Search Results (380)

The climbing perch, Anabas testudineus, is one of the most widely distributed freshwater amphibious fishes in South and Southeast Asia, exhibiting terrestrial movements. Our experimental study aimed to investigate endocrinological and biochemical changes in the blood of climbing perch associated with their terrestrial movements. To achieve this, the fish were divided into two groups: one group was exposed to aquatic conditions for twenty minutes, while the other group was subjected to terrestrial conditions for the same duration through rapid water level decrease. In terrestrial conditions, the fish predominantly exhibit movements on land, whereas in aquatic environments, they primarily remain immobile or swim. Elevated levels of stress-induced cortisol and glucose after short-term exposure indicate a high-stress response involving both neuroendocrine and metabolic mechanisms. Changes in the activity of aspartate aminotransferase and increased concentrations of triglycerides in the blood serum suggest energy mobilization through aerobic metabolic pathways. Extreme environmental changes did not affect thyroid axis function, including deiodination, thereby maintaining essential physiological activities under new conditions. Additionally, the anaerobic metabolic pathway appears to be minimally utilized at the onset of terrestrial movement, as no significant changes in lactate dehydrogenase concentrations were observed. Overall, the terrestrial movements of the climbing perch are likely predominantly forced and associated with high stress. Full article

Background: Optimal nutrition and training regimens are essential for athletes to maximize performance and recovery. Probiotic supplementation, through the modulation of the gut microbiota, and omega-3 fatty acids, known for their anti-inflammatory properties, may enhance physiological adaptations when combined with targeted training. This study evaluated the effects of probiotics and omega-3 supplementation, alongside ultra-short race pace training (USRPT), on performance metrics in competitive sprint swimmers. Methods: In this double-blind, placebo-controlled study, 60 male sprint swimmers (age: 19.2 ± 3.6 years; height: 182.2 ± 5.2 cm; weight: 81.6 ± 4.4 kg) with a minimum of five years of training experience, were randomly assigned to six groups (n = 10 per group): (1) Control (CON), (2) USRPT only, (3) Placebo + USRPT (PLA + USRPT), (4) Probiotics + USRPT (PRO + USRPT), (5) Omega-3 + USRPT (OMEGA + USRPT), and (6) Probiotics + Omega-3 + USRPT (PRO + OMEGA + USRPT). Over the eight-week intervention, the participants in PRO + USRPT consumed one multi-strain probiotic capsule daily (4.5 × 10¹¹ CFU) and a placebo capsule. Those in OMEGA + USRPT ingested 1000 mg of fish oil after lunch (500 mg EPA and 180 mg DHA per capsule) paired with a placebo capsule. The combined supplementation group (PRO + OMEGA + USRPT) received both probiotic and omega-3 capsules. The PLA + USRPT group consumed two starch capsules daily. The USRPT protocol was implemented across all the training groups, where the swimmers performed 17 sets of 25 m and 12.5 m sprints based on weekly recorded race times. Performance assessments included pre- and post-test measurements of sprint times (50 m and 100 m freestyle), vertical jump tests (both in water and on dry land), and other strength and endurance metrics (reaction time, agility T-test, sprint index, fatigue index, and velocity). Results: The combined intervention of probiotics and omega-3 with USRPT produced the greatest improvements in performance. The PRO + OMEGA + USRPT group reduced 50 m freestyle time by 1.92% (p = 0.002, pEta² = 0.286) and 100 m freestyle time by 2.48% (p = 0.041, pEta² = 0.229), demonstrating significant Time × Group interactions consistent with a synergistic effect. Additionally, the sprint index improved (pEta² = 0.139, p = 0.013) and reaction time decreased (pEta² = 0.241, p = 0.009) in the combined group, indicating enhanced anaerobic capacity and neuromuscular responsiveness compared to single interventions. Conclusions: This study suggests that combining probiotics and omega-3 supplementation with USRPT leads to synergistic improvements in sprint swimming performance, enhancing anaerobic power and recovery beyond what is achieved with individual interventions. This integrated approach may provide a practical strategy for competitive swimmers seeking to optimize their performance. Future studies should incorporate mechanistic markers, longer intervention durations, and diverse athlete populations to clarify further and extend these findings. Full article

