Search Results (413)

The incorporation of Cannabis sativa L. seeds into barley wort was investigated to enhance the functional profile of beer. Hemp seeds (cv. Henola) were malted via controlled steeping, germination, and kilning, then added to barley malt at 10% and 30% (w/w) in both malted and unmalted forms. Standard congress mashing produced worts whose physicochemical parameters (pH, extract, colour, turbidity, filtration and saccharification times) were assessed, alongside profiles of fermentable sugars, polyphenols, B-group vitamins, and cannabinoids. Addition of hemp seeds reduced extract yield without impairing saccharification or filtration and slightly elevated mash pH and turbidity. Maltose and glucose levels declined significantly at higher hemp dosages, whereas sucrose remained stable. Wort enriched with 30% unmalted seeds exhibited the highest levels of trans-ferulic (20.61 µg/g), gallic (5.66 µg/g), trans-p-coumaric (3.68 µg/g), quercetin (6.07 µg/g), and trans-cinnamic (4.07 µg/g) acids. Malted hemp addition enhanced thiamine (up to 0.302 mg/mL) and riboflavin (up to 178.8 µg/mL) concentrations. Cannabinoids (THCA-A, THCV, CBDV, CBG, CBN) were successfully extracted at µg/mL levels, with the total cannabinoid content peaking at 14.59 µg/mL in the 30% malted treatment. These findings demonstrate that hemp seeds, particularly in malted form, can enrich barley wort with bioactive polyphenols, vitamins, and non-psychoactive cannabinoids under standard mashing conditions, without compromising key brewing performance metrics. Further work on fermentation, sensory evaluation, stability, and bioavailability is warranted to realise hemp-enriched functional beers. Full article

Hemp (Cannabis sativa L.) products have gained attention in poultry nutrition for their rich content of polyunsaturated fatty acids (PUFAs), bioactive compounds, and potential functional benefits. However, findings on their impact on laying-hen performance, egg quality, and yolk fatty acid profiles have been inconsistent. This meta-analysis aimed to evaluate the effects of dietary hemp products on laying-hen performance, egg quality traits, and yolk fatty acid composition, while exploring potential sources of heterogeneity across studies. A comprehensive literature search identified 21 studies that met the inclusion criteria. A random-effects model was used to calculate standardized mean differences (SMDs) with 95% confidence intervals (CIs) for various outcomes, including production performance, egg quality, and yolk fatty acid profiles. Subgroup and meta-regression analyses assessed the influence of factors such as inclusion level, hen age, and hemp product type. The results showed that hemp supplementation had no significant effect on hen-day production, egg mass, feed conversion ratio, or feed intake. However, yolk redness (SMD = 4.40; 95% CI: 2.46, 6.33; p < 0.001) and yellowness (SMD = 4.45; 95% CI: 2.75, 6.16; p < 0.001) were significantly enhanced. Hemp feeding also increased n-3 PUFA levels in egg yolk, including C18:3n3, C20:5n3, and C22:6n3, while reducing saturated and monounsaturated fatty acids. Subgroup analysis indicated that inclusion levels > 10% and hen age ≤ 25 weeks were associated with slight reductions in hen-day production, whereas older hens (>25 weeks) showed increased egg weight. In conclusion, hemp products can enrich yolk pigmentation and n-3 PUFA content in eggs, supporting the production of functional, value-added eggs without compromising the laying-hen performance. Full article

This study presents the application of flax (Linum usitatissimum L.) and hemp (Cannabis sativa L.) fibers into composites with polyethylene matrices. The applied fibers were obtained using osmotic, water-retting, and dew-retting processes. The study determined the impact of the fiber extraction method on the properties of the composites obtained from natural filler and polyethylene matrix. These properties included color, tensile strength, thermal stability, adhesion of filler to the polymer, and flammability. It has been shown that the addition of flax and hemp fibers improves the mechanical properties of the composite compared to pure polymer. The tensile strength of the pure polymer samples was 24.64 MPa, while the tensile strength of composites reinforced with flax fibers ranged from 31.26 to 34.45 MPa, and those reinforced with hemp fibers ranged from 31.41 to 33.36 MPa. Studying the composites’ flammability showed that filling them with osmotic degummed hemp fibers reduced the maximum heat release rate by over 34% for hemp compared to pure polymer. This research shows that the composites filled with flax and hemp fibers, regardless of extraction method, are characterized by reduced flammability and improved mechanical properties compared to the pure polyethylene samples. Full article

