Search Results (975)

To explore the characteristics of species diversity and phylogenetic diversity, as well as the dominant processes of community construction, in different forest types (deciduous broad-leaved forest, mixed coniferous and broad-leaved forest, and Chinese fir plantation) in subtropical regions, analyze the specific driving patterns of soil nutrients and other environmental factors on the formation of forest diversity in different forest types, and clarify the differences in response to environmental heterogeneity between natural forests and plantation forests. Based on 48 fixed monitoring plots of 50 m × 50 m in Shouchang Forest Farm, Jiande City, Zhejiang Province, woody plants with a diameter at breast height ≥5 cm were investigated. Species diversity indices (Margalef index, Shannon–Wiener index, Simpson index, and Pielou index), phylogenetic structure index (PD), and environmental factors were used to analyze the relationship between diversity characteristics and environmental factors through variance analysis, correlation analysis, and generalized linear models. Phylogenetic structural indices (NRI and NTI) were used, combined with a random zero model, to explore the mechanisms of community construction in different forest types. Research has found that (1) the deciduous broad-leaved forest had the highest species diversity (Margalef index of 4.121 ± 1.425) and phylogenetic diversity (PD index of 21.265 ± 7.796), significantly higher than the mixed coniferous and broad-leaved forest and the Chinese fir plantation (p < 0.05); (2) there is a significant positive correlation between species richness and phylogenetic diversity, with the best fit being AIC = 70.5636 and R² = 0.9419 in broad-leaved forests; however, the contribution of evenness is limited; (3) the specific effects of soil factors on different forest types: available phosphorus (AP) is negatively correlated with the diversity of deciduous broad-leaved forests (p < 0.05), total phosphorus (TP) promotes the diversity of coniferous and broad-leaved mixed forests, while the diversity of Chinese fir plantations is significantly negatively correlated with total nitrogen (TN); (4) the phylogenetic structure of three different forest types shows a divergent pattern in deciduous broad-leaved forests, indicating that competition and exclusion dominate the construction of deciduous broad-leaved forests; the aggregation mode of Chinese fir plantation indicates that environmental filtering dominates the construction of Chinese fir plantation; the mixed coniferous and broad-leaved forest is a transitional model, indicating that the mixed coniferous and broad-leaved forest is influenced by both stochastic processes and ecological niche processes. In different forest types in subtropical regions, the species and phylogenetic diversity of broad-leaved forests is significantly higher than in other forest types. The impact of soil nutrients on the diversity of different forest types varies, and the characteristics of community construction in different forest types are also different. This indicates the importance of protecting the original vegetation and provides a scientific basis for improving the ecological function of artificial forest ecosystems through structural adjustment. The research results have important practical guidance value for sustainable forest management and biodiversity conservation in the region. Full article

The somatic cell count (SCC) and differential somatic cell count (DSCC) are proxies for the udder health of dairy cattle, regarded as the criterion of mastitis identification with healthy, suspicious mastitis, mastitis, and chronic/persistent mastitis. However, SCC and DSCC are tested using flow cytometry, which is expensive and time-consuming, particularly for DSCC analysis. Mid-infrared spectroscopy (MIR) enables qualitative and quantitative analysis of milk constituents with great advantages, being cheap, non-destructive, fast, and high-throughput. The objective of this study is to develop a dairy cattle udder health status diagnostic model of MIR. Data on milk composition, SCC, DSCC, and MIR from 2288 milk samples collected in dairy farms were analyzed using the CombiFoss 7 DC instrument (FOSS, Hilleroed, Denmark). Three MIR spectral preprocessing methods, six modeling algorithms, and three different sets of MIR spectral data were employed in various combinations to develop several diagnostic models for mastitis of dairy cattle. The MIR diagnostic model of effectively identifying the healthy and mastitis cattle was developed using a spectral preprocessing method of difference (DIFF), a modeling algorithm of Random Forest (RF), and 1060 wavenumbers, abbreviated as “DIFF-RF-1060 wavenumbers”, and the AUC reached 1.00 in the training set and 0.80 in the test set. The other MIR diagnostic model of effectively distinguishing mastitis and chronic/persistent mastitis cows was “DIFF-SVM-274 wavenumbers”, with an AUC of 0.87 in the training set and 0.85 in the test set. For more effective use of the model on dairy farms, it is necessary and worthwhile to gather more representative and diverse samples to improve the diagnostic precision and versatility of these models. Full article

