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32 pages, 12213 KiB  
Review
Capacitive Sensors for Label-Free Detection in High-Ionic-Strength Bodily Fluids: A Review
by Seerat Sekhon, Richard Bayford and Andreas Demosthenous
Biosensors 2025, 15(8), 491; https://doi.org/10.3390/bios15080491 - 30 Jul 2025
Viewed by 316
Abstract
Capacitive sensors are platforms that enable label-free, real-time detection at low non-perturbing voltages. These sensors do not rely on Faradaic processes, thereby eliminating the need for redox-active species and simplifying system integration for point-of-care diagnostics. However, their sensitivity in high-ionic-strength solutions, such as [...] Read more.
Capacitive sensors are platforms that enable label-free, real-time detection at low non-perturbing voltages. These sensors do not rely on Faradaic processes, thereby eliminating the need for redox-active species and simplifying system integration for point-of-care diagnostics. However, their sensitivity in high-ionic-strength solutions, such as bodily fluids, is limited due to a reduced Debye length and non-specific interactions. The present review highlights advances in material integration, surface modification, and signal enhancement techniques to mitigate the challenges of deploying capacitive sensors in biofluids (sweat, saliva, blood, serum). This work further expands on the promise of such sensors for advancing liquid biopsies and highlights key technical challenges in translating capacitive systems to clinics. Full article
(This article belongs to the Special Issue Novel Designs and Applications for Electrochemical Biosensors)
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18 pages, 3248 KiB  
Article
Electrochemical Nanostructured Aptasensor for Direct Detection of Glycated Hemoglobin
by Luminita Fritea, Cosmin-Mihai Cotrut, Iulian Antoniac, Simona Daniela Cavalu, Luciana Dobjanschi, Angela Antonescu, Liviu Moldovan, Maria Domuta and Florin Banica
Int. J. Mol. Sci. 2025, 26(15), 7140; https://doi.org/10.3390/ijms26157140 - 24 Jul 2025
Viewed by 265
Abstract
Glycated hemoglobin (HbA1c) is an important biomarker applied for the diagnosis, evaluation, and management of diabetes; therefore, its accurate determination is crucial. In this study, an innovative nanoplatform was developed, integrating carbon nanotubes (CNTs) with enhanced hydrophilicity achieved through cyclodextrin (CD) functionalization, and [...] Read more.
Glycated hemoglobin (HbA1c) is an important biomarker applied for the diagnosis, evaluation, and management of diabetes; therefore, its accurate determination is crucial. In this study, an innovative nanoplatform was developed, integrating carbon nanotubes (CNTs) with enhanced hydrophilicity achieved through cyclodextrin (CD) functionalization, and combined with gold nanoparticles (AuNPs) electrochemically deposited onto a screen-printed carbon electrode. The nanomaterials significantly improved the analytical performance of the sensor due to their increased surface area and high electrical conductivity. This nanoplatform was employed as a substrate for the covalent attachment of thiolated ferrocene-labeled HbA1c specific aptamer through Au-S binding. The electrochemical signal of ferrocene was covered by a stronger oxidation peak of Fe2+ from the HbA1c structure, leading to the elaboration of a nanostructured aptasensor capable of the direct detection of HbA1c. The electrochemical aptasensor presented a very wide linear range (0.688–11.5%), an acceptable limit of detection (0.098%), and good selectivity and stability, being successfully applied on real samples. This miniaturized, simple, easy-to-use, and fast-responding aptasensor, requiring only a small sample volume, can be considered as a promising candidate for the efficient on-site determination of HbA1c. Full article
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29 pages, 23821 KiB  
Review
Covalent Organic Frameworks for Immunoassays: A Review
by Suling Yang and Hongmin Liu
Biosensors 2025, 15(7), 469; https://doi.org/10.3390/bios15070469 - 21 Jul 2025
Viewed by 547
Abstract
Immunoassays relying on highly specific antigen–antibody recognition are important tools for effectively measuring the levels of various targets. Efforts have been made in the development of various methods to improve the detection sensitivity and stability of immunoassays. Covalent organic frameworks (COFs), as an [...] Read more.