Hemp (Cannabis sativa L.) seeds have been essential for human nutrition for millennia. The products and by-products of hemp seeds are gaining popularity nowadays as food, feed and medicine for their high nutritional and nutraceutical properties. In parallel, concerns about phytate, an antinutritional compound limiting nutrient bioavailability in hemp seeds and seed meal are rising. Hemp seeds contain an array of nutrients, but their bioavailability is mostly unknown. Here, we report nutrient and phytate concentrations and phytate contents in source seeds and multiplied seeds of seven industrial hemp varieties. We estimated the bioavailability of specific nutrients based on calculated molar ratios of phytate to minerals. Seed multiplication was carried out in a phytotron using a compost-based growth medium. Five macronutrients (P, K, Mg, S, Ca), four micronutrients (Fe, Mn, Zn, Cu) and Cr were measured in seeds using ICP-OES. Seed phytate was determined using a UV-visible spectrophotometer rapid colourimetric assay. The results revealed significant differences between seven industrial hemp varieties for most macro- and micronutrient concentrations (not Fe), phytate concentration and content and phytate-to-mineral molar ratios in both source and multiplied seeds. Multiplied hemp seeds had higher K, Mn and Zn and, lower Cr and phytate concentrations and lower phytate content than source seeds. Considering nutrient bioavailability, Ca and Fe are non-bioavailable, and Zn is bioavailable in hemp seeds. Ferimon has increased Zn bioavailability in source and multiplied seeds, indicating the variety’s potential for seed production in Western Australia. Full article

The global incidence of cancer continues to rise at an alarming rate, with annual cases projected to increase by 47% from 19.3 million in 2020 to 28.4 million by 2025. Cannabis sativa L. was among the earliest plants investigated for potential anticancer therapies, due to its more than 100 bioactive constituents that confer notable antioxidant properties. Hemp-derived extracts, particularly those rich in cannabidiol (CBD), exhibit notable synergistic biological effects, including the inhibition of cancer cell proliferation, angiogenesis, and metastasis, alongside the promotion of apoptosis. These pharmacological attributes suggest that hemp oils may serve as promising alternatives or adjuncts to conventional chemotherapy, offering potential therapeutic benefits with a reduced risk of severe adverse effects. This review discusses the current literature on hemp oils, with emphasis on their roles in cancer prevention, therapeutic efficacy, and potential toxicity in humans. Furthermore, it explores the various extraction methods employed in hemp oil production and examines their chemical compositions, offering a comprehensive understanding of the principal antioxidant constituents responsible for their bioactivity to the readers. Full article

Cannabis (Cannabis sativa L.) contains numerous secondary metabolites with different bioactivities. Extraction methods differ in their efficiency in recovering metabolites from plant material, and thus cannabis extracts vary significantly in their composition and activity. We aimed to develop a repeatable and accurate HPLC-MS method for the determination of nine common cannabinoids and compare two widely used extraction techniques: ultrasound-assisted extraction (UAE) with methanol and supercritical CO₂ extraction (SFE). Inflorescences of the Kompolti cultivar were used as the plant material. On a polar C18 column, more than thirty compounds were well separated within 25 min; thirteen cannabinoids were identified and eight of them were quantified, with cannabidiol and its acidic precursor being the most abundant. Additionally, three spectrophotometric assays were employed for extract characterization: the total phenolic content, total flavonoid content, and DPPH radical scavenging capacity. The SFE extract, obtained using ethanol as a co-solvent under low pressure (<100 bar) and temperature (<45 °C), was more enriched than the UAE extract (181.62 ± 2.90 vs. 140.64 ± 13.24 mg quercetin equivalents/g of dry extract) and cannabinoids (446.29 ± 22.66 vs. 379.85 ± 17.16 mg/g of dry extract), especially cannabinoid acids. However, UAE achieved greater recovery from the plant material (cannabinoids: 83.42 ± 5.15 vs. 68.84 ± 3.49 mg/g of plant material) and showed superior antioxidant capacity (DPPH IC₅₀: 2.50 ± 0.18 vs. 3.37 ± 0.07 mg/mL). Notwithstanding the observed partial decarboxylation, the high repeatability (RSD < 15%, n = 11) of the entire analytical workflow involving UAE extraction and LC-MS analysis renders it suitable for routine analyses. This study contributes to the ongoing efforts toward the quality control and valorization of C. sativa. Full article

