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19 pages, 895 KiB  
Article
A Phytochemical and Biological Characterization of Cynara cardunculus L. subsp. scolymus Cultivar “Carciofo di Procida”, a Traditional Italian Agri-Food Product (PAT) of the Campania Region
by Giuseppina Tommonaro, Giulia De Simone, Carmine Iodice, Marco Allarà and Adele Cutignano
Molecules 2025, 30(15), 3285; https://doi.org/10.3390/molecules30153285 (registering DOI) - 5 Aug 2025
Abstract
The artichoke (Cynara cardunculus L. subsp. scolymus) is an endemic perennial plant of the Mediterranean area commonly consumed as food. It is known since ancient times for its beneficial properties for human health, among which its antioxidant activity due to polyphenolics [...] Read more.
The artichoke (Cynara cardunculus L. subsp. scolymus) is an endemic perennial plant of the Mediterranean area commonly consumed as food. It is known since ancient times for its beneficial properties for human health, among which its antioxidant activity due to polyphenolics stands out. In the frame of our ongoing studies aiming to highlight the biodiversity and the chemodiversity of natural resources, we investigated the phenolic and saponin content of the cultivar “Carciofo di Procida” collected at Procida, an island of the Gulf of Naples (Italy). Along with the edible part of the immature flower, we included in our analyses the stem and the external bracts, generally discarded for food consuming or industrial preparations. The LCMS quali-quantitative profiling of polyphenols (including anthocyanins) and cynarasaponins of this cultivar is reported for the first time. In addition to antioxidant properties, we observed a significant cytotoxic activity due to extracts from external bracts against human neuroblastoma SH-SY5Y cell lines with 43% of cell viability, after 24 h from the treatment (50 μg/mL), and less potent but appreciable effects also against human colorectal adenocarcinoma CaCo-2 cells. This suggests that the different metabolite composition may be responsible for the bioactivity of extracts obtained from specific parts of artichoke and foresees a possible exploitation of the discarded material as a source of beneficial compounds. Full article
(This article belongs to the Special Issue Extraction and Analysis of Natural Products in Food—3rd Edition)
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13 pages, 988 KiB  
Article
Assessing the Applicability of a Partial Alcohol Reduction Method to the Fine Wine Analytical Composition of Pinot Gris
by Diána Ágnes Nyitrainé Sárdy, Péter Bodor-Pesti and Szabina Steckl
Foods 2025, 14(15), 2738; https://doi.org/10.3390/foods14152738 - 5 Aug 2025
Abstract
Climate change has a significant negative impact on agriculture and food production. This trend requires technological development and the adaptation of new technologies in both the grapevine production and winemaking sectors. High temperatures and heat accumulation during the growing season result in faster [...] Read more.
Climate change has a significant negative impact on agriculture and food production. This trend requires technological development and the adaptation of new technologies in both the grapevine production and winemaking sectors. High temperatures and heat accumulation during the growing season result in faster ripening and a higher sugar content, leading to a higher alcohol content during fermentation. The negative consequences are an imbalanced wine character and consumer reluctance, as lower alcoholic beverages are now in high demand. Over the last decade, several methods have been developed to handle this impact and reduce the alcohol content of wines. In this study, we used the MASTERMIND® REMOVE membrane-based dealcoholization system to reduce the alcohol concentration in of Pinot gris wines from 12.02% v/v to 10.69% v/v and to investigate the effect on analytical parameters in three steps (0.5%, 1%, and 1.5% reductions) along the treatment. To evaluate the impact of the partial alcohol reduction and identify correlations between the wine chemical parameters, data were analyzed with ANOVA, PCA, multivariate linear regression and cluster analysis. The results showed that except for the extract, sugar content and proline content, the treatment had a significant effect on the chemical parameters. Both free and total SO2 levels were significantly reduced as well as volatile acid, glycerol and succinic acid levels. It must be highlighted that some parameters were not differing significantly between the untreated and the final wine, while the change was statistically verified in the intermediate steps of the partial alcohol reduction. This was the case for example for n-Propanol, i-Amylalcohol, Acetaldehyde, and Ethyl acetate. The multivariate linear regression model explained 18.84% of the total variance, indicating a modest but meaningful relationship between the alcohol content and the investigated analytical parameters. Our results showed that even if the applied instrument significantly modified some of the wine chemical parameters, those changes would not influence significantly the wine sensory attributes. Full article
(This article belongs to the Special Issue Winemaking: Innovative Technology and Sensory Analysis)
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30 pages, 3316 KiB  
Systematic Review
Preclinical Evidence of Curcuma longa Linn. as a Functional Food in the Management of Metabolic Syndrome: A Systematic Review and Meta-Analysis of Rodent Studies
by Samuel Abiodun Kehinde, Zahid Naeem Qaisrani, Rinrada Pattanayaiying, Wai Phyo Lin, Bo Bo Lay, Khin Yadanar Phyo, Myat Mon San, Nurulhusna Awaeloh, Sasithon Aunsorn, Ran Kitkangplu and Sasitorn Chusri
Biomedicines 2025, 13(8), 1911; https://doi.org/10.3390/biomedicines13081911 - 5 Aug 2025
Abstract
Background/Objectives: Metabolic syndrome (MetS) is a multifactorial condition characterized by abdominal obesity, dyslipidemia, insulin resistance, hypertension, and chronic inflammation. As its global prevalence rises, there is increasing interest in natural, multi-targeted approaches to manage MetS. Curcuma longa Linn. (turmeric), especially its active [...] Read more.