A reliable estimation of protein requirements in lactating dairy cows is necessary for formulating nutritionally adequate diets, improving feed efficiency, and minimizing nitrogen excretion. This study aimed to develop machine learning-based models to predict net protein requirements for maintenance (NPm) and lactation (NPl) using random forest regression (RFR) and support vector regression (SVR). A total of 1779 observations were assembled from 436 peer-reviewed publications and open-access databases. Predictor variables included farm-ready variables such as milk yield, dry matter intake, days in milk, body weight, and dietary crude protein content. NPm was estimated based on the National Academies of Sciences, Engineering, and Medicine (NASEM, 2021) equations, while NPl was derived from milk true protein yield. The model adequacy was evaluated using 10-fold cross-validation. The RFR model demonstrated higher predictive performance than SVR for both NPm (R² = 0.82, RMSEP = 22.38 g/d, CCC = 0.89) and NPl (R² = 0.82, RMSEP = 95.17 g/d, CCC = 0.89), reflecting its capacity to model the rule-based nature of the NASEM equations. These findings suggest that RFR may provide a valuable approach for estimating protein requirements with fewer input variables. Further research should focus on validating these models under field conditions and exploring hybrid modeling frameworks that integrate mechanistic and machine learning approaches. Full article

Escherichia coli (E. coli) is an opportunistic pathogen widely distributed in nature, and multi-drug resistance (MDR) E. coli has been widely recognized as a critical reservoir of resistance genes, posing severe health threats to humans and animals. A total of 288 E. coli strains were isolated and purified from fresh fecal samples of forest musk deer collected from farms in Sichuan, Shaanxi, and Yunnan Provinces of China between 2013 and 2023. This study aimed to conduct antibiotic susceptibility testing and resistance gene detection on the isolated forest musk deer-derived E. coli, analyze the correlations between them, investigate the presence of CRISPR systems within the strains, and perform bioinformatics analysis on the CRISPR systems carried by the strains. Results showed that 138 out of 288 E. coli strains were MDR, with the highest resistance to tetracycline (48.3%), cefalexin (45.1%), and doxycycline (41.7%). Prevalent genes were tetA (41.0%), sul2 (30.2%), blaTEM (27.1%), with 29 gene–phenotype pairs correlated. CRISPR system-negative strains had higher resistance rates to 16 antibiotics and lower detection rates only for aac (6′)-Ib-cr, qnrA, and qnrB compared to CRISPR system-positive strains. Regional analysis showed that the problem of drug resistance in Sichuan and Shaanxi was more serious, and that the detection rate of antibiotic resistance genes was relatively high. This study guides E. coli infection control in forest musk deer and enriches resistance research data. Full article

Tree height is a critical indicator for estimating forest stock and can be effectively acquired by UAV-LiDAR. Ground filtering works to classify ground points and non-ground points and can impact the tree height extraction results, while the points classification quality obtained by ordinary filtering methods is limited in complex forest conditions. A partitioned cloth simulation filtering (PCSF) method based on different vegetation cover was proposed in this study to improve the classification accuracy, and tree heights were extracted to demonstrate the effectiveness of the proposed method. UAV-LiDAR data and field measurements collected from the Lutou experimental forest farm in the southern subtropical forest region of China were used for validation, and the slope-based filtering, progressive triangulated irregular network densification filtering (PTD), moving surface fitting filtering (MSFF), and CSF were adopted for comparisons. The results showed that the proposed method yielded the best ground filtering effect, reducing the filtering total error by 2.12%–4.22% compared with other methods, and the relative root mean squared error (rRMSE) of extracted tree heights was reduced by 1.24%–3.84%, respectively. The proposed method can achieve a satisfactory filtering effect and tree height extraction result, which provides a methodological basis to precisely extract tree heights in large-scale forests. Full article

