Search Results (449)

Grafting serves as a crucial propagation technique for superior Camellia oleifera varieties, where rootstock–scion compatibility significantly determines survival and growth performance. To systematically evaluate grafting compatibility in this economically important woody oil crop, we examined 15 rootstock–scion combinations using ‘Xianglin 210’ as the scion, assessing growth traits and conducting physiological assays (enzymatic activities of SOD and POD and levels of ROS and IAA) at multiple timepoints (0–32 days post-grafting). The results demonstrated that Comb. 4 (Xianglin 27 rootstock) exhibited superior compatibility, characterized by systemic antioxidant activation (peaking at 4–8 DPG), rapid auxin accumulation (4 DPG), and efficient sugar allocation. Transcriptome sequencing and WGCNA analysis identified 3781 differentially expressed genes, with notable enrichment in stress response pathways (Hsp70, DnaJ) and auxin biosynthesis (YUCCA), while also revealing key hub genes (FKBP19) associated with graft-healing efficiency. These findings establish that successful grafting in C. oleifera depends on coordinated rapid redox regulation, auxin-mediated cell proliferation, and metabolic reprogramming, with Comb. 4 emerging as the optimal rootstock choice. The identified molecular markers not only advance our understanding of grafting mechanisms in woody plants but also provide valuable targets for future breeding programs aimed at improving grafting success rates in this important oil crop. Full article

In recent years, growing concerns over environmental degradation and deepening awareness of the necessity of sustainable development have propelled green and low-carbon energy transition into a focal issue for both academia and policymakers. By decomposing energy transition into the transformation of energy structure and the upgrading of energy efficiency, this study investigates the impact and mechanisms of green finance on energy transition across 30 provinces (municipalities and autonomous regions) in China, with the exception of Tibet. In addition, the impact of climate change is incorporated into the analytical framework. Empirical results demonstrate that green finance development significantly accelerates energy transition, a conclusion robust to rigorous validation. Analysis of the mechanism shows that green finance promotes energy transition through the facilitation of technological innovation and the upgrade of industrial structures. Moreover, empirical evidence reveals that climate change undermines the promotional influence of sustainable finance on energy system transformation. The magnitude of this suppression varies nonlinearly across provincial jurisdictions with differing energy transition progress. Regional heterogeneity analyses further uncover marked discrepancies in climate–finance interactions, demonstrating amplified effects in coastal economic hubs, underdeveloped western provinces, and regions with mature eco-financial markets. According to these findings, actionable policy suggestions are put forward to strengthen green finance and accelerate energy transition. Full article

Fasciola hepatica remains a global health and economic concern, and treatment still relies heavily on triclabendazole. At the parasite–host interface, F. hepatica calcium-binding proteins (FhCaBPs) have a unique EF-hand/DLC-like domain fusion found only in trematodes. This makes it a parasite-specific target for small compounds and vaccinations. To enable novel therapeutic strategies, we report the first elevated-resolution structure of a full-length FhCaBP4. The apo structure was determined at 1.93 Å resolution, revealing a homodimer architecture that integrates an N-terminal, calmodulin-like, EF-hand pair with a C-terminal dynein light chain (DLC)-like domain. Structure-guided in silico mutagenesis identified a flexible, 16-residue β4–β5 loop (LTGSYWMKFSHEPFMS) with an FSHEPF core that demonstrates greater energetic variability than its FhCaBP2 counterpart, likely explaining the distinct ligand-binding profiles of these paralogs. Molecular dynamics simulations and AlphaFold3 modeling suggest that EF-hand 2 acts as the primary calcium-binding site, with calcium coordination inducing partial rigidification and modest expansion of the protein structure. Microscale thermophoresis confirmed calcium as the major ligand, while calmodulin antagonists bound with lower affinity and praziquantel demonstrated no interaction. Thermal shift assays revealed calcium-dependent stabilization and a merger of biphasic unfolding transitions. These results suggest that FhCaBP4 functions as a calcium-responsive signaling hub, with an allosterically coupled EF-hand–DLC interface that could serve as a structurally tractable platform for drug targeting in trematodes. Full article

