Search Results (54)

The aroma of wine is a defining quality attribute, determined mainly by volatile organic compounds (VOCs) originating from grape metabolism, fermentation, and maturation. This study aimed to characterize the VOC composition of young monovarietal red wines from the Alentejo region (Portugal), produced from Alicante Bouschet, Touriga Nacional, and Trincadeira across five consecutive vintages (2020–2024). Headspace solid-phase microextraction (HS-SPME) coupled with comprehensive two-dimensional gas chromatography–time-of-flight mass spectrometry (GC×GC-ToFMS) was applied for VOC profiling, followed by multivariate statistical analyses. A strict identification and reproducibility criterion was applied to ensure longitudinal consistency over the five vintages. MANOVA analysis revealed significant effects (p < 0.001) of both grape variety and vintage on VOC distribution. Esters were the most abundant and discriminant group, while aldehydes and terpenes contributed markedly to varietal differentiation. Alicante Bouschet wines were associated with fruity ethyl esters, Touriga Nacional with monoterpenes (citronellol, terpinolene, α-farnesene) and aromatic alcohols, and Trincadeira with aldehydes and sesquiterpenes. Canonical discriminant analysis (CDA) achieved clear separation among varieties and vintages, with the first two canonical functions accounting for over 70% of the total variance. Heatmap analysis highlighted distinctive terpene and C13-norisoprenoid profiles across samples. These findings demonstrate the pivotal role of VOCs in defining Alentejo wine typicity and support their use as chemical markers for authenticity and PDO valorization. Full article

Wine is the world’s leading alcoholic beverage, with Greece having a centuries-old history of winemaking processes. A common practice among winemakers is the addition of herbs and plants to wine, which is believed to increase its antioxidant capacity. A well-known example is retsina, produced from resin. This paper is based on the study of Roditis Alepou (Roditis Fox) and Muscat of Patras, two euphemistic grape varieties of Achaia, at different stages of maturity, with the addition of ginger to prepare ginger-enriched wine. Ginger is considered one of the most ancient plants, with its main cultivation countries being India and Malaysia. The present study included physicochemical analyses, involving total and effective acidity of the samples, antioxidant capacity, total phenolics, and pigments in the spectra of 420, 520, and 620 nm. This work aimed to study the wine prepared by these indigenous grape varieties of Achaia with added ginger at different concentrations (0.2%, 0.5%, and 1.2%, w/v) post-fermentation, and to determine if it conforms with the typical physicochemical characteristics of dry white wine. An increase in total and effective acidity of the samples was observed. Some fluctuations in total phenolics and antioxidant capacity were noted. Finally, pigments showed increased values in all three spectra. The organoleptic evaluation yielded encouraging results, leading us to conclude that the product warrants further study, with prospects for producing wine aromatized with ginger or ginger extract. Full article

Winemaking is facing significant challenges caused by industrialization of the process, climate change, and increased consumer awareness regarding the use of chemical preservatives. Although several solutions have been proposed, the utilization of non-Saccharomyces species seems to be the most efficient one. Several non-Saccharomyces species have been employed for this purpose, with Hanseniaspora uvarum, H. vineae, Kluyveromyces marxianus, Lachancea thermotolerans, Metschnikowia pulcherrima, Pichia fermentans, P. kluyveri, Schizosaccharomyces pombe, Starmerella bacillaris, Torulaspora delbrueckii, and Wickerhamomyces anomalus being the most promising ones. However, only a restricted amount of metabolic activities can be reliably attributed to the species level, while most of them are characterized by strain variability and are also affected by the Saccharomyces cerevisiae strains used to carry out alcoholic fermentation, as well as the efficient supply of precursor molecules by the grape varieties and the conditions for their effective bioconversion. This variability necessitates the application of optimization strategies, taking into consideration all these parameters. This review article aims to assist in this direction by collecting the data referring to the winemaking practice of the most interesting non-Saccharomyces species, presenting clearly and comprehensively their most relevant features, and highlighting the effect of strain diversity. Full article

