantial benefits of circular design strategies and support a shift toward more adaptable, long-lifespan, and low-carbon residential design. Full article
(This article belongs to the Section Building Energy, Physics, Environment, and Systems)
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21 pages, 3854 KB  
Article
Model Updating of an Offshore Wind Turbine Support Structure Based on Modal Identification and Bayesian Inference
by Chi Yu, Jiayi Deng, Chao Chen, Mumin Rao, Congtao Luo and Xugang Hua
J. Mar. Sci. Eng. 2025, 13(12), 2354; https://doi.org/10.3390/jmse13122354 - 10 Dec 2025
Viewed by 412
Abstract
Offshore wind turbine support structures are in harsh and unsteady marine environments, and their dynamic characteristics could change gradually after long-term service. To better understand the status and improve remaining life estimation, it is essential to conduct in situ measurement and update the [...] Read more.
Offshore wind turbine support structures are in harsh and unsteady marine environments, and their dynamic characteristics could change gradually after long-term service. To better understand the status and improve remaining life estimation, it is essential to conduct in situ measurement and update the numerical models of these support structures. In this paper, the modal properties of a 5.5 MW offshore wind turbine were first identified by a widely used operational modal analysis technique, frequency-domain decomposition, given the acceleration data obtained from eight sensors located at four different heights on the tower. Then, a finite element model was created in MATLAB R2020a and a set of model parameters including scour depth, foundation stiffness, hydrodynamic added mass and damping coefficients was updated in a Bayesian inference frame. It is found that the posterior distributions of most parameters significantly differ from their prior distributions, except for the hydrodynamic added mass coefficient. The predicted natural frequencies and damping ratios with the updated parameters are close to those values identified with errors less than 2%. But relatively large differences are found when comparing some of the predicted and identified mode shape coefficients. Specifically, it is found that different combinations of the scour depth and foundation stiffness coefficient can reach very similar modal property predictions, meaning that model updating results are not unique. This research demonstrates that the Bayesian inference framework is effective in constructing a more accurate model, even when confronting the inherent challenge of non-unique parameter identifiability, as encountered with scour depth and foundation stiffness. Full article
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21 pages, 1692 KB  
Article
HPV Vaccine Uptake and Cervical Cancer Trends in Panama: A Reference Point for Future Impact Studies
by Arlene Calvo, Sabrina Hall, Verónica B. Melgar Cossich, Jonathan Andreadakis, Humberto López Castillo, Dalys Pinto and Itzel de Hewitt
Vaccines 2025, 13(11), 1173; https://doi.org/10.3390/vaccines13111173 - 19 Nov 2025
Viewed by 1020
Abstract
Background: Cervical cancer (CC) continues to be an important public health concern in Latin America, where it is the second cause of cancer-related deaths among women. With its strong culture of vaccination, Panama was the first country to implement the HPV vaccine [...] Read more.
Background: Cervical cancer (CC) continues to be an important public health concern in Latin America, where it is the second cause of cancer-related deaths among women. With its strong culture of vaccination, Panama was the first country to implement the HPV vaccine as part of its Essential Program on Immunization (EPI). Recently, the government implemented the 90:70:90 PAHO/WHO strategy to reach milestones toward CC elimination. Objective: This analysis triangulates and assesses national data on HPV vaccination coverage, screening practices, and cervical cancer incidence and mortality in Panama, to understand historical tendencies to date and establish a comprehensive foundation for future impact evaluations and research studies. The analysis aims to identify trends and gaps in prevention efforts and to serve as a reference point for future research on HPV-associated cancers. Methods: Population-based, descriptive, observational, ecological study where four, aggregate, de-identified data sources by various curators in Panama were match-merged by year, sex, and administrative division. Reported outcomes include HPV vaccine coverage, CC incidence and mortality rates, screening Pap tests, and CC behavior at diagnosis (in situ vs. invasive). Results: Panama has high HPV vaccine uptake (≥85% most years) in spite of low Pap test coverage (~10%). A decreasing trend in CC incidence has been observed continuously since the 1990s, counterintuitively to significantly increasing CC mortality rates, with most cases diagnosed as invasive and among younger women (30–69 years old). Conclusions: This report provides a comprehensive foundation for understanding trends in HPV vaccination coverage, cervical cancer incidence and mortality, and screening practices in Panama. While high vaccine uptake and declining incidence trends are encouraging, persistent low screening rates and elevated mortality—particularly at invasive stages among younger women—highlight critical gaps in prevention efforts. The need for integrated strategies that strengthen data systems, improve early detection, and address structural and sociocultural barriers are discussed, framed within Panama’s progress toward achieving the 90:70:90 targets. Future studies should focus on understanding non-medical influences on health and further vaccine impact with patient-level data, and other forms of HPV-related cancers in immunosuppressed populations. Public strategies would benefit from the implementation of real-life data and efficient data management, consolidation systems, systematic health promotion interventions, and an increase in resource allocation for women at the highest risk. Full article
(This article belongs to the Collection HPV-Vaccines)
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19 pages, 9959 KB  
Article
Viola–Jones Algorithm in a Bioindicative Holographic Experiment with Daphnia magna Population
by Victor Dyomin, Mickhail Kurkov, Vladimir Kalaida, Igor Polovtsev and Alexandra Davydova
Appl. Sci. 2025, 15(22), 12193; https://doi.org/10.3390/app152212193 - 17 Nov 2025
Viewed by 300
Abstract
This study considers the applicability and effectiveness of the Viola–Jones method to automatically distinguish zooplankton particles from the background in images reconstructed from digital holograms obtained in natural conditions. For the first time, this algorithm is applied to holographic images containing coherent noise [...] Read more.