Skeletal muscle development is a critical economic trait in livestock, governed by myogenic satellite cell regulation. Integrins mediate mechanical anchorage to the ECM and enable ECM–intracellular signaling. CIB2, as an EF-hand-domain protein involved in mechanotransduction, shows significant developmental regulation in goat muscle. Although the role of CIB2 in skeletal muscle growth is poorly characterized, we observed pronounced developmental upregulation of IB2 in postnatal goat muscle. CIB2 expression increased >20-fold by postnatal day 90 (P90) compared to P1, sustaining elevation through P180 (p < 0.05). Functional investigations indicated that siRNA-mediated knockdown of CIB2 could inhibit myoblast proliferation by inducing S-phase arrest (p < 0.05) and downregulating the expression of CDK4/Cyclin D/E. Simultaneously, CIB2 interference treatment was found to decrease the proliferative activity of goat myogenic satellite cells, yet it significantly promoted differentiation by upregulating the expression of MyoD/MyoG/MyHC (p < 0.01). Mechanistically, CTCF was identified as a transcriptional repressor binding to an intragenic region of the CIB2 gene locus (ChIP enrichment: 2.3-fold, p < 0.05). Knockdown of CTCF induced upregulation of CIB2 (p < 0.05). RNA-seq analysis established CIB2 as a calcium signaling hub: its interference activated IL-17/TNF and complement cascades, while overexpression suppressed focal adhesion/ECM–receptor interactions and enriched neuroendocrine pathways. Collectively, this study identifies the CTCF-CIB2–integrin α7β1–PI3K/AKT axis as a novel molecular mechanism that regulates the balance of myogenic fate in goats. These findings offer promising targets for genomic selection and precision breeding strategies aimed at enhancing muscle productivity in ruminants. Full article

Achieving carbon emission reductions in the residential building sector while maintaining economic growth represents a global challenge, particularly in rapidly developing regions with internal disparities. This study examines Jiangsu Province in eastern China—a economic hub with north-south development gradients—to develop an integrated framework for differentiated carbon reduction pathways. The methodology combines spatial autocorrelation analysis, logarithmic mean Divisia index (LMDI) decomposition, system dynamics modeling, and Tapio decoupling analysis to examine urban residential building emissions across three regions from 2016–2022. Results reveal significant spatial clustering of emissions (Moran’s I peaking at 0.735), with energy consumption per unit area as the dominant driver across all regions (contributing 147.61%, 131.82%, and 147.57% respectively). Scenario analysis demonstrates that energy efficiency policies can reduce emissions by 10.1% while maintaining 99.2% of economic performance, enabling carbon peak achievement by 2030. However, less developed northern regions emerge as binding constraints, requiring technology investments. Decoupling analysis identifies region-specific optimal pathways: conventional development for advanced regions, balanced approaches for transitional areas, and subsidies for lagging regions. These findings challenge assumptions about environment-economy trade-offs and provide a replicable framework for designing differentiated climate policies in heterogeneous territories, offering insights for similar regions worldwide navigating the transition to sustainable development. Full article

Hydrogen is being increasingly integrated into the international trade system as a clean and flexible energy carrier, motivated by the global energy transition and carbon neutrality objectives. The rapid expansion of the global hydrogen trade network has simultaneously exposed several sustainability challenges, including a centralized structure, overdependence on key countries, and limited resilience to external disruptions. Based on this, we develop a risk propagation model that incorporates the absorption capacity of nodes to simulate the propagation of supply shortage risks within the global hydrogen trade network. Furthermore, we propose a composite sustainability index constructed from structural, economic, and environmental resilience indicators, enabling a systematic assessment of the network’s sustainable development capacity under external shock scenarios. Findings indicate the following: (1) The global hydrogen trade network is undergoing a structural shift from a Western Europe-dominated unipolar configuration to a more polycentric pattern. Countries such as China and Singapore are emerging as key hubs linking Eurasian regions, with trade relationships among nations becoming increasingly dense and diversified. (2) Although supply shortage shocks trigger structural disturbances, economic losses, and risks of carbon rebound, their impacts are largely concentrated in a limited number of hub countries, with relatively limited disruption to the overall sustainability of the system. (3) Countries exhibit significant heterogeneity in structural, economic, and environmental resilience. Risk propagation demonstrates an uneven pattern characterized by hub-induced disruptions, chain-like transmission, and localized clustering. Accordingly, policy recommendations are proposed, including the establishment of a polycentric coordination mechanism, the enhancement of regional emergency coordination mechanisms, and the advancement of differentiated capacity-building efforts. Full article