This study explores the chemical and sensory differentiation of Greek white wines produced from five indigenous grape varieties—Savvatiano, Vidiano, Moschofilero, Assyrtiko, and Malagouzia—across diverse terroirs in Greece. A targeted analytical approach was employed to quantify 12 key volatile aroma compounds derived primarily from amino acid metabolism and lipid degradation, using GC-MS and GC-FID. The selected volatiles, including isoamyl alcohol, phenylethyl alcohol, tyrosol, and hexanoic acid ethyl ester, were chosen for their sensory relevance and their biosynthetic linkage to nitrogenous precursors. Principal Component Analysis (PCA) of wines from the 2019 and 2020 vintages revealed clear varietal clustering, under standardized winemaking conditions. Malagouzia wines were characterized by rich and diverse volatile profiles, particularly long-chain fatty acids and esters, while Vidiano exhibited a consistently restrained aromatic expression. Sensory analysis using sorting and ultra-flash profiling confirmed the chemical clustering, with Moschofilero, Vidiano and Malagouzia wines forming distinct sensory groups. The findings demonstrate that key amino acid-derived volatiles can serve as biochemical markers of varietal typicity and support the use of volatile profiling as a tool for terroir-driven wine classification and quality assessment in Greek white wines. Full article

Volatile compounds and aroma sensory profile of wines were researched in order to determine the effect of the coupage technique using monovarietal young white wines from La Mancha region. To conduct this study, the aroma compositions of Airén, Chardonnay, and Vermentino monovarietal wines and the wines obtained by blending monovarietal wines were extensively studied. Free aroma compounds were isolated by solid phase extraction (SPE) using dichloromethane and then analyzed by gas chromatography–mass spectrometry (GC-MS). Sensory aroma profile was determined using Quantitative Descriptive Sensory Analysis (QDA). Seventy-one (71) free aroma compounds were identified and quantified. C₆ and benzenic compounds were the principal groups of varietal aroma compounds in Airén and Chardonnay wines. The varietal aroma of monovarietal Vermentino wines is characterized by higher concentrations of terpene and C₁₃-norisoprenoids compounds. Wines obtained by the blending of monovarietal wines showed a higher concentration of principal aroma compounds of monovarietal wines and exhibited a higher sensory complexity than the monovarietal wines. The coupage technique intensified the principal sensory properties of Airén, Chardonnay, and Vermentino monovarietal wines and these were scored very positively by wine assessors, especially when the different grape variety contents of the blended monovarietal wines were split equally at 33% each, which provided wines with more aroma typicity than monovarietal wines. Full article

Agresto is the unfermented juice traditionally obtained from boiled unripe grapes, typically using fruit that would otherwise be discarded, and enriched with spices, herbs, and fruit. In this study, the phenolic profile, antioxidant and antibacterial activity, and volatile organic compounds (VOCs) of Agresto produced from two grape varieties (Sangiovese, and Vermentino) harvested in Mount Amiata (Tuscany) were evaluated. Agresto from Vermentino showed a higher total phenolic content (TPC), 1.31 mg GAE/mL, as well as a greater total flavonoid and flavonol content and FRAP activity compared to Agresto from Sangiovese. The highest ORAC value was observed in Agresto from Vermentino, 41.01 mg TE/mL, compared to that from Sangiovese. TPC, flavonols, apocarotenes, sulfur derivatives, and non-terpene derivatives were positively correlated with antimicrobial activity against E. coli, FRAP, and ORAC. Overall, our results showed that grape variety significantly influences the chemical composition of Agresto, particularly in terms of both VOCs and phenolic compounds. The observed variations in phenolic composition also affected the antioxidant and antimicrobial activity of Agresto. These experimental findings clearly suggest the utmost importance of identifying the optimal chemical profile of “unripe grapes” used as raw material for Agresto production, considering both variety and the specific ripening degree achievable through vine green harvesting. Full article