Background/Objectives: Metabolic syndrome (MetS) is a multifactorial condition characterized by abdominal obesity, dyslipidemia, insulin resistance, hypertension, and chronic inflammation. As its global prevalence rises, there is increasing interest in natural, multi-targeted approaches to manage MetS. Curcuma longa Linn. (turmeric), especially its active compound curcumin, has shown therapeutic promise in preclinical studies. This systematic review and meta-analysis evaluated the effects of Curcuma longa and its derivatives on MetS-related outcomes in rodent models. Methods: A comprehensive search was conducted across six databases (PubMed, Scopus, AMED, LILACS, MDPI, and Google Scholar), yielding 47 eligible in vivo studies. Data were extracted on key metabolic, inflammatory, and oxidative stress markers and analyzed using random-effects models. Results were presented as mean differences (MD) with 95% confidence intervals (CI). Results: Meta-analysis showed that curcumin significantly reduced body weight (rats: MD = −42.10; mice: MD = −2.91), blood glucose (rats: MD = −55.59; mice: MD = −28.69), triglycerides (rats: MD = −70.17; mice: MD = −24.57), total cholesterol (rats: MD = −35.77; mice: MD = −52.61), and LDL cholesterol (rats: MD = −69.34; mice: MD = −42.93). HDL cholesterol increased significantly in rats but not in mice. Inflammatory cytokines were markedly reduced, while oxidative stress improved via decreased malondialdehyde (MDA) and elevated superoxide dismutase (SOD) and catalase (CAT) levels. Heterogeneity was moderate to high, primarily due to variations in curcumin dosage (ranging from 10 to 500 mg/kg) and treatment duration (2 to 16 weeks) across studies. Conclusions: This preclinical evidence supports Curcuma longa as a promising functional food component for preventing and managing MetS. Its multi-faceted effects warrant further clinical studies to validate its translational potential. Full article
(This article belongs to the Special Issue The Role of Cytokines in Health and Disease: 3rd Edition)
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11 pages, 1037 KiB  
Article
Bonding to Demineralized Dentin: Impact of Immediate and Delayed Dentin Sealing over Time
by Erika Pérez-Soto, Rim Bourgi, Louis Hardan, Carlos Enrique Cuevas-Suarez, Ana Josefina Monjáras-Ávila, Miguel Ángel Fernández-Barrera, Nicolas Nassar, Monika Lukomska-Szymanska, Rima Daoui, Naji Kharouf and Youssef Haikel
Dent. J. 2025, 13(8), 354; https://doi.org/10.3390/dj13080354 - 5 Aug 2025
Abstract
Background/Objectives: Immediate dentin sealing (IDS) has been widely investigated in sound dentin; however, its efficacy on demineralized dentin remains insufficiently explored. This in vitro experimental study aimed to evaluate the shear bond strength (SBS) of indirect composite resin restorations bonded to demineralized dentin [...] Read more.