As global climate change intensifies and the transition to clean energy accelerates, lithium-ion batteries—critical components of electric vehicles—are becoming increasingly vital in international trade networks. This study investigates the structural evolution and determinants of the electric vehicle lithium-ion battery trade network among European Union (EU) member states from 2012 to 2023, employing social network analysis and the multiple regression quadratic assignment procedure method. The findings demonstrate the transformation of the network from a centralized and loosely connected structure, with Germany as the dominant hub, to a more interconnected and decentralized system in which Poland and Hungary emerge as the leading players. Key network metrics, such as the density, clustering coefficients, and average path lengths, reveal increased regional trade connectivity and enhanced supply chain efficiency. The analysis identifies geographic and economic proximity, logistics performance, labor cost differentials, energy resource availability, and venture capital investment as significant drivers of trade flows, highlighting the interaction among spatial, economic, and infrastructural factors in shaping the network. Based on these findings, this study underscores the need for targeted policy measures to support Central and Eastern European countries, including investment in logistics infrastructure, technological innovation, and regional cooperation initiatives, to strengthen their integration into the supply chain and bolster their export capacity. Furthermore, fostering balanced inter-regional collaborations is essential in building a resilient trade network. Continued investment in transportation infrastructure and innovation is recommended to sustain the EU’s competitive advantage in the global electric vehicle lithium-ion battery supply chain. Full article

The agglomeration characteristics of innovation spaces reflect the intrinsic mechanisms of regional resource integration and collaborative innovation. Investigating the contributions of influencing factors to innovation space agglomeration and their spatial differentiation has significant implications for improving urban innovation quality. Taking the Nanjing central urban area as a case study, this research applied gradient boosting regression trees (GBRT) and multiscale geographically weighted regression (MGWR) models to explore the contributions of influencing factors to innovation space agglomeration and its spatial differentiation. Findings demonstrated that (1) Innovation platforms and patents emerged as the most significant driving factors, collectively accounting for 54.8% of the relative contributions; (2) The contributions of influencing factors to innovation space agglomeration exhibited marked nonlinear characteristics, specifically categorized into five distinct patterns: Sustained Growth Pattern, Growth-Stabilization Pattern, Growth-Decline Pattern, Global Stabilization Pattern, and Global Decline Pattern. The inflection thresholds of marginal effects across factors ranged from approximately 12% to 55% (e.g., 40% for metro stations, 13% for integrated commercial hubs); (3) Each influence factor’s contribution mechanism showed pronounced spatial heterogeneity across different regions. Based on these discoveries, governments should optimize innovation resource allocation according to regional characteristics and enhance spatial quality to promote efficient resource integration and transformation. This research provides a novel perspective for understanding innovation space agglomeration mechanisms and offers actionable references for urban policymakers to implement context-specific innovation economic development strategies. Full article

The agricultural products trade network is essentially a complex adaptive system formed by nonlinear interactions between countries. Based on the complex system theory, this study reveals the dynamic self-organization law of the RCEP regional agricultural products trade network by using the panel data of RCEP agricultural products export trade from 2000 to 2023, combining social network analysis (SNA) and the temporal exponential random graph model (TERGM). The results show the following: (1) The RCEP agricultural products trade network presents a “core-edge” hierarchical structure, with China as the core hub to drive regional resource integration and ASEAN countries developing into secondary core nodes to deepen collaborative dependence. (2) The “China-ASEAN-Japan-Korea “riangle trade structure is formed under the RCEP framework, and the network has the characteristics of a “small world”. The leading mode of South–South trade promotes the regional economic order to shift from the traditional vertical division of labor to multiple coordination. (3) The evolution of trade network system is driven by multiple factors: endogenous reciprocity and network expansion are the core structural driving forces; synergistic optimization of supply and demand matching between economic and financial development to promote system upgrading; geographical proximity and cultural convergence effectively reduce transaction costs and enhance system connectivity, but geographical distance is still the key system constraint that restricts the integration of marginal countries. This study provides a systematic and scientific analytical framework for understanding the resilience mechanism and structural evolution of regional agricultural trade networks under global shocks. Full article