This study considers the applicability and effectiveness of the Viola–Jones method to automatically distinguish zooplankton particles from the background in images reconstructed from digital holograms obtained in natural conditions. For the first time, this algorithm is applied to holographic images containing coherent noise and residual defocusing. The method was trained on 880 annotated (marked) holographic images of Daphnia magna along with 120 background frames. It was then tested on independent laboratory and field datasets, including morphologically related taxa. With optimized settings, the precision of the algorithm reached ~90% and F1~85% on noisy holographic images, and the algorithm also demonstrated the preliminary ability to recognize similar taxa without retraining. The algorithm is well suited for analyzing holographic data as a fast and resource-efficient pre-filter—it effectively separates particles from the background and thereby allows subsequent classification or its application in real-time aquatic environment monitoring systems. The article presents experimental results demonstrating the efficiency of this algorithm during plankton monitoring in situ. Full article
(This article belongs to the Section Marine Science and Engineering)
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22 pages, 57638 KB  
Article
Comparison of a Semiempirical Algorithm and an Artificial Neural Network for Soil Moisture Retrieval Using CYGNSS Reflectometry Data
by Hamed Izadgoshasb, Emanuele Santi, Flavio Cordari, Leila Guerriero, Leonardo Chiavini, Veronica Ambrogioni and Nazzareno Pierdicca
Remote Sens. 2025, 17(21), 3636; https://doi.org/10.3390/rs17213636 - 3 Nov 2025
Viewed by 793
Abstract
This research, carried out within the framework of the European Space Agency’s second Scout mission (HydroGNSS), seeks to utilize CYGNSS Level 1B products over land for soil moisture estimation. The approach involves a novel physically based algorithm, which inverts a semiempirical forward model [...] Read more.
This research, carried out within the framework of the European Space Agency’s second Scout mission (HydroGNSS), seeks to utilize CYGNSS Level 1B products over land for soil moisture estimation. The approach involves a novel physically based algorithm, which inverts a semiempirical forward model of surface reflectivity proposed in the literature. An Artificial Neural Network (ANN) algorithm has also been developed. Both methods are implemented in the frame of the HydroGNSS mission to make the most of the reliability of an approach rooted in a physical background and the power of a data-driven approach that may suffer from limited training data, especially right after launch. The study aims to compare the results and performance of these two methods. Additionally, it intends to evaluate the impact of auxiliary data. The static auxiliary data include topography, Above Ground Biomass (AGB), land cover, and surface roughness. Dynamic auxiliary data include Vegetation Water Content (VWC) and Vegetation Optical Depth (VOD) from Soil Moisture Active Passive (SMAP), as well as Normalized Difference Vegetation Index (NDVI) and Normalized Difference Water Index (NDWI) from Moderate Resolution Imaging Spectroradiometer (MODIS), on enhancing the accuracy of retrievals. The algorithms were trained and validated using target soil moisture values derived from SMAP L3 global daily products and in situ measurements from the International Soil Moisture Network (ISMN). In general, the ANN approach outperformed the semiempirical model with RMSE = 0.047 m3 m−3 and R = 0.91. We also introduced a global stratification framework by intersecting land cover classes with climate regimes. Results show that the ANN consistently outperforms the semiempirical model in most strata, achieving around RMSE = 0.04 m3 m−3 and correlations above 0.8. The semiempirical model, however, remained more stable in data-scarce conditions, highlighting complementary strengths for HydroGNSS. Full article
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21 pages, 927 KB  
Article
Female Public Sculptures: Visibly Invisible
by Miguel Ángel Posso-Yépez, Gandhy Leonardo Godoy-Guevara, Ángela Mikaela Posso-Astudillo and Carlos Israel Almeida-Vargas
Soc. Sci. 2025, 14(9), 516; https://doi.org/10.3390/socsci14090516 - 27 Aug 2025
Cited by 1 | Viewed by 1702
Abstract
Monuments and public sculptures embody collective memory, values, and identity. This study analyses the representation of women in public sculptures in Ibarra, Ecuador, and evaluates citizens’ recognition of the historical figures depicted. A mixed-method, cross-sectional design was employed. An urban inventory was conducted [...] Read more.