Soil available nitrogen (AN) is a critical nutrient for plant absorption and utilization. Accurately mapping its spatial distribution is essential for improving crop yields and advancing precision agriculture. In this study, 188 AN soil samples (0–20 cm) were collected at Heshan Farm, Nenjiang County, Heihe City, Heilongjiang Province, in 2023. The soil available nitrogen content ranged from 65.81 to 387.10 mg kg⁻¹, with a mean value of 213.85 ± 61.16 mg kg⁻¹. Sentinel-2 images and normalized vegetation index (NDVI) and enhanced vegetation index (EVI) time series data were acquired on the Google Earth Engine (GEE) platform in the study area during the bare soil period (April, May, and October) and the growth period (June–September). These remote sensing variables were combined with soil sample data, crop type information, and crop growth period data as predictive factors and input into a Random Forest (RF) model optimized using the Optuna hyperparameter tuning algorithm. The accuracy of different strategies was evaluated using 5-fold cross-validation. The research results indicate that (1) the introduction of growth information at different growth periods of soybean and maize has different effects on the accuracy of soil AN mapping. In soybean plantations, the introduction of EVI data during the pod setting period increased the mapping accuracy R² by 0.024–0.088 compared to other growth periods. In maize plantations, the introduction of EVI data during the grouting period increased R² by 0.004–0.033 compared to other growth periods, which is closely related to the nitrogen absorption intensity and spectral response characteristics during the reproductive growth period of crops. (2) Combining the crop types and their optimal period growth information could improve the mapping accuracy, compared with only using the bare soil period image (R² = 0.597)—the R² increased by 0.035, the root mean square error (RMSE) decreased by 0.504%, and the mapping accuracy of R² could be up to 0.632. (3) The mapping accuracy of the bare soil period image differed significantly among different months, with a higher mapping accuracy for the spring data than the fall, the R² value improved by 0.106 and 0.100 compared with that of the fall, and the month of April was the optimal window period of the bare soil period in the present study area. The study shows that when mapping the soil AN content in arable land, different crop types, data collection time, and crop growth differences should be considered comprehensively, and the combination of specific crop types and their optimal period growth information has a greater potential to improve the accuracy of mapping soil AN content. This method not only opens up a new technological path to improve the accuracy of remote sensing mapping of soil attributes but also lays a solid foundation for the research and development of precision agriculture and sustainability. Full article

In view of the complex operating environments of wind farms and the characteristics of multi-branch mixed collector lines, in order to improve the accuracy of single-phase grounding fault location, the convolutional neural network (CNN), bidirectional long short-term memory network (BiLSTM), and attention mechanism (attention) were combined to construct a single-phase grounding fault location strategy for the CNN–BiLSTM–attention hybrid model. Using a zero-sequence current as the fault information identification method, through the deep fusion of the CNN–BiLSTM–attention hybrid model, the single-phase grounding faults in the collector lines of the wind farm can be located. The simulation modeling was carried out using the MATLAB R2022b software, and the effectiveness of the hybrid model in the single-phase grounding fault location of multi-branch mixed collector lines was studied and verified. The research results show that, compared with the random forest algorithm, decision tree algorithm, CNN, and LSTM neural network, the proposed method significantly improved the location accuracy and is more suitable for the fault distance measurement requirements of collector lines in the complex environments of wind farms. The research conclusions provide technical support and a reference for the actual operation and maintenance of wind farms. Full article

The Chinese mitten crab (Eriocheir sinensis) industry is currently facing the challenges of origin fraud, as well as a lack of precision and interpretability of existing traceability methods. Here, we propose a high-precision origin traceability method based on a combination of stable isotope analysis and interpretable machine learning. We sampled Chinese mitten crabs from six origins representing diverse aquatic environments and farming practices, and analyzed their δ¹³C, δ¹⁵N, δ²H, and δ¹⁸O stable isotope compositions in different sexes and tissues (hepatopancreas, muscle, and gonad). By comparing the classification performance of Random Forest, XGBoost, and Logistic Regression models, we found that the Random Forest model outperformed the others, achieving high accuracy (91.3%) in distinguishing samples from different origins. Interpretation of the optimal Random Forest model, using SHAP (SHapley Additive exPlanations) analysis, identified δ²H in male muscle, δ¹⁵N in female hepatopancreas, and δ¹³C in female hepatopancreas as the most influential features for discriminating geographic origin. This analysis highlighted the crucial role of environmental factors, such as water source, diet, and trophic level, in origin discrimination and demonstrated that isotopic characteristics of different tissues provide unique discriminatory information. This study offers a novel paradigm for stable isotope traceability based on explainable machine learning, significantly enhancing the identification capability and reliability of Chinese mitten crab origin traceability, and holds significant implications for food safety assurance. Full article

Soil degradation and declining fertility threaten sustainable agriculture and crop productivity. This study evaluates the effects of CFMI-8, a co-fermented microbial inoculant comprising eight bacterial strains selected through genomic and metabolic modeling, on soil health, nutrient availability, and corn performance. Conducted in a randomized complete block design at Findlay Farm, Wisconsin, the field trial assessed soil biological activity, nutrient cycling, and crop yield responses to CFMI-8 treatment. Treated soils exhibited significant increases in microbial organic carbon (+224.1%) and CO₂ respiration (+167.1%), indicating enhanced microbial activity and organic matter decomposition. Improvements in nitrate nitrogen (+20.2%), cation exchange capacity (+23.1%), and potassium (+27.3%) were also observed. Corn yield increased by 28.6%, with corresponding gains in silage yield (+9.6%) and nutritional quality. Leaf micronutrient concentrations, particularly iron, manganese, boron, and zinc, were significantly higher in treated plants. Correlation and Random Forest analyses identified microbial activity and nitrogen availability as key predictors of yield and nutrient uptake. These results demonstrate CFMI-8’s potential to enhance soil fertility, promote nutrient cycling, and improve crop productivity under field conditions. The findings support microbial inoculants as viable tools for regenerative agriculture and emphasize the need for long-term studies to assess sustainability impacts. Full article