The European Union’s developing rural development plan places digital and precision agriculture at the centre of agricultural modernisation and economic development. This article examines how agricultural practices in rural EU regions are being influenced by smart technology, such as drones, IoT sensors, satellite-based research, and AI-driven platforms, through an analysis of recent data from sources across the European Union. This study applies a mixed-methods approach, combining quantitative analysis of strategic policy documents and EU databases, to evaluate the ways in which precision agriculture reduces input consumption, increases productivity, reduces labour shortages and rural area depopulation, and improves sustainability. By investing in infrastructure, developing communities for data exchange, and organising training for farmers, European policies such as the Strategic Plans of the Common Agricultural Policy (CAP), the SmartAgriHubs initiative, and the AgData program actively encourage the transition to digital agriculture. Cyprus is analysed as a case study to show how targeted investments and initiatives supported by the EU can help smaller countries, with limited natural resources, to realise the benefits of digital transformation in agriculture. A special focus is placed on how solutions adapted to agro-climatic and socioeconomic conditions can contribute to strengthening the competitiveness of the agricultural sector, attracting young people to get involved in this field and opening up new economic opportunities. The results of previous research indicate that digital agriculture not only improves productivity but also proves to be a strategic mechanism for attracting and retaining young people in rural areas. Thus, this work additionally contributes to the broader goal of the European Union—the development of smart, inclusive, and sustainable rural areas, in which digital technologies are not only seen as tools for efficiency but also as key means for integrated and long-term rural development. Full article

The future of marine sustainability depends on public understanding and trust in the policy recommendations that emerge from scientific research. For common pool marine resource decisions made by the people who depend on these resources for their food, employment, and economic future, understanding the current status of these marine systems and change is essential to ensure these resources will persist into the future. As such, the informal learning infrastructure is essential to increasing marine science literacy in a changing world. This mixed-methods research study analyzed the distribution and accessibility of marine science education and research across Alaska’s five geographic regions. Using the PRISMA framework, we synthesized data from 198 institutions and analyzed peer-reviewed literature on marine ecosystems to identify geographic and thematic gaps in access to informal science learning and research focus. In parallel, we undertook geospatial analysis and resource availability to describe the distribution of resources, types of informal learning infrastructure present across the state, regional presence, and resources to support informal marine science learning opportunities. Findings from this multifactor research revealed a concentration of resources in urban hubs and a lack of consistent access to learning resources for rural and Indigenous communities. The configurative literature review of 9549 publications identified topical underrepresentation of the Bering Sea and Aleutian Islands, as well as a lack of research on seabirds across all regions. Considered together, these results recommend targeted investments in rural engagement with marine science programming, culturally grounded partnerships, and research diversification. This review concludes that disparities in learning resource support and government-funded priorities in marine wildlife research have created conditions that undermine the local people’s participation in the sustainability of sensitive resources and are likely exacerbating declines driven by rapid change in Arctic and sub-Arctic waters. Full article

This study examines the impact of the Beijing–Tianjin–Hebei (BTH) coordinated development policy on the regional industrial network structure, with a focus on the significance of electricity consumption data in social network analysis (SNA). Utilizing a gravity model integrated with electricity consumption data, this research employs centrality analysis and Lambda analysis to compare changes in the steel industry network before and after policy implementation. The findings reveal that traditional models relying solely on indicators such as population and Gross Domestic Product (GDP) fail to comprehensively capture regional economic linkages, whereas incorporating electricity consumption data enhances the model’s accuracy in identifying core nodes and latent connections. Post policy implementation, the centrality of Beijing and Tianjin increased significantly, reflecting their transition from production hubs to centers for research and development (R&D) and management, while Shijiazhuang’s pivotal role diminished. This study also uncovers a “core–periphery” structure in the BTH urban network, where core cities (Beijing, Tianjin, and Shijiazhuang) dominate resource allocation and information flow, while peripheral cities exhibit uneven development. These results provide a scientific basis for optimizing regional coordinated development policies and underscore the critical role of electricity consumption data in refining regional economic analysis. Incorporating electricity consumption data into the gravity model significantly enhances its explanatory power by capturing hidden economic ties and improving policy evaluation, offering a more accurate and dynamic assessment of regional industrial linkages. Full article