Background/Objectives: Immediate dentin sealing (IDS) has been widely investigated in sound dentin; however, its efficacy on demineralized dentin remains insufficiently explored. This in vitro experimental study aimed to evaluate the shear bond strength (SBS) of indirect composite resin restorations bonded to demineralized dentin using IDS, assessed at 24 h and after 6 months of aging. Methods: Twenty-five extracted premolars were randomly divided into five groups: (1) control (no sealing), (2) IDS applied to sound dentin (sound-IDS), (3) IDS applied to demineralized dentin (carious-IDS), (4) delayed dentin sealing (DDS) on sound dentin (sound-DDS), and (5) DDS on demineralized dentin (carious-DDS). SBS values were analyzed using a three-way analysis of variance (ANOVA) with dentin condition (sound vs. demineralized), aging time (24 h vs. 6 months), and sealing strategy (control, IDS, DDS) as independent variables. Statistical analyses were performed using SigmaPlot 12.0, with significance set at p < 0.05. Results: The results showed that IDS led to significantly higher SBS than DDS (p < 0.05). Bond strength was significantly influenced by dentin condition (p < 0.05), and all interactions between variables—particularly between dentin condition and sealing strategy, and between aging time and treatment—were statistically significant (p < 0.001). Overall, bond strength was higher at 24 h than after 6 months. IDS showed optimal performance in sound dentin, while DDS resulted in better long-term outcomes in demineralized dentin. Conclusions: These findings suggest that DDS may be the more effective approach in cases of carious or demineralized dentin. Full article
(This article belongs to the Section Restorative Dentistry and Traumatology)
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19 pages, 1492 KiB  
Review
Ginseng Nanosizing: The Second Spring of Ginseng Therapeutic Applications
by Jian Wang, Huan Liu, Xinshuo Ding, Tianqi Liu, Qianyuan Li, Runyuan Li, Yuan Yuan, Xiaoyu Yan and Jing Su
Antioxidants 2025, 14(8), 961; https://doi.org/10.3390/antiox14080961 (registering DOI) - 5 Aug 2025
Abstract
Plant-derived vesicles offer several advantages, including high yield, low cost, ethical compatibility, safety, and potential health benefits. These advantages enable them to overcome technological limitations associated with vesicles of mammalian origin. Ginseng, a prominent example of a natural botanical plant, is known for [...] Read more.
Plant-derived vesicles offer several advantages, including high yield, low cost, ethical compatibility, safety, and potential health benefits. These advantages enable them to overcome technological limitations associated with vesicles of mammalian origin. Ginseng, a prominent example of a natural botanical plant, is known for its abundant bioactive components. Recent studies confirmed that ginseng-derived vesicles offer significant advantages in the treatment of human diseases. Therefore, this study reviews the extraction and purification processes of ginseng-derived vesicle-like nanoparticles (GDVLNs), their therapeutic potential, and the active ingredients in GDVLNs that may exert pharmacological activities. Furthermore, this study evaluates the research and applications of nanosized ginseng extracts, with a primary focus on ginsenosides. Full article
(This article belongs to the Special Issue Antioxidant and Protective Effects of Plant Extracts—2nd Edition)
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16 pages, 1991 KiB  
Article
Antihypertensive Effects of Lotus Seed (Nelumbo nucifera Gaertn.) Extract via eNOS Upregulation and Oxidative Stress Reduction in L-NAME-Induced Hypertensive Rats
by Anjaree Inchan, Tippaporn Bualeong, Worasak Kaewkong, Nitra Nuengchamnong, Phapada Apaikawee, Pakaporn Sa-Nguanpong, Wiriyaporn Sumsakul, Natthawut Charoenphon, Usana Chatturong, Watcharakorn Deetud and Krongkarn Chootip
Pharmaceuticals 2025, 18(8), 1156; https://doi.org/10.3390/ph18081156 - 4 Aug 2025
Abstract
Background/Objectives: Nelumbo nucifera Gaertn. (lotus) seeds have traditionally been used to treat hypertension, though their mechanisms remain unclear. This study investigated the antihypertensive effects of lotus seed extract (LSE) and its mechanisms in rats with Nω-nitro-L-arginine methyl ester (L-NAME)-induced hypertension. [...] Read more.