The gel-forming ability of collagens is vital for their application in cell scaffolds, yet very few comparative studies on fish collagen sources are available. This study isolated and characterized type I collagens from carp skin (CSK), scales (CSC), and swim bladders (CSB) and sturgeon skin (SSK) and swim bladders (SSB). The carp collagens exhibited higher thermal stability (34.75–34.78 °C) and formed more transparent, stronger gels than the sturgeon collagens. Additionally, as demonstrated by scanning electron microscopy, the sturgeon collagens exhibited faster fibril formation, with visible fibrils after 3 h which grew thicker but did not form bundles. The carp collagens, in contrast, initially displayed fewer, thinner, and longer fibrils, with their formation accelerating over time and fibril bundles emerging after 24 h. All collagen solutions of 4% (w/v) exhibited shear-thinning flow behavior, with the carp-derived solutions showing higher viscosities (10³–10⁴ Pa·s) than those demonstrated by the sturgeon-derived solutions (10²–10³ Pa·s). The CSBs and SSBs demonstrated the highest storage (G′) and loss (G″) moduli, with the former exhibiting the lowest loss tangent (tan δ), indicative of a stronger gel structure. The gels at 24 h showed slightly poorer mechanical properties than those at 3 h. The CSC and SSB gels had the highest thermal stability. These findings highlight the distinctiveness of the characteristics of collagens and their gels, emphasizing their potential in biomaterial applications. The present study also provides a foundational framework for assessing cellular responses in a comparative context that may help in identifying the most suitable collagen types for biomedical applications. Full article

This paper introduces a novel bio-inspired metaheuristic algorithm, named the sharpbelly fish optimizer (SFO), inspired by the collective ecological behaviors of the sharpbelly fish. The algorithm integrates four biologically motivated strategies—(1) fitness-driven fast swimming, (2) convergence-guided gathering, (3) stagnation-triggered dispersal, and (4) disturbance-induced escape—which synergistically enhance the balance between global exploration and local exploitation. To assess its performance, the proposed SFO is evaluated on the CEC2022 benchmark suite under various dimensions. The experimental results demonstrate that SFO consistently achieves competitive or superior optimization accuracy and convergence speed compared to seven state-of-the-art metaheuristic algorithms. Furthermore, the algorithm is applied to three classical constrained engineering design problems: pressure vessel, speed reducer, and gear train design. In these applications, SFO exhibits strong robustness and solution quality, validating its potential as a general-purpose optimization tool for complex real-world problems. These findings highlight SFO’s effectiveness in tackling nonlinear, constrained, and multimodal optimization tasks, with promising applicability in diverse engineering scenarios. Full article

Effective monitoring of fish passage through river barriers is essential for evaluating fishway performance and supporting adaptive river management. Traditional methods are often invasive, labor-intensive, or too costly to enable widespread implementation across most fishways. Infrared (IR) beam-break counters offer a promising alternative, but their adoption has been limited by high costs and a lack of flexibility. We developed and tested a novel, low-cost infrared beam-break counter—FishTracker—based on open-source Raspberry Pi and Arduino platforms. The system detects fish passages by analyzing interruptions in an IR curtain and reconstructing fish silhouettes to estimate movement, direction, speed, and morphometrics under a wide range of turbidity conditions. It also offers remote access capabilities for easy management. Field validation involved controlled tests with dummy fish, experiments with small-bodied live specimens (bleak) under varying turbidity conditions, and verification against synchronized video of free-swimming fish (koi carp). This first version of FishTracker achieved detection rates of 95–100% under controlled conditions and approximately 70% in semi-natural conditions, comparable to commercial counters. Most errors were due to surface distortion caused by partial submersion during the experimental setup, which could be avoided by fully submerging the device. Body length estimation based on passage speed and beam-interruption duration proved consistent, aligning with published allometric models for carps. FishTracker offers a promising and affordable solution for non-invasive fish monitoring in multispecies contexts. Its design, based primarily on open technology, allows for flexible adaptation and broad deployment, particularly in locations where commercial technologies are economically unfeasible. Full article