Apple pomace, a by-product from the production of concentrated juice, is a major contributor to global food waste. Despite its beneficial nutritional profile, apple pomace is predominantly disposed of in landfills. Rapid fermentation and spoilage caused by microorganisms are compounding factors in this demise, despite significant research into upcycling strategies. Thus, there is an unmet need for economical approaches that allow for the preservation of pomace during storage and transportation to centralized processing facilities from regional hubs. To address this challenge, we investigated the potential of different preservatives for preventing microbial growth and the spoilage of apple pomace, including antimicrobials (natamycin and iodine), polysaccharides (chitosan and fucoidan), and acetic acid. Spread plates for total microbial and fungal counts were employed to assess the effectiveness of the treatments. High concentrations (10,000 ppm) of chitosan were effective at reducing the microbial load and inhibiting growth, and in combination with antimicrobials, eliminated all microbes below detectable levels. Nevertheless, acetic acid at an equivalent concentration to commercial vinegar displayed the highest economic potential. Apple pomace submerged in 0.8 M acetic acid (3 kg pomace per liter) resulted in a five-log reduction in the microbial colony-forming units (CFUs) out to 14 days and prevented fermentation and ethanol production. These results provide a foundation for the short-term storage and preservation of apple pomace that could contribute to its upcycling. Full article

As China’s economy grows and travel demand increases, its aviation market has evolved to become the second-largest in the world. This study presents a pioneering analysis of China’s aviation network evolution (1990–2024) by integrating temporal dynamics into a network density matrix theory, addressing critical gaps in prior static network analyses. Unlike conventional studies focusing on isolated topological metrics, we introduce a triangulated methodology: ① a network sequence analysis capturing structural shifts in degree distribution, clustering coefficient, and path length; ② novel redundancy–entropy coupling quantifying complexity evolution beyond traditional efficiency metrics; and ③ economic-network coordination modeling with spatial autocorrelation validation. Key innovations reveal previously unrecognized dynamics: ① Time-embedded density matrices ($\rho$) demonstrate how sparsity balances information propagation efficiency ($\eta$) and response diversity, resolving the paradox of functional yet sparse connectivity. ② Redundancy–entropy synergy exposes adaptive trade-offs. Entropy ($H$) rises 18% (2000–2024), while redundancy ($R$) rebounds post-2010 (0.25→0.33), reflecting the strategic resilience enhancement after early efficiency-focused phases. ③ Economic-network coupling exhibits strong spatial autocorrelation ($Moran’sI>0.16$, $p<0.05$), with eastern China achieving “primary coordination”, while western regions lag due to geographical constraints. The empirical results confirm structural self-organization. Power-law strengthening, route growth exponentially outpacing cities, and clustering ($C$) rising 16% as the path length ($L$) increases, validating the hierarchical hub formation. These findings establish aviation networks as dynamically optimized systems where economic policies and topological laws interactively drive evolution, offering a paradigm shift from descriptive to predictive network management. Full article

Micro, Small, and Medium Enterprises (MSMEs) are vital drivers of economic growth in Kenya, yet they face persistent barriers, including limited capacity, financial exclusion, and weak market integration. This study assessed the potential of universities as strategic hubs for MSME capacity building through a collaborative initiative between Egerton University and the KCB Foundation. Using the International Labour Organization’s Start and Improve Your Business (SIYB) methodology, 481 entrepreneurs from Egerton, Njoro, and Gilgil were trained in a business development bootcamp. This study evaluated the training effectiveness, participant demographics, confidence in skill application, networking outcomes, and satisfaction levels. The results showed high participant confidence (over 95% across all regions), strong financial management uptake (85%), and mobile banking adoption (70%). Gilgil led in inclusivity and peer engagement, while Njoro showed stronger gender representation. However, logistical challenges caused 25% absenteeism in rural areas, and only 23% accessed post-training mentorship. These findings underscore the transformative role of HEIs in fostering sustainable entrepreneurship through localized, inclusive, and industry-aligned training. Policy recommendations include hybrid delivery models, tiered curricula for diverse skill levels, and institutionalized mentorship through public–private partnerships. This case demonstrates the value of embedding entrepreneurship support within university mandates to advance national MSME development agendas. Full article