Background/Objectives: Nelumbo nucifera Gaertn. (lotus) seeds have traditionally been used to treat hypertension, though their mechanisms remain unclear. This study investigated the antihypertensive effects of lotus seed extract (LSE) and its mechanisms in rats with Nω-nitro-L-arginine methyl ester (L-NAME)-induced hypertension. Methods: Male Sprague Dawley rats received L-NAME (40 mg/kg/day) in drinking water and were treated orally with LSE (5, 10, or 100 mg/kg/day), captopril (5 mg/kg/day), or a combination of LSE and captopril (2.5 mg/kg/day each) for 5 weeks. Hemodynamic parameters and histological changes in the left ventricle and aorta were assessed. Mechanistic studies included measurements of plasma nitric oxide (NO) metabolites, malondialdehyde (MDA), superoxide dismutase (SOD) activity, angiotensin II (Ang II), angiotensin-converting enzyme (ACE) activity, and protein expression via western blot. Results: L-NAME elevated systolic blood pressure and induced cardiovascular remodeling, oxidative stress, and renin-angiotensin system activation. LSE treatment reduced blood pressure, improved antioxidant status, increased NO bioavailability, and downregulated gp91phox and AT1R expression. The combination of low-dose LSE and captopril produced stronger effects than LSE alone, with efficacy comparable to captopril. Conclusions: These findings suggest that LSE exerts antihypertensive effects via antioxidant activity and inhibition of the renin-angiotensin system, supporting its potential as an adjunct therapy for hypertension. Full article
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15 pages, 642 KiB  
Article
Evaluation of the Profile of Selected Bioactive Compounds and the Potential of Barley Wort Enriched with Malted and Unmalted Hemp Seeds for Brewing Applications
by Marek Zdaniewicz, Robert Duliński, Jana Lakatošová, Janusz Gołaszewski and Krystyna Żuk-Gołaszewska
Molecules 2025, 30(15), 3261; https://doi.org/10.3390/molecules30153261 - 4 Aug 2025
Abstract
The incorporation of Cannabis sativa L. seeds into barley wort was investigated to enhance the functional profile of beer. Hemp seeds (cv. Henola) were malted via controlled steeping, germination, and kilning, then added to barley malt at 10% and 30% (w/ [...] Read more.
The incorporation of Cannabis sativa L. seeds into barley wort was investigated to enhance the functional profile of beer. Hemp seeds (cv. Henola) were malted via controlled steeping, germination, and kilning, then added to barley malt at 10% and 30% (w/w) in both malted and unmalted forms. Standard congress mashing produced worts whose physicochemical parameters (pH, extract, colour, turbidity, filtration and saccharification times) were assessed, alongside profiles of fermentable sugars, polyphenols, B-group vitamins, and cannabinoids. Addition of hemp seeds reduced extract yield without impairing saccharification or filtration and slightly elevated mash pH and turbidity. Maltose and glucose levels declined significantly at higher hemp dosages, whereas sucrose remained stable. Wort enriched with 30% unmalted seeds exhibited the highest levels of trans-ferulic (20.61 µg/g), gallic (5.66 µg/g), trans-p-coumaric (3.68 µg/g), quercetin (6.07 µg/g), and trans-cinnamic (4.07 µg/g) acids. Malted hemp addition enhanced thiamine (up to 0.302 mg/mL) and riboflavin (up to 178.8 µg/mL) concentrations. Cannabinoids (THCA-A, THCV, CBDV, CBG, CBN) were successfully extracted at µg/mL levels, with the total cannabinoid content peaking at 14.59 µg/mL in the 30% malted treatment. These findings demonstrate that hemp seeds, particularly in malted form, can enrich barley wort with bioactive polyphenols, vitamins, and non-psychoactive cannabinoids under standard mashing conditions, without compromising key brewing performance metrics. Further work on fermentation, sensory evaluation, stability, and bioavailability is warranted to realise hemp-enriched functional beers. Full article
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23 pages, 3877 KiB  
Article
Enhancing Bioactive Compound Extraction from Rose Hips Using Pulsed Electric Field (PEF) Treatment: Impacts on Polyphenols, Carotenoids, Volatiles, and Fermentation Potential
by George Ntourtoglou, Chaido Bardouki, Andreas Douros, Nikolaos Gkanatsios, Eleni Bozinou, Vassilis Athanasiadis, Stavros I. Lalas and Vassilis G. Dourtoglou
Molecules 2025, 30(15), 3259; https://doi.org/10.3390/molecules30153259 - 4 Aug 2025
Abstract
Rose hips are rich in polyphenols, making them a promising ingredient for the development of functional fruit-based beverages. This study aimed to evaluate the effect of Pulsed Electric Field (PEF) extraction treatment on rose hip (RH) pulp to enhance the extraction of polyphenols, [...] Read more.