Immunoassays relying on highly specific antigen–antibody recognition are important tools for effectively measuring the levels of various targets. Efforts have been made in the development of various methods to improve the detection sensitivity and stability of immunoassays. Covalent organic frameworks (COFs), as an emerging class of novel crystalline porous materials, have unique advantages such as flexible designability, high surface area, excellent stability, tunable pore sizes, and multiple functionalities. They have great potential as novel sensory materials. Herein, we summarize the advances of COFs in electrochemical and optical immunoassays serving as electrode modifiers, signal indicators, enzyme or probe carriers, etc. Meanwhile, the design and application of typical COFs-based immunoassays in the determination of different targets are discussed in detail. Finally, challenges and future perspectives are presented. Full article
(This article belongs to the Special Issue Biosensors Based on Self-Assembly and Boronate Affinity Interaction)
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40 pages, 2353 KiB  
Review
Electrochemical Impedance Spectroscopy-Based Biosensors for Label-Free Detection of Pathogens
by Huaiwei Zhang, Zhuang Sun, Kaiqiang Sun, Quanwang Liu, Wubo Chu, Li Fu, Dan Dai, Zhiqiang Liang and Cheng-Te Lin
Biosensors 2025, 15(7), 443; https://doi.org/10.3390/bios15070443 - 10 Jul 2025
Viewed by 641
Abstract
The escalating threat of infectious diseases necessitates the development of diagnostic technologies that are not only rapid and sensitive but also deployable at the point of care. Electrochemical impedance spectroscopy (EIS) has emerged as a leading technique for the label-free detection of pathogens, [...] Read more.
The escalating threat of infectious diseases necessitates the development of diagnostic technologies that are not only rapid and sensitive but also deployable at the point of care. Electrochemical impedance spectroscopy (EIS) has emerged as a leading technique for the label-free detection of pathogens, offering a unique combination of sensitivity, non-invasiveness, and adaptability. This review provides a comprehensive overview of the design and application of EIS-based biosensors tailored for pathogen detection, focusing on critical components such as biorecognition elements, electrode materials, nanomaterial integration, and surface immobilization strategies. Special emphasis is placed on the mechanisms of signal generation under Faradaic and non-Faradaic modes and how these underpin performance characteristics such as the limit of detection, specificity, and response time. The application spectrum spans bacterial, viral, fungal, and parasitic pathogens, with case studies highlighting detection in complex matrices such as blood, saliva, food, and environmental water. Furthermore, integration with microfluidics and point-of-care systems is explored as a pathway toward real-world deployment. Emerging strategies for multiplexed detection and the utilization of novel nanomaterials underscore the dynamic evolution of the field. Key challenges—including non-specific binding, matrix effects, the inherently low ΔRct/decade sensitivity of impedance transduction, and long-term stability—are critically evaluated alongside recent breakthroughs. This synthesis aims to support the future development of robust, scalable, and user-friendly EIS-based pathogen biosensors with the potential to transform diagnostics across healthcare, food safety, and environmental monitoring. Full article
(This article belongs to the Special Issue Material-Based Biosensors and Biosensing Strategies)
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16 pages, 2888 KiB  
Article
Vitamin K Epoxide Reductase Complex (VKORC1) Electrochemical Genosensors: Towards the Identification of 1639 G>A Genetic Polymorphism
by Tiago Barbosa, Stephanie L. Morais, Renato Carvalho, Júlia M. C. S. Magalhães, Valentina F. Domingues, Cristina Delerue-Matos, Hygor Ferreira-Fernandes, Giovanny R. Pinto, Marlene Santos and Maria Fátima Barroso
Chemosensors 2025, 13(7), 248; https://doi.org/10.3390/chemosensors13070248 - 10 Jul 2025
Viewed by 410
Abstract
Anticoagulants, including warfarin, are often administered to patients who are exhibiting early symptoms of thromboembolic episodes or who have already experienced such episodes. However, warfarin has a limited therapeutic index and might cause bleeding and other clinical problems. Warfarin inhibits the vitamin K [...] Read more.