The aim of this review was to present selected machine learning (ML) algorithms used in dairy cattle farming in recent years (2020–2024). A description of ML methods (linear and logistic regression, classification and regression trees, chi-squared automatic interaction detection, random forest, AdaBoost, support vector machines, k-nearest neighbors, naive Bayes classifier, multivariate adaptive regression splines, artificial neural networks, including deep neural networks and convolutional neural networks, as well as Gaussian mixture models and cluster analysis), with some examples of their application in various aspects of dairy cattle breeding and husbandry, is provided. In addition, the stages of model construction and implementation, as well as the performance indicators for regression and classification models, are described. Finally, time trends in the popularity of ML methods in dairy cattle farming are briefly discussed. Full article

The change in the socio-political formation of Russia from a socialist planned system to a capitalist market system significantly influenced agriculture and one of its components—arable land. The loss of the sustainability of land management for arable land led to a reduction in sown areas by 38% (from 119.7 to 74.7 million ha) and a synchronous drop in gross harvests of grain and leguminous crops by 48% (from 117 to 61 million tons). The situation stabilized in 2020, with a sowing area of 80.2 million ha and gross harvests of grain and leguminous crops of 120–150 million tons. This process was not formalized legally, and the official (legal) area of arable land decreased by only 8% from 132.8 to 122.3 million ha. Legal conflict arose for 35 million ha for unused arable land, for which there was no classification of its condition categories and no monitoring of the withdrawal time of the arable land from actual agricultural use. The aim of this study was to resolve the challenges in the method of retrospective monitoring of soil–land cover, which allowed for the achievement of the aims of the investigation—to elucidate the history of land use on arable lands from 1985 to 2025 with a time step of 5 years and to obtain a detailed classification of the arable lands’ abandonment degrees. It was also established that on most of the abandoned arable land, carbon sequestration occurs in the form of secondary forests. In the course of this work, it was shown that the reasons for the formation of an array of abandoned arable land and the stabilization of agricultural production turned out to be interrelated. The abandonment of arable land occurred proportionally to changes in the soil’s natural fertility and the degree of land degradation. Economically unprofitable lands spontaneously (without centralized planning) left the sowing zone. The efficiency of land use on the remaining lands has increased and has allowed for the mass application of modern farming systems (smart, precise, landscape-adaptive, differentiated, no-till, strip-till, etc.), which has further increased the profitability of crop production. The prospect of using abandoned lands as a carbon sequestration zone in areas of forest overgrowth has arisen. Full article

The increasing deployment of wind energy systems, particularly offshore wind farms, necessitates advanced monitoring and maintenance strategies to ensure optimal performance and minimize downtime. Supervisory Control And Data Acquisition (SCADA) systems have become indispensable tools for monitoring the operational health of wind turbines, generating vast quantities of time series data from various sensors. Anomaly detection techniques applied to this data offer the potential to proactively identify deviations from normal behavior, providing early warning signals of potential component failures. Traditional model-based approaches for fault detection often struggle to capture the complexity and non-linear dynamics of wind turbine systems. This has led to a growing interest in data-driven methods, particularly those leveraging machine learning and deep learning, to address anomaly detection in wind energy applications. This study focuses on the development and application of a semi-supervised, multivariate anomaly detection model for horizontal axis wind turbines. The core of this study lies in Bidirectional Long Short-Term Memory (BI-LSTM) networks, specifically a BI-LSTM autoencoder architecture, to analyze time series data from a SCADA system and automatically detect anomalous behavior that could indicate potential component failures. Moreover, the approach is reinforced by the integration of the Isolation Forest algorithm, which operates in an unsupervised manner to further refine normal behavior by identifying and excluding additional anomalous points in the training set, beyond those already labeled by the data provider. The research utilizes a real-world dataset provided by EDP Renewables, encompassing two years of comprehensive SCADA records collected from a single offshore wind turbine operating in the Gulf of Guinea. Furthermore, the dataset contains the logs of failure events and recorded alarms triggered by the SCADA system across a wide range of subsystems. The paper proposes a multi-modal anomaly detection framework orchestrating an unsupervised module (i.e., decision tree method) with a supervised one (i.e., BI-LSTM AE). The results highlight the efficacy of the BI-LSTM autoencoder in accurately identifying anomalies within the SCADA data that exhibit strong temporal correlation with logged warnings and the actual failure events. The model’s performance is rigorously evaluated using standard machine learning metrics, including precision, recall, F1 Score, and accuracy, all of which demonstrate favorable results. Further analysis is conducted using Cumulative Sum (CUSUM) control charts to gain a deeper understanding of the identified anomalies’ behavior, particularly their persistence and timing leading up to the failures. Full article