Rose hips are rich in polyphenols, making them a promising ingredient for the development of functional fruit-based beverages. This study aimed to evaluate the effect of Pulsed Electric Field (PEF) extraction treatment on rose hip (RH) pulp to enhance the extraction of polyphenols, carotenoids, and volatile compounds. Additionally, this study examined the impact of adding rose hip berries during different stages of carbohydrate fermentation on the resulting phenolic and aroma profiles. A control wort and four experimental formulations were prepared. Rose hip pulp—treated or untreated with PEF—was added either during fermentation or beforehand, and the volatiles produced were analyzed using GC-MS (in triplicate). Fermentation was carried out over 10 days at 20 °C using Saccharomyces cerevisiae and Torulaspora delbrueckii. At a 10:1 ratio, all beverage samples were subjected to physicochemical testing and HPLC analysis for polyphenols, organic acids, and carotenoids, as well as GC-MS analysis for aroma compounds. The results demonstrated that the use of PEF-treated rose hips significantly improved phenolic compound extraction. Moreover, the PEF treatment enhanced the aroma profile of the beverage, contributing to a more complex and appealing sensory experience. This research highlights the rich polyphenol content of rose hips and the potential of PEF-treated fruit as a natural ingredient to improve both the functional and sensory qualities of fruit-based beverages. Their application opens new possibilities for the development of innovative, health-promoting drinks in the brewing industry. Full article
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23 pages, 2295 KiB  
Review
Advances in Interfacial Engineering and Structural Optimization for Diamond Schottky Barrier Diodes
by Shihao Lu, Xufang Zhang, Shichao Wang, Mingkun Li, Shuopei Jiao, Yuesong Liang, Wei Wang and Jing Zhang
Materials 2025, 18(15), 3657; https://doi.org/10.3390/ma18153657 - 4 Aug 2025
Abstract
Diamond, renowned for its exceptional electrical, physical, and chemical properties, including ultra-wide bandgap, superior hardness, high thermal conductivity, and unparalleled stability, serves as an ideal candidate for next-generation high-power and high-temperature electronic devices. Among diamond-based devices, Schottky barrier diodes (SBDs) have garnered significant [...] Read more.
Diamond, renowned for its exceptional electrical, physical, and chemical properties, including ultra-wide bandgap, superior hardness, high thermal conductivity, and unparalleled stability, serves as an ideal candidate for next-generation high-power and high-temperature electronic devices. Among diamond-based devices, Schottky barrier diodes (SBDs) have garnered significant attention due to their simple architecture and superior rectifying characteristics. This review systematically summarizes recent advances in diamond SBDs, focusing on both metal–semiconductor (MS) and metal–interlayer–semiconductor (MIS) configurations. For MS structures, we critically analyze the roles of single-layer metals (including noble metals, transition metals, and other metals) and multilayer metals in modulating Schottky barrier height (SBH) and enhancing thermal stability. However, the presence of interface-related issues such as high densities of surface states and Fermi level pinning often leads to poor control of the SBH, limiting device performance and reliability. To address these challenges and achieve high-quality metal/diamond interfaces, researchers have proposed various interface engineering strategies. In particular, the introduction of interfacial layers in MIS structures has emerged as a promising approach. For MIS architectures, functional interlayers—including high-k materials (Al2O3, HfO2, SnO2) and low-work-function materials (LaB6, CeB6)—are evaluated for their efficacy in interface passivation, barrier modulation, and electric field control. Terminal engineering strategies, such as field-plate designs and surface termination treatments, are also highlighted for their role in improving breakdown voltage. Furthermore, we emphasize the limitations in current parameter extraction from current–voltage (I–V) properties and call for a unified new method to accurately determine SBH. This comprehensive analysis provides critical insights into interface engineering strategies and evaluation protocols for high-performance diamond SBDs, paving the way for their reliable deployment in extreme conditions. Full article
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23 pages, 2688 KiB  
Article
Effect of Biostimulant Applications on Eco-Physiological Traits, Yield, and Fruit Quality of Two Raspberry Cultivars
by Francesco Giovanelli, Cristian Silvestri and Valerio Cristofori
Horticulturae 2025, 11(8), 906; https://doi.org/10.3390/horticulturae11080906 (registering DOI) - 4 Aug 2025
Abstract
Enhancing the yield and qualitative traits of horticultural crops without further hampering the environment constitutes an urgent challenge that could be addressed by implementing innovative agronomic tools, such as plant biostimulants. This study investigated the effects of three commercial biostimulants—BIO1 (fulvic/humic acids), BIO2 [...] Read more.