Industrial hemp (Cannabis sativa L.) is gaining renewed interest as a sustainable source of natural fibres, particularly in regions like Belgium, where well-established flax processing infrastructure exists. However, region-specific data on varietal performance for long fibre production remain limited, hindering large scale adoption by both farmers and processors. This study aimed to assess the agronomic performance of early- and late-flowering hemp varieties under temperate maritime conditions through a three-year field trial at two sites in Flanders (Belgium). The evaluation focused on key parameters including emergence rate, plant morphology, long fibre yield and quality. Results showed that successful crop establishment is critical, as poor emergence influenced stem diameter (increasing with decreasing emergence) and leads to reduced long fibre yields by up to 50% between tested sites. Significant yield differences between trial years were present at both sites, with the biomass yield ranging from 10.7 to 14.5 and from 7.8 to 9.6 t ha⁻¹ for Bottelare and Beitem, respectively. Under favourable conditions, long fibre yields reached up to 2.4 t ha⁻¹ for late-flowering and 2.1 t ha⁻¹ for early- and mid-late-flowering varieties. In Western Europe, early to mid-late flowering varieties are generally favoured. Among these, the mid-late flowering variety Bialobrzeskie demonstrated strong yield potential. Overall, fibre quality across varieties was comparable to that of flax. Full article

Cannabis sativa L. is known for its high-value compounds, like Cannabidiol (CBD) and Cannabidiolic Acid (CBDA). It is widely used in the pharmaceutical and food industries. Different extraction methods, like Soxhlet and maceration, are commonly employed to obtain its extracts. High temperature and long extraction time can influence the yield and the purity of the extracts, affecting the quality of the final product. This study focused on optimizing CBD oil extraction from hemp inflorescences and its incorporation into a gluten-free bakery product for functionalization. Dynamic maceration (DME), assisted by ultrasound and microwave irradiation, was used. Our study explored the impact of varying sonication times (three distinct durations) and microwave powers (three levels, applied for two different irradiation times) on the resulting extracts. HPLC analysis was performed on these extracts. Subsequently, we used hemp flour and hemp oil to bake gluten-free cupcakes, which were fortified with the extracted CBD oil. Rheological characterization was used to investigate the cupcake properties, along with stereoscopic, color and puncture analysis performed on the baked samples. The most effective extraction parameters identified were 30 s of microwave irradiation at 700 W, yielding 45.2 ± 2.0 g of CBD extract, and 15 min of sonication, which resulted in 53.2 ± 2.5 g. Subsequent rheological characterization indicated that the product exhibited mechanical properties and a temperature profile comparable to a benchmark, evidenced by a height of 4.1 ± 0.2 cm and a hardness of 1.9 ± 0.2 N. These promising values demonstrate that hemp oil and hemp flour are viable ingredients for traditional cakes and desserts, notably contributing increased nutritional value through the CBD-enriched hemp oil and the beneficial profile of hemp flour. Full article

This study investigates the environmental regulation of sex expression and seed yield stability in four Thai dioecious hemp (Cannabis sativa L.) cultivars (RPF1, RPF2, RPF3, and RPF4) under different seasonal conditions to optimize seed production. The experiment was conducted across two planting periods (in-season and off-season) to evaluate the effects of varying day lengths and temperatures on growth, reproductive development, and yield. The results showed that shorter day lengths and lower temperatures during the off-season led to an increased proportion of female plants across all cultivars, except RPF3, which exhibited a stable female-to-male ratio. RPF4 had the highest increase in female plants (16%), followed by RPF1 and RPF2 (10%). Seed yield was significantly influenced by seasonal changes, with RPF3 and RPF4 consistently outperforming the other cultivars. In the in-season, RPF3 and RPF4 produced the highest seed yields, reaching 83.4 g/plant and 81.6 g/plant, respectively. During the off-season, both cultivars experienced a decline in seed yield (by 24–26%), primarily due to a reduction in seed production in secondary inflorescences. However, RPF3 compensated for this loss with a 31% increase in seed production at main inflorescences, ensuring yield stability. RPF4 maintained its high yield potential by increasing the proportion of female plants, offsetting the decline in seed yield per plant. Additionally, cumulative growing degree days (CGDD) at harvest were comparable between seasons, with values of 2434 °Cd (in-season) and 2502 °Cd (off-season), indicating that temperature accumulation remained within an optimal range for seed maturation. The study highlights the importance of cultivar selection based on yield component stability and adaptability to seasonal variations. These findings provide valuable insights for improving hemp seed production strategies in Thailand’s diverse agro-climatic conditions. Full article

