New Analytical Methods in Plant Active Components Analysis

Topic Information

Dear Colleagues,

Plants are well known as one of the greatest resources of active components that are usually used for the development of new drugs, functional foods, and dietary supplements. The discovery of new biologically active compounds from natural sources seems to be the main objective of many pharmaceuticals and food companies. Current research trends clearly indicate that natural active components will be among the most significant sources of new drugs in the future. Due to the complexity of plant active chemical constituents, the development of new robust, efficient, sensitive, and cost-effective methods to improve separation, extraction, and purification of these compounds is of great interest, which may provide the necessary basis for further study of the bioactivity, structure–activity relationships, metabolisms in vivo, structural modification, and synthesis of active compounds. This topic, “New Analytical Methods in Plant Active Component Analysis”, covers a selection of research articles, communications, or reviews that explore plant active compounds’ isolation and structural determination via analytical methods, including chromatography, mass spectrometry, spectroscopy, and electroanalysis. We are particularly interested in articles describing new analytical strategies for determining active compounds in plants, with selected applications linked to current trends in food chemistry, the environment, biology, and pharmaceuticals. Novel applications and the optimization of existing analytical methods are also welcome.

Dr. Filomena Lelario
Dr. Giuliana Bianco
Prof. Dr. Radosław Kowalski
Topic Editors

Keywords

Participating Journals

Journal Name	Impact Factor	CiteScore	Launched Year	First Decision (median)	APC
Analytica analytica	-	1.8	2020	12.8 Days	CHF 1000	Submit
Antioxidants antioxidants	6.0	10.6	2012	15.5 Days	CHF 2900	Submit
Applied Sciences applsci	2.5	5.3	2011	17.8 Days	CHF 2400	Submit
Molecules molecules	4.2	7.4	1996	15.1 Days	CHF 2700	Submit
Separations separations	2.5	3.0	2014	12.4 Days	CHF 2600	Submit
Chemosensors chemosensors	3.7	5.0	2013	17.1 Days	CHF 2700	Submit

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Published Papers (4 papers)

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All Analytica Antioxidants Applied Sciences Molecules Separations Chemosensors

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17 pages, 1936 KiB  

Open AccessArticle

Vicia faba L. Pod Valves: A By-Product with High Potential as an Adjuvant in the Treatment of Parkinson’s Disease

by Carmen Tesoro, Filomena Lelario, Fabiana Piscitelli, Angela Di Capua, Paolo Della Sala, Paola Montoro, Giuliana Bianco, Maria Assunta Acquavia, Mario Dell’Agli, Stefano Piazza and Rosanna Ciriello

Molecules 2024, 29(16), 3943; https://doi.org/10.3390/molecules29163943 - 21 Aug 2024

Viewed by 613

Abstract

Vicia faba L. is a leguminous plant with seeds rich in nutritional compounds, such as polyphenols and L-dopa, a dopamine precursor and first-line treatment for Parkinson’s symptoms. Recently, its by-products have been revalued as a sustainable source of bioactive compounds. In this study, [...] Read more.

Vicia faba L. is a leguminous plant with seeds rich in nutritional compounds, such as polyphenols and L-dopa, a dopamine precursor and first-line treatment for Parkinson’s symptoms. Recently, its by-products have been revalued as a sustainable source of bioactive compounds. In this study, aqueous extracts of Lucan broad bean pod valves (BPs) were characterized to evaluate their potential use as adjuvants in severe Parkinson’s disease. L-dopa content, quantified by LC-UV, was much higher in BPs than in seeds (28.65 mg/g dw compared to 0.76 mg/g dw). In addition, vicine and convicine, the metabolites responsible for favism, were not detected in pods. LC-ESI/LTQ-Orbitrap/MS² allowed the identification of the major polyphenolic compounds, including quercetin and catechin equivalents, that could ensure neuroprotection in Parkinson’s disease. ESI(±)-FT-ICR MS was used to build 2D van Krevelen diagrams; polyphenolic compounds and carbohydrates were the most representative classes. The neuroprotective activity of the extracts after MPP⁺-induced neurotoxicity in SH-SY5Y cells was also investigated. BP extracts were more effective than synthetic L-dopa, even at concentrations up to 100 µg/mL, due to the occurrence of antioxidants able to prevent oxidative stress. The stability and antioxidant component of the extracts were then emphasized by using naturally acidic solutions of Punica granatum L., Ribes rubrum L., and gooseberry (Phyllanthus emblica L.) as extraction solvents. Full article

(This article belongs to the Topic New Analytical Methods in Plant Active Components Analysis)

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16 pages, 2193 KiB  

Open AccessArticle

An Amperometric Biosensor Based on a Bilayer of Electrodeposited Graphene Oxide and Co-Crosslinked Tyrosinase for L-Dopa Detection in Untreated Human Plasma

by Giuseppa Cembalo, Rosanna Ciriello, Carmen Tesoro, Antonio Guerrieri, Giuliana Bianco, Filomena Lelario, Maria Assunta Acquavia and Angela Di Capua

Molecules 2023, 28(13), 5239; https://doi.org/10.3390/molecules28135239 - 6 Jul 2023

Cited by 4 | Viewed by 1485

Abstract

L-Dopa, a bioactive compound naturally occurring in some Leguminosae plants, is the most effective symptomatic drug treatment for Parkinson’s disease. During disease progression, fluctuations in L-DOPA plasma levels occur, causing motor complications. Sensing devices capable of rapidly monitoring drug levels would allow adjusting [...] Read more.