Enhancing the yield and qualitative traits of horticultural crops without further hampering the environment constitutes an urgent challenge that could be addressed by implementing innovative agronomic tools, such as plant biostimulants. This study investigated the effects of three commercial biostimulants—BIO1 (fulvic/humic acids), BIO2 (leonardite-humic acids), and BIO3 (plant-based extracts)—on leaf ecophysiology, yield, and fruit quality in two raspberry cultivars, ‘Autumn Bliss’ (AB) and ‘Zeva’ (Z), grown in an open-field context, to assess their effectiveness in raspberry cultivation. Experimental activities involved two Research Years (RYs), namely, year 2023 (RY 1) and 2024 (RY 2). Leaf parameters such as chlorophyll, flavonols, anthocyanins, and the Nitrogen Balance Index (NBI) were predominantly influenced by the interaction between Treatment, Year and Cultivar factors, indicating context-dependent responses rather than direct biostimulant effects. BIO2 showed a tendency to increase yield (g plant−1) and berry number plant−1, particularly in RY 2 (417.50 g plant−1, +33.93% vs. control). Fruit quality responses were cultivar and time-specific: BIO3 improved soluble solid content in AB (12.8 °Brix, RY 2, Intermediate Harvest) and Z (11.43 °Brix, +13.91% vs. BIO2). BIO2 reduced titratable acidity in AB (3.12 g L−1) and increased pH in Z (3.02, RY 2) but also decreased °Brix in Z. These findings highlight the potential of biostimulants to modulate raspberry physiology and productivity but underscore the critical role of cultivar, environmental conditions, and specific biostimulant composition in determining the outcomes, which were found to critically depend on tailored application strategies. Full article
(This article belongs to the Section Fruit Production Systems)
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18 pages, 1256 KiB  
Article
Algae Extracts and Zeolite Modulate Plant Growth and Enhance the Yield of Tomato Solanum lycopersicum L. Under Suboptimum and Deficient Soil Water Content
by José Antonio Miranda-Rojas, Aurelio Pedroza-Sandoval, Isaac Gramillo-Ávila, Ricardo Trejo-Calzada, Ignacio Sánchez-Cohen and Luis Gerardo Yáñez-Chávez
Horticulturae 2025, 11(8), 902; https://doi.org/10.3390/horticulturae11080902 (registering DOI) - 3 Aug 2025
Abstract
Drought and water scarcity are some of the most important challenges facing agricultural producers in dry environments. This study aimed to evaluate the effect of algae extract and zeolite in terms of their biostimulant action on water stress tolerance to obtain better growth [...] Read more.
Drought and water scarcity are some of the most important challenges facing agricultural producers in dry environments. This study aimed to evaluate the effect of algae extract and zeolite in terms of their biostimulant action on water stress tolerance to obtain better growth and production of tomato Lycopersicum esculentum L. grown in an open field under suboptimum and deficient soil moisture content. Large plots had a suboptimum soil moisture content (SSMC) of 25% ± 2 [28% below field capacity (FC)] and deficient soil moisture content (DSMC) of 20% ± 2 [11% above permanent wilting point (PWP)]; both soil moisture ranges were based on field capacity FC (32%) and PWP (18%). Small plots had four treatments: algae extract (AE) 50 L ha−1 and zeolite (Z) 20 t ha−1, a combination of both products (AE + Z) 25 L ha−1 and 10 t h−1, and a control (without application of either product). By applying AE, Z, and AE + Z, plant height, plant vigor, and chlorophyll index were significantly higher compared to the control by 20.3%, 10.5%, and 22.3%, respectively. The effect on relative water content was moderate—only 2.6% higher than the control applying AE, while the best treatment for the photosynthesis variable was applying Z, with a value of 20.9 μmol CO2 m−2 s−1, which was 18% higher than the control. Consequently, tomato yield was also higher compared to the control by 333% and 425% when applying AE and Z, respectively, with suboptimum soil moisture content. The application of the biostimulants did not show any mitigating effect on water stress under soil water deficit conditions close to permanent wilting. These findings are relevant to water-scarce agricultural areas, where more efficient irrigation water use is imperative. Plant biostimulation through organic and inorganic extracts plays an important role in mitigating environmental stresses such as those caused by water shortages, leading to improved production in vulnerable agricultural areas with extreme climates. Full article
(This article belongs to the Special Issue Optimized Irrigation and Water Management in Horticultural Production)
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16 pages, 3226 KiB  
Article
Sustainable Agronomical Practices Affect Essential Oil Composition of Tanacetum balsamita L.
by Martina Grattacaso, Alessandra Bonetti, Sara Di Lonardo and Luigi Paolo D’Acqui
Plants 2025, 14(15), 2406; https://doi.org/10.3390/plants14152406 - 3 Aug 2025
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Abstract
This study evaluated the influence of compost and bioinoculants (mycorrhizal fungi and plant growth-promoting bacteria) on the yield and composition of essential oil extracted from Tanacetum balsamita L. over two growing seasons. The plants were cultivated under four treatments: compost, bioinoculants, a combination [...] Read more.