Hemp (Cannabis sativa L.) is a versatile crop that can be processed to obtain different products with multiple applications. Its seeds are a well-documented ancient source of proteins, fibers and fats, all of which possess high nutritional value. Additionally, metabolites such as flavones and phenols are present in the seeds, contributing to their antioxidant properties. Due to hemp seeds’ distinctive nutritional profile, the interest in exploring the potential use in food and nutraceuticals is growing, and they can be considered an interesting and promising alternative resource for human and animal feeding. Omics studies on hemp seeds and their by-products are also being developed, and they contribute to improving our knowledge about the genome, transcriptome, proteome, metabolome/lipidome, and ionome of these sustainable food resources. This review illustrates the main nutrients and bioactive compounds of hemp seeds and explores the most relevant omics techniques and investigations related to them. It also addresses the various products derived from processing the whole seed, such as oil, dehulled seeds, hulls, flour, cakes, meals, and proteins. Moreover, this work discusses research aimed at elucidating the molecular mechanisms underlying their protein, lipid, fiber, and metabolic profile. The advantages of using omics and multi-omics approaches to highlight the nutritional values of hemp seed by-products are also discussed. In our opinion, this work represents an excellent starting point for researchers interested in studying hemp seeds as source of nutrients and bioactive compounds from a multi-level molecular perspective. Full article

Optimizing nutrient usage and controlling environmental parameters are crucial for improved crop growth and yield in the cultivation of Cannabis sativa, commonly known as hemp, using controlled-environment agriculture (CEA) systems. Although hemp cultivation in CEA is rapidly growing, the effects of different light-intensity treatments on early vegetative stages of hemp grown in hydroponic and aquaponic systems, along with the impacts on the environment and human health remains limited. This study employed a split-plot design, consisting of two layers of plant grow beds where each layer was exposed to different light intensities (LIs): approximately 752 µmol/m²/s (high light intensity) on the upper layer and 141 µmol/m²/s (low light intensity) on the lower layer. To assess the influence of two different LIs on plant growth indicators, specifically plant length and leaf area, the environmental parameters, including dissolved oxygen (DO), electrical conductivity (EC), pH, and water temperature (WT) were maintained within the same range for both systems. Additionally, the study incorporated a cradle-to-gate life cycle assessment (LCA) to precisely evaluate the environmental performance of both systems. Under the specific environmental and design conditions of this study, hemp plants grown in aquaponics showed greater growth performance in plant length compared to hydroponics (more than 42% higher for both LIs) and leaf area (28.3% greater under 141 µmol/m²/s), although the leaf area was 2.1% lower under 752 µmol/m²/s compared to plants grown in hydroponics. The LCA demonstrated that the aquaponic system provided an efficient and sustainable approach by integrating fish with hemp cultivation. The LCA results showed that aquaponics had a 22% reduction in midpoint and a 15% reduction in endpoint impact in contrast to the hydroponics system for hemp leaf cultivation. This research highlights the potential of aquaponic systems as a viable and sustainable alternative to hydroponic systems for hemp leaf cultivation in CEA under uncertain future climates. Full article

The feasibility of using a combination of organic fertilizer with a reduced rate of chemical nitrogen fertilizer as an alternative to conventional inorganic fertilization was tested on the growth and biomass accumulation of hemp plants and the phytochemical accumulation in their inflorescences. To achieve this goal, a field experiment was set up with the following nine treatments: F0, no fertilizer; NPK, mineral fertilizer with 100 kg ha⁻¹ nitrogen; C1, compost from solid digestate (50%) + cardoon-based spent mushroom substrate (50%); C2, compost from solid digestate (50%) + straw-based spent mushroom substrate (50%); C3, C4, C5, and C6, composts from solid digestate (50%, 67%, 75%, and 84%, respectively) and cardoon waste (50%, 33%, 25%, and 16%, respectively); SD, non-composted solid digestate. C1–C6 and SD were added to the soil, along with half the rate (50 kg ha⁻¹) of chemical nitrogen fertilizer. Taking F0 as a reference, all fertilized treatments, except C6 and SD, showed a notable increase in plant growth and biomass accumulation in the stem, inflorescence, and whole plant. Among the organic treatments, the best growth performances were detected in C1 and C5, which reached, or even exceeded, that of NPK. Compared to F0, all fertilized treatments had high phenolic acid and flavonoid yields, while high carotenoid, tocopherol, terpene, and cannabinoid (mainly CBD) yields were detected in all fertilized treatments except C6 and SD. Among the organic treatments, C1 and C5 stood out for their highest phenolic acid, flavonoid, carotenoid, and tocopherol yields, while C1, C2, and C3 stood out for their highest terpene and cannabinoid yields, which, in both cases, reached, or even exceeded, those of NPK. Overall, our findings show that 50% replacement of inorganic nitrogen fertilizer with C1 to C5 composts may represent a cost-effective and environmentally safe alternative to conventional inorganic fertilization that can sustain the growth of hemp plant and the phytochemical accumulation in its inflorescences, thus promoting the use of this crop for fiber and bioenergy production, as well as for applications in food, nutraceutical, agrochemical, and cosmetic sectors. Full article