Amino acid composition and nitrogen quantification in grape must are of great importance given their usefulness for varietal characterization, influence on fermentation processes and identification of the aromatic potential of musts. The objective of this work was to determinate the amino acid and nitrogen compound profiles of 30 minority varieties of potential use in winemaking, all grown in the same ampelographic collection in Alcalá de Henares. The concentrations of 31 amino acids in must were identified and quantified using high-performance liquid chromatography (HPLC) during four seasons (2020 to 2023), and the average values of total free amino acids (TAN), yeast assimilable nitrogen (YAN) and aromatic precursor nitrogen (APN) were calculated for each variety. ‘Tazazonal’, a minority red grape variety, was found to exhibit high concentrations of yeast assimilable nitrogen (YAN), total amino nitrogen (TAN) and ammonia nitrogen (APN), comparable to those observed in ‘Tempranillo’ and ‘Garnacha Tinta’. These two cultivars are representative of traditional Spanish red grapevine varieties and are among the most widely cultivated in the country. In the case of white varieties, ‘Albillo del Pozo’, ‘Pintada’ and ‘Verdejo Serrano’ showed higher concentrations of these parameters than ‘Malvar’ and ‘Airén’, which are also widely grown in Spain. The results revealed distinct amino acid profiles for each variety, enabling their classification and supporting the identification of variants within the autochthonous germplasm. This approach aimed to highlight minority varieties of potential interest for future studies, given their relevance to both regional and national viticulture. Full article

Greek wines made from the indigenous grape varieties Assyrtiko, Malagousia, Moschofilero and Roditis are attracting the interest of wine producers and consumers due to their aromatic characteristics. However, there are few studies that focus on the unique wine characteristics of each variety and the relationship between the composition of volatile compounds and sensory properties. Monovarietal white wines (2018 vintage) were analyzed by gas chromatography–mass spectrometry to quantify 34 volatile compounds. Multivariate statistical analyses were used to investigate correlations between volatiles and sensory attributes identified by a trained panel. The results showed that the strongest aroma compounds were a group of terpenes, isoamyl acetate and phenylethyl acetate. Terpenes such as geraniol, α-terpineol, linalool and cis-rose oxide correlated with floral notes, especially in Moschofilero wines. In addition, isoamyl acetate contributed to the aroma of tropical fruits, especially banana, in the Roditis wines, while phenylethyl acetate correlated with rose, vanilla and fruity notes in both the Moschofilero and Roditis samples. The Assyrtiko wines and the Malagousia wines were mainly associated with compounds such as cis-3-hexen-1-ol and cis- and trans-furan linalool oxides, which may enhance fresh fruit and citrus aromas through synergistic effects. The common background aroma of the studied wines was mainly determined by higher alcohols, fatty acids and ethyl esters. This study provides a basis for understanding the typical aroma of white wines from indigenous Greek grape varieties, which will help producers develop targeted wine styles and will be useful for consumer promotion. Full article

The objective of this research was the production of new sparkling wines using either commercial or native yeast strains along with traditional Italian grape varieties. This approach preserves the unique character of the region, represented by the typical grape varieties, and incorporates the concept of “microbial terroir”, which is linked to the native yeasts. The wines produced have been analyzed for conventional parameters such as pH, titratable acidity, volatile acidity, alcohol content, and residual sugar, as well as for their aromatic profile through GC-MS analysis. To evaluate the acceptance of the wines, a sensory analysis was conducted, and NIR spectra were collected to identify the factors influencing their preferences. Two varieties, Fiano and Falanghina, produced sparkling wines well appreciated with pleasant floral and white fruit notes. The positive reception of these innovative sparkling wines was connected to their chemical composition, offering valuable insights into the future production of similar products. Full article

‘Timorasso’ is an autochthonous, non-aromatic white grape variety cultivated mainly in the southwest of the Piedmont region (northwestern Italy). The sensory profile of wines produced from this variety evolves greatly with aging. In this study, 31 wines from 2015–2021 vintages were analyzed to investigate changes in sensory descriptors at various stages of aging and their correlation with physicochemical properties (wine basic parameters, color, and total polyphenols) and sensory-perceptual typicity. A sensory analysis was conducted by a panel of experts, who were asked to indicate the in-mouth and aroma descriptors. The aroma-related terms were analyzed as individual descriptors or grouped in “Categories”. Moreover, the panel rated the Color, In-Mouth, and Aroma typicity of these wines. ‘Timorasso’ based wines were found to have, on average, a relevant alcohol content (14.20 ± 0.56% v/v), moderate acidity (5.8 ± 0.6 g/L), and low pH (3.19 ± 0.09). In fact, Timorasso wines were sensorially identified in terms of citation frequency with the in-mouth descriptors acidity (32.9%), sapidity (25.5%), and minerality (17.4%). The aroma of younger wines (2 years of aging) was characterized by “Green”, “White flowers”, “White pulp fruit”, and “Citrus”. In general, the most cited aroma category was “Kerosene” (27.9%), distinguishing wines with 5–6 years of aging. “Kerosene” category correlated with Aroma typicity (p < 0.001), as well as with “Balsamic” (10.8%, p < 0.01) and “Empyreumatic” (5.5%, p < 0.05) aroma categories. Full article