Urbanisation and land-use change are among the main pressures on soil health in periurban areas, but the multifunctionality of grassland soils is still not sufficiently recognised. In this study, the physical and chemical properties of soils under grassland, forest and croplands in the periurban area of Zagreb were investigated in a two-year period. Grasslands consistently exhibited multifunctional benefits, including high organic matter content (4.68% vs. 2.24% in cropland), improved bulk density (1.14 vs. 1.24 g cm⁻³) and an active carbon cycle indicated by increased CO₂ emissions (up to 1403 kg ha⁻¹ day⁻¹ in 2021). Forest soils showed the highest aggregate stability (91.4%) and infiltration (0.0006 cm s⁻¹), while croplands showed signs of structural degradation with the highest bulk density and lowest water retention (39.9%). Temporal variation showed that grassland was particularly responsive to favourable climatic conditions, with soil porosity and water content improving yearly. Principal component analysis showed that soil structure, biological activity and moisture regulation were linked, with grassland plots favourably positioned along the axes of resilience. The absence of tillage and the presence of permanent vegetation cover contributed to their high capacity for climate and water regulation and carbon sequestration. These results emphasise the importance of protecting and managing grasslands as an important component of urban green areas. Practices such as mulching, minimal disturbance and continuous cover can maximise the ecosystem services of grassland soils. In addition, the results highlight the potential risk of trace metal accumulation in cropland and grassland soils located near urban and farming infrastructure, underlining the need for regular monitoring in periurban environments. Integrating grassland functions into urban planning and policy is essential for improving the sustainability and resilience of periurban landscapes. Full article

Water is scarce in semi-arid regions due to environmental limitations; this situation is aggravated by changes in land use and land cover (LULC). In this respect, the basic ecological functions of Permanent Preservation Areas (PPAs) help to maintain water resources. The aim of this study was to evaluate the relationship between the LULC and water quality in PPAs in a semi-arid watershed, from 2009 to 2016. The following limnological data were analyzed: chlorophyll-a, transparency, total nitrogen and total phosphorus. The changes in LULC were obtained by classifying images from Landsat 5, 7 and 8 into three types: Open Dry Tropical Forest (ODTF), Dense Dry Tropical Forest (DDTF) and Exposed Soil (ES). Spearman correlation and principal component analysis were applied to evaluate the relationships between the parameters. There was a significant positive correlation between DDTF and the best limnological conditions. However, ES showed a significant negative relationship with transparency and a positive relationship with chlorophyll-a, indicating a greater input of sediments and nutrients into the water. The PCA corroborated the results of the correlation. It is therefore essential to prioritize the preservation and restoration of the vegetation in these sensitive areas to ensure the sustainability of water resources. Future studies should assess the impact of specific human activities, such as agriculture, deforestation and livestock farming, on water quality in the PPAs. Full article

The coastal region of Klaipėda (Lithuania) has experienced major political, economic, social, and cultural transformations since the 20th century. Landscapes as evolving expressions of land use and land cover patterns offer a valuable lens to analyse these changes. This study examines the evolution of physical landscape structures across the pre-Soviet, Soviet, and post-Soviet periods, using historical maps and open-access geospatial data. An ontological approach, combined with morphological and configurational metrics, reveals four major and relatively persistent landscape structures: hydrological systems (sea, lagoon, rivers), forest cover, farming intensity (from extensive grassland use to intensive arable farming), and semi-natural environments. Their structural evolution reflects broader cultural factors, such as contrasting land use traditions between former Prussian and Russian territories. The study also highlights the impact of Soviet collectivisation, marked by irrigation networks, agricultural intensification, and forest expansion. The post-Soviet period is characterised by widespread farmland abandonment and fragmentation, revealing new spatial dynamics and challenges in land reappropriation. Landscape transformations are predominantly structured around agricultural dynamics. Although the analysis was limited by the incomplete availability of data for this specific land use class, the centrality of agriculture in shaping territorial organisation is evident and reinforces the strong rural identity associated with the landscape. Full article