Anticoagulants, including warfarin, are often administered to patients who are exhibiting early symptoms of thromboembolic episodes or who have already experienced such episodes. However, warfarin has a limited therapeutic index and might cause bleeding and other clinical problems. Warfarin inhibits the vitamin K epoxide reductase complex subunit 1 (VKORC1), an enzyme essential for activating vitamin K, in the coagulation cascade. Genetic factors, such as polymorphisms, can change the natural function of VKORC1, causing variations in the medication reaction among individuals. Hence, before prescribing warfarin, the patient’s genetic profile should also be considered. In this study, an electrochemical genosensor capable of detecting the VKORC1 1639 G>A polymorphism was designed and optimized. This analytical approach detects the electric current obtained during the hybridization reaction between two 52 base pair complementary oligonucleotide sequences. Investigating public bioinformatic platforms, two DNA sequences with the A and G single-nucleotide variants were selected and designed. The experimental protocol of the genosensor implied the formation of a bilayer composed of a thiolate DNA and an alkanethiol immobilized onto gold electrodes, as well as the formation of a DNA duplex using a sandwich-format hybridization reaction through a fluorescein labelled DNA signalling probe and the enzymatic amplification of the electrochemical signal, detected by chronoamperometry. A detection limit of 20 pM and a linear range of 0.05–1.00 nM was obtained. A clear differentiation between A/A, G/A and G/G genotypes in biological samples was successfully identified by his novel device. Full article
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23 pages, 3823 KiB  
Review
Electrochemical Strategies for MicroRNA Quantification Leveraging Amplification and Nanomaterials: A Review
by Alexander Hunt and Gymama Slaughter
Chemosensors 2025, 13(7), 242; https://doi.org/10.3390/chemosensors13070242 - 6 Jul 2025
Viewed by 552
Abstract
MicroRNAs (miRNAs) are small, non-coding RNAs that regulate gene expression and have emerged as critical biomarkers in various diseases, including cancer. Their stability in bodily fluids and role as oncogenes or tumor suppressors make them attractive targets for non-invasive diagnostics. However, conventional detection [...] Read more.
MicroRNAs (miRNAs) are small, non-coding RNAs that regulate gene expression and have emerged as critical biomarkers in various diseases, including cancer. Their stability in bodily fluids and role as oncogenes or tumor suppressors make them attractive targets for non-invasive diagnostics. However, conventional detection methods, such as Northern blotting, RT-PCR, and microarrays, are limited by low sensitivity, lengthy protocols, and limited specificity. Electrochemical biosensors offer a promising alternative, providing high sensitivity, rapid response times, portability, and cost-effectiveness. These biosensors translate miRNA hybridization events into quantifiable electrochemical signals, often leveraging redox-active labels, mediators, or intercalators. Recent advancements in nanomaterials and signal amplification strategies have further enhanced detection capabilities, enabling sensitive, label-free miRNA quantification. This review provides a comprehensive overview of the recent advances in electrochemical biosensing of miRNAs, emphasizing innovative redox-based detection strategies, probe immobilization techniques, and hybridization modalities. The critical challenges and future perspectives in advancing electrochemical miRNA biosensors toward clinical translation and point-of-care diagnostics are discussed. Full article
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15 pages, 6302 KiB  
Article
Fluorescent–Electrochemical–Colorimetric Triple-Model Immunoassays with Multifunctional Metal–Organic Frameworks for Signal Amplification
by Ning Xia, Chuye Zheng and Gang Liu
Biosensors 2025, 15(6), 376; https://doi.org/10.3390/bios15060376 - 11 Jun 2025
Viewed by 598
Abstract
Multimode immunoassays based on multiple response mechanisms have received great attention due to their capacity to effectively improve the accuracy and reliability of biosensing platforms. However, few strategies have been reported for triple-mode immunoassays due to the shortage of multifunctional sensing materials and [...] Read more.