Monuments and public sculptures embody collective memory, values, and identity. This study analyses the representation of women in public sculptures in Ibarra, Ecuador, and evaluates citizens’ recognition of the historical figures depicted. A mixed-method, cross-sectional design was employed. An urban inventory was conducted (N = 124 sculptures), and questionnaires were administered in situ to 1200 adult residents using non-probability intercept sampling (100 surveys at each of the 12 female monuments). The results reveal a marked disparity: 55.6% of the sculptures represent men, compared with only 9.7% representing women. Recognition is minimal: 98.6% of respondents did not identify the person represented, and 95.1% reported no knowledge of her history. These findings suggest that the underrepresentation of women in public art reflects enduring structural and cultural gender inequalities. The limited presence of female monuments contributes to the erasure of women’s legacy from collective memory and perpetuates the perception of public space as historically male-dominated. Framed within the literature on gender and monuments as devices of social memory, the study advocates for inclusive commemorative policies and interpretive strategies. Limitations include the non-random sampling and single-city scope; future research should expand comparisons across cities and assess potential interventions. Full article
(This article belongs to the Special Issue Gender Knowledges and Cultures of Equalities in Global Contexts)
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32 pages, 2285 KB  
Article
Bridging the Construction Productivity Gap—A Hierarchical Framework for the Age of Automation, Robotics, and AI
by Michael Max Bühler, Konrad Nübel, Thorsten Jelinek, Lothar Köhler and Pia Hollenbach
Buildings 2025, 15(16), 2899; https://doi.org/10.3390/buildings15162899 - 15 Aug 2025
Cited by 2 | Viewed by 4256
Abstract
The construction sector, facing a persistent productivity gap compared to other industries, is hindered by fragmented value streams, inconsistent performance metrics, and the limited scalability of process improvements. We introduce a pioneering, four-tiered hierarchical productivity framework to respond to these challenges. This innovative [...] Read more.
The construction sector, facing a persistent productivity gap compared to other industries, is hindered by fragmented value streams, inconsistent performance metrics, and the limited scalability of process improvements. We introduce a pioneering, four-tiered hierarchical productivity framework to respond to these challenges. This innovative approach integrates operational, tactical, strategic, and normative layers. At its core, the framework applies standardised, repeatable process steps—mapped using Value Stream Mapping (VSM)—to capture key indicators such as input efficiency, output effectiveness, and First-Time Quality (FTQ). These are then aggregated through takt time compliance, schedule reliability, and workload balance to evaluate trade synchronisation and flow stability. Higher-level metrics—flow efficiency, multi-resource utilisation, and ESG-linked performance—are integrated into an Overall Productivity Index (OPI). Building on a modular production model, the proposed framework supports real-time sensing, AI-driven monitoring, and intelligent process control, as demonstrated through an empirical case study of continuous process monitoring for Kelly drilling operations. This validation illustrates how sensor-equipped machinery and machine learning algorithms can automate data capture, map observed activities to standardised process steps, and detect productivity deviations in situ. This paper contributes to a multi-scalar measurement architecture that links micro-level execution with macro-level decision-making. It provides a foundation for real-time monitoring, performance-based coordination, and data-driven innovation. The framework is applicable across modular construction, digital twins, and platform-based delivery models, offering benefits beyond specialised foundation work to all construction trades. Grounded in over a century of productivity research, the approach demonstrates how emerging technologies can deliver measurable and scalable improvements. Framing productivity as an integrative, actionable metric enables sector-wide performance gains. The framework supports construction firms, technology providers, and policymakers in advancing robust, outcome-oriented innovation strategies. Full article
(This article belongs to the Special Issue Robotics, Automation and Digitization in Construction)
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26 pages, 9003 KB  
Article
A Pilot-Scale Gasifier Freeboard Equipped with Catalytic Filter Candles for Particulate Abatement and Tar Conversion: 3D-CFD Simulations and Experimental Tests
by Alessandra Tacconi, Pier Ugo Foscolo, Sergio Rapagnà, Andrea Di Carlo and Alessandro Antonio Papa
Processes 2025, 13(7), 2233; https://doi.org/10.3390/pr13072233 - 12 Jul 2025
Cited by 1 | Viewed by 1202
Abstract
This work deals with the catalytic steam reforming of raw syngas to increase the efficiency of coupling gasification with downstream processes (such as fuel cells and catalytic chemical syntheses) by producing high-temperature, ready-to-use syngas without cooling it for cleaning and conditioning. Such a [...] Read more.
