Search Results (9,111)

This article presents the design and development of an interactive architecture oriented toward the management of traditional vallenato, a musical genre recognized as an Intangible Cultural Heritage of Humanity by UNESCO. Architecture combines the principles of contextual awareness and the use of massive open online courses (MOOCs) to face the current challenges of preservation, dissemination, and teaching of this cultural expression, threatened by commercialization and the loss of its traditional roots. Through a modular structure, adaptive technological tools are integrated to capture, process, and use contextual information, personalizing learning experiences and strengthening the link between communities and their cultural heritage. The proposal consists of several functional layers, including context management, user profiles, educational resources, and a persistence unit, each designed to ensure the interoperability and sustainability of cultural data. In addition, the capacity of architecture to be used in other cultural contexts is highlighted, expanding its impact on different artistic manifestations and heritages worldwide. This article includes a comparative analysis with other existing models, highlighting the advantages of this solution in terms of customization and adaptability. Finally, opportunities for improvement and expansion are explored, as well as the pending challenges in the implementation of this technological tool in educational and cultural environments. Full article

Public participation in planning, though a foundational democratic principle, faces persistent implementation challenges across diverse planning systems. This paper examines participatory planning practice in Ireland and Serbia—two countries representing distinct planning traditions (discretionary and conformance-based, respectively) yet confronting shared structural pressures. Through comparative analysis of four local land use planning instruments (the Development Plan and Local Area Plan in Ireland; the Municipal Spatial Plan and General Regulation Plan in Serbia), the study investigates how institutional design and legislative frameworks shape the depth and quality of participatory practice. Methodologically, the research triangulates statutory regulations, public hearing documentation, and non-statutory participation records across two planning scales (county/municipal and local/sub-municipal). A four-dimensional analytical framework—informing, consultation, collaboration, and monitoring—guides the systematic comparison of participatory mechanisms across the selected cases. Findings reveal that, while both systems remain predominantly at the informing and consultation levels, critical differences emerge in how participation is structured and documented in institutional practice. Ireland’s discretionary system enables multi-channel information dissemination, feedback-oriented consultation, and non-statutory collaborative experimentation beyond legal minimums. Serbia’s conformance-based system confines participation largely to statutory procedures, with objection-based consultation and limited collaborative mechanisms, though distinctive features, such as the public hearing session, provide direct opportunities for deliberation absent in the Irish context. The study contributes to European comparative planning scholarship by demonstrating that participatory depth is shaped less by the formal existence of legal provisions than by the interplay between institutional design, procedural arrangements, transparency, and responsiveness. Full article

The digital preservation and dissemination of historical and cultural heritage is a pivotal area at the intersection of digital humanities and geographic information science. To address the challenges of multi-source heterogeneity, limited dimensionality, and inadequate public engagement, this study designed and implemented an interactive visualization platform using modern Web technologies. Taking the Leshan Confucian Temple (religious heritage) and the former site of Wuhan University (educational heritage) as case studies, the platform integrates four types of heterogeneous data (geospatial coordinates, architectural attributes, visitor behavioral records, and multimedia imagery) into a unified spatiotemporal information model. Core technical implementations are built upon a lightweight front-end stack including the Gaode Map JavaScript API for geographic visualization, ECharts for dynamic statistical charting, and the Tailwind CSS framework for a fully responsive front-end interface. Key interactive features encompass linked map markers with contextual information windows, user-driven chart filtering, and paginated loading of cultural relic cards. Evaluation results demonstrate that the platform achieves cross-device response delay ≤3 s, supports spatially grounded, dynamic, and presentation of cultural heritage information, and attains a System Usability Scale (SUS) score of 82.5. This work offers a lightweight, scalable technical solution for advancing digital recording and public communication of historical and cultural heritage, while contributing to the theoretical discourse on spatial narrative and multi-source data integration in digital humanities. Full article