Multimode immunoassays based on multiple response mechanisms have received great attention due to their capacity to effectively improve the accuracy and reliability of biosensing platforms. However, few strategies have been reported for triple-mode immunoassays due to the shortage of multifunctional sensing materials and the incompatibility of signal transduction methods in different detection modes. In this work, a fluorescent–electrochemical–colorimetric triple-mode immunoassay platform was proposed with Cu-based metal–organic frameworks (MOFs) as the signal labels. The captured Cu-MOFs were successfully decomposed under an acidic condition, leading to the release of numerous Cu2+ ions and 2-aminobenzene-1,4-dicarboxylic acid (NH2-BDC) ligands. The released NH2-BDC were determined by fluorescence titration. Meanwhile, the released Cu2+ were readily quantified by differential pulse voltammetry (DPV) and simply detected through the catalytic oxidation of chromogenic substrate 3,3′,5,5′-tetramethylbenzidine (TMB). Taking alpha-fetoprotein (AFP) as a model analyte, the designed triple-mode immunoassays showed good performances with the linear range of 10–200 pg/mL, 10–200 pg/mL, and 1–100 pg/mL for the fluorescent, electrochemical, and colorimetric modes, respectively. The proposed triple-mode biosensing platforms show great potential for the applications in disease diagnosis, since they can be easily extended to other bioassays by changing the targets and recognition elements. Full article
(This article belongs to the Special Issue Signal Amplification in Biosensing)
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11 pages, 1139 KiB  
Article
Electrochemical Sensor Platform for Rapid Detection of Foodborne Toxins
by Kundan Kumar Mishra, Krupa M. Thakkar, Vikram Narayanan Dhamu, Sriram Muthukumar and Shalini Prasad
Biosensors 2025, 15(6), 361; https://doi.org/10.3390/bios15060361 - 4 Jun 2025
Viewed by 719
Abstract
Zearalenone (ZEA), a potent mycotoxin commonly found in contaminated grains, presents a serious threat to food safety and public health. Conventional detection methods, including culture-based assays and laboratory-bound analytical tools, are often time-consuming, require specialized infrastructure, and lack portability, limiting their utility for [...] Read more.
Zearalenone (ZEA), a potent mycotoxin commonly found in contaminated grains, presents a serious threat to food safety and public health. Conventional detection methods, including culture-based assays and laboratory-bound analytical tools, are often time-consuming, require specialized infrastructure, and lack portability, limiting their utility for rapid, on-site screening. In response, this study introduces a compact, real-time electrochemical sensing platform for the swift and selective detection of ZEA in corn flour matrices. Utilizing a non-faradaic, label-free approach based on Electrochemical Impedance Spectroscopy (EIS), the sensor leverages ZEA-specific antibodies to achieve rapid detection within 5 min. The platform demonstrates a low detection limit of 0.05 ng/mL, with a broad dynamic range from 0.1 ng/mL to 25.6 ng/mL. Reproducibility tests confirm consistent performance, with both inter- and intra-assay variation remaining under a 20% coefficient of variation (%CV). Comparative evaluation with standard benchtop systems underscores its accuracy and field applicability. This portable and user-friendly device provides a powerful tool for real-time mycotoxin monitoring, offering significant potential for improving food safety practices and enabling point-of-need testing in resource-limited settings. Full article
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17 pages, 1320 KiB  
Article
Electrochemically Reduced Graphene Oxide Covalently Bound Sensor for Paracetamol Voltammetric Determination
by Amaya Paz de la vega, Fabiana Liendo, Bryan Pichún, Johisner Penagos, Rodrigo Segura and María Jesús Aguirre
Int. J. Mol. Sci. 2025, 26(9), 4267; https://doi.org/10.3390/ijms26094267 - 30 Apr 2025
Viewed by 699
Abstract
Designing a highly sensitive and efficient functionalized electrode for precise drug analysis remains a significant challenge. In this work, an electrochemical sensor based on a glassy carbon electrode (GCE) modified with phenyl diazonium salts (ph) and electrochemically reduced graphene oxide (ERGO), labeled GCE/ph/ERGO, [...] Read more.