Understanding inter-species differences in behavioral lateralization and exploration patterns is crucial for advancing the study of animal behavior. In this study, we standardized the experimental procedure to minimize methodological variability and examined the behavioral responses of eight fish species (Girardinus falcatus, Poecilia reticulata, Paracheidon axelrodi, Kriptoterus bichirris, Hyphessobrycon megalopterus, Danio rerio, Corydoras aeneus, and Xenopoecilius sarasinorum) in a novel, circular environment. We focused on boldness-related measures (latency to explore and freezing time) and motor activity (circular vs. linear swimming). Significant inter-species differences were observed in the boldness measures. Fish also showed a preference for circular swimming over linear swimming. However, no lateralization bias (clockwise vs. counterclockwise) was detected in any species. These findings may establish a baseline for future research on the spontaneous behaviors of eight teleost species and offer valuable insights for the design of future behavioral studies focusing on these species. Full article

Trachinotus ovatus is a euryhaline, warm-water pelagic fish species with strong adaptability, rapid growth, and a high survival rate, making it one of the most important marine aquaculture species in China. In recent years, extensive experience has been accumulated in the cage farming of T. ovatus, but whether it can adapt to deep-sea environments and grow normally remains a current research focus. This study used RNA-Seq sequencing technology to analyze the gene expression changes in the liver of T. ovatus under three conditions: rest (0 cm/s), medium flow velocity (54 cm/s), and high flow velocity (90 cm/s). Through differential expression analysis, Short Time-series Expression Miner (STEM) analysis and protein–protein interaction (PPI) network analysis, a total of 5107 differentially expressed genes (DEGs), three significantly expressed gene profiles (profile6, profile1, and profile5), and 15 hub genes were identified. The results showed that changes in flow speed significantly impacted key biological processes such as energy metabolism, protein homeostasis, and endoplasmic reticulum (ER) stress response. Under moderate and high flow conditions, glycolysis-related genes were upregulated to meet the energy demands of swimming, while the downregulation of the PPARγ-RXRG complex and its downstream genes in the lipid metabolism pathway suggested a limitation in its fatty acid β-oxidation capacity. At the same time, protein synthesis was enhanced, and the unfolded protein response (UPR) was activated to help cope with ER stress. Furthermore, when the flow speed reached 90 cm/s, the expression of UPR- related genes and the anti-apoptotic factor JNK significantly decreased, suggesting that the stress response was nearing its limit and could potentially trigger cell apoptosis. These findings provide new insights into the molecular adaptation mechanisms of T. ovatus to flow speed stress and offer theoretical support for its rational farming in deep-sea cages, suggesting that the water flow speed in farming should not exceed 90 cm/s. Full article

Swimming is crucial to fish survival and reproduction, and is usually influenced by species, morphology, habitat environment and physiological condition. Understanding the changes in fish swimming ability and identifying the driving factors behind these changes is vital. In this study, seven endemic fish species from the Qingshui River, an important tributary of the upper Pearl River in Southwest China, were used to explore the effects of biological and environmental factors on fish swimming ability. The results indicated that the body length parameters had a minimal effect on the induced swimming speed (U_ind) but played a positive role in critical and burst swimming ability (U_crit and U_burst). Weight (W) and W/SL (the ratio of weight to standard body length) correlated more highly with swimming ability than body length parameters. Fish preferring the lotic environment exhibited higher U_crit and U_burst, and fish with a streamlined morphology had a smaller U_ind. The U_ind, U_ind/SL, U_ind/W and U_ind/(W/SL) of Discogobio yunnanensis (Regan, 1907) and Pseudocrossocheilus tridentis (Cui & Chu, 1986) were significantly higher during the spawning period. Acrossocheilus yunnanensis (Regan, 1904) performed better during the spawning period in U_crit and U_crit/SL. The U_burst of fish during the spawning period was smaller than that during the non-spawning period. Generally, the species difference had the greatest contribution to the swimming ability difference, followed by environmental conditions and fish morphology. Full article