This work deals with the catalytic steam reforming of raw syngas to increase the efficiency of coupling gasification with downstream processes (such as fuel cells and catalytic chemical syntheses) by producing high-temperature, ready-to-use syngas without cooling it for cleaning and conditioning. Such a combination is considered a key point for the future exploitation of syngas produced by steam gasification of biogenic solid fuel. The design and construction of an integrated gasification and gas conditioning system were proposed approximately 20 years ago; however, they still require further in-depth study for practical applications. A 3D model of the freeboard of a pilot-scale, fluidized bed gasification plant equipped with catalytic ceramic candles was used to investigate the optimal operating conditions for in situ syngas upgrading. The global kinetic parameters for methane and tar reforming reactions were determined experimentally. A fluidized bed gasification reactor (~5 kWth) equipped with a 45 cm long segment of a fully commercial filter candle in its freeboard was used for a series of tests at different temperatures. Using a computational fluid dynamics (CFD) description, the relevant parameters for apparent kinetic equations were obtained in the frame of a first-order reaction model to describe the steam reforming of key tar species. As a further step, a CFD model of the freeboard of a 100 kWth gasification plant, equipped with six catalytic ceramic candles, was developed in ANSYS FLUENT®. The composition of the syngas input into the gasifier freeboard was obtained from experimental results based on the pilot-scale plant. Simulations showed tar catalytic conversions of 80% for toluene and 41% for naphthalene, still insufficient compared to the threshold limits required for operating solid oxide fuel cells (SOFCs). An overly low freeboard temperature level was identified as the bottleneck for enhancing gas catalytic conversions, so further simulations were performed by injecting an auxiliary stream of O2/steam (50/50 wt.%) through a series of nozzles at different heights. The best simulation results were obtained when the O2/steam stream was fed entirely at the bottom of the freeboard, achieving temperatures high enough to achieve a tar content below the safe operating conditions for SOFCs, with minimal loss of hydrogen content or LHV in the fuel gas. Full article
(This article belongs to the Section Chemical Processes and Systems)
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23 pages, 5570 KB  
Article
Evaluation of Coastal Sediment Dynamics Utilizing Natural Radionuclides and Validated In-Situ Radioanalytical Methods at Legrena Beach, Attica Region, Greece
by Christos Tsabaris, Alicia Tejera, Ronald L. Koomans, Damien Pham van Bang, Abdelkader Hammouti, Dimitra Malliouri, Vasilios Kapsimalis, Pablo Martel, Ana C. Arriola-Velásquez, Stylianos Alexakis, Effrosyni G. Androulakaki, Georgios Eleftheriou, Kennedy Kilel, Christos Maramathas, Dionisis L. Patiris and Hannah Affum
J. Mar. Sci. Eng. 2025, 13(7), 1229; https://doi.org/10.3390/jmse13071229 - 26 Jun 2025
Viewed by 1288
Abstract
This study was realized in the frame of an IAEA Coordinated Research Project for the evaluation of sediment dynamics, applying in-situ radiometric methods accompanied with a theoretical model. The in-situ methods were validated using lab-based high-resolution gamma-ray spectrometry. Sediment dynamics assessments were performed [...] Read more.