Designing a highly sensitive and efficient functionalized electrode for precise drug analysis remains a significant challenge. In this work, an electrochemical sensor based on a glassy carbon electrode (GCE) modified with phenyl diazonium salts (ph) and electrochemically reduced graphene oxide (ERGO), labeled GCE/ph/ERGO, was developed for the detection of paracetamol (PAR) in pharmaceutical matrices using square wave voltammetry (SWV). The modified electrode was characterized by scanning electron microscopy (SEM), electrochemical impedance spectroscopy (EIS), and cyclic voltammetry (CV). Compared to the bare GCE, the GCE/ph/ERGO sensor demonstrated significantly improved conductivity and anodic current peak for PAR over two orders of magnitude higher, indicating a substantial enhancement in electrochemical performance. Under optimized conditions, the developed sensor exhibited a low detection limit of 18.2 nM and a quantification limit of 60.6 nM. Precision studies yielded relative standard deviations (RSDs) below 8%. The sensor demonstrated excellent selectivity in the presence of common pharmaceutical excipients and high accuracy in the analysis of generic pharmaceutical formulations, with results comparable to those obtained by the HPLC technique. These findings confirm the sensor’s reliability, stability, robustness, and suitability for routine analysis of PAR in pharmaceutical samples. Full article
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15 pages, 9862 KiB  
Article
Label-Free Electrochemical Cell-Based Biosensor for Toxicity Assay of Water-Soluble Form of Phosphatidylcholine
by Veronica V. Pronina, Lyubov V. Kostryukova, Sergey V. Ivanov, Elena G. Tichonova, Alexander I. Archakov and Victoria V. Shumyantseva
Biomedicines 2025, 13(4), 996; https://doi.org/10.3390/biomedicines13040996 - 20 Apr 2025
Viewed by 530
Abstract
Background/Objectives: Our study brings a new method to properly evaluating drug efficacy at the non-invasive in vitro level. Methods: In this work, the electrochemical mediator-free and reagent-free analysis of cell lines based on the registration of electrochemical profiles of membrane proteins was developed. [...] Read more.
Background/Objectives: Our study brings a new method to properly evaluating drug efficacy at the non-invasive in vitro level. Methods: In this work, the electrochemical mediator-free and reagent-free analysis of cell lines based on the registration of electrochemical profiles of membrane proteins was developed. We studied the specificity of cell lines Wi-38 and HepG2 and the toxic effects of drugs on cell-on-electrode systems. Results: A linear dependence of the peak current on the concentration of cells applied to the electrode in the range from 1 × 105 to 6 × 105 cells/electrode was registered (R2 0.932 for Wi-38 and R2 0.912 for HepG2). The water-soluble form of phosphatidylcholine (wPC) nanoparticles recommended for atherosclerosis treatment and prevention of cardiovascular diseases did not show a toxic effect on the human fibroblast cells, Wi-38, or the human hepatocellular carcinoma cells, HepG2, at sufficiently high concentrations (such as 0.1–1 mg/mL). The antitumor drug doxorubicin, at concentrations of 3 and 10 μg/mL, showed a pronounced toxic effect on the tested cell lines, where the percentage of living cells was 50–55%. Conclusions: A comparative analysis of the cytotoxicity of wPC (0.1–1 mg/mL) and doxorubicin (3–10 μg/mL) on the cell lines Wi-38 and HepG2 using the MTT test and electrochemical approach for the registration of cells showed their clear adequacy. Full article
(This article belongs to the Section Molecular and Translational Medicine)
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2 pages, 140 KiB  
Correction
Correction: Tao et al. Development of a Label-Free Electrochemical Aptasensor for the Detection of Tau381 and its Preliminary Application in AD and Non-AD Patients’ Sera. Biosensors 2019, 9, 84
by Dan Tao, Bingqing Shui, Yingying Gu, Jing Cheng, Weiying Zhang, Nicole Jaffrezic-Renault, Shizhen Song and Zhenzhong Guo
Biosensors 2025, 15(4), 234; https://doi.org/10.3390/bios15040234 - 7 Apr 2025
Viewed by 273
Abstract
In the original publication [...] Full article
14 pages, 4036 KiB  
Article
Warfarin Pharmacogenomics: Designing Electrochemical DNA-Based Sensors to Detect CYP2C9*2 Gene Variation
by Tiago Barbosa, Stephanie L. Morais, Eduarda Pereira, Júlia M. C. S. Magalhães, Valentina F. Domingues, Hygor Ferreira-Fernandes, Giovanny Pinto, Marlene Santos and Maria Fátima Barroso
Genes 2025, 16(4), 372; https://doi.org/10.3390/genes16040372 - 24 Mar 2025
Viewed by 785
Abstract
Background/Objectives: The CYP2C9 enzyme is involved in the metabolism of warfarin. The CYP2C9 gene harbors several single-nucleotide polymorphisms (SNPs), including CYP2C9*2 (rs1799853), which is known to affect warfarin’s therapeutic response. So, it is important to develop analytical tools capable of genotyping these SNPs [...] Read more.