Real-time estimation of fish growth offers multiple benefits in indoor aquaculture, including reduced labor, lower operational costs, improved feeding efficiency, and optimized harvesting schedules. This study presents a low-cost, vision-based method for estimating the body length and weight of olive flounder (Paralichthys olivaceus) in tank environments. A 5 × 5 cm reference grid is placed on the tank bottom, and images are captured using two fixed-position RGB smartphone cameras. Pixel measurements from the images are converted into millimeters using a calibrated pixel-to-length relationship. The system calculates fish length by detecting contour extremities and applying Lagrange interpolation. Based on the estimated length, body weight is derived using a power regression model. Accuracy was validated using both manual length measurements and Bland–Altman analysis, which indicated a mean bias of −0.007 cm and 95% limits of agreement from −0.475 to +0.462 cm, confirming consistent agreement between methods. The mean absolute error (MAE) and mean squared error (MSE) were 0.11 cm and 0.025 cm², respectively. While optimized for benthic species such as olive flounder, this system is not suitable for free-swimming species. Overall, it provides a practical and scalable approach for non-invasive monitoring of fish growth in commercial indoor aquaculture. Full article

The adverse impacts of invasive salmonids on native galaxiids are well documented at the population level in the freshwater ecosystems of the Southern Hemisphere. However, the mechanism underlying these interactions and sub-lethal effects of salmonids on native galaxiids at the individual level remain poorly understood. In this study, a series of controlled experiments was conducted to assess sub-lethal interactions between invasive brown trout (Salmo trutta) and the native Galaxias platei at an individual level. The microhabitat preferences of G. platei were evaluated in response to potential competition with juvenile brown trout and predation risk from piscivorous adults. In addition, the swimming capacity of G. platei was assessed to determine their ability to escape predation. The results show that at increasing densities of juvenile brown trout, G. platei fails to increase refuge use and are more frequently observed in open habitats. Furthermore, G. platei juveniles exhibit significantly lower swimming capacity compared to brown trout. In the presence of predatory trout, G. platei does not display a heightened preference for refuge habitats. These findings suggest that the behavioral response of G. platei could be insufficient to reduce competition and predation risks posed by brown trout and potentially other salmonids. Full article

As a soft actuator for fish robots, an oblique cone dielectric elastomer actuator (DEA) module inspired by the structure of white muscles in fish was proposed in the authors’ previous study. However, a mathematical model of an oblique cone DEA was not established, and designing a drive module that took into account its driving characteristics and passivity for integration into a fish robot remained a challenge. The purpose of this paper is to develop a vertebrate fish robot using multiple oblique cone DEA modules to achieve fish-like bending capability. First, an oblique cone DEA module was modeled for the design of a fish robot. The relationships among bending angle, blocking torque, driving voltage, and design parameters were established and confirmed by comparing the calculated and experimental results. Based on the modeling results, we designed an oblique cone DEA module-connected vertebrate fish robot. Finally, the experimental results of the fabricated fish robot demonstrated that the model-based design enabled flexible body swinging and swimming through a multiple-module-connected vertebrate structure. Full article

Compared with real fish, bionic fish have significant gaps in terms of swimming speed and efficiency, turning performance, and agility. The complicated underwater working environment necessitates monitoring equipment that can deal with the dynamic interference of dense fish schools and aquatic vegetation. An agile and flexible bionic fish with a fast swimming speed would be better suited to underwater monitoring tasks. In this study, a bionic greenfin fish robot is designed in detail, and a hydrodynamic simulation analysis of the designed bionic greenfin fish robot is carried out using STAR CCM+ and Fluent software to analyze the effects of different parameters on the propulsion performance of the pectoral fins, the steering of the caudal fins, and the emergency stop function. The swimming efficiency was found to be highest when the angle of attack was changed sinusoidally by 10° and the frequency was the same as that of the pectoral fin flutter. The feasibility of an emergency stop of the tail fin with negative-phase swinging and the adjustment of the pectoral fin uneven flutter monitoring position were also confirmed. Full article