The construction industry is one of the major sources of carbon emissions, and green retrofitting of buildings is an effective pathway to promoting sustainable development in the sector. However, existing research and implementation strategies often struggle to reconcile the needs of governments, businesses, and residents. Therefore, this study proposes a comprehensive research framework that employs bibliometric and text analysis methods to examine implementation barriers in retrofitting projects across four dimensions: policy, cost, technology, and resident satisfaction. The results indicate that retrofitting costs are the primary factor, while technology is a secondary factor. Furthermore, existing policies feature vague technical standards, insufficient incentives, and a lack of differentiation. Conflicts of interest and challenges regarding cost allocation persist throughout the renovation life cycle. Decision-support tools and renovation technologies face limitations and issues regarding applicability. Residents face constraints from multiple factors, including their knowledge base and economic capacity. Based on these findings, the government urgently needs to improve a differentiated policy system and encourage technological R&D and knowledge dissemination. Enterprises must actively respond to policies and optimize their technologies and management practices. Residents need to enhance their energy-saving awareness, participate in retrofitting efforts, and improve their energy consumption behaviors. Full article

The increasing integration of Virtual Reality (VR) technologies in cultural and historical contexts has significantly transformed the way heritage and legacy are preserved, studied, and experienced. This study provides a bibliometric analysis of the current research landscape surrounding the use of VR in heritage and legacy research. The results obtained highlight a research environment dominated by European institutions—primarily Italian and Spanish—complemented by Asian and French contributions that demonstrate a trend toward progressive internationalization. This field of research combines immersive technologies, photogrammetry for 3D digitization and user-centered designs, moving from conservationist approaches to holistic approaches that prioritize accessibility, educational dissemination and tourism. The results reveal a duality between digital documentation and immersive experience, while, among the countries with the most World Heritage sites, Italy leads in terms of quantity and average citations, China in terms of total volume, and Spain shows underutilized bibliometric potential despite its rich historical heritage. This analysis aims to trace the evolution of this field of research, uncover gaps, and suggest directions for future work that leverages virtual reality to safeguard and disseminate cultural heritage in an immersive and impactful way. Full article

Background/Objectives: Myxoid liposarcoma (MLS) is a malignant soft-tissue tumor and the second-most common subtype of liposarcoma, often occurring in the lower limbs of middle-aged patients. Case presentation: A 38-year-old male patient presented to the ultrasound outpatient clinic with a large mass in the right femoral region. It has been present for 15 years and mostly stable in size. Over the last two years, it has been slowly increasing in size, more rapidly in the previous 10 months, and has started to limit his range of motion. After multiparametric ultrasound and magnetic resonance imaging evaluation, the proposed diagnosis was myxoid liposarcoma. Following imaging workup, the patient was referred to the tertiary sarcoma center, where a biopsy was performed, and pathohistological diagnosis was low-grade myxoid liposarcoma. Contrast-enhanced computed tomography (CT) evaluation of the thorax, abdomen, and pelvis showed no signs of dissemination, and CT angiography showed no signs of vessel infiltration. Plastic surgery and vascular surgery specialists performed the extirpation of the mass with the partial resection of the adjacent sartorius muscle and the complete resection of the great saphenous vein. Subsequent pathohistological analysis of the mass and local lymph nodes showed clear surgical margins and no lymphatic or vascular invasion. The patient is currently under regular surveillance by an oncology specialist and awaiting adjuvant radiotherapy. Conclusions: A multidisciplinary approach is essential in the management of patients with MLS, as it provides a tailored, individualized assessment from diagnosis through treatment to ensure the best possible outcome. Full article