This research aims to identify aroma compounds, their combinations, and statistical relationships to classify and characterize wines produced in small, defined areas known as “terroirs”, which share edaphoclimatic characteristics grape varieties, viticultural practices, harvest timing, and winemaking processes. The goal is to deepen the understanding of the relationship between the terroir and wine typicity. This study analyzed the contents based on enological parameters, the major and minor volatile compounds of the young wines produced in three wineries across two vintages, using the Pedro Ximenez white grape variety cultivated in different terroirs within the same quality zone. Statistical tools, such as Multiple Variable Analysis (MVA) and Principal Component Analysis (PCA), were employed to identify significant differences in the volatilomes of wines. PCA effectively differentiated wines from each terroir and vintage using scores from the first two principal components, calculated based on the absolute concentrations of 12 major volatile compounds and 3 polyols. Conversely, PCA based on the concentrations of 52 minor volatile compounds showed a strong ability to classify wines by vintage year. The minor volatile contents of wines from 2 vintages, grouped into 9 chemical families, provide distinct fingerprints that enable wines to be distinguished by terroir. The results underscore the chemical basis underlying terroir typicity in a production zone within a warm Protected Denomination of Origin (PDO). Full article

The polyphenol extraction and evolution during a traditional 14-day fermentation of the Aglianico red grape, a variety widely cultivated across Southern Italy, was for the first time investigated, with the purpose of optimizing the phenolic profile in finished wines. Anthocyanins, BSA-reactive tannins, iron-reactive phenols, and vanillin-reactive flavans (VRFs) were analyzed in the free-run must, pressed pomace liquid, and in pomace extracts at different maceration times. Experimental evidence suggested that, instead of the typical 14-day maceration of Aglianico grapes, it is recommendable to choose an 11-day maceration in order to prevent the over-extraction of polyphenols that may detrimentally affect the sensory characteristics of wines. In fact, Aglianico wines, if not properly produced, can be affected by excessive astringency due to its high tannin contents. The findings of the present study can provide insightful knowledge to all the winemakers dealing with grape varieties characterized by high quantities of tannins. Also, an earlier racking would supply grape pomaces extremely rich in valuable phenolic compounds to be extracted and reused in several industrial segments in the frame of circular bioeconomy. Full article

Wine lees, the second most significant by-product of winemaking after grape pomace, have received relatively little attention regarding their potential for valorization. Despite their rich content in bioactive components such as β-glucans, industrial utilization faces challenges, particularly due to variability in their composition. This inconsistency impacts the reliability and standardization of final products, limiting broader adoption in industrial applications. β-Glucans are dietary fibers or polysaccharides renowned for their diverse bioactive properties, including immunomodulatory, antioxidant, anti-inflammatory, antitumor, and cholesterol- and glucose-lowering effects. They modulate the immune system by activating Dectin-1 and TLR receptors on immune cells, enhancing phagocytosis, cytokine production, and adaptive immune responses. Their antioxidant activity arises from neutralizing free radicals and reducing oxidative stress, thereby protecting cells and tissues. β-Glucans also exhibit antitumor effects by inhibiting cancer cell growth, inducing apoptosis, and preventing angiogenesis, the formation of new blood vessels essential for tumor development. Additionally, they lower cholesterol and glucose levels by forming a viscous gel in the intestine, which reduces lipid and carbohydrate absorption, improving metabolic health. The biological activity of β-glucans varies with their molecular weight and source, further highlighting their versatility and functional potential. This study investigates how grape variety, vinification technology and extraction methods affect the yield and properties of β-glucans extracted from wine lees. The physico-chemical and mineral composition of different wine lees were analyzed, and two extraction methods of β-glucans from wine lees were tested: acid-base extraction and autolysis. These two methods were also tested under ultrasound-assisted conditions at different frequencies, as well as without the use of ultrasound. The β-glucan yield and properties were evaluated under different conditions. FTIR spectroscopy was used to assess the functional groups and structural characteristics of the β-glucans extracted from the wine lees, helping to confirm their composition and quality. Rheological behavior of the extracted β-glucans was also assessed to understand the impact of extraction method and raw material origin. The findings highlight that vinification technology significantly affects the composition of wine lees, while both the extraction method and yeast origin influence the yield and type of β-glucans obtained. The autolysis method provided higher β-glucan yields (18.95 ± 0.49% to 39.36 ± 0.19%) compared to the acid–base method (3.47 ± 0.66% to 19.76 ± 0.58%). FTIR spectroscopy revealed that the β-glucan extracts contain a variety of glucan and polysaccharide types, with distinct β-glucans (β-1,4, β-1,3, and β-1,6) identified through specific absorption peaks. The rheological behavior of suspensions exhibited pseudoplastic or shear-thinning behavior, where viscosity decreased significantly as shear rate increased. This behavior, observed across all β-glucan extracts, is typical of polymer-containing suspensions. These insights are critical for optimizing β-glucan extraction processes, supporting sustainability efforts and waste valorization in the wine industry. Efficient extraction of β-glucans from natural sources like wine lees offers a promising path toward their industrial application as valuable functional compounds. Full article