This study aims to clarify the influence of water flow on the behavior of the large yellow croaker (Larimichthys crocea). Although L. crocea is a key species in marine cage aquaculture, and the industry is increasingly adopting large-scale sea cages, the behavioral adaptations of this species under such conditions remain insufficiently characterized. To solve this problem, the study implemented an ultrasonic biotelemetry system to monitor the in situ swimming behavior of L. crocea across varying current velocities and tidal phases. The results indicated that the tagged fish predominantly occupied water depths of 1 to 2.6 m, with no observable circular swimming behavior along the cage periphery. Additionally, the spatial distribution of L. crocea within the large-scale cage seemed to correlate with the direction of the current. Furthermore, both the frequency of appearance and swimming speed of L. crocea were higher in the center of the cage compared to the peripheral regions during flood and ebb tides, whereas the opposite trend was observed during slack water. This study provides novel insights into the behavioral ecology of L. crocea in large-scale aquaculture systems. Full article

To maximize the limited space of aquaculture vessels and achieve a more efficient layout for aquaculture compartments at the bow and stern, this study proposes two design schemes: modifying the compartment configuration and removing the deflector baffle. The study focused on the impact of compartment configuration and fish movement on the flow field characteristics under the two proposed schemes. The results showed that the mean flow velocity in the octagonal tanks was higher at jet angles of 30° and 45°, with the trend index ($\gamma$) being more stable at 30°. Within jet angles ranging from 0° to 45°, the mean flow velocity increased with rising jet velocity. Retaining the deflector baffle helped stabilize the flow field, making it more effective than scheme A, which, in turn, outperformed scheme B. In circular tanks, the mean flow velocity was higher at 0° and 15°, with the trend index being more stable at 0°. Retaining the deflector baffle at low jet angles further stabilized the flow field. Retaining the deflector baffle at low jet angles helped maintain high average flow velocity, while at high jet angles, it reduced turbulence. Therefore, scheme A demonstrated a better balance between aquaculture volume and flow field stability. Fish simulation experiments revealed that the presence of fish significantly hinders fluid flow and disrupts the stability of the flow field. In practical aquaculture, the jet angle and the use of deflector baffles should be selected based on the tank geometry, while the jet velocity should be adjusted according to the fish species and their swimming speed to establish a flow environment conducive to fish survival and growth. Full article

The Lima River in northern Portugal serves as a vital habitat for diadromous fish species, yet it remains one of the least studied aquatic systems in the region. This study investigates the ecology and biology of key diadromous fish populations, including sea lamprey (Petromyzon marinus), shads (Alosa alosa and Alosa fallax), trout (Salmo trutta), and European eel (Anguilla anguilla), during their reproductive migration and riverine periods. A total of 3242 individuals from 15 species were sampled, with native species accounting for 51.1% of the catch. Results revealed significant differences in size and weight of lampreys, with individuals from the Lima River being significantly smaller than those from regional rivers such as the Minho and Mondego. Shad populations showed a high proportion of hybrids (33.8%), suggesting extensive hybridization between A. alosa and A. fallax. Analysis of trout stomach contents revealed a diverse diet dominated by insects (66.9%), crustaceans (6.8%), and fish (6.8%), but also an alarming presence of plastic debris (1.1%), highlighting potential pollution problems. For European eels, this study marks the first record of the invasive swim bladder parasite Anguillicola crassus in the Lima River, with 84.8% of eels sampled showing moderate to severe swim bladder damage. These findings contribute to a deeper understanding of diadromous fish ecology and emphasize the importance of conservation efforts in estuarine systems worldwide. Full article