Background: Diffuse large B-cell lymphoma (DLBCL) is the most common subtype of non-Hodgkin lymphoma, constituting an important public health problem. Although curable, it presents a widely variable prognosis. The main tool used for prognostic stratification in DLBCL is the International Prognostic Index (IPI), which does not consider crucial biological variables for understanding its prognostic heterogeneity. Cell adhesion molecules (CAMs) play a central role in cancer biology and can be evaluated in affected tissues or in plasma, in soluble forms (sCAMs). CAMs promote proliferation, survival, and dissemination of malignant cells. Although extensively studied in solid tumors, their role remains unclear in hematological malignancies, particularly in DLBCL. Methods: This is a prospective and longitudinal study involving 87 newly diagnosed DLBCL (ND-DLBCL) patients aiming to quantify plasma levels of sCAMs (sICAM-1, sVCAM-1, sP-selectin, and sE-cadherin) at diagnosis and assessing its potential prognostic impact, as well as establishing clinical-biological associations. Results: Plasma quantification of sICAM-1, sVCAM-1, and sP-selectin did not present prognostic impact in DLBCL. However, continuous increases in sE-cadherin levels, as well as sE-cadherin ≥ 126.55 ng/mL were associated with lower response rates to R-CHOP regimen, higher frequency of recurrence following first-line therapy, and shortened survival. Additionally, sE-cadherin concentration ≥ 126.55 ng/mL was an independent predictor related to decreased overall survival. Conclusion: sE-cadherin measured at diagnosis has emerged as a new prognostic biomarker able to predict response, relapse and survival in ND-DLBCL. Full article

The preservation of tacit knowledge embedded in Southern Thai textiles remains a significant challenge because much of this knowledge is dispersed across private households and is inadequately supported by conventional object-centered documentation systems. This study developed a crowdsourcing-based digital storytelling platform that enabled communities to document, organize, and disseminate knowledge related to Southern Thai textile heritage. The platform integrated community participation, structured narrative authoring, and knowledge organization within a socio-technical system designed for cultural heritage documentation. To guide its development, the study proposed the Crowdsourced-Storytelling Intangible Cultural Heritage Framework (CS-ICH Framework) and operationalizes it through requirements analysis, iterative prototyping, and empirical user evaluation. The evaluation results indicated high levels of user satisfaction and positive user perceptions regarding knowledge accessibility, content organization, and the platform’s support for heritage preservation. These findings suggested that participatory digital platforms can effectively facilitate the documentation and dissemination of locally embedded cultural knowledge that is difficult to capture through conventional institutional systems. This study contributed to digital heritage research by providing a potentially transferable framework and design principles for integrating crowdsourcing and digital storytelling within platforms designed to preserve and transmit intangible and community-held cultural heritage. Full article

Cancer cell dormancy is a clinically consequential yet computationally under-defined phenomenon characterised by reversible growth arrest and delayed disease recurrence. Although advances in single-cell and multi-omic profiling have improved detection of dormant and persister populations, their molecular identity and dynamical behaviour remain difficult to resolve. In this review, we examine how computational methods have been applied to infer dormant cell identity, heterogeneity, microenvironmental regulation, state transitions, and reactivation dynamics. We highlight how single-cell transcriptomics, lineage tracing, spatial profiling, and integrative multi-omic analyses reveal substantial context-dependent variability, undermining the notion of a universal dormancy signature. We further discuss emerging mathematical and statistical frameworks to model the awakening from dormancy, alongside approaches linking dormancy-associated features to clinical outcomes. Recurring challenges include fragmented operational definitions, rare-state detection, cross-study incompatibility, and the use of snapshot data to interrogate inherently temporal processes. We argue that progress will depend on computational frameworks that treat dormancy as a dynamic, multi-scale systems problem rather than a static cell-type classification task. Full article

Although neurons are not productively infected by HIV-1, the envelope glycoprotein gp120, detectable in cerebrospinal fluid independently of active viral replication, gains intraneuronal access via lipid raft-mediated endocytosis, macropinocytosis, and retrograde axonal transport, contributing to persistent neurobiological dysfunction within the central nervous system. Once internalized, gp120 is associated with neuronal dysfunction involving convergent pathways, including excitotoxic calcium dysregulation, mitochondrial and metabolic failure, and inflammatory and senescence-associated amplification. These pathways converge on suppression of CREB and BDNF signaling, dismantling the transcriptional and neurotrophic programs required for synaptic maintenance and cognitive resilience. Extracellular vesicle-mediated dissemination and microRNA reprogramming extend gp120-associated neurobiological effects beyond sites of receptor engagement, while gut-derived metabolites, particularly quinolinic acid, lower the excitotoxic threshold through synergistic activation of NMDA receptors. Together, these mechanisms define HAND as a network disorder in which gp120 contributes to persistent neurocognitive dysfunction beyond active viral replication, identifying convergent therapeutic nodes where combination strategies targeting excitotoxicity, mitochondrial dysfunction, and neuroinflammation offer the most promising path toward durable neuroprotection. Full article