This study evaluated the influence of compost and bioinoculants (mycorrhizal fungi and plant growth-promoting bacteria) on the yield and composition of essential oil extracted from Tanacetum balsamita L. over two growing seasons. The plants were cultivated under four treatments: compost, bioinoculants, a combination (bioinoculants + compost), and a control. At each harvest, essential oil was extracted from fresh leaves via stem-flow distillation and analyzed using gas chromatography coupled with single quadrupole mass spectrometry. Twenty to twenty-four compounds were identified. Based on the dominant terpene derivative, the results indicated that Tanacetum balsamita L. cultivated in Italy belongs to “camphor” chemotype, a pharmacologically active compound known for its antimicrobial, anti-inflammatory, and analgesic properties. Moreover, three compounds, α-, β-phellandrene and myrtenol, were identified as typical of Tanacetum balsamita L. cultivated in Italy. Treatment effects were significant for some compounds (camphor, borneol, terpinen-4-ol, α-terpineol, dehydro sabinene ketone, and 3-thujanol), and the interaction between treatment and year was significant for a few compounds (borneol, terpinen-4-ol, dehydro sabinene ketone, 1,8-cineol, and 3-thujanol). These results emphasize the need to account for seasonal variation and underline the necessity of a deeper understanding of how experimental factors interact with them, especially in long-term essential oil studies. Full article
(This article belongs to the Special Issue Chemical Analysis, Bioactivity, and Application of Essential Oils)
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18 pages, 10504 KiB  
Article
Green Synthesis and Characterization of Silver Nanoparticles Using Artemisia terrae-albae Extracts and Evaluation of Their Cytogenotoxic Effects
by Moldyr Dyusebaeva, Dmitriy Berillo, Zhansaya Yesbussinova, Nailya Ibragimova, Daniil Shepilov, Sandugash Sydykbayeva, Almagul Almabekova, Nurzhan Chinibayeva, Adewale Olufunsho Adeloye and Gulzat Berganayeva
Int. J. Mol. Sci. 2025, 26(15), 7499; https://doi.org/10.3390/ijms26157499 (registering DOI) - 3 Aug 2025
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Abstract
The development of non-toxic silver nanoparticles (AgNPs) for medical and other diverse applications is steadily increasing. However, this study specifically aims to determine the cytotoxic effects of AgNPs synthesized via a green chemistry approach using aqueous-ethanol and ethyl acetate extracts of Artemisia terrae-albae [...] Read more.
The development of non-toxic silver nanoparticles (AgNPs) for medical and other diverse applications is steadily increasing. However, this study specifically aims to determine the cytotoxic effects of AgNPs synthesized via a green chemistry approach using aqueous-ethanol and ethyl acetate extracts of Artemisia terrae-albae. The photophysical, morphological, and size distribution characteristics of the synthesized AgNPs are analyzed using UV-Vis spectroscopy and transmission electron microscopy (TEM). A modified Allium cepa assay is employed to evaluate biological responses, including root growth, root number, and mitotic index. In this assay, the cell cycles of onion bulbs are synchronized and pre-incubated at 4 °C for 72 h prior to treatment. This study reveals that the AgNPs synthesized from the ethanol extract exhibit notable stability and higher cytotoxicity activity, with a root length of 0.6 ± 0.13 cm, root number of 16 ± 6.88, and mitotic index of 25.0 ± 2.6. These values are significantly more cytogenotoxic than those observed for the ethyl-acetate-derived nanoparticles, which show a root length of 0.8 ± 0.17 cm, root number of 18 ± 6.27, and mitotic index of 36 ± 3.6. These findings highlight the potential of green-synthesized AgNPs as effective cytotoxic agents, especially those obtained from ethanol extract, possibly due to a greater influence of the quantity of diverse phenolic compounds present in the complex mixtures than in the ethyl acetate extract, which otherwise enhanced their morphology, shape, and size. These, overall, contributed to the biological activity. Full article
(This article belongs to the Special Issue Latest Advances in Nanoparticles for Modern Biomedicine (2nd Edition))
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17 pages, 5214 KiB  
Article
Geothermal–Peltier Hybrid System for Air Cooling and Water Recovery
by Michele Spagnolo, Paolo Maria Congedo, Alessandro Buscemi, Gianluca Falcicchia Ferrara, Marina Bonomolo and Cristina Baglivo
Energies 2025, 18(15), 4115; https://doi.org/10.3390/en18154115 - 3 Aug 2025
Viewed by 71
Abstract
This study proposes a new air treatment system that integrates dehumidification, cooling, and water recovery using a Horizontal Air–Ground Heat Exchanger (HAGHE) combined with Peltier cells. The airflow generated by a fan flows through an HAGHE until it meets a septum on which [...] Read more.