This study was realized in the frame of an IAEA Coordinated Research Project for the evaluation of sediment dynamics, applying in-situ radiometric methods accompanied with a theoretical model. The in-situ methods were validated using lab-based high-resolution gamma-ray spectrometry. Sediment dynamics assessments were performed based on the measured and mapped activity concentrations of specific 238U progenies (214Bi or 214Pb), 232Th progenies (208Tl and 228Ac), and 40K along the shoreline of the beach. The maps of the activity concentrations of natural radionuclides were produced rapidly using software tools (R language v4.5). The sediment dynamics of the studied area were also investigated through numerical simulations, applying an open source model considering land–sea interactions and meteorological conditions and the corresponding sediment processes. The assessments, which were conducted utilizing the detailed data from the natural radioactivity maps, were validated by the simulation results, since both were found to be in agreement. Generally, it was confirmed that the distribution of radionuclides reflects the selective transport processes of sediments, which are related to the corresponding processes that occur in the study area. Legrena Beach in Attica, Greece, served as a pilot area for the comparative analysis of methods and demonstration of their relevance and applicability for studying coastal processes. Full article
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14 pages, 3106 KB  
Article
Expression and Localization of a New Parvovirus-Derived Protein in the Guinea Pig
by Camila E. Osega, Fernando J. Bustos, Francisca C. Bronfman, Robert J. Gifford and Gloria Arriagada
Viruses 2025, 17(7), 893; https://doi.org/10.3390/v17070893 - 25 Jun 2025
Viewed by 938
Abstract
Endogenous viral elements (EVEs) are genomic sequences derived from viruses. Some EVEs have open reading frames (ORFs) that can express co-opted proteins in their host. Furthermore, some EVEs that are expressed as proteins have become part of cellular genes that are fusions of [...] Read more.
Endogenous viral elements (EVEs) are genomic sequences derived from viruses. Some EVEs have open reading frames (ORFs) that can express co-opted proteins in their host. Furthermore, some EVEs that are expressed as proteins have become part of cellular genes that are fusions of hosts and EVE sequences. Endogenous parvoviral elements (EPVs) are highly represented in mammalian genomes, and some of them contain ORFs and can be expressed as proteins. We have shown that an EPV containing an ORF is part of the guinea pig gene enRep-M9l. This gene is broadly transcribed in vivo, indicating that it can be translated into a protein. By generating antibodies against the enRep coding sequence of the enRep-M9l ORF, we showed that the protein enRep-M9l is expressed in vivo and in the guinea pig-derived cell line JH4. By immunofluorescence and in situ proximity ligation assays, we observed that enRep-M9l protein has a cytoplasmic localization near microtubules. The results of this study suggest that the guinea pig EPV-derived protein enRep-M9l is a microtubule-associated protein. To our knowledge, this is the second demonstration that an EPV-derived protein is expressed in vivo. Full article
(This article belongs to the Collection Parvoviridae)
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27 pages, 13836 KB  
Article
Combining Microbial Cellulose with FeSO4 and FeCl2 by Ex Situ and In Situ Methods
by Silvia Barbi, Marcello Brugnoli, Salvatore La China, Monia Montorsi and Maria Gullo
Polymers 2025, 17(13), 1743; https://doi.org/10.3390/polym17131743 - 23 Jun 2025
Cited by 2 | Viewed by 855
Abstract
Environmentally sustainable methods for producing flexible electronics, such as paper-based energy harvesters in nanogenerators, are a major objective in materials science. In this frame, the present study investigated two different Komagataeibacter sp. strains (K2G30 and K2G44), never tested as biocatalysts for the production [...] Read more.
Environmentally sustainable methods for producing flexible electronics, such as paper-based energy harvesters in nanogenerators, are a major objective in materials science. In this frame, the present study investigated two different Komagataeibacter sp. strains (K2G30 and K2G44), never tested as biocatalysts for the production of bacterial cellulose (BC) functionalized with iron particles to provide potential electrical conductivity. Two functionalization strategies (ex situ and in situ) were evaluated using two iron compounds FeCl2 and FeSO4, individually and in combination (up to 0.1% w/v), to assess efficiency and feasibility. In addition, a Design of Experiment approach was implemented to calculate quantitative mathematical models to correlate the functionalization methods with the iron amount in the BC. Among the tested conditions, BC produced by strain K2G44 using the ex situ method with FeCl2 showed the most promising results, achieving the highest iron content (~37% atomic weight) with a highly homogeneous dispersion of iron nanoparticles. Moreover, the in situ BC functionalization using FeSO4 led to the formation of iron gluconate. FeSO4 alone significantly enhanced BC production in the in situ process, with yields of 2.62 ± 0.15 g/L for K2G30 and 2.05 ± 0.09 g/L for K2G44. Full article
(This article belongs to the Special Issue Sustainable Bio-Based and Circular Polymers and Composites)
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