This study was aimed at providing athletes a solution to replenish the muscle glycogen re-synthesis at an optimal rate with hemp seeds as a natural protein source and Bengal gram dal and its use for the preparation of gel. The gel contains the richest source of energy, and it is an effective way to provide energy and nutrients to athletes. The gel was prepared in three variations with different hemp seed concentrations. We then analyzed the gel for pH and macronutrient composition. The sensory characteristics were analyzed for seven parameters, including appearance, taste, color, texture, aroma, consistency, and acceptability, using a hedonic scale on 25 panelists. A sensory analysis showed that sample A received an overall acceptability score of 7.16 ± 0.99 from the sensory panel. The shelf life was observed at the recommended temperature of 4 degrees Celsius, which was 12 days. The best formulation was sample B with 38 g of hemp seeds, which showed better taste, color, aroma, and acceptability and a lower average pH value (6.68 ± 1.44, 6.56 ± 1.29, 7.6 ± 1.16, 7 ± 1.26, and 5.822 ± 0.0183, respectively). Sample B contained 30.8 g of protein, 16.09 g of carbohydrates, 8.4 g of fat, and 263.16 kcal of energy per 100 g. The resulting ratio of carbohydrates to protein is optimal for use as a high-protein post-workout meal. Hence, it can be considered a post-workout supplement. Full article

This study investigated the nutritional composition, antinutritional factors, oxidative stability, microbiological safety, and sensory characteristics of hempseed cake (HC) derived from Cannabis sativa L. cv. ‘Henola’. The effects of dehulling and storage (1, 3, and 6 months) on dehulled (DHC) and hulled (HHC) hemp cake were systematically assessed. DHC exhibited significantly higher crude protein (up to 42.2%) and residual oil content (up to 37.5%), while HHC was richer in dietary fiber (up to 41.3%) and total carbohydrates (up to 48.2%). Despite comparable PUFA contents (63–72%) and favorable n-6/n-3 ratios (~3.1:1), DHC showed greater energy concentration and reduced levels of indigestible carbohydrates and phytates. Oxidative stability tests revealed increased acid and peroxide values in both HHC and DHC after six months, indicating quality deterioration (Totox index > 15). Microbiological analyses confirmed hygienic safety across all samples, with slightly higher microbial counts in HHC linked to hull-associated contamination. Sensory evaluations revealed stable color, odor, and texture during storage, with DHC rated more aromatic. These findings confirm that processing conditions—particularly dehulling—strongly affect the functional and nutritional profile of hempseed by-products. DHC emerges as a promising, shelf-stable, protein-rich ingredient for functional food and feed applications. Full article

The use of crop residues is increasing across farming systems as part of climate change mitigation efforts and agricultural management practices to improve soil health. Hemp residues offer valuable potential in these efforts due to their rich nutrient composition. However, the complex chemical composition of hemp residue could pose a significant challenge by slowing the decomposition rate if not adequately managed. The aim of this study is to evaluate the influence of different timings of hemp residue incorporation, soil tillage practices, and mode of application on the rate of mineralization and soil chemical parameters. A complete randomized design field trial was conducted on hemp (Cannabis sativa L.) residue incorporation across different seasonal periods and modes of application. The results showed that the fastest mineralization occurred when hemp residue was incorporated in autumn, while the slowest mineralization was observed when the residue was left on the surface of the soil as mulch. The application of hemp residues over three years led to a slight increase in soil pH from an initial value of 4.9; however, this change was not statistically significant. Similarly, nitrogen content did not change significantly between the different periods after applying hemp residues. In contrast, hemp residues contributed to an increase in soil carbon content. Overall, this study emphasizes the need to optimize hemp residue management to maximize its benefits for enhancing soil chemical properties and promoting sustainable agriculture. Full article