This study proposes a new air treatment system that integrates dehumidification, cooling, and water recovery using a Horizontal Air–Ground Heat Exchanger (HAGHE) combined with Peltier cells. The airflow generated by a fan flows through an HAGHE until it meets a septum on which Peltier cells are placed, and then separates into two distinct streams that lap the two surfaces of the Peltier cells: one stream passes through the cold surfaces, undergoing both sensible and latent cooling with dehumidification; the other stream passes through the hot surfaces, increasing its temperature. The two treated air streams may then pass through a mixing chamber, where they are combined in the appropriate proportions to achieve the desired air supply conditions and ensure thermal comfort in the indoor environment. A Computational Fluid Dynamics (CFD) analysis was carried out to simulate the thermal interaction between the HAGHE and the surrounding soil. The simulation focused on a system installed under the subtropical climate conditions of Nairobi, Africa. The simulation results demonstrate that the HAGHE system is capable of reducing the air temperature by several degrees under typical summer conditions, with enhanced performance observed when the soil is moist. Condensation phenomena were triggered when the relative humidity of the inlet air exceeded 60%, contributing additional cooling through latent heat extraction. The proposed HAGHE–Peltier system can be easily powered by renewable energy sources and configured for stand-alone operation, making it particularly suitable for off-grid applications. Full article
(This article belongs to the Section A: Sustainable Energy)
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15 pages, 2885 KiB  
Article
Effects of Modified Senna obtusifolia Straw Biochar on Organic Matter Mineralization and Nutrient Transformation in Siraitia grosvenorii Farmland
by Lening Hu, Yinnan Bai, Shu Li, Gaoyan Liu, Jingxiao Liang, Hua Deng, Anyu Li, Linxuan Li, Limei Pan and Yuan Huang
Agronomy 2025, 15(8), 1877; https://doi.org/10.3390/agronomy15081877 - 3 Aug 2025
Viewed by 114
Abstract
Biochar has garnered considerable attention as a soil amendment due to its unique physicochemical properties. Its application not only enhances soil carbon sequestration but also improves nutrient availability. Incorporating biochar into soil is regarded as a promising strategy for mitigating global climate change [...] Read more.
Biochar has garnered considerable attention as a soil amendment due to its unique physicochemical properties. Its application not only enhances soil carbon sequestration but also improves nutrient availability. Incorporating biochar into soil is regarded as a promising strategy for mitigating global climate change while delivering substantial environmental and agricultural benefits. In this study, biochar was extracted from Siraitia grosvenorii and subsequently modified through alkali treatment. A laboratory incubation experiment was conducted to assess the effects of unmodified (JMC) and modified (GXC) biochar, applied at different rates (1%, 2%, and 4%), on organic carbon mineralization and soil nutrient dynamics. Results indicated that, at equivalent application rates, JMC-treated soils exhibited lower CO2 emissions than those treated with GXC, with emissions increasing alongside biochar dosage. After the incubation, the 1% JMC treatment exhibited a mineralization rate of 17.3 mg·kg−1·d−1, which was lower than that of the control (CK, 18.8 mg·kg−1·d−1), suggesting that JMC effectively inhibited organic carbon mineralization and reduced CO2 emissions, thereby contributing positively to carbon sequestration in Siraitia grosvenorii farmland. In contrast, GXC application significantly enhanced soil nutrient levels, particularly increasing available phosphorus (AP) by 14.33% to 157.99%. Furthermore, partial least squares structural equation modeling (PLS-SEM) identified application rate and pH as the key direct factors influencing soil nutrient availability. Full article
(This article belongs to the Section Soil and Plant Nutrition)
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