Background/Objectives: The CYP2C9 enzyme is involved in the metabolism of warfarin. The CYP2C9 gene harbors several single-nucleotide polymorphisms (SNPs), including CYP2C9*2 (rs1799853), which is known to affect warfarin’s therapeutic response. So, it is important to develop analytical tools capable of genotyping these SNPs to adjust warfarin’s therapeutic outcomes. In this work, an electrochemical DNA-based sensor was constructed and optimized for the detection of the CYP2C9*2 polymorphism. Methods: Using bioinformatic database platforms, two 71 base pair DNA target probes with the polymorphic variants A and G were chosen and designed. A DNA-based sensor was composed by mercaptohexanol and the CYP2C9*2 DNA capture probe in a self-assembled monolayer connected to screen-printed gold electrodes. Two independent hybridization events of the CYP2C9*2 allele were designed using complementary fluorescein-labeled DNA signaling to improve selectivity and avoid secondary structures. Three human samples with the homozygous variant (G/G) and non-variant (A/A) and heterozygous (G/A) genotypes were amplified by PCR and then applied to the developed genosensor. Results: Chronoamperometry measurements were performed for both polymorphic probes. A calibration curve in the 0.25 to 2.50 nM (LOD of 13 pM) and another in the 0.15 to 5.00 nM range (LOD of 22.6 pM) were obtained for the homozygous non-variant and variant probes, respectively. This innovative tool was capable of identifying the hybridization reaction between two complementary strands of immobilized DNA, representing a genotyping alternative to the classical PCR methodology. Conclusions: The developed electrochemical DNA-based sensor was able to discriminate two synthetic SNP target sequences (Target-A and Target-G) and detect, with specificity, the three patients’ genotypes (G/G, G/A, and A/A). This tool is therefore a promising, sensitive, and cost-effective analytical way to determine and discriminate an individual’s genotype and predict the appropriate warfarin dose. Full article
(This article belongs to the Special Issue Genetics of Multifactorial Diseases: 2nd Edition)
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18 pages, 4954 KiB  
Article
In Situ Growth of Au NPs on Nitrogen-Doped Graphene Quantum Dots Decorated Graphene Composites for the Construction of an Electrochemical Immunosensor and Its Application in CEA Detection
by Zhengzheng Yan, Lujie Wang and Fei Yan
Molecules 2025, 30(6), 1347; https://doi.org/10.3390/molecules30061347 - 17 Mar 2025
Cited by 2 | Viewed by 1076
Abstract
Carcinoembryonic antigen (CEA) is an important tumor biomarker for the early clinical diagnosis of various cancers, and, therefore, the accurate and sensitive quantitative determination of CEA is of vital significance. In this study, we demonstrated the in situ growth of Au nanoparticles (AuNPs) [...] Read more.