PIWI, from the German word Pilzwiderstandsfähig, meaning “fungus-resistant”, refers to grapevine cultivars bred for resistance to fungal pathogens such as Erysiphe necator (the causal agent of powdery mildew) and Plasmopara viticola (the causal agent of downy mildew), two major diseases in viticulture. These varieties are typically developed through traditional breeding, often crossbreeding European Vitis vinifera with American or Asian species that carry natural disease resistance. This study investigates the transcriptional profiles of exocarp tissues in mature berries from four PIWI grapevine varieties compared to their elite parental counterparts using RNA-seq analysis. We performed RNA-seq on four PIWI varieties (two red and two white) and their noble parents to identify differential gene expression patterns. Comprehensive analyses, including Differential Gene Expression (DEGs), Gene Set Enrichment Analysis (GSEA), Weighted Gene Co-expression Network Analysis (WGCNA), and tau analysis, revealed distinct gene clusters and individual genes characterizing the transcriptional landscape of PIWI varieties. Differentially expressed genes indicated significant changes in pathways related to organic acid metabolism and membrane transport, potentially contributing to enhanced resilience. WGCNA and k-means clustering highlighted co-expression modules linked to PIWI genotypes and their unique tolerance profiles. Tau analysis identified genes uniquely expressed in specific genotypes, with several already known for their defense roles. These findings offer insights into the molecular mechanisms underlying grapevine resistance and suggest promising avenues for breeding strategies to enhance disease resistance and overall grape quality in viticulture. Full article

In addition to winemaking techniques, wine’s quality and typicity are linked to the place where the grapes are grown. Climate, soil and the cultivar are major drivers of the terroir’s expression. Moreover, climate change is affecting the distribution of grapevine varieties in different wine-growing regions because changes in climatic conditions over the past years is affecting grape production. This study investigated the influence of some terroir parameters on the volatile composition of Cabernet Sauvignon wines. Eight wines from vineyards sited in Argentina, Spain and Portugal with altitudes between 2400 and 77 m above sea level (a.s.l.) with different soils and climatic conditions were selected. The results suggested that the vineyard’s place significantly modified the volatile composition of Cabernet Sauvignon wines because all chemical groups of volatiles quantified were affected. Volatile acids, C6 compounds, aldehydes, esters and terpenes showed the highest concentrations at 2400 m a.s.l., where the highest thermal amplitude was observed. In Portugal, where the vineyard studies were sited at lower altitudes (77 m a.s.l.) and under warm climatic conditions, high concentrations of alcohols and lactones were observed. Alcohols also showed high concentrations in wines from vineyards sited at 413 and 155 m a.s.l. (Spain). Principal component analysis showed the positive correlation of Cabernet Sauvignon wines located at higher altitudes with terpenes. On the contrary, wines from grapes grown at lower altitude conditions exhibited a correlation with alcohols and lactones. Full article