L-Dopa, a bioactive compound naturally occurring in some Leguminosae plants, is the most effective symptomatic drug treatment for Parkinson’s disease. During disease progression, fluctuations in L-DOPA plasma levels occur, causing motor complications. Sensing devices capable of rapidly monitoring drug levels would allow adjusting L-Dopa dosing, improving therapeutic outcomes. A novel amperometric biosensor for L-Dopa detection is described, based on tyrosinase co-crosslinked onto a graphene oxide layer produced through electrodeposition. Careful optimization of the enzyme immobilization procedure permitted to improve the long-term stability while substantially shortening and simplifying the biosensor fabrication. The effectiveness of the immobilization protocol combined with the enhanced performances of electrodeposited graphene oxide allowed to achieve high sensitivity, wide linear range, and a detection limit of 0.84 μM, suitable for L-Dopa detection within its therapeutic window. Interference from endogenous compounds, tested at concentrations levels typically found in drug-treated patients, was not significant. Ascorbic acid exhibited a tyrosinase inhibitory behavior and was therefore rejected from the enzymatic layer by casting an outer Nafion membrane. The proposed device was applied for L-Dopa detection in human plasma, showing good recoveries. Full article

(This article belongs to the Topic New Analytical Methods in Plant Active Components Analysis)

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12 pages, 1357 KiB  

Open AccessArticle

An Interplay between a Face-Centred Composite Experimental Design and Solid-Phase Microextraction for Wine Aroma GC/MS Analysis

by Carmen Tesoro, Maria Assunta Acquavia, Barbara Giussani, Giuliana Bianco, Raffaella Pascale, Filomena Lelario, Rosanna Ciriello, Angela Capece, Rocchina Pietrafesa, Gabriella Siesto and Angela Di Capua

Appl. Sci. 2023, 13(7), 4609; https://doi.org/10.3390/app13074609 - 5 Apr 2023

Viewed by 1354

Abstract

For oenological products, most of the intrinsic and extrinsic drivers of perceived quality are associated with specific aromatic profiles. Aromatic diversity has been recognized as a central element in perceived quality as it is able to transmit the complex interactions between grape variety, [...] Read more.

For oenological products, most of the intrinsic and extrinsic drivers of perceived quality are associated with specific aromatic profiles. Aromatic diversity has been recognized as a central element in perceived quality as it is able to transmit the complex interactions between grape variety, geographical characteristics, and viticultural and winemaking practices, including the fermentative process. A comprehensive characterization of flavour compounds by headspace solid-phase microextraction (HS-SPME) and gas chromatography coupled to mass spectrometric analysis is often needed in order to ascertain the quality of wine. HS-SPME requires a proper optimization that can be achieved through an adequate experimental design. Here, a HS-SPME/GC-MS based method was developed to investigate the volatile compounds of wine samples obtained by laboratory-scale fermentations. This was performed by inoculating a commercial Saccharomyces cerevisiae strain, which is used both as single starter and as mixed starter, with an indigenous Hanseniaspora osmophila strain. The experimental conditions of HS-SPME (extraction temperature and time) were optimized by applying a face-centred composite experimental design. Up to 95% of the total variance was explained by the proposed model. The optimized method allowed us to confirm the usefulness of combining the inoculation of grapes with selected yeast strains in co-culture situations in order to improve the wine bouquet. Full article

(This article belongs to the Topic New Analytical Methods in Plant Active Components Analysis)

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9 pages, 653 KiB  

Open AccessArticle

Simple and Rapid High-Performance Liquid Chromatography Method for Simultaneous Determination of Picloram and 2,4-D in Pesticide Formulations

by Angela Santilio, Silvana Girolimetti and Valentina Picardo

Analytica 2022, 3(4), 430-438; https://doi.org/10.3390/analytica3040029 - 20 Nov 2022

Cited by 1 | Viewed by 2653

Abstract

Picloram and 2,4-D are systemic herbicides used to control a wide range of broad-leaved weeds and post-emergence annual and perennial broad-leaved weeds, respectively. The use of plant protection products containing Picloram and 2,4-D necessitates the development of analytical methods, useful for the laboratories [...] Read more.

Picloram and 2,4-D are systemic herbicides used to control a wide range of broad-leaved weeds and post-emergence annual and perennial broad-leaved weeds, respectively. The use of plant protection products containing Picloram and 2,4-D necessitates the development of analytical methods, useful for the laboratories focused on control, for monitoring. In this study, we designed and validated an analytical method for the rapid determination of picloram and 2,4-D by HPLC-DAD. The method involves the extraction of the substances by sonication of the sample with methanol, followed by dilution in acetonitrile, and direct injection on a liquid chromatography system, based on the use of a Gemini C18 column. We used an isocratic mobile elution consisting of acetonitrile and water acidified 1% with H₃PO₄ (50:50, v/v). We validated the proposed method, which demonstrated linearity within the concentration range of 0.01–0.028 mg/mL for picloram and 2,4-D, with a correlation coefficient (R²) of 0.9993 for picloram and 0.9999 for 2,4-D. We considered precision, repeatability and selectivity in the validation. The repeatability of the method expressed as percent of relative standard deviation (%RSD) was lower than 1% for both substances. The proposed method is suitable for the simultaneous determination of picloram and 2,4-D in pesticide formulations. Full article

(This article belongs to the Topic New Analytical Methods in Plant Active Components Analysis)

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