Carcinoembryonic antigen (CEA) is an important tumor biomarker for the early clinical diagnosis of various cancers, and, therefore, the accurate and sensitive quantitative determination of CEA is of vital significance. In this study, we demonstrated the in situ growth of Au nanoparticles (AuNPs) on nitrogen-doped graphene quantum dots (N-GQDs) decorated reduced graphene oxide (rGO) nanocomposites by using simple drop-coating and electrochemical deposition methods. N-GQDs@rGO can be formed through the π–π stacking interaction and possesses a high specific surface area and many functional groups, providing lots of anchor sites (amino moieties in NGQDs) for the in situ electrochemical growth of AuNPs without the addition of reductants and protective agents. Such AuNPs/N-GQDs@rGO ternary nanocomposites combine the characteristics of three nanomaterials, showing a large surface area, excellent solubility, good conductivity, catalytic activity, a simple fabrication process, and notable stability, which are further used to construct a label-free electrochemical immunosensor for the determination of CEA. Under the optimized experimental conditions, the AuNPs/N-GQDs@rGO-based electrochemical immunosensor achieves a broad linear response, ranging from 1 pg/mL to 0.5 μg/mL and a low detection limit of 0.13 pg/mL. Moreover, the AuNPs/N-GQDs@rGO-based electrochemical immunosensor shows exceptional selectivity, anti-interference, and anti-fouling capabilities for the direct analysis of CEA amounts in fetal bovine serum samples, showing vast potential in the clinical screening of cancer. Full article
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17 pages, 56423 KiB  
Article
A Cost-Effective and Rapid Manufacturing Approach for Electrochemical Transducers with Magnetic Beads for Biosensing
by Milica Govedarica, Ivana Milosevic, Vesna Jankovic, Radmila Mitrovic, Ivana Kundacina, Ivan Nastasijevic and Vasa Radonic
Micromachines 2025, 16(3), 343; https://doi.org/10.3390/mi16030343 - 17 Mar 2025
Cited by 1 | Viewed by 1059
Abstract
Biosensors as advanced analytical tools have found various applications in food safety, healthcare, and environmental monitoring in rapid and specific detection of target analytes in small liquid samples. Up to now, planar electrochemical electrodes have shown the highest potential for biosensor applications due [...] Read more.
Biosensors as advanced analytical tools have found various applications in food safety, healthcare, and environmental monitoring in rapid and specific detection of target analytes in small liquid samples. Up to now, planar electrochemical electrodes have shown the highest potential for biosensor applications due to their simple and compact construction and cost-effectiveness. Although a number of commercially available electrodes, manufactured from various materials on different substrates, can be found on the market, their high costs for single use and low reproducibility persist as major drawbacks. In this study, we present an innovative, cost-effective approach for the rapid fabrication of electrodes that combines lamination of 24-karat gold leaves with low-cost polyvinyl chloride adhesive sheets followed by laser ablation. Laser ablation enables the creation of electrodes with customizable geometries and patterns with microlevel resolutions. The developed electrodes are characterized by cyclic voltammetry and electrochemical impedance spectroscopy, scanning electronic microscopy, and 3D profiling. To demonstrate the manufacturing and biosensing potential, different geometries and shapes of electrodes were realized as the electrochemical transducing platform and applied for the realization of magnetic bead (MB)-labeled biosensors for quantitative detection of food-borne pathogens of Salmonella typhimurium (S. typhimurium) and Listeria monocytogenes (L. monocytogenes). Full article
(This article belongs to the Section D3: 3D Printing and Additive Manufacturing)
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32 pages, 4902 KiB  
Review
Aspects of Electrochemical Biosensors Using Affinity Assays
by Thor Pedersen, Leonid Gurevich and Nils E. Magnusson
Biosensors 2025, 15(3), 166; https://doi.org/10.3390/bios15030166 - 4 Mar 2025
Cited by 5 | Viewed by 2138
Abstract
In recent decades, the utilization of biomarkers has gained increasing attention. The timely identification and quantification of proteins, nucleic acids, and small molecules associated with a medical condition, infection, or contaminant have become increasingly crucial across a variety of fields, including medicine, food [...] Read more.
In recent decades, the utilization of biomarkers has gained increasing attention. The timely identification and quantification of proteins, nucleic acids, and small molecules associated with a medical condition, infection, or contaminant have become increasingly crucial across a variety of fields, including medicine, food safety, and quality/environmental control. State-of-the-art biomarker detection methods predominantly rely on standard immunoassay techniques, requiring specialized laboratory equipment and trained personnel. This impedes the broad commercial implementation of biosensors in, e.g., Point-of-Care (PoC) settings where ease of operation, portability, and cost-efficiency are prioritized. Small, robust electrochemical biosensors are a promising alternative for analyzing biomarkers in complex samples within PoC environments. Therefore, creating and designing optimized sensing surfaces, immobilization strategies, and efficient signal generation are crucial for improving biosensor systems, which in turn can have real-world impact. In the present paper, we reviewed common electrode types and geometries used in electrochemical biosensors and the immobilization approaches, discussed the advantages and drawbacks of different electrochemical detection methods, and presented different labeling strategies for signal generation and enhancement. Full article
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