Bacterial persistence, a non-heritable high-antibiotic-tolerance phenotype, is a key driver of recurrent clinical infections and antibiotic treatment failure. The pheromone-responsive pCF10 plasmid in Enterococcus faecalis (E. faecalis) mediates antibiotic resistance gene dissemination, but its role in bacterial persister formation remains unclear. This study systematically investigated the regulatory role of pheromone cCF10 in the persister phenotype of pCF10-carrying E. faecalis and its underlying molecular mechanisms. We confirmed that cCF10 enhanced persistence against levofloxacin in OG1RF (pCF10), with the persister frequency increasing from 0.291% to 16.466% upon treatment. Transcriptomic analysis revealed that cCF10 activated the (p)ppGpp-mediated stringent response and downregulated the expression of genes associated with energy-intensive pathways, including those involved in DNA repair, protein folding, and respiration. Concurrently, cCF10 enhanced the expression of genes related to biofilm formation and cell lysis resistance and downregulated components of its own sensing and uptake systems. These findings demonstrate that cCF10 induces transcriptional reprogramming associated with increased persister formation in E. faecalis carrying the pCF10 plasmid and identify potential targets within the stringent response and associated metabolic pathways for the development of anti-persister strategies. Full article

Chicken manure and its potential to contaminate water systems through the dispersal of pathogenic bacteria are major concerns in environmental and public health. In this study, a metagenomic analysis was employed to systematically identify and compare bacterial assemblages in chicken manure (CM) and in a contaminated sample of chicken manure wastewater (CMW). Whole DNA was extracted from CM and CMW, followed by whole-genome shotgun sequencing; data analysis was done using online Galaxy software (ver. 26.0.1.dev1). Metagenomic analysis reveals a complex One Health challenge. Data showed that CM and CMW are different in their microbiota, as indicated by a distinct separation of beta diversity values and limited overlapping of species between sample types. In the current study, we found a greatly significant common functional set of adapted bacterial masses, including major pathogenic bacterial groups as well as opportunistic and environmental bacterial species, indicative of a direct contamination from CM and CMW. Notably, in both CM and CMW, a plethora of opportunistic, enteric, and environmental pathogens like Escherichia coli, Salmonella enterica, and Acinetobacter baumannii were found, coupled with an indication of a direct functional flow between both ecosystems as tangled reservoirs. Chicken manure samples showed differences in taxonomic composition and inferred functional profiles at the time of sampling: CM1 was pathogen-enriched, CM2 exhibited strong nitrogen-supportive metabolism, CM3 was dominated by fiber-degrading decomposers, and CM4 showed high methane-producing potential with environmental risk. Such findings underscore the raising of chickens as a potential source of harmful bacteria for the environment. It is important to note that this study represents a preliminary investigation with certain limitations, including the absence of biological replicates, lack of temporal sampling, and limited capacity to infer dynamic ecological interactions. Yet this metagenomic report is more about describing the taxonomy and functional potential of the bacteria, rather than discussing the actual ecological processes of these microorganisms in the environment. Future studies will be required to explore these aspects. Full article

The current study assesses farmers’ knowledge of land preparation for potato planting, potato crop inputs and production practices, healthy, high-quality, and disease-free potato seeds, and their perceptions of agricultural extension services. A stratified random sample of 262 potato farmers from Hail, Qassim, Tabuk, Riyadh, and Al-Jawf was selected according to the Yamane equation. The number of completed and validated questionnaires was 231. Findings revealed that the majority of respondents have strong knowledge of land preparation for potato planting, potato crop production practices, and the selection of healthy, high-quality, and disease-free potato seeds. Moreover, the majority of farmers agreed with the role of agricultural extension in disseminating knowledge of sustainable agricultural potato cultivation practices. Farmers’ age and education level significantly influenced their knowledge of selecting healthy, high-quality, and disease-free potato seeds. Farming experience significantly influenced knowledge of land preparation for potato planting, crop inputs, production practices, and high-quality potato seeds. Monthly farm income and income from potato farms significantly influenced farmers’ knowledge of land preparation for potato planting, potato crop inputs, production practices, and healthy, high-quality, and disease-free potato seed selection. Regarding agricultural extension services, education level, monthly farm income, and income from potato crops influenced farmers’ perceptions of their effectiveness in disseminating information about sustainable agricultural practices. Moreover, the findings of multiple regressions indicate that farmers’ income from potato farms affects their knowledge of land preparation and healthy, high-quality, and disease-free potato seeds. Farming experience and income from potato farms significantly affect farmers’ knowledge of crop inputs and production practices. Education and monthly farm income affect farmers’ perceptions of agricultural extension services. The study emphasizes the need for educational programs, training, and workshops under the supervision of the Agricultural Extension Department to enhance farmers’ knowledge of sustainable potato cultivation practices. The government should provide subsidized advanced agricultural technologies in the study area, thereby enhancing crop production and livelihoods. Support from the government and the extension department would help reduce potato imports and the economic burden by improving local potato production. Full article

Microplastics (MPs) are pervasive environmental contaminants detected in terrestrial, aquatic, and human systems. Emerging evidence indicates that MPs interact with microbiota through biofilm formation, induction of oxidative stress, enrichment of antibiotic resistance genes (ARGs), and disruption of short-chain fatty acid metabolism, leading to dysbiosis and altered host immune responses. These interactions contribute to dysbiosis, altered immune responses, and increased dissemination of ARGs, which pose health risks. This review synthesizes current knowledge on mechanisms of microplastic–microbiota interactions, highlighting evidence from in vitro, in vivo, and environmental studies. We discuss methodological challenges, including variability in particle types, concentrations, aging, and analytical approaches. Recent advances in multi-omics techniques provide deeper mechanistic understanding and reveal functional consequences of MP exposure. We outline key knowledge gaps and propose future research directions to assess the impact of microplastic exposure on ecosystems and human health. Full article

In today’s densely populated and technology-driven smart cities, natural and human-made disasters increasingly threaten the resilience of communication infrastructures, creating critical challenges for maintaining reliable connectivity. The failure of conventional networks during crises significantly hampers emergency response, coordination, and information dissemination. To address these challenges, this paper presents Weighted Average Algorithm-based Clustering and Routing (WAA-CR), a novel, secure, and adaptive UAV-based framework for disaster response and recovery. WAA-CR integrates three key components: shelters or Ground Control Stations (GCSs) as communication anchors and support hubs, survivable clustering and routing using a WAA-based metaheuristic optimizer, and secure and trustworthy drone communication enabled by a lightweight trust evaluation mechanism, and authentication model. The framework formulates a multi-objective optimization model that simultaneously minimizes the number of active UAVs and routing cost, while maximizing trust, communication reliability, and coverage. Cluster head (CH) election and routing decisions are guided by a composite fitness function that considers residual energy, link stability, mobility, and dynamic trust scores. Additionally, an adaptive maintenance mechanism enables dynamic reconfiguration to handle CH failures, trust degradation, or mobility-driven topology changes. Extensive simulations conducted in MATLAB R2020ademonstrate that WAA-CR significantly outperforms existing baseline FANET protocols in terms of energy efficiency, cluster stability, trust accuracy, and end-to-end delivery performance. These results validate the proposed framework’s effectiveness in building resilient, scalable, and secure UAV-based communication networks for post-disaster environments. Full article